게스트하우스 물리적환경이 고객만족 및 재방문의도에 미치는 영향 : 고객가치의 조절효과

강혜숙(Kang, Hyesook),문정인(Moon, Jungin) 대한관광경영학회 2018 觀光硏究 Vol.33 No.5

본 연구는 강릉지역 게스트하우스 물리적환경이 고객만족 및 재방문의도에 미치는 영향을 살펴보고, 게스트하우스 물리적환경이 고객만족 및 재방문의도에 미치는 영향관계에서, 고객 가치가 조절효과가 유효한지를 제시하는 데에 그 목적이 있다. 설문지 조사기간은 2018년 3 월 25일부터 6월 25일까지(약 3달)간에 걸쳐 설문지를 배포하였고, 그 중에서 회수한 설문지 257부를 실증분석에 이용하였다. 분석결과는 다음과 같다. 첫째, 게스트하우스 물리적환경 3 개 요인(물리적환경, 편의성 및 청결성, 주변환경특성) 중에서 물리적환경 요인과 편의성 및청결성 요인이 고객만족에 유의한 영향을 미치는 것으로 나타났다. 둘째, 게스트하우스 물리 적환경요인과 편의성 및 청결성 요인이 재방문의도에 유의한 영향을 미치는 것으로 나타났 다. 하지만 주변환경특성 요인은 재방문의도에 부의 영향을 미치는 것으로 나타났다. 셋째, 게스트하우스 물리적환경 요인과 고객만족의 영향관계에서, 조절변수로서 고객가치는 통계적 으로 유의하지 않은 것으로 나타났다. 넷째, 게스트하우스 물리적환경 요인과 재방문의도의 영향관계에서, 조절변수로서 고객가치는 통계적으로 유의한 것으로 나타났다. The purpose of this study is to examine the effect of physical environment on customer satisfaction and revisit intention in guesthouses in Gangneung regions, and so present whether the moderating effect of customer value is effective in the effect of physical environment on customer satisfaction and revisit intention in guesthouses. The period of questionnaire survey in this study was from March 25 to June 25, 2018(about three months), during which its questionnaires had been distributed. Of them, this study collected 257 copies of the questionnaire, which were used for its empirical analysis. The analysis results of this study are as follows. First, of 3 physical environment factors in guesthouses, physical environment factor, convenience factor, and cleanliness factor had significant effects on customer satisfaction. Second, physical environment factor, convenience factor, and cleanliness factor in guesthouses had significant effects on revisit intention. However, ambient environment characteristic factor had negative effects on revisit intention. Third, customer value as a moderating variable was not statistically significant in the relationships between physical environment and customer satisfaction in guesthouses. Fourth, customer value as a moderating variable was statistically significant in the relationships between physical environment and revisit intention in guesthouses.

漢字의 一人稱代名詞 本義 分析

강혜숙(Kang, Hyesook) 고려대학교 한자한문연구소 2020 동아한학연구 Vol.- No.14

NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma

Kim Jeongha,Kang Hyunkoo,Son Beomseok,Kim Min-Jung,Kang JiHoon,Park Kang Hyun,Jeon Jaewan,Jo Sunmi,Kim Hae Yu,Youn HyeSook,Youn BuHyun 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Overcoming therapeutic resistance in glioblastoma (GBM) is an essential strategy for improving cancer therapy. However, cancer cells possess various evasion mechanisms, such as metabolic reprogramming, which promote cell survival and limit therapy. The diverse metabolic fuel sources that are produced by autophagy provide tumors with metabolic plasticity and are known to induce drug or radioresistance in GBM. This study determined that autophagy, a common representative cell homeostasis mechanism, was upregulated upon treatment of GBM cells with ionizing radiation (IR). Nuclear receptor binding factor 2 (NRBF2)—a positive regulator of the autophagy initiation step—was found to be upregulated in a GBM orthotopic xenograft mouse model. Furthermore, ATP production and the oxygen consumption rate (OCR) increased upon activation of NRBF2-mediated autophagy. It was also discovered that changes in metabolic state were induced by alterations in metabolite levels caused by autophagy, thereby causing radioresistance. In addition, we found that lidoflazine—a vasodilator agent discovered through drug repositioning—significantly suppressed IR-induced migration, invasion, and proliferation by inhibiting NRBF2, resulting in a reduction in autophagic flux in both in vitro models and in vivo orthotopic xenograft mouse models. In summary, we propose that the upregulation of NRBF2 levels reprograms the metabolic state of GBM cells by activating autophagy, thus establishing NRBF2 as a potential therapeutic target for regulating radioresistance of GBM during radiotherapy.

