Surgical experience and clinical outcome of traumatic pancreatic injury

Hyeok Jo Kang,Sae Byeol Choi,Sang Yong Choi 한국간담췌외과학회 2012 한국간담췌외과학회지 Vol.16 No.4

Backgrounds/Aims: Traumatic pancreatic injury is rare and various surgical procedures can be applied according to the severity of injury. We reviewed our experience of pancreatic injury and investigated the clinical outcome. Methods: Fifty-six patients were treated conservatively or with surgery for pancreatic injury at the Department of Surgery, Korea University Medical Center of Korea University College of Medicine from January 2001 to February 2012. Results: Forty-one men and 15 women were included (mean age, 32 years; range, 5-66 years). Twelve patients were hypotensive at admission. According to the American Association for the Surgery of Trauma grade, 15 patients were grade Ⅰ, 16 were grade Ⅱ, 10 were grade Ⅲ, 13 were grade Ⅳ, and one patient was grade Ⅴ. A total of 41 patients underwent exploratory surgery. Complications developed in 35 patients, and 19 patients demonstrated intra-abdominal abscesses associated with pancreatic leakage. Four mortalities occurred. More adult patients (n=42) required intensive care than that of pediatric patients (n=14) (ρ=0.03). However, more pediatric patients had hyperamylasemia at admission (ρ=0.023). A significantly higher proportion of patients in the hypotensive group had blunt abdominal injuries, associated extra-abdominal injuries, combined intra-abdominal injuries, longer ICU stays, and a higher mortality rate. Conclusions: Associated intra-abdominal and extra-abdominal injuries are frequent in patients with traumatic pancreatic injury. Despite the complication rate, most patients recovered. Mortalities were associated with combined injuries being placed into bleeding, hypovolemic shock, and multiorgan failure. (Korean J Hepatobiliary Pancreat Surg 2012;16:160-166)

Controlling Solid-Electrolyte-Interphase Layer by Coating P-Type Semiconductor NiO_<i>x</i> on Li₄Ti₅O₁₂ for High-Energy-Density Lithium-Ion Batteries

Jo, Mi Ru,Lee, Gi-Hyeok,Kang, Yong-Mook American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.50

<P>Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> is a promising anode material for rechargeable lithium batteries due to its well-known zero strain and superb kinetic properties. However, Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> shows low energy density above 1 V vs Li<SUP>+</SUP>/Li. In order to improve the energy density of Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB>, its low-voltage intercalation behavior beyond Li<SUB>7</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> has been demonstrated. In this approach, the extended voltage window is accompanied by the decomposition of liquid electrolyte below 1 V, which would lead to an excessive formation of solid electrolyte interphase (SEI) films. We demonstrate an effective method to improve electrochemical performance of Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> in a wide working voltage range by coating Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> powder with p-type semiconductor NiO<SUB><I>x</I></SUB>. Ex situ XRD, XPS, and FTIR results show that the NiO<SUB><I>x</I></SUB> coating suppresses electrochemical reduction reactions of the organic SEI components to Li<SUB>2</SUB>CO<SUB>3</SUB>, thereby promoting reversibility of the charge/discharge process. The NiO<SUB><I>x</I></SUB> coating layer offers a stable SEI film for enhanced rate capability and cyclability.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2015/aamick.2015.7.issue-50/acsami.5b10207/production/images/medium/am-2015-102079_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am5b10207'>ACS Electronic Supporting Info</A></P>

Methionine Analogue Probes Functionally Important Residues in Active Site of Methionyl-tRNA Synthetase

Jo, Yeong-Joon,Lee, Sang-Won,Jo, Myung-Kyun,Lee, Jee-Woo,Kang, Mee-Kyoung,Yoon, Jeong-Hyeok,Kim, Sung-Hoon Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

Aminoacyl-tRNA synthetases are essential enzymes catalyzing the attachment of specific amino acids to cognate tRNAs. In the present work, the substrate analogue L-methionine hydroxamate was used to identify functional residues located in the active site of the E. coli methionyl-tRNA synthetase (MetRS). This compound inhibited bacteria, yeast, and human MetRS activities to a similar degree, suggesting a conserved active site structure and mechanism between MetRSs of different phylogenetic domains. Mutants of the E. coli MetRS resistant to methionine hydroxamate were also isolated. These mutants contained a substitution either at T10, Y15, or Y94. These residues are highly conserved among the different MetRSs and the mutants showed decreased aminoacylation activity, suggesting their functional and structural significances. The putative roles of these residues are discussed on a structural basis.

