한국인 음주자들의 혈청 Gamma Glutamyl Transferase 활성도에 관한 연구

金輝俊,安智榮,金昌世 순천향대학교 1992 논문집 Vol.15 No.2

The enzyme r-glutamyl transpeptidase(GGT) catalyzes the hydrolysis of r-glutamyl peptides such as glutathione and the transfer of their r-glutamyl moieties to amino acids and peptides. Since GGT was discovered in 1950 by Hanes et al., it has been established as useful supportive liver function test in various hepatobiliary diseases. Since Zein et al. found in 1970 that serum GGt activities were markedly increased in chronic alcoholism, even though both transaminase and alkaline phosphatase(ALP) being normal, a number of studies have shown a elvations of GGT in alcoholism and good correlation between GGT and amount of alcohol intake. Recently, it has been suggested that serum GGT activity may have a value as a sereening test for alcoholism. This study was conducted on 459 male adults who were examined periodically for physical checkup at Chunan hospital of Soonchunhyang University College of Medicine in October, 1991, with structural questionaire about their drinking habits. Among 459 male adults serum GGT was determined in 236 healthy men consisted of 40 teetotalers and 196 drinkers assessed by history taking, physical examination, and liver function test. This study was attempted with following objectives : 1) to investigate a correlation between serum GGT activity and amount of alcohol intake(grams of pure ethanol per week) and between serum GGT activity and duration of alcohol intake by years, and 2) to investigate whether increased serum GGT activity reflects amount of alcohol intake and duration. The result obtained are as follows; 1) The means of serum GOT in 40 teetotalers and 196 healthy drinkers were 24.8±6.0 U/L and 25.1±5.3 U/L, respectively. The means of two groups showed no statistically significant difference at p >0.05. 2) The means of serum GPT in 40 teetotalers and 196 healthy drinkers were 23.7±8.2 U/L and 22.5±7.3 U/L, respectively. The means of two groups showed no statistically significant difference at p>0.05. 3) The means of serum GGT in 40 teetotalers and 196 healthy drinkers were 24.3±7.3 U/L and 38.9±20.3 U/L, respectively. The mean of serum GGT in healthy drinkers is higher than the mean of serum GGT in teetotalers. The means of two groups showed statistically significant difference at p<0.05. 4) In 196 healthy drinkers the increase of serum GGT activity was paralleled with increasing alcohol intake. Means of serum GGT activities of 5 groups classified by amount of alcohol intake showed statistically significant difference at p<0.05. But serum GGT activity was increased only in long-standing frinkers over 20 years according to duration of alcohol intake. 5) The probability of being a heavy drinker was increased with increasing serum GGT activity and presumably increased to 60% at 80U/L or more of serum GGT activity. 6) Serum GGT activity showed statistically significant correlation with amount of alcohol intake(r=0.3237) and weak correlation with duration of alcohol intake(r=1.1971).

Malassezia sympodialis가 동정된 신생아 Malassezia 농포증 1예

김휘준,이무형,안규중 대한의진균학회 2001 대한의진균학회지 Vol.6 No.4

Neonatal Malassezia pustulosis can be defined as pustules on face and neck, age at onset younger than 1 month, isolation of Malassezia by direct microscopy in pustular material, elimination of other causes of neonatal pustuloses, and response to topical ketoconazole therapy. We report a case of neonatal Malassezia pustulosis in a 20-day-old male. Direct microscopic examination on smears for pustules showed forms of Malassezia yeasts and culture yielded Malassezia sympodialis. The lesions were remarkably improved by topical ketoconazole cream for 14 days. [Kor J Med Mycol 6(4): 229-231] Key Words: Neonatal Malassezia pustulosis