Rhamnetin and Cirsiliol Induce Radiosensitization and Inhibition of Epithelial-Mesenchymal Transition (EMT) by miR-34a-mediated Suppression of Notch-1 Expression in Non-small Cell Lung Cancer Cell Lines

Kang, JiHoon,Kim, EunGi,Kim, Wanyeon,Seong, Ki Moon,Youn, HyeSook,Kim, Jung Woo,Kim, Joon,Youn, BuHyun American Society for Biochemistry and Molecular Bi 2013 The Journal of biological chemistry Vol.288 No.38

<P>Radioresistance is a major cause of decreasing the efficiency of radiotherapy for non-small cell lung cancer (NSCLC). To understand the radioresistance mechanisms in NSCLC, we focused on the radiation-induced Notch-1 signaling pathway involved in critical cell fate decisions by modulating cell proliferation. In this study, we investigated the use of Notch-1-regulating flavonoid compounds as novel therapeutic drugs to regulate radiosensitivity in NSCLC cells, NCI-H1299 and NCI-H460, with different levels of radioresistance. Rhamnetin and cirsiliol were selected as candidate Notch-1-regulating radiosensitizers based on the results of assay screening for activity and pharmacological properties. Treatment with rhamnetin or cirsiliol reduced the proliferation of NSCLC cells through the suppression of radiation-induced Notch-1 expression. Indeed, rhamnetin and cirsiliol increased the expression of tumor-suppressive microRNA, miR-34a, in a p53-dependent manner, leading to inhibition of Notch-1 expression. Consequently, reduced Notch-1 expression promoted apoptosis through significant down-regulation of the nuclear factor-κB pathway, resulting in a radiosensitizing effect on NSCLC cells. Irradiation-induced epithelial-mesenchymal transition was also notably attenuated in the presence of rhamnetin and cirsiliol. Moreover, an <I>in vivo</I> xenograft mouse model confirmed the radiosensitizing and epithelial-mesenchymal transition inhibition effects of rhamnetin and cirsiliol we observed <I>in vitro</I>. In these mice, tumor volume was significantly reduced by combinational treatment with irradiation and rhamnetin or cirsiliol compared with irradiation alone. Taken together, our findings provided evidence that rhamnetin and cirsiliol can act as promising radiosensitizers that enhance the radiotherapeutic efficacy by inhibiting radiation-induced Notch-1 signaling associated with radioresistance possibly via miR-34a-mediated pathways.</P>

Comparison of dietary food and nutrient intakes by supplement use in pregnant and lactating women in Seoul

Hyesook Kim,Won Jang,Ki-Nam Kim,Ji-Yun Hwang,Hae-Kyung Chung,Eun-Ju Yang,Hye-Young Kim,Jin-Hee Lee,Gui-Im Moon,Jin-Ha Lee,Tae-Seok Kang,Namsoo Chang 한국영양학회 2013 Nutrition Research and Practice Vol.7 No.3

This study was performed to compare the dietary food and nutrient intakes according to supplement use in pregnant and lactating women in Seoul. The subjects were composed of 201 pregnant and 104 lactating women, and their dietary food intake was assessed using the 24-h recall method. General information on demographic and socioeconomic factors, as well as health-related behaviors, including the use of dietary supplements, were collected. About 88% and 60% of the pregnant and lactating women took dietary supplements, respectively. The proportion of dietary supplements used was higher in pregnant women with a higher level of education. After adjusting for potential confounders, among the pregnant women, supplement users were found to consume 45% more vegetables, and those among the lactating women were found to consume 96% more beans and 58% more vegetables. The intakes of dietary fiber and β-carotene among supplement users were higher than those of non-users, by 23% and 39%, respectively. Among pregnant women, the proportion of women with an intake of vitamin C (from diet alone) below the estimated average requirements (EAR) was lower among supplement users [users (44%) vs. non-users (68%)], and the proportion of lactating women with intakes of iron (from diet alone) below the EAR was lower among supplement users [usesr (17%) vs. non-users (38%)]. These results suggest that among pregnant and lactating women, those who do not use dietary supplements tend to have a lower intake of healthy foods, such as beans and vegetables, as well as a lower intake of dietary fiber and β-carotene, which are abundant in these foods, and non-users are more likely than users to have inadequate intake of micro-nutrient such as vitamin C and iron.

