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Son, Hyunjong,Lee, Hye Young,Lim, Jung Mi,Kang, Dongmin,Han, Won Seok,Lee, Shim Sung,Jung, Jong Hwa Wiley (John WileySons) 2010 Chemistry Vol.16 No.38
<P>A new fluoro-chromogenic chemosensor based on BODIPY-functionalized Fe(3)O(4)@SiO(2) core/shell nanoparticles 1 has been prepared. Chemosensor 1 exhibits a high affinity and selectivity for Pb(2+) over competing metal ions tested. Moreover, confocal microscopy, and flow cytometry experiments established that 1 can be used for detecting Pb(2+) levels within living cell.</P>
Keun Young Huh(Keun Young Huh),Hyuncheol Heo(Hyuncheol Heo),Hyunjong Jung(Hyunjong Jung),Inhea Kim(Inhea Kim) 인간식물환경학회 2022 인간식물환경학회지 Vol.25 No.5
Background and objective: This study was conducted to analyze users’ behavior, recognition, and satisfaction toward forest trails and exercise facilities in urban mountainous forests, Jinju City, South Korea. Methods: Users’ characteristics, visit motives, and satisfaction with forest trails and exercise facilities in three urban mountainous forests were surveyed from September to October 2020. The data of 300 respondents were analyzed using frequency, descriptive, and regression analysis. Results: There was a high percentage of users aged 51 or older (62.0%). Most users visited regardless of weekdays or weekends, every day or 2-3 times a week, and stayed for 1-2 hours. Most were using the forest trails and outdoor fitness facilities. Hiking or walking showed the highest frequency in visit motives, followed by using outdoor fitness facilities. Overall satisfaction with forest trails was high at 3.74-3.93 on a 5-point Likert scale, which was affected by the landscape and environment of small private arable lands around forest trails and convenient facilities such as handrails and information boards. Overall satisfaction with outdoor fitness facilities was 3.34-3.62, which was affected by harmony with forest landscape and facility maintenance. Overall satisfaction with indoor badminton courts was 3.01-3.08, which was affected by harmony with forest landscape. Conclusion: For improving overall satisfaction, it is crucial to maintain and repair the width and surface of forest trails, but renovating the landscape and environment of small private arable lands and exercise facilities may be more critical. It is desirable to improve and maintain the function and landscape of outdoor fitness facilities and to relocate indoor badminton courts to the outskirts or boundaries of the mountainous forests. To provide various leisure and recreation activities, the creation of a forest bathing place will be first necessary, followed by a family recreation place and outdoor learning classroom.
A computed tomography-based analysis of the structure of the mandible according to age and sex
Jung, Soyeon,Yun, Hyunjong,Chung, Chul Hoon,Kim, Kuylhee,Chang, Yongjoon Korean Cleft Palate-Craniofacial Association 2022 Archives of Craniofacial Surgery Vol.23 No.3
Background: The primary objectives of mandibular surgery are to achieve optimal occlusion, low sensory disturbance, and adequate fixation with early movement. In-depth knowledge of the mandibular structure is required to achieve these goals. This study used computed tomography (CT) to evaluate the mandibular cortical thickness and cancellous space according to age and sex. Methods: We enrolled 230 consecutive patients, aged 20 to 50 years, who underwent CT scanning. The cortex and cancellous space centered around the inferior alveolar nerve (IAN) canal were measured at two specific locations: the lingula and second molar region. Statistical analysis of differences according to increasing age and sex was performed. Results: The t-test revealed that the cancellous space and cortical thickness differed significantly with respect to the threshold of 35 years of age. Both cortical thickness and cancellous space in the molar region were negatively correlated with age. Meanwhile, both cortical thickness and cancellous space in the lingula region showed a positive correlation with age. With respect to sex, significant differences in the cancellous space at the molar region and the cortical thickness at the lingula were observed. However, no further statistically significant differences were observed in other variables with respect to sex. The sum of each measurement on the mandibular body reflected the safe distance from the surface of the outer cortex to the IAN canal. The safe distances also showed statistically significant differences between those above and below 35 years of age. Conclusion: Knowledge of the anatomical structure of the mandible and of changes in bone structure is crucial to ensure optimal surgical outcomes and avoid damage to the IAN. CT examination is useful to identify changes in the bone structure, and these should be taken into account in the planning of surgery for older patients.
Byun, Hyunjong,Park, Jiyeon,Kim, Sun Chang,Ahn, Jung Hoon American Society for Biochemistry and Molecular Bi 2017 The Journal of biological chemistry Vol.292 No.48
<P>Efficient protein production for industrial and academic purposes often involves engineering microorganisms to produce and secrete target proteins into the culture. <I>Pseudomonas fluorescens</I> has a TliDEF ATP-binding cassette transporter, a type I secretion system, which recognizes C-terminal LARD3 signal sequence of thermostable lipase TliA. Many proteins are secreted by TliDEF <I>in vivo</I> when recombined with LARD3, but there are still others that cannot be secreted by TliDEF even when LARD3 is attached. However, the factors that determine whether or not a recombinant protein can be secreted through TliDEF are still unknown. Here, we recombined LARD3 with several proteins and examined their secretion through TliDEF. We found that the proteins secreted via LARD3 are highly negatively charged with highly-acidic isoelectric points (pI) lower than 5.5. Attaching oligo-aspartate to lower the pI of negatively-charged recombinant proteins improved their secretion, and attaching oligo-arginine to negatively-charged proteins blocked their secretion by LARD3. In addition, negatively supercharged green fluorescent protein (GFP) showed improved secretion, whereas positively supercharged GFP did not secrete. These results disclosed that proteins' acidic pI and net negative charge are major factors that determine their secretion through TliDEF. Homology modeling for TliDEF revealed that TliD dimer forms evolutionarily-conserved positively-charged clusters in its pore and substrate entrance site, which also partially explains the pI dependence of the TliDEF-dependent secretions. In conclusion, lowering the isoelectric point improved LARD3-mediated protein secretion, both widening the range of protein targets for efficient production via secretion and signifying an important aspect of ABC transporter–mediated secretions.</P>
Son, Hyunjong,Lee, Ji Ha,Kim, Yang-Rae,Lee, In Su,Han, Sanyang,Liu, Xiaogang,Jaworski, Justyn,Jung, Jong Hwa The Royal Society of Chemistry 2012 The Analyst Vol.137 No.17
<P>A new BODIPY dye conjugate has demonstrated selective quenching by mercury over other metal ions. Coupling of this probe to Au–Fe<SUB>3</SUB>O<SUB>4</SUB> nanoparticles as well as platinum electrodes offered sensitive systems for suspension and surface based sensing, respectively.</P> <P>Graphic Abstract</P><P>Immobilization of the probe onto multiple platforms as a “turn-off” mode sensor revealed the robust nature of the BODIPY derivative in addition to showing its selectivity for Hg<SUP>2+</SUP> detection over a range of other competing metal ions. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2an35704f'> </P>