Escherichia coli(M15)을 이용한 serotonin N-acetyltransferase 유전자의 발현

김용만,정미영,윤경식,진병관,백행운,조용호,백형환 慶熙大學校 1997 論文集 Vol.26 No.-

AA-NAT cDNA was obtained by RT-PCR technique from total RNA of rat sacrified at night(02:00am). pCRNAT was cloned using the pCRII vector with insertion of AA-NAT cDNA(about 1.4 kb) at EcoRI site. For the expression of the gene, pQECNAT was subcloned, in which the coding region of AA-NAT was inserted into expression vector pQE30 at BamHI and HindIII sites. According to the experimental results, Escherichia coli strain M15, transformed by the expession vector pQECNAT, was selected as a host to express AA-NAT gene with the induction of isopropyl β-D-thiogalactoside(IPTG). Optimal condition for the expression of AA-NAT gene was achieve from the experimental results, showing that the expression of the protein was inducible much 19.800 ± 2,200 dpm per ml of culture volume in 4 hours at the concentration of 2 mM IPTG. Partial purification through the affinity column(Ni-NTA agarose) binding to the continuous 6 histidine residues of protein resulted in 5 times more increase in the specific activity of AA-NAT than that of the homogenate of bacterial pellet. These experimental results will provide basic data in further study for the enzymatic kinetics and antibody production of AA-NAT.

學校長의 指導性類型. 狀況的 要素. 學校組織 建康間의 關係硏究

金炯寬 高麗大學校 敎育問題硏究所 1988 敎育問題硏究 Vol.1 No.-

合成洗劑가 빨간집모기의 生態, 生理學的 特性에 미치는 影響

金正和,李炯來,朴垠澈,李容揆,崔觀善 충북대학교 농업과학기술연구소 1995 農業科學硏究 Vol.12 No.2

It is considered a general experience that migration from rural to urban areas will go on in any developing countries pursuing economic development through industralization. In Korea, migration is considered one of the serious socio- economic problems because it has been made excessively fast than any other countries' experiences. The socio- economic problems originates in migration can be classified in two ways ; the one is the overpopulated problems of urban areas and emptied villages problems of rural areas, and the other is the shartage agricultural labor farce problems symbolized by the increasing idle cultivated land. To relieve migration effectively, the developing policies should be converted from pursuing fast industralization and urbanization to balance growth between areas and industries. Moreover problem solving political measure should be converted from partial and corresponding approaches to total approaches containing economic, social and institutional side-view.

중년여성들의 숙련자와 비숙련자간 수영운동 후 신체조성 비교

김형묵,박관도 청주대학교 학술연구소 2004 淸大學術論集 Vol.4 No.-

The purpose of this study is to analyze 'how swimming' for twelve weeks affects the body development of middle-aged women. An investigation into the body development of thirteen women in a two-month period and a one-year period was performed. Each woman that participated in the swimming trials was asked to continually practice one hour in the morning three times a week before and after the investigation. The study arrived at the following conclusion. 1) The weight after swimming was found to have decreased significantly by 0.7kg in the case of the unskilled group and 0.6kg in the case of the expert group. 2) FAT(%) before and after investigation had similarly decreased by 2% in the case of the unskilled group and by 1.7% in the case of the expert group. FAT percentages(%) of the unskilled group were reduced by more than 0.3% when compared to the expert group. 3) FAT mass(kg) before and after the investigation had similarly decreased by 1.3kg in the case of the unskilled group and by 1.1kg in the case of the expert group. FAT mass(kg) of the unskilled group was reduced by more reduced by more than 0.2kg in comparison with the expert group. 4) The variation of lean body mass(kg) before and after the investigation had increased by 0.8kg in the case of the unskilled group and by 0.5kg in the case of the expert group. Lean body mass(kg) of the unskilled group increased by more than 0.3kg in comparison with the expert group. 5) Total body water before and after the unskilled group and expert group didn't change. In summation, swimming for one hour three times a week was found to reduce the FAT(%) and FAT mass(kg) of middle-aged women.

3원계 화합물 반도체의 에너지 밴드 구조와 전기적 특성 연구

김철회,김형관,한백형 漢陽大學校 基礎科學硏究所 1994 基礎科學論文集 Vol.13 No.-

Empirical pseudopotential 방법을 이용하여 아연 화합물 반도체의 에너지 밴드 구조를 구하였다. ??의 에너지 간격은 InSb의 조성비에 따라 ??=0.606χ²-0.727χ+0.361를 얻었고 전자이동도는 χ=0.6, 합금산란 포텐셜이 0.82eV, 온도가 77。K, 불순물 농도가 ??일때 6.7 × 10⁴cm/V-s을 얻었다. Energy band structure of zinc-blende semiconductors is calculated by the empirical pseudopotential method using the adjustable parameter. Energy gap of ?? with InSb mole fraction is ??=0.606χ²-0.727χ+0.361 and its electron mobility is found to be a high value of 6.7 × 10⁴cm/V-s with mole fraction x=0.6, alloy scattering potential ΔU=0.82eV, Temperature T=77。K, and impurity concentration ??.