Recombinant human KAI1/CD82 attenuates M1 macrophage polarization on LPS-stimulated RAW264.7 cells via blocking TLR4/JNK/NF-κB signal pathway

Hyesook Lee,Jung-Hwa Han,Kangbin An,Yun Jeong Kang,Hyun Hwangbo,Ji Hye Heo,Byung Hyun Choi,Jae-Joon Kim,Seo Rin Kim,Soo Yong Lee,Jin Hur 생화학분자생물학회 2023 BMB Reports Vol.56 No.6

KAI1/CD82, a membrane tetraspanin protein, can prevent variouscancers and retinal disorders through its anti-angiogenicand anti-metastatic capacity. However, little is known about itsanti-inflammatory effect and molecular mechanism. Therefore,the present study aimed to inLPSvestigate effect of a recombinantprotein of the large extracellular domain of human KAI1(Gly 111-Leu 228, rhKAI1) on lipopolysaccharides (LPS)-stimulatedRAW264.7 macrophage-like cells and mouse bone marrow-derived macrophages (BMDM) and to identify its underlyingmechanism. Our data showed that rhKAI1 suppressedexpression levels of classically macrophages (M1) phenotyperelatedsurface markers F4/80+CD86+ in LPS-stimulated BMDMand RAW264.7 cells. In addition, LPS markedly increased mRNAexpression and release levels of pro-inflammatory cytokinesand mediators such as interleukin (IL)-1β, IL-6, tumor necrosisfactor-α, cyclooxygenase-2, nitric oxide and prostaglandin E2,whereas these increases were substantially down-regulated byrhKAI1. Furthermore, LPS strongly increased expression of NF-κBp65 in the nuclei and phosphorylation of ERK, JNK, and p38MAPK. However, nuclear translocation of NF-κB p65 and phosphorylationof JNK were greatly reversed in the presence ofrhKAI1. Especially, rhKAI1 markedly suppressed expression oftoll-like receptor (TLR4) and prevented binding of LPS withTLR4 through molecular docking predict analysis. Importantly,Glu 214 of rhKAI1 residue strongly interacted with Lys 360 ofTLR4 residue, with a binding distance of 2.9 Å. Taken together,these findings suggest that rhKAI1 has an anti-inflammatoryeffect on LPS-polarized macrophages by interacting withTLR4 and down-regulating the JNK/NF-κB signaling pathway.

Screening of Myxobacteria Carrying Tubulysin Biosynthetic Genes

( Hyesook Hyun ),( Juo Choi ),( Daun Kang ),( Yungpil Kim ),( Pilgoo Lee ),( Gregory J. Y. Chung ),( Kyungyun Cho ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 한국미생물·생명공학회지 Vol.49 No.1

Tubulysins are a group of secondary metabolites produced by myxobacteria that inhibit the function of the eukayotic cytoskeleton. We developed a pair of PCR primers that specifically amplified tubulysin biosynthetic genes. Using these primers, eight out of the eighty-one strains of myxobacteria belonging to the Cystobacteraceae family that harbored putative tubulysin biosynthetic genes were screened through PCR analysis. The selected strains included two Archangium gephyra, two Stigmatella sp., two Vitiosangium cumulatum, and two unidentified myxobacteria. LC-MS analysis of the culture extracts from the selected strains revealed that A. gephyra KYC4066 produced putative tubulysin A and B.

Role of Metabolic Reprogramming in Epithelial–Mesenchymal Transition (EMT)

Kang, Hyunkoo,Kim, Hyunwoo,Lee, Sungmin,Youn, HyeSook,Youn, BuHyun MDPI 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.8

<P>Activation of epithelial–mesenchymal transition (EMT) is thought to be an essential step for cancer metastasis. Tumor cells undergo EMT in response to a diverse range of extra- and intracellular stimulants. Recently, it was reported that metabolic shifts control EMT progression and induce tumor aggressiveness. In this review, we summarize the involvement of altered glucose, lipid, and amino acid metabolic enzyme expression and the underlying molecular mechanisms in EMT induction in tumor cells. Moreover, we propose that metabolic regulation through gene-specific or pharmacological inhibition may suppress EMT and this treatment strategy may be applied to prevent tumor progression and improve anti-tumor therapeutic efficacy. This review presents evidence for the importance of metabolic changes in tumor progression and emphasizes the need for further studies to better understand tumor metabolism.</P>

