http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Determination of optimal conditions for ribonucleic acid production by Candida tropicalis no. 121
Huajing Ren,Hanjie Ying,Xiaochun Chen,Jiaming Cao,Lei Li,Jianxin Bai,Yong Chen,Jian Xiong 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.8
The experiments were based on multivariate statistical concepts, and response surface methodology (RSM)was applied to optimize the fermentation medium for the production of ribonucleic acid (RNA) by Candida tropicalis no. 121. The process involved the individual adjustment and optimization of various medium components at shake flask level. The two-level Plackett-Burman (PB) design was used to screen the medium components, which significantly influenced RNA production. Among seven variables, the concentrations of molasses, ZnSO_4, and H_3PO_4 were found to be the important factors that significantly affected RNA production (confidence levels above 95%). These factors were further optimized using a central composite design (CCD) and RSM. The optimum values for the critical components were as follows: molasses 47.21 g/L: ZnSO_4 0.048 g/L; H_3PO_4 1.19 g/L. Under optimal conditions, RNA production was 2.56 g/L, which was in excellent agreement with the predicted value (2.561 g/L), and led to a 2.1-fold increase compare with that using the original medium in RNA production.