Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

보리순의 영양성분 조성

서혜지,최용민,김상천,이준수,이기택,정명근,천지연,황진봉,남진식,서우덕,황영,김행란,김세나 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

한국인 주요우울장애 환자에서 혈중 ACTH, Cortisol 농도와 해밀턴 우울 평가 척도의 신체증상 항목과의 상관관계

여혜빈,김린,함병주,심세훈,권영준,정희연,정한용,한상우,이민수,이화영 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:Hypothalamic-Pituitary-Adrenocortical(HPA) system dysregulation plays an important role in the pathophysiology of major depressive disorder. Medically unexplained somatic symptoms comprised the predominant complaints of korean patients with major depressive disorder. It might be related to dysregulation of HPA system and somatic symptoms. The aim of this study was to investigate the association between Hamilton Depression Rating Scale severity and the Plasma Adrenocorticotropic Hormone(ACTH) & Serum Cortisol level in Korean patients with major depressive disorder. Methods:Our study design was prospective. A total 111 hospitalized patients with major depressive disorder (DSM-IV) at Anam Hospital, Korea University Medical Center between Jan 2009 and May 2011 were selected. Hamilton Depression Rating Scale was measured by psychiatrist. Plasma ACTH, Serum Cortisol levels were analyzed at the time of admission. Results:There are significant correlations(p<0.05) between Hamilton Depression Rating Scale of somatic component(HAM-D 11-15 item) and Plasma ACTH and Serum Cortisol level. However, other HAM-D sub items were not significantly correlated with Plasma ACTH and Serum Cortisol level except somatic component. Conclusion:Our Study results suggest that hyperactivity of HPA axis might be correlation with somatic symptoms in korean patients with major depressive disorder.

호텔업의 신사업 진출전략에 관한 연구

원융희,이미혜 용인대학교 산업경영연구소 2004 산업경영논총 Vol.11 No.-

The purpose of this study is to improve brand image and contribute to the growth of Hotels by suggesting appropriate business management strategies for the approach in the context of an managemental point of view in order to reflect the current trend of Hotel managements. In those days, Hotels are a deep competitive relation of others. Under the current situation, it is very important to activate and strengthen the competitiveness of Hotels. Therefore Hotels advance the new business outsides, specially is foodservice. At once, Hotels are inroading foodservie industry because maintances Sustainable Competitive advanges(SAC). The new business strategies of Hotels foodservices is suggested by three parts, which are cost efficiency, individualization, service quality. To achieve the purpose of this study, literature research and cases collects, and objective and scientific analysis are done.

청소년이 지각한 가족기능과 희망과의 관계

우선혜,노지영,박영숙 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.1

Purpose: This Study a descriptive correlation survey with the purpose of identifying the relationship between family function and hope in adolescents. It was done to provide basic data for developing family centered nursing strategies to promote adolescents' hope. Methods: The subjects of this study were 1030 high school students in J city. Data were analyzed with SPSS 10.0 program. Frequencies and percentages were used to compare the subjects' general characteristics. Minimum values, maximum values, mean scores and mean grades were used to identify the level of family function and hope. Pearson's correlation coefficient was used to analyze the relationship between family function and hope in adolescents, and t test, ANOVA, and Scheffe test were used to examine the level of family function and hope by general characteristics. Results: 1. The level of adolescents' family function ranged from 26 to 97, the mean score was 66.85±13.16, and the mean grade was 2.76±0.52. 2. The level of adolescents' hope ranged from 55 to 153, the mean score was 111.48±17.64 and the mean grade was 2.78±0.44. 3. It showed that there is a significantly positive correlation (r=0.546, p=.000) between adolescents' family function and hope. That is, the better family function adolescents have, the higher level of hope they have. 4. Adolescents' family function showed significant differences according to school record (F=6.363, p=.002), school life satisfaction (F=4.922, p=.007), father's education (F=2.640, p=.048), mother's education (F=3.586, p=.013) and domestic economy status(F=3.022, p=.049). 5. Adolescents' hope showed significant differences according to religion (t=2.018, p=.044), school type (t=2.567, p=.010), school record (F=9.712, p=.000), school life satisfaction (F=11.367,p=.000), father's education (F=4.632, p=.003), mother's education (F=4.709, p=.003), domestic economy status (F=4.235, p=.015), relationship with friends (F=8.187, p=.000), and subjective health condition (F=5.490, p=.001). Conclusion: First, in order to improve adolescents' family function, which encourages hope in adolescents, it is necessary to develop nursing interventions and to make further studies. Second, it is necessary to develop instruments for improving Korean adolescents' family function and hope. Third, further studies are required to improve adolescents' family function and hope in special situations such as disease, drug abuse and juvenile delinquent.

