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- 저자 : HuaZhong Li (검색결과 2 건)
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Yiqun Wang,Yongpan Liu,Cong Wang,Zewei Li,Xiao Sheng,Hyung Gyu Lee,Naehyuck Chang,Huazhong Yang IEEE 2016 IEEE transactions on computer-aided design of inte Vol.35 No.2
<P>Energy harvesting from natural environment gives range of benefits for the Internet of things. Scavenging energy from photovoltaic (PV) cells is one of the most practical solutions in terms of power density among existing energy harvesting sources. PV power systems mandate the maximum power point tracking (MPPT) to scavenge the maximum possible solar energy. In general, a switching-mode power converter, an MPPT charger, controls the charging current to the energy storage element (a battery or equivalent), and the energy storage element provides power to the load device. The mismatch between the maximum power point (MPP) current and the load current is managed by the energy storage element. However, such architecture causes significant energy loss (typically over 20%) and a significant weight/volume and a high cost due to the cascaded power converters and the energy storage element. This paper pioneers a converter-less PV power system with the MPPT that directly supplies power to the load without the power converters or the energy storage element. The proposed system uses a nonvolatile microprocessor to enable an extremely fine-grain dynamic power management in a few hundred microseconds. This makes it possible to match the load current with the MPP current. We present detailed modeling, simulation, and optimization of the proposed energy harvesting system including the radio frequency transceiver. Experiments show that the proposed setup achieves an 87.1% of overall system efficiency during a day, 30.6% higher than the conventional MPPT methods in actual measurements, and thus a significantly higher duty cycle under a weak solar irradiance.</P>
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Kai Qian,XiaoHai Gong,Bo Guan,SuPing Wu,JingJing Zhang,Jing Qian,YanFei Cai,Yun Chen,ZuoYing Duan,Xin Ma,HuaZhong Li,Jian Jin 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
Glucagon-like peptide-1 (GLP-1) was a potential therapeutic drug for type II diabetes, mainly because of the stimulatory effect on insulin secretion under condition of high blood glucose. We used PCR to obtain a recombination gene, GGH, in which two GLP-1 (GLP-1A2G) mutants were connected in series and then fused to the N terminal of human serum albumin. The fusion gene was inserted into pGAPZaA plasmid with Saccharomyces cerevisiae α- factor secretion signal sequence, and was expressed by the glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. The engineered strain was constructed by integrating the recombinant plasmid pGAPZαA/GGH into the genome of Pichia pastoris GS115. Genome PCR and western blot showed that the recombinant P. pastoris successfully expressed the fusion protein GGH. The yield of GGH reached 78 mg/L after 72 h fermentation in a flask, using glucose as the optimal carbon source. Fed-batch fermentation was investigated in a 5 L bioreactor, and the expression level of GGH reached 246 mg/L in 52 h. The fusion protein GGH was purified in four steps, and the final purity was 96.1%. The in vitro bioactivity of GGH was the same as that expressed in P. pastoris by the AOX1 promoter. This study described an efficient way to express GGH fusion protein in P. pastoris using GAP promoter, fermentation was easier to control without carbon source change and fermentation time was 20 h less than AOX1 promotercontrolled GGH fermentation.
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