Fe₃O₄ nanoparticles encapsulated in one-dimensional Li₄Ti₅O₁₂ nanomatrix: An extremely reversible anode for long life and high capacity Li-ion batteries

Jo, Mi Ru,Jung, Jaepyeong,Lee, Gi-Hyeok,Kim, Yunok,Song, Kyeongse,Yang, Junghoon,Chae, Ji Su,Roh, Kwang Chul,Kim, Yong-Il,Yoon, Won-Sub,Kang, Yong-Mook Elsevier 2016 Nano energy Vol.19 No.-

<P><B>Abstract</B></P> <P>Transition metal oxides are very promising electrode materials for lithium-ion batteries that operate through conversion reactions. Energy densities for conversion reactions are higher than for intercalation reactions, but most of transition metal oxides show poor cycling performance and reversibility due to the pulverization of active materials and subsequent volume changes. We here report a facile and scalable synthesis for realizing Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB>-coated Fe/Fe<SUB>3</SUB>O<SUB>4</SUB> hybrid nanocomposites in the form of one-dimensional nanofibers (C@Fe-Fe<SUB>3</SUB>O<SUB>4</SUB>/Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> hybrid NFs). This is a new class of highly-reversible and safe anode material that can significantly reduce the lithium-ion diffusion length and improve strain tolerance during Li ion insertion/extraction. Its oxidation state was also impressively controlled through a carbothermal reaction during annealing. The precise oxidation state control of C@Fe-Fe<SUB>3</SUB>O<SUB>4</SUB>/Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> hybrid NFs simultaneously enabled high capacity due to the conversion reaction of Fe<SUB>3</SUB>O<SUB>4</SUB> as well as high reversibility and stability resulting from zero-strain characteristics and superb kinetics of Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB>. This new electrode material appears promising for not only future energy systems but also various electronic devices.</P> <P><B>Highlights</B></P> <P> <UL> <LI> C@Fe-Fe<SUB>3</SUB>O<SUB>4</SUB>/Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> hybrid NFs showed extremely stable cyclic retention, which enabled to realize a full cell based on transition metal oxides anode for the first time. </LI> <LI> Its mechanical flexibility, electric conductivity and thermal stability were surprisingly enhanced. </LI> <LI> This enhancement was attributed to zero-strain feature of Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB>, and the evolution of Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12-δ</SUB> and metallic Fe. </LI> <LI> This novel hybrid composites showed a synergistic effect thanks to high capacity of Fe<SUB>3</SUB>O<SUB>4</SUB>, high thermal and structural stability of Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB>, and the improved kinetics from dexterous oxidation state and morphology control. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>An effective strategy to significantly boost the structural stability and electrochemical performance of Fe<SUB>3</SUB>O<SUB>4</SUB> nanoparticles through the embedment in Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> nanomatrix was successfully demonstrated despite its extremely irreversible conversion reaction. The resultant C@Fe-Fe<SUB>3</SUB>O<SUB>4</SUB>/Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB> hybrid NFs showed excellent mechanical flexibility and electronic conductivity that could be each assigned to high stability or zero-strain feature of Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12</SUB>, and the evolution of oxygen-deficient Li<SUB>4</SUB>Ti<SUB>5</SUB>O<SUB>12-δ</SUB> as well as metallic Fe</P> <P>[DISPLAY OMISSION]</P>

Label-free identification of individual bacteria using Fourier transform light scattering.

Jo, YoungJu,Jung, JaeHwang,Kim, Min-Hyeok,Park, HyunJoo,Kang, Suk-Jo,Park, YongKeun Optical Society of America 2015 Optics express Vol.23 No.12

<P>Rapid identification of bacterial species is crucial in medicine and food hygiene. In order to achieve rapid and label-free identification of bacterial species at the single bacterium level, we propose and experimentally demonstrate an optical method based on Fourier transform light scattering (FTLS) measurements and statistical classification. For individual rod-shaped bacteria belonging to four bacterial species (Listeria monocytogenes, Escherichia coli, Lactobacillus casei, and Bacillus subtilis), two-dimensional angle-resolved light scattering maps are precisely measured using FTLS technique. The scattering maps are then systematically analyzed, employing statistical classification in order to extract the unique fingerprint patterns for each species, so that a new unidentified bacterium can be identified by a single light scattering measurement. The single-bacterial and label-free nature of our method suggests wide applicability for rapid point-of-care bacterial diagnosis.</P>

