http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Synthesis of New Bis(amidine)–Cobalt Catalysts and Their Application to Styrene Polymerization
Cho, Wonseok,Cho, Hyemin,Lee, Chun Sun,Lee, Bun Yeoul,Moon, Bongjin,Kang, Jahyo American Chemical Society 2014 Organometallics Vol.33 No.7
<P>Two new bis(amidine) cobalt(II) complexes were rationally designed and efficiently synthesized. The first synthesized cobalt complex was based on a cyclic bis(amidine) ligand with chiral vicinal diphenyl groups. The structure was verified by single-crystal X-ray crystallography. As expected from the ligand structure, the Co–N bonds in this complex were significantly shorter than those found in the corresponding 1,2-diimine cobalt complex. This indicates that the bis(amidine) ligand has better electron-donating capability than the 1,2-diimine ligand. Upon test polymerization of styrene, the complex exhibited a moderate activity of 5.88 × 10<SUP>5</SUP> g PS/(mol Co h). On the basis of this encouraging result, a new 1,2-diaminobenzene-derived bis(amidine) ligand was efficiently synthesized and used to make the corresponding cobalt(II) complex. When subjected to styrene polymerization, the resulting complex showed unusually high polymerization activity [164 × 10<SUP>5</SUP> g PS/(mol Co h)] and high conversion (>99%). The resulting polymer was identified as atactic polystyrene by <SUP>13</SUP>C NMR spectroscopy analysis. The significantly enhanced styrene polymerization activity of the 1,2-diaminobenzene-originated bis(amidine) cobalt(II) complex is attributed to the improved electron-donating capability of the ligand.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orgnd7/2014/orgnd7.2014.33.issue-7/om401183d/production/images/medium/om-2013-01183d_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/om401183d'>ACS Electronic Supporting Info</A></P>
Molecular analysis of T-DNA insertion mutant population for transgenic rice plants
Hyemin Lim,A-Ram Kim,Jung-Il Cho,Hyeon-So Ji,Ung-Han Yoon,Tae-Ho Kim,Gang-Seob Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
We have generated 383 independent transgenic lines for genetically modified (GM) rice that contained GPD, UtrCSP, BrTSR15 and BrTSR53 genes overexpression constructs under the control of the constitutive CaMV 35S promoter. TaqMan copy number assay was determined inserted T-DNA copy number. Also FSTs analysis was isolated from 203 single copy T-DNA lines of transgenic plants and sequence mapped to the rice chromosomes. In analyzing single copy lines, we identified 95 FSTs, among which 37 (38.9%) were integrated into genic regions and 58 (61.1%) into intergenic regions. About 27 homozygous lines were obtained through multi-generations of planting, resistance screening and TaqMan copy number assay. To investigate the transgene expression patterns, quantitative real-time PCR analysis was performed using total RNAs from leaf tissue of single copy, intergenic region of T-DNA insertion and homozygous T2 plants. The mRNA expression levels of the examined transgenic rice were significantly increased in all of the transgenic plants. In addition, myc-tagged 35S::BrTSR15 and 35S::BrTSR53 transgenic plants were displayed higher levels of transgene protein than WT plants. These results may be useful for producing of large-scale transgenic plants or T-DNA inserted mutants in rice.
Generation and characterization of T-DNA insertion population for genetically-modified rice
Hyemin Lim,A-Ram Kim,Hyun-Ju Hwang,Jung-Il Cho,Hyeonso Ji,Chang-Kug Kim,Soo-Chul Park,Gang-Seob Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
We have generated 383 independent transgenic lines for genetically modified (GM) rice that contained PsGPD (Glyceraldehyde-3-Phosphate Dehydrogenase), ArCspA (Cold Shock Protein), BrTSR15 (Triple Stress Resistance 15) and BrTSR53 (Triple Stress Resistance 53) genes over-expression constructs under the control of the constitutive (CaMV 35S) promoter. TaqMan copy number assay was determined inserted T-DNA copy number. Also flanking sequence tags (FSTs) analysis was isolated from 203 single copy T-DNA lines of transgenic plants and sequence mapped to the rice chromosomes. In analyzing single copy lines, we identified 157 flanking sequence tags (FSTs), among which 58 (36%) were integrated into genic regions and 97 (62%) into intergenic regions. About 27 putative homozygous lines were obtained through multi-generations of planting, resistance screening and TaqMan copy number assay. To investigate the transgene expression patterns, quantitative real-time PCR analysis was performed using total RNAs from leaf tissue of single copy, intergenic region of T-DNA insertion and homozygous T2 plants. The mRNA expression levels of the examined transgenic rice were significantly increased in all of the transgenic plants. In addition, myc-tagged 35S:BrTSR15 and 35S:BrTSR53 transgenic plants were displayed higher levels of transgene protein. These results may be useful for producing of large-scale transgenic plants or T-DNA inserted mutants in rice.
조혜민(Hyemin Cho),이유민(Yumin Lee),장선미(Sunmee Jang) 대한약학회 2022 약학회지 Vol.66 No.5
Pharmacy utilization among immigrants in Korea is increasing amid the rapidly rising number of immigrants living in the country. However, few studies have investigated immigrants’ pharmacy utilization. This qualitative study used group interviews to explore immigrants’ experiences and perceptions of drug use and pharmacist services in Korea. The study participants consisted of 30 immigrants who had lived in Incheon and Gyeonggi for at least one year and had used pharmacies. The analysis results are grouped under three themes: “Reasons for visiting and selecting a pharmacy,” “Experience and perception of pharmacy and pharmacists’ services,” and “Experience and perception of taking medicine.” Our study shows that immigrants in Korea face language barriers when visiting pharmacies and struggle with Korea’s prescription-dispensing systems, forcing them to rely on their home country communities. However, immigrants who build a relationship of trust with a certain pharmacy tend to visit it regularly. Therefore, Korean pharmacists must strive to build good relationships with immigrants in order to help them adapt to Korea’s healthcare system.