담배 니코틴에 의한 사람 태아 성상세포에서 종양괴사인자(TNF-α)의 발현 억제작용

손일홍,이성익,양현덕,한선정,석승한,이재규,김재현,박주영,문형인,이성수,Son, Il-Hong,Lee, Sung-Ik,Yang, Hyun-Duk,Han, Sun-Jung,Suk, Seung-Han,Lee, Jai-Kyoo,Kim, Jae-Hyun,Park, Joo-Young,Moon, Hyung-In,Lee, Sung-Soo 대한화학회 2007 대한화학회지 Vol.51 No.3

니코틴은 사람 대식세포에서 interleukin 2 (IL-2)와 종양괴사인자 (tumor necrosis factor-alpha; TNF-α) 가 생성되는 것을 억제하는데, 이러한 억제작용은 cytokine 유전자 발현 중 전사단계에서 전사인자의 활성을 억제함으로써 일어난다. 이러한 니코틴의 면역반응 억제작용은 아프타성궤양 및 궤양성대장염, 알레르기성폐 포염, 건초열 등에서도 보고되고 있다. 만일 중추신경계에서도 위와 같은 니코틴의 면역억제 작용이 일어난 다면 다발성경화증과 같은 면역반응 매개질환의 치료에 새로운 전기가 마련될 수 있을 것이다. 본 연구에서 는 중추신경계의 여러 면역반응 매개질환의 병태생리에 대한 이해를 넓히고자, 이미 알려진 니코틴의 cytokine 생성억제가 사람 중추신경계의 성상세포에서도 일어남을 확인하고 그 억제기전을 밝히고자 하였다. 이를 위 하여 사람 태아 성상세포에 다양한 농도의 니코틴과 IL-1β를 처리한 다음 TNF-α mRNA의 발현 정도와 NF- κB의 활성을 비교, 분석하여 다음과 같은 결과를 얻었다. 1. 사람 태아 성상세포를 0.1-20 μg/ml의 니코틴으로 처리해 본 결과 10 μg/ml 이상의 농도에서 세포독성능이 나타나기 시작하였다. 2. 사람 태아 성상세포에 IL- 1β를 처리하면 2시간만에 TNF-α mRNA가 최대로 발현되었으며 그 이후로는 점진적으로 감소하였다. 3. 사 람 태아 성상세포를 1 및 0.1 μg/ml의 니코틴으로 전처리한 후 IL-1β로 자극한 군에서는 IL-1β 단독 처리군에 비해 TNF-α mRNA의 발현이 감소하는 양상을 보였다. 1 μg/ml의 니코틴을 처리한 경우에는 8시간 이후부터 TNF-α mRNA의 발현이 현저하게 감소하여 12시간에 최대로 감소하였다. 또한 0.1 μg/ml의 니코틴을 처리한 군에서는 24시간에 가장 현저하게 감소하였다. 4. 성상세포에 IL-1β로 처리한 군에서는 강력한 NF-κB의 활성 을 확인할 수 있었으며, 니코틴을 전처리하고 IL-1β 자극한 군에서는 NF-B의 활성이 감소하였다. 결론적으로 일정농도 이상의 니코틴은 세포독성효과를 나타내나 적정한 농도와 시간 경과후 니코틴은 사람 태아 성상세포에서 IL-1β에 의해 유도되는 TNF-α의 발현 감소를 유도하며, 이는 NF-κB의 활성을 감소시킴으로써 나타난다고 생각된다. The Tumor necrosis factor-α, (TNF-α), is involved in the pathogenesis of multiple sclerosis and contributes to the degeneration of oligodendrocytes as well as neurons. Nicotine has been found to have immunosuppressive and inflammation-suppressing effects. Astrocytes, the major glial cells in the CNS, are capable of producing TNF-α at both the mRNA and protein levels in response to interleukin-1 (IL-1) or TNF-α. Nicotine has been shown to influence glial cell functions. To order to explore the role of astrocytes in the production of TNF-α, astrocytes were pretreated with nicotine and are stimulated with IL-1β to determine their effects on TNF-α production. The results are as follows. Cytotoxic effects of nicotine on human fetal astrocytes were noted above 10 μg/ml of nicotine. The effect of IL-1β on TNF-α mRNA expression in primary cultured human fetal astrocytes was maximal at 2 h after IL- 1β(100 pg/ml) treatment. Human fetal astrocytes were pretreated with 0.1, 1, and 10 μg/ml of nicotine and then stimulated with IL-1β (100 pg/ml) for 2 h. The inhibitory effect of nicotine on expressions of TNF-α mRNA in human fetal astrocytes with pretreated 0.1 μg/ml of nicotine is first noted at 8 hr, and the inhibitory effect is maximal at 12 h. The inhibitory effect at 1 μg/ml of nicotine is inhibited maximal at 24 h. Nicotine at 0.1, 1 and 10 μg/ml concentrations significantly inhibits IL-1β-induced NF-κB activation. Collectively, this study indicates that nicotine might inhibit the expression of TNF-α in activated human fetal astrocytes.

