RAPD와 핵 리보솜 DNA ITS2 염기서열에 의한 한국산 전나무속 식물의 유전적 변이 및 계통유전학적 분석

정용환,한상현,오유성,고미희,고석찬,김문홍,오문유 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2

We analyzed the genetic variation and phylogenetic relationship among fourteen taxa of GenusAbies distributed in Korea using random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) 2 sequences of the nuclear ribosomal DNA (rDNA). RAPDs were used for identification of selected taxa by amplification using single 10-mer primers of arbitrary sequence. Fourteen taxa were clearly classified with 10 arbitrary primers, which generated 102 useful polymorphic bands that were evaluated according to the presence or absence of the fragments of equal size. The genetic distance between A. koreana for. chlorocarpa and A. koreana for. rubrocarpa was 0.127, showing the closest among any other pairs. By comparing the base sequences in the ITS2 region of the nuclear rDNA, genetic relationship was assesed among selected taxa. It showed twenty six sites of point mutation (base substitution) in 243 base pairs (bp) of ITS2 region. The G+C contents of ITS2 region were ranged from 58.3% to 61.7%. The value of nucleotide divergence among selected taxa was ranged from 0.4% to 9.8%. These results showed that RAPD and ITS2 sequence analysis was a useful tool for elucidating phylogenetic relationship and selection of specific marker in Abies species.

자의퇴원환자 특성 및 요인에 관한 연구

김광환,서순원,이현실,조영채,박미경,정현경 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

This research aims to attract patients of AMA(Against Medical Advice). The patients are neglected because they are a few, but the patients are very important for hospital income. So, this study analysis patients of AMA. The analysis items are 1) a course of hospital, 2) health delivery system, 3) whether or not consultant, operation and transfer, 4) principle diagnosis, 5) specialty fields, 6) length of stay, so on. The results show that AMA of male, old, and emergency case is high. In principle diagnosis, AMA of cerebral infaction and fracture and liver disease is high. Therefore it's meaningful research for hospital management.

유도 '되치기 본'의 개선 방안에 관한 연구

김의환,김도준,김규수,김관현,김종달,최종삼,조용철,박순진,윤익선,안병근,정 훈,김미정,한성철 龍仁大學校 武道硏究所 1999 武道硏究所誌 Vol.10 No.1

The purpose of this study was to reform practically a Korean Judo's Doechigi-Bon(Forms of Counterattack, judo's Gaeshi no Kata, Judo's Uradori no Kata, Judo's Gonosen no Kata) that was established in 1955 Korea, according to changing of techniques by Judo's modernization, in order to have Judo's carefulness and systematic diffusion. Reform procedure of Judo's Doechigi-Bon was 1st stage, Questionnaire survey 303 judokas, 2nd stage, Technical seminar by judo experts(12 judo professor) 4 times, 3rd stage, wording report for reform, 4rd stage, Discussion and judgement of Teaching and Judgement commission of Korean judo Association(KJA), 5th stage, Public hearing for reform in KJA, 6th stage, Report and decision of board of directors in KJA, 7th stage, public publication of Judo News(No.53) in KJA. Basic principle of reform of Judo's Doechigi-Bon were as table 1. Table 1. Basic principle of reform of Judo's Doechigi-Bon ------------------------------------------------------------------------------- Items Reformed Key Points of Judo's Doechigi-Bon ------------------------------------------------------------------------------- 1. Structure 1) Grand classification : Classified by 3 parts(1,2,3Gyo) 2)Medium classification : Te waza, Goshi waza, Ashi waza devided per each part(Gyo) 3)Sub-classification : Classified five techniques per each part(Gyo) 2. Contents 1) Selected established techniques as possible 2) Considered rationalty and overlapping of counterattack techniques 3. Decision of Conterattack techniques 1) Refered to results of Basic Questionnaire survey 2) Priority to decisions of Judo expert technical seminar -------------------------------------------------------------------------------- Reformed Korean Judo's Doechigi-Bon(Forms of Counterattack techniques-KJA, 1999) are as follows: 1. Gyo; ① Uki otoshi -> Uchi mata ② Harai goshi -> Harai goshi gaeshi ③ O soto gari -> O soto gake ④ Ko uchi gari -> Sasae tsurikomi ashi ⑤ O uchi gari -> Ko soto gari 2. Gyo; ① Ippon seoi nage -> Okuri eri jime ② Tsuri domi goshi -> Uki waza ③ Okuri ashi harai -> Okuri ashi harai ④ Ko soto gari -> Tai otoshi ⑤ Hiza guruma -> Hiza guruma 3. Gyo; ① Kata guruma -> Sumi gaeshi ② Tai otoshi -> Ko soto gari ③ Hane goshi -> Harai tsurikomi ashi ④ Uchi mata -> Tai otoshi ⑤ Tomoe nage -> O uchi gari