Acid treatment enhances performance of beads activated carbon for formaldehyde removal

Kang Yu-Jin,Jo Hyung-Kun,Jang Min-Hyeok,Han Gyoung-Jae,Yoon Seong-Jin,Oh Kyeongseok,Park Joo-Il 한국탄소학회 2023 Carbon Letters Vol.33 No.2

This study evaluated how acid treatment affects the ability of customized beads of activated carbon (BAC) to remove formaldehyde from air. Two different acids (hydrofluoric acid and sulfuric acid) were used to modify the surface of BAC prepared from a polymer material. The acid-modified BACs were further subjected to heat treatment. Physical and chemical characteristics of modified and unmodified BACs were investigated using nitrogen adsorption, Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray fluorescence, and X-ray photoelectron spectroscopy. Formaldehyde removal was evaluated under both dry and moist conditions. From the results, acid treatment clearly improved the adsorption performance, especially under the moist condition. Qualitative and quantitative surface analyses were conducted, mainly to examine the amount of O-bonds after acid treatment and the formation of S–O or Cl–O on BAC.

항암화학요법을 받는 암 환자에 대한 Nucare^R의 영양지지효과

조덕연,김현수,곽상혁,강정현,김철희,배광봉,김종숙,박상준,윤환중,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Purpose : The purpose of this study was to evaluate the effect of nutritional support with enteral liquid supplement in cancer patients receving chemotherapy for possible benefit in nutritional, immunologic and golbal function of patients. Patients and Methods : From October 1995 to February 1997, 30 advanced cancer patients receving chemotherapy were divided two roups. The Nycare group, in addition to normal diet, Nucare^R enteral supplement was given for 1week right after chemotherapy for the duration of 2 chemotherapy cycles. Control group received only normal diet without parenteral fluid supplement for 2 chemotherapy cycles. Results : Median ages were 53 end 56 years for Nucare group and Contrl group respectively. Performance scores was less than 2 by ECOG scale. All patients were stage Ⅲ or Ⅳ. The physical parameters, such as weight, arm muscle circumference(AMC) and triceps skin fold(TSF) were decreased in both groups after 2 cycles of anticancer therapy. but it was less severe in Nucare group(p<0.05). Serum transferrin was maintained in mild deficit state in Nucare group, whereas it aggravated form mild to moderate deficit in Control group(p<0.05). Serum albumin level increased in Nucare group without statistical significance. but it decreased from normal to mild deficit in Control group. Serum total protein did not change significantly in Nucare group. but in Control group, serum total protein was decreased from 7.24±0.9 to 6.52±0.5(P<0.05). Total lymphocyte count did not change significantly in both groups. Conclusion : This study shows that the nutritional support with Nucare^R was effective in the prevention of nutritional deficit status in patients receving a nticancer chemotherapy.

Exendin-4 inhibits interleukin-1beta-induced iNOS expression at the protein level, but not at the transcriptional and posttranscriptional levels, in RINm5F beta-cells.

Kang, Jung-Hoon,Chang, Seo-Yoon,Jang, Hyun-Jong,Kim, Dong-Bin,Ryu, Gyeong Ryul,Ko, Seung Hyun,Jeong, In-Kyung,Jo, Yang-Hyeok,Kim, Myung-Jun Journal of Endocrinology, Ltd. [etc.] 2009 The Journal of endocrinology Vol.202 No.1

<P>Cytokines such as interleukin-1beta (IL-1beta) stimulate inducible nitric oxide synthase (iNOS) expression and nitric oxide overproduction leading to beta-cell damage. Meanwhile, glucagon-like peptide-1 (GLP-1) and its potent analog exendin-4 (EX-4) were well known for beta-cell proliferation. However, the protective mechanisms of GLP-1 in beta-cells exposed to cytokines were not fully elucidated. Therefore, the effects of EX-4 on the IL-1beta-induced iNOS gene expression were investigated employing RINm5F beta-cells. EX-4 inhibited IL-1beta-induced iNOS protein expression and nitrite production. However, northern blot and promoter analyses showed that EX-4 failed to inhibit IL-1beta-induced iNOS mRNA expression and iNOS promoter activity. By electrophoretic mobility shift assay (EMSA), EX-4 did not alter the binding activity of NF-kappaB to the iNOS promoter. Consistent with the EMSA result, EX-4 did not inhibit nuclear translocation of p65. We also tested the effect of EX-4 on iNOS mRNA stability. Actinomycin D chase experiments showed that EX-4 did not affect the decay rate of iNOS mRNA and the promoter assay using the construct containing 3'-untranslated region of iNOS showed that EX-4 did not alter the stability of iNOS mRNA. Meanwhile, forskolin significantly inhibited IL-1beta-induced iNOS protein, which was reversed by H-89, a protein kinase A (PKA) inhibitor. Moreover, EX-4 pretreatment restored IL-1beta-induced decrease in cAMP toward control level. Additionally, the cycloheximide chase study demonstrated that EX-4 significantly accelerated iNOS protein degradation. We therefore concluded that EX-4 inhibited IL-1beta-induced iNOS protein and nitrite production via cAMP/PKA system irrespective of both transcriptional and posttranscriptional mechanisms of iNOS gene, and this inhibitory effect of EX-4 appears to be regulated at posttranslational level.</P>