일부 사업장에서 나타난 배치전건강진단 제도 현황과 향후과제

박혜숙 ( Hyesook Park ),강성규 ( Seong-kyu Kang ),이완형 ( Wanhyung Lee ),최원준 ( Won-jun Choi ),함승헌 ( Seunghon Ham ) 한국산업보건학회 2021 한국산업보건학회지 Vol.31 No.4

Introduction: Preplacement health examination (PHE) is performed when a worker starts a certain work task which is designated as having occupational risks by the Ministry of Employment and Labor (MOEL). All data related to health examination except PHE are reported to the MOEL by the law. This study has been performed to understand the status of PHE at certain workplaces. Methods: PHE data gathered in a university hospital were analyzed and they were followed with results of the special health examination (SHE) in 2019 and 2020. Those who were evaluated as unfit to work as it was, were interviewed directly or indirectly through an occupational health manager to follow up the management status of their recognized health problems. Results: The unfit to work (unFTW) rate of PHE was 2.8%, and was not different according to the size of workplace or having occupational health service. The major cause of unfit to work was the uncontrolled life-style diseases such as hypertension and diabetes. The rate of SHE followed by PHE was 31.1%. It was not different by the unFTW rates, however, they were different according to having a full time in-house occupational health manager. Thirty-one among 71 examinees who were evaluated as unFTW underwent SHE after controlling their health condition and were finally evaluated as fit to work. Nineteen among 31 started to take medicine and eight have been placed in the work without designated risks. Conclusion: PHE can be used for new workers, who may have unknown or uncontrolled life-style diseases, to be asked to manage life-style diseases as well as work-related risks such as shift work. In order to have a better tracking system for work-related risks, the information of PHE should be analyzed together with other data from health examination.

과학교육에서 인성교육의 필요성 및 인성의 구인에 대한 전문가의 인식

강유진 ( Eugene Kang ),전란영 ( Ranyeong Jeon ),김지나 ( Jina Kim ),김희화 ( Heehwa Kim ),박종석 ( Jongseok Park ),손정우 ( Jeongwoo Son ),조혜숙 ( Hyesook Cho ),남정희 ( Jeonghee Nam ) 한국과학교육학회 2018 한국과학교육학회지 Vol.38 No.4

인성교육은 주로 도덕교과에서 교육하는 주제로 인식되어져 왔지만, 최근에는 다른 교과에서도 통합적으로 교육되어야 한다는 주장이 제기되었다. 과학교과에서도 인성교육에 대한 몇 가지 시도가 이루어지고 있으며, 과학교과 고유의 인성교육의 필요성이나 인성구인에 대한 연구가 필요한 상황이다. 본 연구에서는 중등학교 교사, 과학교과 전공 대학교수, 및 연구소 연구원을 포함한 과학교육 전문가들을 대상으로 설문조사와 포커스 인터뷰를 통해서 인성의 필요성 및 인성 구인에 대한 인식을 조사하였다. 전문가들은 과학자들의 활동에 내재된 인성, 학교현장의 요구, 탐구적 지식 습득을 위해 과학교육에서 인성교육이 필요하다고 인식하였다. 인성의 도덕교육적 측면과 시민교육적 측면을 바탕으로, 본 연구에서 본질적 인성의 구인 4가지(정직, 공감, 관용, 자아존중), 실천적 인성의 구인 7가지(개인적 차원; 성실, 책임, 자기조절: 사회적 차원; 배려, 개방성, 소통, 협력)를 제시하였는데, 전문가들로부터 타당성과 중요도를 인정받았다. Recently it has been claimed that character education should be applied not only in ethics but also in other disciplines including science education. With several attempts at character education in previous research on science education, the examination of its requisite and components is needed. This research investigates the necessity and elements of character education to be improved upon in science classes through questionnaires and focus interviews of experts in the field of science education such as secondary school teachers, professors, and researchers. They recognize that character education should be adopted in science education with three backgrounds: scientist activities, the field of secondary school, and inquiry education. Based on moral education and citizenship, elements of character education in science class are comprised of four essential character elements including honesty, sympathy, tolerance, and self-esteem, and seven practical character elements that are divided into personal qualities: sincerity, responsibility, and self-control and social qualities: consideration, openness, communication, and cooperation. Their validity and importance are accepted by experts.