화상처리를 이용한 지능형 가공시스쳄의 설계 및 구현

김관형,김수정,김영탁,문희근,이상배 한국해양대학교 산업기술연구소 2003 연구논문집 Vol.20 No.-

본 논문은 자동화 시스템에 적용 가능한 지능 제어 시스템에 관한 연구이다. 따라서 본 연구에서는 지능제어 이론과 영상처리기술들을 이용하여 지능형 복합제어시스템을 능동적으로 제어하는 것을 목적으로 한다. 지능형 복합제어시스템의 성능 평가와 제어 전략은 각각 신경회로망과 퍼지이론을 이용하였고, 영상 처리기법을 이용하여 물체의 특정 위치를 추출하였다. 실제 시스템에 적용하여 얻은 실험 결과를 통하여 본 논문에서 제안한 방버의 유효성을 제시하고자 한다. This paper is research into intelligent control system that is able to apply at automatic system. So, in this paper we are in control of intelligent compound control system actively for the use of intelligent control theory and image processing. Performance evaluation and control strategy of intelligent compound contro system were used to neural network and fuzzy theory, we proposed detection of particular position using image processing we propose availability of proposed method based experiment result that apply to real system in this paper.

韓國의 工業所有權 制度에 關한 硏究

金寬衡 건국대학교 행정대학원 1989 建國大學校 行政大學院 硏究論叢 Vol.17 No.-

지능기법을 이용한 가공시스템의 설계 및 모니터링에 관한 연구

金永卓,文熙根,金秀貞,金寬衡,李尙培 한국해양대학교 산업기술연구소 2003 연구논문집 Vol.20 No.-

본 논문은 지능 기법과 영상처리기법을 이용하여 특정 대상물의 절단위치를 검색하는 방법을 제시한 것으로서, 최근에는 기존의 아날로그 센서를 대신한 CCD(Charge-Couple Decive)카메라로 얻어진 화상 데이타(data)를 이용하여 물체의 정보를 추출하는 방법이 많이 응용되고 있다. 최근에 영상의 형태학, 신경망, 지식기반의 영상 해석 시스템에 대한 관심이 높아지면서, 거의 모든 분야에 화상처리기법들이 사용되어지고 있다. 따라서 본 논문에서는 실시간 그레이 레벨의 입력 화상에 대하여 영상처리기법을 이용하여 특정 물체의 특정위치검출 방법을 제안하고, 실제의 시스템에 적용한 예를 제시하려고 한다. In this paper proposed detecting specific position of the object using intelligent method and image processing. Recently instead of classifying the object by shade using existing analog sensor, app.y a theory to detect of the object information by using image data from CCD camera. It takes interest in the morphology of image, neural networks and image analysis system on knowledge base. and now days, image processing method was used in the most part of field. So, In this paper proposed detecting method on specific position of specific object about real time gray level input image using image processing method and application case in real system.

CuInSe₂의 결정구조에 관한 연구

김형관,이건상,김옥경 漢陽大學校 基礎科學硏究所 1988 基礎科學論文集 Vol.7 No.-

Se를 화학당량비보다 10% 더 첨가하여 2단전기로를 사용하여 용융법에 의해 성장시킨 CuInSe₂의 결정화 여부를 x-ray diffractometer로 측정한 회절무늬와 계산된 회절무늬를 비교하여 결정하였다. 성장된 결정은 격자상수가 a=5.78Å과 c=11.59Å인 chalcopyrite 구조를 가지며 c/a=2.005로 신장 외곡을 하고 있다. Ⅵ족 원자의 위치를 결정하는 값 x는 x=0.2487이며, ??=2.5007Å, ??=2.5095Å으로 Se가 Cu쪽에 가깝게 위치하고 있다. 따라서 성장된 결정은 이온분극을 가진 것으로 해석된다. CuInSe₂ crystals are grown by directional freezing method using two-zone furnace. The crystal structure are investigated using x-ray diffractometer by powder method and compared with calculated data. The lattice constants are calculated a=5.78Å and c=11.59Å. The distance between atoms are ??=2.5007Å and ??=2.5095Å. It is found that Se atoms are positioned toward Cu atoms.

망막아세포종 세포주(Y-79)의 Arylalkylamine N-acetyltransferase 활성에 미치는 Dopamine의 영향

김석민,정미영,윤경식,진병관,백행운,백형환,조용호 慶熙大學校 1997 論文集 Vol.26 No.-

The arylalkylamine N-acetyltransferase(AA-NAT, EC 2.3.1.87) in vertebral pineal gland and retina is rate-limiting enzyme in the pathway of biosynthesis of melatonin. AA-NAT activities are remarkably increased during night with 10-150 folds. Neurohormone melatonin is thought to play roles in a broad range of physiological functions in human, primarily through effects on the biological clock in the suprachiasmatic nucleus of the hypothalamus, This rhythm reflects large changes in the activities of AA-NAT. So the important regulatory role of AA-NAT has made it central interest in research on melatonin biochemistry and neurochemical signal transduction. The neurotransmitter that regulates the AA-NAT activities is norepinephrine, which acts through a cyclic AMP mechanism. The effect of dopamine on AA-NAT in human retinoblastoma Y-79 cells was studied. the obtained results are as follows; AA-NAT activities in Y-79 cells were increased by cyclic AMP, and dopamine inhibited the AA-NAT activities stimulated by forskolin. The pattern of inhibition of dopamine on the AA-NAT activities in Y-79 cells was time and concentration dependent. The results suggest that dopamine participates in the regulation of the AA-NAT activities through the inhibitory mechanism in the human pineal and retinal tissues.