The Potential ‘O-GlcNAc-P’om’

Il Soo Moon(문일수),HyunSook Lee(이현숙),Hyung Jong Lee(이형종) 한국생명과학회 2013 생명과학회지 Vol.23 No.2

O-GlcNAc 화(O-GlcNAcylation)는 단백질의 serine이나 threonine에 N-acetylglucosamine (GlcNAc) 분자가 결합하는 것으로, 기존의 당단백질과 달리 세포질 및 핵단백질 모두에 일어난다. 또한 수정의 속도가 빠르고 가역적으로 일어남이 인산화 수식과 유사하다. 그러나 수많은 인산화효소와 탈인산화효소가 관여하는 것과 달리 O-GlcNAc 수식은 O-GlcNAc transferase (OGT)와 O-GlcNAcase (OGA) 단 두 개의 효소에 의하여 이루어진다. 이러한 단순한 조절기전은 세포가 내외환경에 즉시 적응할 수 있도록 진화한 것으로 해석된다. 즉, O-GlcNAc 수식은 특정한 단백질 하나 하나의 활성을 켜거나 끄는 것이 아니라, 세포의 신호전달과정의 효율을 전반적으로 조절하는 ‘가변저항기(rheostat)’ 역할을 한다. O-GlcNAc 수식은 흔히 같은 아미노산 혹은 그 주변의 아미노산이 인산화되는 것을 수반하는데, 이는 인산화와 함께 서로 조화를 이루어 세포활성을 조절하는 것으로 해석된다. 최근 O-GlcNAc이 더 나아가 O-GlcNAc-P로 인산화될 가능성이 제시되고 있는 바, 본 총설에서는 이의 가능성을 이론적으로 설명하고, 실제 실험결과를 소개한다. The addition and removal of N-acetylglucosamine (GlcNAc) molecules on serine or threonine residues of a protein is called O-GlcNAcylation. This post-translational modification occurs on both cytoplasmic and nuclear protein, and is fast and reversible as comparable to phosphorylation. In contrast to the phospho-signaling cycles, this emerging moon-lightening signaling is cycled by only two enzymes, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). The simple machinery is a good evolutionary adaptation of a cell for quick accommodation to continuously fluctuating intra- and extracellular microenvironments. Rather than “switching” on or off a specific proteins ? this would be done by phosphorylation where numerous specific kinases and phosphatases are involved - O-GlcNAcylation would play a “rheostat” which would be much more delicately increase or decrease the efficacy of signal transductions in response to cellular nutrient and stress conditions. Interestingly, recent evidence indicates that O-GlcNAc is further modified by phosphorylation. The O-GlcNAc-P will upgrade the modulation efficiency of cellular processes to continuous ‘analogue’ level. So far, only one protein AP180 was reported to have O-GlcNAc-P on Thr310. But, proteomic data from our laboratory indicate that there are multiple O-GlcNAc-P proteins, constituting “O-GlcNAc-P’om”. This will focus on the possibility of existence of “O-GlcNAc-P’om”.

여성 복압성요실금에 대한 Tension-free Vaginal Tape(TVT) 수술 성적

문찬,강윤일,김경영,문형윤,노준,김철성 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

Background: We wanted to evaluated the long term results of the tension-free vaginal tape (TVT) Procedure for treating strss urinary incontinence in women. Method: During Octover 1, 1998 to December 31, 2005, we evaluated the 52 women who underwent the TVT procedure for stress urinary incontinence at least 1 year following surgery. Preoperatively, Patients were evaluated with history taking, Physical examinations and urodynamic study, Operation and hospitalization data were evaluated with duration of procedure, hospital days, foley catheter removal days and use of analgesics, Postoperative evaluations included the uroflowmetry, residual urine volume, surgical outcomes, complications and patients' satisfaction with the procedure. Results: The follow-up period was a mean of 16 months. Preoperative I-QoL total score was 58.0. Comparision the result between preoperative and postoperative Q max was significantly shoter in the postoperative result. TVT procedure remained cure in 96.2% (cured: 82.7%, improved: 13.5%) and successful in 96.1%. Also, 49(94.2%) Patients would like to recommended the TVT procedure to others. There was no serious or long-term complications, Conclusions: The TVT procedure appear to be effective and safe for the surgical treatment of female stress urinary incontinence and showed a good long-term cure rate.

