NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.

홍삼류의 섭취가 비만과 혈중 지질의 상호관계에 미치는 영향

박화진,이정희,이소진,함혜선,조현정,임창률,유영빈,박기현 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.4

비만은 동맥경화의 위험인자로서 혈중에 triglyceride의 농도를 종가시키고, 상대적으로 HDL-Cholesterol의 농도를 감소시킴과 동시에 수축기 혈압을 상승시킨다. 본 연구에서 홍삼제품류를 4년 또는 5년 동안 복용해 온 건강한 사람 (ginseng군)과 홍삼제품을 복용하지 않은 건강한 사람 (control군)을 대상으로 하여 신체 계측치로부터 비만지수를 구하고 이것과 혈중의 triglyceride (TG)농도, TG/HDL-Cholesterol ratio 및 수축기 혈압과의 상호관계를 연구한 바, ginseng군에서는 대조군에 비해 혈중의 TG 농도가 일정하게 유지되었고, TG/HDL-Cholesterol ratio 및 수축기 혈압도 일정하게 유지되었다. 이러한 현상은 ginseng군에서 기호품으로 alcohol을 섭취하거나, 흡연을 한 경우에도 상관없이 일정하게 유지 되었다. 이 결과는 홍삼제품류를 장기 복용하면 비만의 저하 및 고혈압 또는 동맥경화의 위험인자가 억제될 수 있고, 결론적으로 비만, 고혈압 및 동맥경화를 예방할 수 있을 것으로 추정한다. Obesity is the risk fcactor of atherosclerosis and not only increases triglyceride concentration in blood but also decreases relatively the ratio of TG to HDL-Cholesterol in blood. In case of obesity, systolic blood pressure is also increased in responding the increase of TG in blood. Index of obesity in red ginseng-taking group (ginseng group) was lower as compared with non-red ginseng-taking group (control group). The TG concentration, the ratio of triglyceride to HDL-cholesterol in blood and systolic blood pressure were decreased in the subjects of ginseng group compared with that in control group. It is inferred that long-term intake of ginseng products may help to prevent the risk of atherosclerosis and obesity.

The IL-10-producing regulatory B cells (B10 cells) and regulatory T cell subsets in neuromyelitis optica spectrum disorder

Cho, Eun Bin,Cho, Hye-Jin,Seok, Jin Myoung,Min, Ju-Hong,Kang, Eun-Suk,Kim, Byoung Joon Springer-Verlag 2018 NEUROLOGICAL SCIENCES Vol.39 No.3

<P>B cells contribute to the pathogenesis of neuromyelitis optica (NMO) by producing Aquaporin 4-specific autoantibodies (AQP4-ab); on the other hand, there are certain B cells that suppress immune responses by producing regulatory cytokines, such as IL-10. In this study, we investigated the presence of IL-10-producing Breg cells among lymphocyte subsets. Twenty-two seropositive NMO spectrum disorder (NMOSD) patients (29 samples) and 13 healthy controls (HCs) (14 samples) were enrolled. All NMOSD patients have received one or more immunosuppressive drugs. The phenotype and frequency of B cell and T cell subsets in the peripheral blood were measured by flow cytometry. We defined Breg cells as IL-10-producing B (B10) cells, which are CD19(+)CD39(+)CD1d(+)IL-10(+). The potential relations were evaluated between specific lymphocyte subsets and AQP4-ab intensity measured by the cell-based indirect immunofluorescence assay. The frequency of B10 cells was higher in patients with NMOSD regardless of the disease status than that in HCs (attack samples; p = 0.009 and remission samples; p < 0.001, respectively). In addition, the frequency of IL-17(+) Treg cells among Treg cells was higher during remission than during an attack (uncorrected p = 0.032). Among the lymphocyte subsets, B10 cells alone showed a positive correlation with the intensity of AQP4-ab positivity (rho [rho] = 0.402 and p = 0.031). It was suggested that the suppressive subsets including B10 and IL-17(+) Treg cells might have important roles in controlling disease status in NMOSD. Further functional studies may help to elucidate the immunological role of B10 and IL-17(+) Treg cells in NMOSD.</P>

Effect of the Position of Histidine Tag on Overproduction of Recombinant γ-Glutamyltranspeptidase in E. coli

Hye-Bin CHO,Sun-Hee YOON,Young-Wan KIM 한국생물공학회 2014 한국생물공학회 학술대회 Vol.2014 No.4

형태적 구성방법에 따른 전이공간에 관한 연구 -초등학교를 중심으로-

조혜빈(Cho, Hye-Bin),원호성(Won, Ho-Sung) 대한건축학회 2022 대한건축학회 학술발표대회 논문집 Vol.42 No.1

Industrialization and urbanization proceeded with technological development. The face of society is also constantly changing. As the way of life of human beings also changes, the space is also changing into various forms. Accordingly, the transition space is also changing. However, it is not simply a public space that connects private spaces, but is changing into a third space with its own character. The characteristics of the transition space were analyzed by dividing the design competition into three types, centering on the elementary school. The corridor, which used to be a moving space in the old school, has been transformed into a space with a new character. In addition, an integrated, intrusive, and overlapping space that is not partitioned into a square classroom was created. This means that changes have come to the transitional space of the educational space. Through this, it can be referred to in planning an efficient and creative transition space when planning the construction of educational facilities.