공황장애 환자에서 약물 치료와 심박동수 변이(Heart Rate Variability)의 관련성

우종민,김응석,윤혜영,최영희 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.3

Objectives : This study was designed to evaluate relation between 6 weeks pharmacotherapy and the alteration of autonomic nervous system in patient with panic disorder. Methods : The subjects were patients (n=44) who met DSM-IV criteha for panic disorder with or without agoraphobia. They were measured Anxiety Disorder Inventory Schedule-Panic attack & agoraphobia (ADIS-P & A), Clinical Global Impression (CGI) Hamilton Rating Scale for depression (HAM-D), Panic Disorder Severity Scale (PDSS) and heart rate Vahability (HRV) before and after 6 weeks pharmacotherapy. We prescribed paroxetine primahly and benzodiazepine (clonazepam, alprazolam) in case needed. For analysis, we performed paired sample t-test, partial correlation, stepwise multiple regression. Results : After 6 weeks pharmacotherapy, a assessed clinical inventories significant improved. In HRV, patients showed stabilized sympathetic activity (LF, Normalized LF, 1og_LF), increased parasympathetic activity (Normalized HF). There were significant correlation between symptom improvement with parasympathetic components and symptom aggravation with sympathetic components. Multiple regression analysis showed that Normalized HF and TP were best explanatory variables of symptom improvement. Conclusion : This study suggests that 6 weeks pharmacotherapy is related to the alteration of autonomic nervous system and clinical improvement.

小兒의 Guillain-Barre 症候群에 對한 臨床的 觀察

閔惠蘭,禹永琮,馬在淅 全南大醫大 1986 전남의대학술지 Vol.23 No.3

혈우병 환자의 임상 양상에 관한 단일기관 연구

허지혜,김형욱,김영대,이영호,김남수,설인준,김상우 인제대학교 2006 仁濟醫學 Vol.27 No.-

Objective : We analyzed and investigated clinical manifestations, laboratory tests, and inhibitor development of hemophilia that had not been thoroughly studied. Methods : We retrospectively reviewed 96 patients with hemophilia who were registered in the Hanyang University hospital from Jan, 1984 to Dec, 2003, Results : The total patients, whose median age at onset was 46.0 months, in clude 84 cases of type A (87.5%) and 12 cases of type B (12.5%). The most common chief complaint was a traumatic hemorrhage. The family histories of hemophilia were revealed in 41 cases (42.7%). The median activated partial thromboplastin time (aPTT) was 66.7 seconds. The number of severe cases was 39 (40.6%). Inhibitors were present in 13 cases (13.5%) and seven cases (53.8%) were high responders. aPTT and factor levels showed reverse correlation (r=-0.467). aPTT is more prolonged in group without family history than the group with family history (P=0.037). Conclusion : In this study, the diagnosis of hemophilia are made at the lower age and the serological positivity of the HCV is decreased. Activated partial thromboplastin time and factor levels shows reverse correlation. Activated partial thromboplastin time is more prolonged in group without family history compared with group with family history. The frequencies of family history are lower than those of other developed countries, because of concealing the family history of hemophilia. The prevalence of the development of inhibitors increased compared with those of previous studies in Korea, but was lower than those of other countries. Further studies would be necessary to decrease the inhibitor development.

까나리 염장품 추출물 저장 중의 품질 변화

이혜정,공용우,김종규 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.3

The quality changes of the extracts of salt-fermented Ammodytes personatus during the storage were examined at 1 month interval during 3 months fermentation period. To prepare the extracts of Ammodytes personnatus, 17%, 20% and 25% salt were added respectively and then stored for 3 months at room temperature and then extracted with hot water. During the storage, moisture and lipid contents of the samples decreased, but the crude protein contents increased gradually. On the other hand, ash content and pH showed little changes during the fermentation storage. After 6 months fermentation, the extracts of salt-fermented Ammodytes personatus were rich in free amino acids, such as glutamic acid, alanine, lysine, leucine, isoleucine, valine and aspartic acid in the order. As the fermentation progressed, the fish odor, turbidity, salty taste, savory flavor and overall preferences were similar to those of Ammodytes personatus sauce in the market. The total cell numbers were 450∼570 cfu/mL at 0 day, but there were none revealed during the fermentation.

호산구성 간농양 54예의 임상상

강혜련,권혁수,송우정,이상민,김태범,박혜경,장윤석,김윤근,조상헌,민경업,김유영 대한알레르기학회 2004 천식 및 알레르기 Vol.24 No.2