인천 인현동 호프집 화재 피해자 분석

최정태,안무업,안희철,최영미,정재봉,서정열,유기철,이삼우,박석현,조준휘,김성환,김아진,송근정 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Purpose : This study was conducted to develop field triage, transportation, distribution, and prehospital care at a fire disaster by analyzing the victims of the fore that broke out at a bar in Incheon. Method : We analyzed the cases of the victims of a fire in Incheon in Oct. 1999. We determined the primary care hospital, the arrival time, the burn size, the outcome, and the injury type from the medical records, the concerned organ records, and interviews with concerned persons. Result : The total number of victims was 137: 56 prehospital deaths, 1 hospital death, and 80 survivals. The Pearson correlation coefficient between the burn size and the severity was -0.175. There were 121(89.6%) cases of inhalation injury, 59 (43.7%) cases of flame burns, 66 (48.9%) cases of hypoxic brain damage, and 16 (11.9%) cases involving other types of injury. Conclusion : The causes of death of the fire victims were inhalation injury and hypoxic brain damage due to CO poisoning and other toxic inhalants. We propose the use of a simple triage and rapid treatment(START) system and a reassessment the delayed category in fire disasters.

Early IV-injected human dermis-derived mesenchymal stem cells after transient global cerebral ischemia do not pass through damaged blood-brain barrier

Ahn, Ji Hyeon,Chen, Bai Hui,Park, Joon Ha,Shin, Bich Na,Lee, Tae-Kyeong,Cho, Jeong Hwi,Lee, Jae Chul,Park, Jeong-Ran,Yang, Se-Ran,Ryoo, Sungwoo,Shin, Myoung Cheol,Cho, Jun Hwi,Kang, Il Jun,Lee, Choong Wiley (John WileySons) 2018 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MED Vol.12 No.7

<P>There is lack of researches on effects of intravenously injected mesenchymal stem cells (MSCs) against transient cerebral ischemia (TCI). We investigated the disruption of the neurovascular unit (NVU), which comprises the blood-brain barrier and examined entry of human dermis-derived MSCs (hDMSCs) into the damaged hippocampal CA1 area in a gerbil model of TCI and their subsequent effects on neuroprotection and cognitive function. Impairments of neurons and blood-brain barrier were examined by immunohistochemistry, electron microscopy, and Evans blue and immunoglobulin G leakage. Neuronal death was observed in pyramidal neurons 5-day postischemia. NVU were structurally damaged; in particular, astrocyte end-feet were severely damaged from 2-day post-TCI and immunoglobulin G leaked out of the CA1 area 2days after 5min of TCI; however, Evans blue extravasation was not observed. On the basis of the results of NVU damages, ischemic gerbils received PKH2-transfected hDMSCs 3 times at early times (3hr, 2, and 5days) after TCI, and fluorescence imaging was used to detect hDMSCs in the tissue. PKH2-transfected hDMSCs were not found in the CA1 from immediate time to 8days after injection, although they were detected in the liver. Furthermore, hDMSCs transplantation did not protect CA1 pyramidal neurons and did not improve cognitive impairment. Intravenously transplanted hDMSCs did not migrate to the damaged CA1 area induced by TCI. These findings suggest no neuroprotection and cognitive improvement by intravenous hDMSCs transplantation after 5min of TCI.</P>

Activation of immediate-early response gene c-Fos protein in the rat paralimbic cortices after myocardial infarction

Ahn, Ji Yun,Tae, Hyun-Jin,Cho, Jeong-Hwi,Kim, In Hye,Ahn, Ji Hyeon,Park, Joon Ha,Kim, Dong Won,Cho, Jun Hwi,Won, Moo-Ho,Hong, Seongkweon,Lee, Jae-Chul,Seo, Jeong Yeol Medknow PublicationsMedia Pvt Ltd 2015 Neural regeneration research Vol.10 No.8

<P>c-Fos is a good biological marker for detecting the pathogenesis of central nervous system disorders. Few studies are reported on the change in myocardial infarction-induced c-Fos expression in the paralimbic regions. Thus, in this study, we investigated the changes in c-Fos expression in the rat cingulate and piriform cortices after myocardial infarction. Neuronal degeneration in cingulate and piriform cortices after myocardial infarction was detected using cresyl violet staining, NeuN immunohistochemistry and Fluoro-Jade B histofluorescence staining. c-Fos-immunoreactive cells were observed in cingulate and piriform cortices at 3 days after myocardial infarction and peaked at 7 and 14 days after myocardial infarction. But they were hardly observed at 56 days after myocardial infarction. The chronological change of c-Fos expression determined by western blot analysis was basically the same as that of c-Fos immunoreactivity. These results indicate that myocardial infarction can cause the chronological change of immediate-early response gene c-Fos protein expression, which might be associated with the neural activity induced by myocardial infarction.</P>