에너지 효율적 B5G 스몰셀 네트워크를 위한 강화학습 기반 이진 전송전력 제어 기법

조혜빈(Hye Bin Cho),이준승(Jun Seung Lee),김형섭(Hyung Sub Kim),나지현(Jee Hyeon Na),이호원(Howon Lee) 한국통신학회 2023 한국통신학회 학술대회논문집 Vol.2023 No.6

배지최적화를 통한 재조합 바실러스 서브틸리스에서 바실러스 아밀로리퀴파시엔스 유래 γ-글루타밀펩타이드전달효소의 대량생산

조혜빈(Hye-Bin Cho),제텐드라 쿠마르 로이(Jetendra Kumar Roy),박우진(Wu-Jin Park),전병운(Byoung-Oon Jeon),김영완(Young-Wan Kim) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.6

본 연구를 통해 BAGGT를 재조합 B. subtilis를 이용하여 대량 생산하기 위하여 유전자 클로닝, 발현 시스템 구축 및 배지 최적화를 진행하였다. 이중프로모터 시스템을 이용하여 야생형 균주에 비해 42배 효소 생산성이 향상된 발현 시스템을 구축하였다. 또한 PBD 분석을 통해 당밀과 CSL이 재조합 B. subtilis 시스템에서 BAGGT의 생산성에 큰 영향을 주는 인자임을 확인하였으며, 염류의 첨가에 의한 효소 생산성 증대 효과는 미비하거나 부정적이었다. 탄소원으로 당밀을 선택하고 고가의 질소원인 트립톤을 저가의 CSL로 교체한 후 CCD 분석을 통해서 결정된 최적 배지 사용 시 최적화 이전의 LB 배지 대비 4.3배의 생산성 증대를 이루었으며, 이는 LB 배지에서 야생형 균주의 BAGGT 생산성 대비 180배의 효소 생산성 개선에 해당하였다. 본 연구를 통해 식품용 효소로서 BAGGT의 대량생산을 위한 공정을 구축하였으며, 이후 정미성 소재 생산에 활용할 수 있을 것으로 기대한다. γ-Glutamyltranspeptidase (GGT, EC 2.3.2.2) transfers γ-glutamyl moiety from glutamine to amino acids or peptides and hydrolyzes glutamine to glutamate and ammonia. In order to overproduce γ-glutamyltranspeptidase from Bacillus amyloliquefaciens (BAGGT), the encoding gene was cloned and expressed in Bacillus subtilis. The productivity of BAGGT in Bacillus subtilis was improved by 42-fold by using a dual-promoter system that was generated by combining promoters from B. subtilis α-amylase and BAGGT genes. Through optimization of medium composition by Plackett-Burman design and central composition design, BAGGT was produced at 18.3×107 U/L of culture in the optimized medium. Compared to previously used Luria-Bertani medium, the optimized culture medium (15 g/L molasses, 60 g/L corn steep liquor, 6 g/L yeast extract, 4 g/L NaCl, 6 g/L K2HPO4, and 2 g/L KH2PO4), resulted in a 4.3-fold increase in production of BAGGT.

Types of Home Meal Replacement and Determinants of Consumption in South Korea

Kyeong Ah Ahn,Young Chan Choe,Hye Bin Cho 한국농식품정보과학회 2014 Agribusiness and Information Management Vol.6 No.2

HMR is a home-style food product designed for convenience and cooked outside the home leaving out cumbersome cooking process and consumed at home. The present paper aims to find out factors that influence the consumption of HMR by analyzing data on food consumption during the 3 years between December 2010 and November 2013. Following the classification of Costa et al. (2001), this study categorized HMR products as 3 types as follows: C1 (ready to eat), C2 (ready to heat) and C3 (ready to cook), and examined factors affecting purchase rate and per capita purchase price for each type of HMR product. The results of our analysis show that only the purchase rate of C3 products was influenced by whether the purchaser was housewife with job or not. For those who do not live together with parents, per capita purchase price for HMR was high; and the more they ate out, the higher the purchase rate of HMR was.

Early Onset Type 2 Diabetes Mellitus in Non-Obese Adolescents Born Small for Gestational Age

Hye Yeon Choi,Juyoung Lee,Danbi Kim,서진순,Joong Hyun Bin,이수영,Kyoung Soon Cho 대한신생아학회 2020 Neonatal medicine Vol.27 No.4

Being born small for gestational age (SGA) has been strongly associated with mortality during the perinatal period and long-term risk of metabolic syndrome, including type 2 diabetes mellitus, hypertension, hyperlipidemia, and coronary heart disease. Insulin resistance is an important factor in the development of metabolic syndrome in SGA, with several proposed hypotheses. Here, we report two cases of non-obese adolescent patients with early onset type 2 diabetes who were born SGA. Of these, one experienced catch-up growth, while the other did not. Both had a high body fat percentage at the time of diagnosis of type 2 diabetes and were diagnosed with fatty liver and hyperlipidemia before adolescence, at the age of 7 years. Early interventions for SGA are needed for healthy catch-up growth to prevent metabolic diseases in the future.

Expression of piezo2 in the human dental pulp and the sensory root of the rat trigeminal ganglion

Hye Min Han,Su Bin An,Jae Young Choi,Yi sul Cho,Yong Chul Bae 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.1