數 種 韓藥의 아세틸콜린에스터레이즈 酵素活性에 대한 효과

최재환,김미영,김현미,최훈,전훈,김대근,임종필,임강현 대한본초학회 2002 大韓本草學會誌 Vol.17 No.1

아세틸콜린에스터레이즈(AChE)는 신경조직에 분포하는 효소로 신경전달물질 중 아세틸콜린(ACh)을 분해하는 효소로 시냅스 신호전달체계에 있어 중요한 역할을 하는 효소이다. 현재 알츠하이머병과 치매치료에 사용하는 서양약물은 AChE의 활성을 저해하는 작용기전을 가진다. 목적 : 전통적으로 기억력 감퇴, 치매 등 치료에 사용되어 온 한약의 AChE에 대한 작용을 밝히기 위해 본 실험이 구상되었다. 방법 : 생쥐 대뇌신경세포의 균질 혼합액으로부터 효소의 활성을 Ellman 방법을 개선한 엘리자(ELISA)법을 이용하여 측정하였다. 결과 : 실험결과 天花? 의 6종이 2.5㎎/㎖의 농도에서 우수한 효소활성저해력을 보였고, 50% 효소활성 저해농도(ID_50)를 구한 결과, 天花?의 ID_50은 1.5㎎/㎖이었고, ??? 1.2 ㎎/㎖, ?? 2.0 ㎎/㎖, ??草 1.2 ㎎/㎖, ??? 1.0 ㎎/㎖, ??生 1.7㎎/㎖, 및 大?皮 2.0 ㎎/㎖를 나타내어 忍冬?의 저해력이 가장 우수하였다. 결론 : 치매 및 기억력감퇴 증상을 치료하는 한약의 작용기전 중 AChE 효소활성억제효과는 忍冬? 의 6종에서 나타났으며, 이는 전통적인 한방치료에 과학적인 기초자료를 제공할 것으로 생각한다.

고대 韓民族의 생물 이용과 음양오행론 : BC 8,000년부터 AD 100년경까지 from BC 8000 to AD 100

金玄,申桓浩,宋美長 한국정신과학학회 2001 韓國精神科學學會誌 Vol.5 No.2

Investigation fields of tins study has mainly focused on diversity of bio-organisms and principle of bio-organism utilization practiced by the inhabitants of Far Eastern Asia from BC 8000 to AD 100. The investigation shows that Korean ancestors before 6000 years utilized various bio-organisms similar to modem Koreans. We also learned from the study that utilization of various bio-organism induced anginal principle In their applications. It was confirmed that applications of this principle were practically used by Korean ancestors after BC 4000. Upon review carried out on the food, clothing and shelter with bio-organism application at that time, we could find out that principle of bio-organism utilization was based on theory of the cosmic dual forces and five elements.