3 차원 레이저 가공기를 위한 전용 CAM 시스템 개발

김휘준(Simon Hwi-jun Kim),김형중(Hyung-Jung Kim),최원용(Paul Choi),김동수(Dong-Soo Kim),안성훈(Sung-Hoon Ahn),전차수(Cha-Soo Jun) (사)한국CDE학회 2007 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2007 No.1

Effects of long-term scopolamine treatment on cognitive deficits and calcium binding proteins immunoreactivities in the mouse hippocampus

Ahn, Ji Hyeon,Chen, Bai Hui,Yan, Bing Chun,Park, Joon Ha,Kang, Il Jun,Lee, Tae-Kyeong,Cho, Jeong Hwi,Shin, Bich-Na,Lee, Jae-Chul,Jeon, Yong Hwan,Hong, Seongkweon,Lee, Young Joo,Choi, Soo Young,Won, Mo SPANDIDOS PUBLICATIONS 2018 MOLECULAR MEDICINE REPORTS Vol.17 No.1

<P>GABAergic projections terminate on numerous hippocampal interneurons containing calcium binding proteins (CBPs), including calbindin D-28k (CB), calretinin (CR) and parvalbumin (PV). Memory deficits and expression levels of CB, CR, and PV were examined in the hippocampal subregions following systemic scopolamine (Scop; 1 mg/kg) treatment for 4 weeks in mice. Scop treatment induced significant memory deficits from 1 week after Scop treatment. CB, CR and PV immunoreactivities distributions were in hippocampal subregions [CA1 and CA3 regions, and the dentate gyrus (DG)]. CB immunoreactivity (CB<SUP>+</SUP>) was gradually decreased in all subregions until 2 weeks after Scop treatment, and CB<SUP>+</SUP> was decreased to the lowest level in all subregions at 3 and 4 weeks. CR<SUP>+</SUP> in the CA1 region was gradually decreased until 2 weeks and hardly observed at 3 and 4 weeks; in the CA3 region, CR<SUP>+</SUP> was not altered in all subregions at any time. In the DG, CR+ was gradually decreased until 2 weeks and lowest at 3 and 4 weeks. PV<SUP>+</SUP> in the CA1 region was not altered at 1 week, and gradually decreased from 2 weeks. In the CA3 region, PV<SUP>+</SUP> did not change in any subregions at any time. In the DG, PV<SUP>+</SUP> was not altered at 1 week, decreased at 2 weeks, and lowest at 3 and 4 weeks. In brief, Scop significantly decreased CBPs expressions in the hippocampus ≥3 weeks after the treatment although memory deficits had developed at 1 week. Therefore, it is suggested that Scop (1 mg/kg) must be systemically treated for ≥3 weeks to investigate changes in expression levels of CBPs in the hippocampus.</P>

Age-dependent decrease of Nurr1 protein expression in the gerbil hippocampus

Ahn, Ji Hyeon,Lee, Joon Seok,Cho, Jun Hwi,Park, Joon Ha,Lee, Tae-Kyeong,Song, Minah,Kim, Hyunjung,Kang, Seok Hoon,Won, Moo-Ho,Lee, Choong Hyun D.A. Spandidos 2018 Biomedical reports Vol.8 No.6

<P>Nuclear receptor related-1 protein (Nurr1) serves important roles in hippocampal-dependent cognitive process. In the present study, the protein expression of Nurr1 was compared in the hippocampi of young [postnatal month 3 (PM 3)], adult (PM 12) and aged (PM 24) gerbils using western blot analysis and immunohistochemistry. Results indicated that the protein level of Nurr1 was significantly and gradually decreased in the gerbil hippocampus with increasing age. In addition, strong Nurr1 immunoreactivity was primarily observed in pyramidal neurons and granule cells of the hippocampus in the young group, which was determined to be reduced in the adult group and to a greater extent in the aged group. Collectively the data demonstrated that Nurr1 immunoreactivity was gradually and markedly decreased during normal aging. These results indicate that gradual decrease of Nurr1 expression in the hippocampus may be associated with the normal aging process and a decline in hippocampus-dependent cognitive function.</P>