Radial multi-quantum well ZnO nanorod arrays for nanoscale ultraviolet light-emitting diodes

Kang, Jang-Won,Kim, Byeong-Hyeok,Song, Hui,Jo, Yong-Ryun,Hong, Sang-Hyun,Jung, Gun Young,Kim, Bong-Joong,Park, Seong-Ju,Cho, Chang-Hee Royal Society of Chemistry 2018 Nanoscale Vol.10 No.31

<P>Since semiconducting ZnO has attractive properties such as wide bandgap and large exciton binding energy, it has motivated us to realize efficient ultraviolet (UV) light-emitting diodes (LEDs). Furthermore, facile growth of ZnO nanostructures has triggered numerous research studies to examine them as nanoscale building blocks for optoelectronic devices. Here, we demonstrate the growth of ZnO-based core-shell p-n homojunction nanorod arrays with radial MgZnO/ZnO multiple quantum wells (MQWs) and report the characteristics of a core-shell ZnO nanorod LED. The shell layers of MgZnO/ZnO MQWs and p-type antimony-doped MgZnO were epitaxially grown on the surface of ZnO core nanorod arrays. By introducing the radial MQWs, the photoluminescence intensity was greatly increased by 4 times, compared to that of the bare ZnO nanorod array, suggesting that the core-shell MQWs can be used to realize the nanoscale ZnO LEDs with high internal quantum efficiency. As the injection current increased, the EL intensity of UV emission at 375 nm from the MgZnO/ZnO MQWs strongly increased without shifting of the emission peak because of the non-polar nature of MQWs grown on the side walls of the ZnO nanorods. These results highlight the potential of an integrated nanoscale UV light emitter in various photonic devices.</P>

CCAAT box is required for the induction of human thrombospondin-1 gene by trichostatin A

Kang, Jung-Hoon,Kim, Myung-Jun,Chang, Seo-Yoon,Sim, Sang Soo,Kim, Myung-Suk,Jo, Yang-Hyeok Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of cellular biochemistry Vol.104 No.4

<P>Histone deacetylase (HDAC) inhibitors have been reported to inhibit angiogenesis as well as tumor growth. Thrombospondin-1 (TSP1) has been recognized as a potent inhibitor of angiogenesis. Such an action of TSP1 may account for the effect of HDAC inhibitors. In the present study, we investigated the molecular mechanism by which trichostatin A, a HDAC inhibitor, induces the expression of TSP1 gene. Trichostatin A increased both mRNA and protein levels of TSP1 in HeLa cells. Promoter and actinomycin D chase assays showed that trichostatin A-induced TSP1 expression was regulated at the transcriptional level without changing mRNA stability. CCAAT box on the TSP1 promoter was found to primarily mediate the trichostatin A response by deletion and mutation analyses of the TSP1 promoter. Electrophoretic mobility shift assay indicated that CCAAT-binding factor (CBF) was specifically bound to the CCAAT box of TSP1 promoter. Moreover, chromatin immunoprecipitation assay showed that trichostatin A increased the binding of acetylated form of histone H3 to the CCAAT box region of TSP1 promoter. Taken together, these results strongly suggest that trichostatin A activates the transcription of TSP1 gene through the binding of transcription factor CBF to CCAAT box and the enhanced histone acetylation. Thus, the present study provides the clue that the inhibition of angiogenesis by trichostatin A is accomplished through the upregulation of TSP1, the anti-angiogenic factor. J. Cell. Biochem. 104: 1192–1203, 2008. © 2008 Wiley-Liss, Inc.</P>