2000년 제27회 시드니 올림픽 유도경기 대비: 세계 여자 우수선수들의 개인별 국제경기 기술 특성에 관한 연구

김의환,박순진,김관현,김도준,안병근,정훈,김미정 용인대학교 무도연구소 2000 武道硏究所誌 Vol.11 No.1

The purpose of this study was to analyze the competition techniques traits and of international contest levels each category individual scoring - losing techniques of world elite judokas who. were awarded in the international judo tournaments (I,J.T.) (part 1: ∼O.G'%, part 2 :from O.G'% to July ,2000), and who are expected to participate in the 27th Olympic Games(O.G.) Sydney 2000 to prepare the 27th O.G, which will be held from 16. to 22 September, 2000 at Exhibition Center, Darling Harbour, in Sydney, Australia. The records of the results and contents of competition were obtained out of 97 for women I.J,T., world elite judokas. To decide individual contest levels, groups were devided into 3(A,B,C)groups and points were graded by 3 ways. The I.J.T. that were devided into 3 groups are shown in the table 1.

말단 비대증의 임상적 특성

박은미,안용환,김현정,김병수,임종주,김하영,박병현,조정구 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

고조선 이후 한민족의 생물 이용과 그 변화 양상 : AD 100년경부터 현대까지 from AD 100 to modern times

金玄,申桓浩,宋美長 한국정신과학학회 2001 韓國精神科學學會誌 Vol.5 No.2

Although principle of bio-organism utilization was established from the Go-chosun, the period of ethnobiological bio-organism utilization started after the Go-chosun which was actively utilized bio-organisms according to principle. The period of ethnobiological bio-organism utilization is divided in to duration of application, curtailment and loss Consequently, the Korean principle and method of bio-organism utilization lost progressively. It must be actively used through reconsideration of modem scientific methods.

신라대학교 주변 백양산의 동물상

노치권(외 6명) 신라대학교 자연과학연구소 2012 自然科學論文集 Vol.22 No.-

To study fauna of Silla University located in Mt. Baek-yang, we conducted six surveys from 29 May 2011 to 28 April 2012. We found five phyla 41 orders 197 species of animals inhabiting in this area: four phyla 27 orders 163 species of invertebrates and in vertebrates one order six species of fishes, three orders nine species of amphibians and reptiles, six orders 14 species of aves, and four orders five species of mammals. Of these species, some Korean endemic species were included: a fish of Rhodeus uyekii in a pond near main gate of this university, a frog of Rana coreana in a creek near a dormitory, and a rabbit of Lepus coreanus and a water deer of Hydropotes inermis in a grass region near a main stadium. A kind of hawk, Falco tinnunculus as a natural monument (animal) of no. 323-8, was observed on a building of medical and life science. A blue frog of Hyla japonica as a variation was found near a spring resort. Therefore, this area is considered an ecologically important area having a high animal diversity.

스토커형 저공해 소각로 설계를 위한 비반응 유동해석에 관한 연구

전영남,오현명,김미환 조선대학교 환경연구소 2000 環境硏究 Vol.16 No.1

The numerical simulation of non-reactive turbulent model and cold flow experiment were carried out to propose the incinerator type for the domestic refuses and to investigate the design factor and operating conditions. The "standard stoker incinerator" proposed in this study was modified from central flow type with carrying out by material and numerical simulations repeatedly. The stoker has the characteristics of good mixing between refuse and hot combustion gas in primary combustion chamber and between unburned gas inflowing and secondary air jet in secondary chamber. Also there was no recirculation zone in secondary chamber so that mixing time was increased with higher residence time. Therefore this incinerator could be possible for domestic refuse to burn up with the low emission and high efficiency combustion. Parametric screening studies were achieved in standard stoker incinerator. The parameters are stoker type, secondary air inflow type, secondary air injection angles, secondary injection air flow rate and primary air inflow rate. The results are as follows: 1. In the same conditions with standard stoker incinerator, flow pattern was not almost changed by changing inclined stoker type. 2. The injection potion and angle of secondary air had an effect on flow field in secondary combustion chamber. 3. Flow pattern was not almost changed with the variation of the flow rate of primary and secondary air injection. The results of parametric studies said that the main factors for the design are the incinerator configuration of secondary combustion chamber and/or the position and angle of secondary air injection.