Preventing Extracellular Diffusion of Trigeminal Nitric Oxide Enhances Formalin-induced Orofacial Pain

Hwi-Seok Jung,Hong-Bin Jeon,Ik-Sung Jeon,Bum-Jun Lee,Hyun-Woo Yoo,Dong-Kuk Ahn,Dong-Ho Youn 대한생리학회-대한약리학회 2009 The Korean Journal of Physiology & Pharmacology Vol.13 No.5

Nitric oxide (NO), a diffusible gas, is produced in the central nervous system, including the spinal cord dorsal horn and the trigeminal nucleus, the first central areas processing nociceptive information from periphery. In the spinal cord, it has been demonstrated that NO acts as pronociceptive or antinociceptive mediators, apparently in a concentration-dependent manner. However, the central role of NO in the trigeminal nucleus remains uncertain in support of processing the orofacial nociception. Thus, we here investigated the central role of NO in formalin (3%)-induced orofacial pain in rats by administering membrane-permeable or -impermeable inhibitors, relating to the NO signaling pathways, into intracisternal space. The intracisternal pretreatments with the NO synthase inhibitor L-NAME, the NO-sensitive guanylate cyclase inhibitor ODQ, and the protein kinase C inhibitor GF109203X, all of which are permeable to the cell membrane, significantly reduced the formalin- induced pain, whereas the membrane-impermeable NO scavenger PTIO significantly enhanced it, compared to vehicle controls. These data suggest that an overall effect of NO production in the trigeminal nucleus is pronociceptive, but NO extracellularly diffused out of its producing neurons would have an antinociceptive action.

Long-Term Exercise Improves Memory Deficits via Restoration of Myelin and Microvessel Damage, and Enhancement of Neurogenesis in the Aged Gerbil Hippocampus After Ischemic Stroke

Ahn, Ji Hyeon,Choi, Jung Hoon,Park, Joon Ha,Kim, In Hye,Cho, Jeong-Hwi,Lee, Jae-Chul,Koo, Hyun-Mo,Hwangbo, Gak,Yoo, Ki-Yeon,Lee, Choong Hyun,Hwang, In Koo,Cho, Jun Hwi,Choi, Soo Young,Kwon, Young-Guen SAGE Publications 2016 Neurorehabilitation and neural repair Vol.30 No.9

<P>Background. The positive correlation between therapeutic exercise and memory recovery in cases of ischemia has been extensively studied; however, long-term exercise begun after ischemic neuronal death as a chronic neurorestorative strategy has not yet been thoroughly examined. Objective. The purpose of this study is to investigate possible mechanisms by which exercise ameliorates ischemia-induced memory impairment in the aged gerbil hippocampus after transient cerebral ischemia. Methods. Treadmill exercise was begun 5 days after ischemia-reperfusion (I-R) and lasted for 1 or 4 weeks. The animals were sacrificed 31 days after the induction of ischemia. Changes in short-term memory, as well as the hippocampal expression of markers of cell proliferation, neuroblast differentiation, neurogenesis, myelin and microvessel repair, and growth factors were examined by immunohistochemistry and/or western blots. Results. Four weeks of exercise facilitated memory recovery despite neuronal damage in the stratum pyramidale (SP) of the hippocampal CA1 region and in the polymorphic layer (PoL) of the dentate gyrus (DG) after I-R. Long-term exercise enhanced cell proliferation and neuroblast differentiation in a time-dependent manner, and newly generated mature cells were found in the granule cell layer of the DG, but not in the SP of the CA1 region or in the PoL of the DG. In addition, long-term exercise ameliorated ischemia-induced damage of myelin and microvessels, which was correlated with increased BDNF expression in the CA1 region and the DG. Conclusions. These results suggest that long-term treadmill exercise after I-R can restore memory function through replacement of multiple damaged structures in the ischemic aged hippocampus.</P>