Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

저작근 공간에서 재발된 치성각화낭

임수연,허경회,이원진,최현배,최순철 대한구강악안면방사선학회 2008 Imaging Science in Dentistry Vol.38 No.2

The odontogenic keratocyst (OKC) is a developmental odontogenic cyst typically occurring in the jaws. Since the first description of OKC was published in 1956, the lesion has been of particular interest because of its specific histopathologic features, high recurrence rate, and aggressive behavior. Recurrences most commonly arise within bone at the site of the original cyst. However, as lining cells may find their way into surrounding tissues either from implantation during surgery or from cortical perforation recurrences may arise at a distance from the original cyst. Here, we report a rare case of recurrent OKC which was first developed in mandible and recurred within the masticatory space.

대전 3, 4 공단지역의 대기중 중금속의 농도

임종명,이진홍,윤미정,장미숙,남병현 忠南大學校 環境問題硏究所 1999 環境硏究 Vol.17 No.-

This research centered on seasonal analysis for the concentration of heavy metals in 5 sites within Taejon 3rd and 4th industrial Complexes. Total suspended particulates were collected on glass microfibre filters (Whatman EPM 2000) by high volume air samplers. About 20 metal elements including 13 toxic ones were analyzed by ICP-MS and ICP-AES after the pretreatment of mixed acid extraction. The following results were summarized from the research : 1) the concentrations of TSPs were 17.7∼219.6㎍/㎥ while the arithmetic mean concentration was 101.7㎍/㎥ ; 2) the arithmetic mean concentration of human carcinogen such as arsenic, hexavalent chromium and nickel subsulfide was 9.99, 6.68 and 6.13 ng/㎥ while that of probable human carcinogen such as beryllium, cadmium and lead was 0.12, 3.37, 97.65 ng/㎥, respectively.

인터넷을 활용한 진로정보탐색 프로그램이 대학생의 진로 자기효능감에 미치는 영향

임은미,김현진 전주대학교 사회과학종합연구소 2002 社會科學論叢 Vol.18 No.-

본 연구는 인터넷을 활용한 진로정보탐색 프로그램이 대학생들의 진로자기효능감에 미치는 영향을 검증하기 위한 것이다. 이에 대학생을 대상으로 하는 인터넷을 활용한 진로정보탐색 프로그램을 제작하였다. 이 프로그램은 김봉환 등(2001)이 제안한 모듈식 진로상담 프로그램의 개념을 임은미와 장선숙(2002)이 중등학생을 위해 적용한 것을 대학생의 상황에 맞게 수정하여 제작되었다. 프로그램 회기는 4회기로 구성되어 있으며, 그 내용은 프로그램 분위기 조성 및 온라인 직업흥미검사 실시(60분), 직업흥미검사 결과의 해석과 직업세계의 이해(60분), 정보사냥대회(60분), 직업정보탐색과 마무리로 이루어져 있다. 프로그램의 실시 전후에 <진로의사결정 자기효능감 검사>를 실시하고, 사전-사후검사 점수 차이를 분석하여 프로그램의 효과를 검증하였다. J대학교의 1학년 남녀학생 가운데 자발적인 신청자들을 대상으로 본 프로그램을 적용한 후 실험집단과 통제집단의 사후검사 점수 차이를 비교한 결과 프로그램 참가자들은 통제집단에 비하여 사후검사에서 의미있게 높은 점수를 얻었다. 이러한 경향은 진로 의사결정 전체 Q뿐 아니라 문제해결 하위영역을 제외한 목표설정, 계획수립, 자기평가, 직업정보탐색의 전 하위 영역에서도 동일하게 나타났다. 이러한 결과는 대학생을 대상으로 인터넷을 활용하여 단기에 실시하는 진로정보탐색 프로그램이 대학생의 진로의사결정 효능감을 높여줄 수 있음을 시사한다. The purpose of this study is to examine the effects of career information exploration program using internet on the undergraduates' career decision-making self-efficacy. For this purpose, career information exploration program using internet was developed. The main factors of this program are performing the free on-line interest test, explorating the career information web-sites, and getting career information through internet according to the participants' interests. The results showed that the experimental group got higher score than the control group in CDMSES(Career Decision-Making Self-Efficacy Inventory). But for the problem solving subcategory, this tendency was shown in all the sub factors of CDMSES, i.e. goal-setting, career planning, collecting information, and self -evaluation. The implications about education or counseling programs for the undergrauates were discussed.

장기 알콜투여가 생쥐의 간 및 신장, 위장 조직 손상에 미치는 영향

김진택,김동환,안상현 東國大學校韓醫學硏究所 1994 東國韓醫學硏究所論文集 Vol.3 No.-

Alcohol is a major risk factor for several diseases and excessive, long-term alcohol consumption are cause physical alteration-fatty liver, hepatitis, cirrhosis, breaking down, Wernicke-karasakoffs syndrome, weight loss, and poor immunity-in virtually all organ and tissue, This study was observed that liver, kindney, and stomach were altered in mouse by the effect of chronic alcohol administration. The mouse were sacrificed to obtain the tissue after mouse were orally injected with 25% ethanol 18㎖/kg/day for 120days. The tissue were stained by hematoxylin and eosun and then obseved by light microscope. The results of this study were as follows : 1. The congestion was appeared in liver after 120days alcohol administration 2. The destruction of glomerulus were increased and parietal cell of Bowman's capsule were swelled such as cuboidal cell after 12days alcohol administration. The congestion was appeared in alcohol administrated group. 3. The mucosa and gasstric pit were destructed and the ulceration was appeared in stomach after 120days administration. The parietal cells and chief cells were damaged. Above results were shown that the tissue were damaged by chronic alcohol administration.