연령에 따른 한국인의 음향지표 변화와 특성

김형태,조승호,윤성문,선동일,김민식 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.1

Angiotensin Ⅱ로 유도된 혈관신생에 대한 日川芎의 억제효과

文成一,金亨煥,趙貞姬,安德均,崔湖榮 대한본초학회 2002 大韓本草學會誌 Vol.17 No.2

Objective : To estimate the angiogenic effect of water extract of Cnidii Rhizoma (ECR), we employed the rat sponge model for angiogenesis. Methods : It was based on subcutaneous implantation of sterile polyester sponges in the SD rat and we have characterized the dye method that incorporates 5% carmine red and 1% cresyl violet in 10% gelatin. vascularity index (V.I.) expressed as ㎍ dye/mg tissue. Subcutaneous implantation of sterile polyester sponges in SD rats enhanced the neovascularization over 14 days, as determined by measurements of relative sponge blood flow by a vascularity technique. The angiogenic response was verified by histological evaluation of vascularized sponges. Results : Daily oral administration of ECR (100 mg/kg for 14 days) produce no apparent effect on angiogenesis. The vasoconstrictor peptide, angiotensin Ⅱ (AⅡ), in daily doses of 100 nmol, caused an intense neovascularization but lower doses of AⅡ produced no apparent effect (1 nmol and 10 nmol). Co-administration of 100 nmol AⅡ and 100 mg/kg ECR p.o. depressed 100 nmol AⅡ-induced angiogenic effect. Conclusion : We have shown that p.o. daministration of 100 nmol AⅡ accelerated angiogenesis. Meanwhile, co-administration of 100 nmol AⅡ and 100 mg/kg ECR highly suppressed angiogenic property.

Si₃N₄/SiO₂/Si₃N₄열차단막을 이용한 유속센서 제작

이형주,김도훈,김진섭,박세일,최용문,권대혁,이정희 경북대학교 센서기술연구소 2002 센서技術學術大會論文集 Vol.13 No.1

방광에 발생한 염증성 근섬유아세포종

강윤일,문형윤,노준,김철성 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Inflammatory myofibroblastic tumor of urinary bladder is a rare benign entity that clinically and radiologically simulates a malignant tumor. This benign lesion must be differentiated histoloeically from several malignant lesion of the bladder. Complete surgical excision, either by transurethral resection or partial cystectomy, appears to be curative. We report a case of inflammatory myofibroblastic tumor of the urinary bladder in a 25-year-old female.

小兒期 皮膚筋炎 2例

李相一,尹龍洙,文炯魯,洪彰義 최신의학사 1980 最新醫學 Vol.23 No.6

Roll-Mill을 이용한 재생 발포스티렌과 고무 Blend의 기계적 물성

윤형구,박문수,허양일 全南大學校 觸媒硏究所 1998 觸媒硏究 論文集 Vol.20 No.-

In order to recyling expended polystyrene (EPS) wastes, volume-reduced EPS and rubber (NR, SBR) blends were prepared by melting-blending in a injection molder and investigated its mechanical properties such as impact strength and tensile strength. The volume-reduction process of EPS carried out by hot-pressed at 150℃ for 10 min using a roll-mill (called RPS) and compared with a hot-wind contacting method (TPS). Impact strength of RPS/rubber blend a increase of 2-5 times as compared with TPS/rubber blends. When the contents of rubber in RPS/SBR blends were around 15 wt%, maximum tensile modulus was obtained.

Inhibition of IL-32 Signaling by Bamboo Salt Diminishes the TSLP Production and Macrophage Differentiation in Human Monocyte Cell Line, THP-1 Cells

Hyun-Ja Jeong,Sun-Young Nam,Hyun-A Oh,Na-Ra Han,Young-Sick Kim,Il-Sook Choi,Phil-Dong Moon,Hyung Min Kim 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10