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Comparison of Mechanical and Enzymatic Methods for the Isolation of Bovine Ovarian Follicles
Lim, Hyun-Joo,Kim, Dong-Hoon,Im, Gi-Sun,Lim, Jeong-Mook 韓國受精卵移植學會 2010 한국동물생명공학회지 Vol.25 No.2
The isolation of preantral follicles from the ovaries of bovine was performed under mechanical and enzymatic methods. A significant increase in the total number of follicles retrieved was detected when tissue chopper was used. Micro-dissection could supply good quality, larger sized follicles (400 to ) but with the lowest yield (). The isolated preantral and early antral follicles were cultured for 14 days. Follicles isolated by the mechanical method had a greater growth during a culture period than follicles collected enzymatically. Morphologically normal bovine oocytes from early antral follicles after 14 days culture were 59.6% after culture and after 24 h of maturation culture, 12.9% of in vitro-grown oocytes reached the second metaphase. In conclusion, this study showed that mechanical method can be used effectively to isolate intact preantral follicles from bovine ovaries.
Stability of dental, alveolar, and skeletal changes after miniscrew-assisted rapid palatal expansion
Hyun-Mook Lim,Young-Chel Park,Kee-Joon Lee,Kyung-Ho Kim,Yoon Jeong Choi 대한치과교정학회 2017 대한치과교정학회지 Vol.47 No.5
Objective: Miniscrew-assisted rapid palatal expansion (MARPE) is a means for expanding the basal bone without surgical intervention in young adults. Here, we assessed the differences in dental, alveolar, and skeletal measurements taken before (T0), immediately after (T1), and 1 year after (T2) MARPE. Methods: Twenty-four patients (mean age, 21.6 years) who had undergone MARPE and cone-beam computed tomography at T0, T1, and T2 were included. Changes in the following parameters were compared using paired t -tests: intercusp, interapex, alveolar, nasal floor, and nasal cavity widths; inclination of the first molar (M1) and its alveolus; and thickness and height of the alveolar bone. A linear mixed-effects model was used to determine variables that affected periodontal changes in the M1. Results: MARPE produced significant increases in most measurements during T0−T2, despite relapse of some measurements during T1−T2. The alveolar thickness decreased on the buccal side, but increased on the palatal side. The alveolar crest level at the first premolar moved apically. Changes in the thickness and height of the alveolar bone were affected by the corresponding initial values. Conclusions: MARPE can be used as an effective tool for correcting maxillomandibular transverse discrepancy, showing stable outcomes 1 year after expansion.
In Vitro Growth of Bovine Preantral Follicle under Different Culture Conditions
Lim, Hyun-Joo,Kim, Dong-Hoon,Im, Gi-Sun,Hwang, Seong-Soo,Baek, Kwang-Soo,Jeon, Byeong-Soon,Park, Sung-Jai,Kim, Hyeon-Shup,Lim, Jeong-Mook The Korean Society of Animal Reproduction 2009 Reproductive & developmental biology Vol.33 No.4
The objective of this study was to determine effects of different culture media. Preantral follicles were mechanically extracted from bovine ovaries and cultured for 16 days in tissue culture medium (TCM)-199, DMEM or alpha-minimal essential medium ($\alpha$-MEM) + 10% FBS + 0.1 mg/ml sodium pyruvate + 100 mIU/ml FSH. The collected primary follicles from ovary were higher than the primary and secondary follicles. The survival rates of the follicles in TCM-199 were significantly higher (p<0.05) than those in DMEM and $\alpha$-MEM. The diameter of the follicles progressively increased during 12 days of culture. The maximum size ($139.1{\pm}5.4\;{\mu}m$) reached on Day 12 of the in vitro culture and decreased on Day 16. These results suggest that in a culture of bovine preantral follicles, TCM-199 is an optimal medium and a longer-term culture of preantral follicles (>12 days) may be needed to form antra.