ICP-AES를 이용한 대기분진 중 중금속 분석에 대한 정도관리

임종명,이현석,이진홍 忠南大學校 環境問題硏究所 2003 環境硏究 Vol.21 No.-

In this study, factors of quality control such as laboratory environment, capability of instrument/equipment, method of analysis, calibration, and standard solution were investigated to obtain quality assurance when analyzing airborne heavy metals (e.g., Pb, Cr, Cd, Cu, Mn, Ni, Fe) using ICP-AES. A procedure of quality assurance and evaluation method of uncertainty are also suggested by applying ISO Guide. The suggested process of quality assurance will be helpful for the national monitoring network to produce better data.

기기 중성자방사화분석법을 이용한 황사특성

임종명,이현석,이진홍 忠南大學校 環境問題硏究所 2002 環境硏究 Vol.20 No.-

Using INAA, which has been thought to be one of the best analytical techniques for airborne particulate matters, this study centered on the quantitative analysis of 32 metal elements within Asian Dust aerosols. Concentrations of REEs of crustal origin, such as Ce, Cs, Dy, La, Sc, and Sm were more than 8.5 times higher in Asian Dust aerosols. In addition, major crustal elements, such as Al, Ca, Co, Fe, K, Mg, Mn, and Ti showed more than 3 times higher concentrations in those aerosols, which made PM 10 and all metal concentrations be also about 3.0 and 4.8 times higher, respectively. However, concentrations of anthropogenic origin, such as As, Br, Ci, I, In, Sb, Se, and Zn were increased to be a little higher, which means no great effect of Asian Dust on these elements.

미국과 멕시코의 환경문제와 발전전략 : 비만형과 빈곤형의 연관

임현진,홍성태 서울대학교 사회과학연구소 1996 社會科學과政策硏究 Vol.18 No.3

Sr·페라이트의 자기특성에 관한 연구

임재근,서강수,문현욱,신용진 明知大學校 産業技術硏究所 1992 産業技術硏究所論文集 Vol.11 No.-

This thesis is the study on the magnetic properties of strontium (Sr) ferrite. Specimens of SrO·nFe₂O₃,basic composition, with n=5.9 which is nonstoichiomatric composition region were prepared by sintering at 1275°C for 1hr, whthin air surroundings. In order to increase coercivity, additives of 0.4wt%SiO₂ and 0.7wt%CaCO₃ were added into basic composition, and 0.5wt%∼2.0wt%Cr₂O₃ was added as second additives. In case of second additives 1.5wt%Cr₂O₃, it was obtained uniformed grain size and magnetic properties was increased. The sintering temperature was 1150°C, 1180°C, 1210°C, 1240°C and 1270°C respectivily and cooling was performed in furnance. From the research and experiments on the magnetic properties of strontium (Sr) ferrite based on the complex additive technique, it was obtained good magnetic properties of (BH)mas=0.72MGOe, at the sintering temperature of 1210°C, in the case of (basic composition)+(basic additive)+(1.5wt%Cr₂O₃)

효소적 방법으로 제조된 키토산 올리고당의 첨가에 따른 김치의 숙성 및 저장 기간 연장에 관한 연구

임현수,김진만,유은정,최명락 여수대학교 1998 論文集 Vol.13 No.2

효소로 처리된 키토산 올리고당의 첨가에 의한 김치의 숙성 기간 연장 효과를 알아보기 위하여 배추김치에는 0.2-0.4%, 2일째에 0.4%를 첨가하여 20℃에서 10일간 관찰한 결과 0.2% 첨가시 대조군에 비해 2일, 0.4% 첨가시는 4일, 2일째에 0.4%를 첨가한 경우는 8일 이후로 연장 효과가 2-4배 있었으며, 0.4% 이상에서는 미생물 생육자체가 억제되므로 풍미 및 유기산의 생성을 고려할 때 2일째 첨가된 0.4%가 젖산균의 수는 어느정도 유지하면서 stationary phage를 연장시킴으로서 더 좋은 결과를 나타냈다. 갓 김치의 경우 0.1%-0.5% 첨가시 대조군에 비해 1.2-1.4배의 연장 효과가 있어서 배추 김치에 비해서는 낮은 효과를 나타냈다. 따라서, 효소로 처리된 키토산 올리고당의 첨가에 의해 배추 김치는 2-4배의 연장 효과, 갓김치는 1.2-1.4배의 연장 효과를 나타내어 미생물 증식 억제에 의한 김치의 숙성 및 저장 기간 연장 효과를 기대할 수 있었다. To prolong the fermenting and preservation period of Kimchi, chitosanoligosaccharide was prepared by chitosanase. Various concentrations of chitosanoligosaccharide were added into Chinese cabbage Kimchi and Gat Kimchi. Chinese cabbage Kimchi was formulated with various chitosanoligosaccharide concentrations(0.2-0.4%) and fermented at 20℃. And Gat Kimchi was prepared with various chitosanoligosaccharide(0.1-0.5%) concentrations during 26 days at 4℃. In this results, the fermentation period was increased 2-2.5 and 1.2-1.4 folds more than control(0% of chitosanoligosaccharide) for Chinese cabbage Kimchi and Gat Kimchi, respectively. Conclusively, adding of chitosanoligosaccharide into Kimchi significantly prolonged the fermentation and preservation period.