목재가 선적된 선창에서 발생한 산소결핍에 의한 질식사

김동훈,김기권,장태정,김정란,이구,김수근,임현술 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

Oxygen deficiency has been frequent in a clouted space. Wood consumes oxygen and discharges carbon dioxide Instead of photosynthesis in closed space without light, so do some microorganisms on the surface. We experienced a case that a healthy Insect-proofer fell down and died of asphyxia on stair-board at 7 m below the hatch of the cargo-hold shipping wood Analysis of gases in cargo-hold revealed O2; 12.3%, CO; 105 ppm, CH4; 2.7%, and H2S; 1.9% at 1 m below the hatch, and then O2; 6.1%, CO; 220 ppm, CH4; 2.9%, and H2S, 2.3 ppm at 2.5 m below the hatch. Autopsy findings were unremarkable. We justiced the cause of death asphyxia due to oxygen deficiency. As seen in this case, the serious oxygen deficiency was accounted for oxygen consumption by wood and microorganisms.

여성노인의 저항운동 시 세트구성 방법의 차이가 Myokine에 미치는 영향

김명수 ( Kim¸ Myung-soo ),이승환 ( Lee¸ Sung-hwan ),민병남 ( Min¸ Byung-nam ),김재훈 ( Kim¸ Jae-hoon1 ),방현석 ( Bang¸ Hyun-seok ),김성희 ( Kim¸ Sung-hee ) 한국융합과학회(구 한국시큐리티융합경영학회) 2020 한국융합과학회지 Vol.9 No.4

연구목적: 본 연구는 여성노인을 대상으로 저항운동 시 사용되는 set구성 방법에 중 ascending set, descending set, drop set를 각각 적용시켜 Myokine의 변화를 알아보고자 하였다. 연구방법: 연구대상은 노인복지시설을 이용하고 있는 만 65-72세 사이의 여성노인 32명을 대상으로 ascending set, 8명, descending set 8명, drop set 8명, control group 8명으로 구분하였다. 자료처리는 반복측정에 의한 이원변량분석(two-way ANOVA with repeated measure)을 사용하였으며, 유의도는 .05로 하였다. 결론: 운동프로그램은 12주간 주 3회 실시하였으며, 신체구성과 IL-15, BDNF, VEGF, Irisin의 변화를 관찰하였다. 연구결과 신체구성의 체중, 체지방률, 제지방량 모두 유의한 차이가 없었다. IL-15와 BDNF는 운동 전·후의 비교결과 Descending set와 Drop set에서 운동 후 유의하게 증가하였고, 집단 간의 차이에서 Descending set와 Drop set 집단이 대조집단에 비하여 높게 나타났다. VEGF는 운동 전·후의 비교결과 Descending set와 Drop set 집단이 유의하게 증가하였으며, 집단 간의 차이에서 Descending set와 Drop set가 Ascending set와 control group 보다 높게 나타났다. Irisin은 집단 간 차이는 없었지만 Ascending, Descending, Drop set집단에서 운동 전에 비하여 운동 후 유의한 차이로 증가하였다.. Purpose: The purpose of this study is to identify the change of Myokine by applying ascending set, descending set, and drop set which are set composition used during elderly women’s resistance training. Method: 32 participants aged 65 to 72 who were users of welfare facilities for the aged were divided into 4 groups. They were ascending set group of 8, descending set group of 8, drop set group of 8, and control group of 8. The training program was conducted 3 times a week for 12 weeks and the change of body composition, IL-15(interleukin-15), BDNF(brain derived neurotrophic factor), VEGF(Vascular endothelial growth factor) and Irisin was observed. Two-way ANOVA with repeated measure was used for data processing. Result: The result of this study is as follows. Weight, %fat, and LBM(lean body mass) without fat showed no significant change. IL-15 and BDNF increased significantly after training in Descending set group(p<.05; p<.01)and Drop set group(p<.01; p<.05). VEGF significantly increased in descending set group(p<.01) and drop set group(p<.05). Among groups, VEGF was higher in descending set and drop set groups than ascending set and control groups. Irisin showed no difference between groups, but it showed significant increase after training in ascending(p<.05), descending(p<.05), and drop set groups(p<.05).

捻轉法이 竝行된 鍼刺戟 및 電鍼刺戟이 鎭痛效果에 미치는 影響

Park, Dong-Seok,Kim, Ji-Hoon,Min, Byung-Il,Oh, Soo-Myung 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Dong-Seok Park¹, Ji-Hoon Kim¹ and Byung-Il Min, Soo-Myung Oh,³¹Department of Acupuncture and Moxibustion, College of Medicine, ²Department of Physiology, College of Medicine, And ³East-West Medical Research Institute, Kyung Hee University,Seoul, Korea. Analgesic Effect of Manual Acupuncture and Electroacupuncture combined with Twirling Method. Proceeding of International Symposium on East-West Medicine Seoul. 187-200, 1999.-Twirling the needle (TN) is one of several needing methods frequently used for acupuncture in the oriental medicine. The present study was conducted to see if TN enhanced the antinociceptive effect produced by plain-or electroacupuncture in the rat tail flick test. Rats (Sprague-Dawley, 200-300g)lightly anesthetized with thiopental sodium(40mg/kg, i.p.) were used in the tail-flick test. The basal reaction time for tail-flick was 2 sec. For "plain" acupuncture(PA), a needle was inserted for 20 min into a Chock-samni (ST36) acupuncture point which (is located at the anterior tibial muscle and about 10mm below the knee joint). For electroacupuncture(EA), train-pulses(3 Hz,0.3-ms pulse width, 0.2-0.3mA) were applied to the inserted needle for the period of insertion. For combining TN with either PA or EA, TN was performed during the each acupuncture period. Two methods for TN were used; twirling 3 times for 1 second persisted for 1 min in every 5 mins(long-duration and long-interval;LDLI) and twirling 3 times for 1 second persisted for 10 sec in every 1 min(short-duration and short-interval;SDSI). PA and EA produced an increase in tail flick latency (TFL); peak increases were 21.3±5.1% and 42.1±17.3% of the pre-acupunture control, respectively, and occurred immediately after cessation of 20min-acupuncture. Performing TN combined with PA increased TFL more than PA itself by showing a greater peak increase in TFL when performed with a SDSI-TN than with a LDLI-TN(60.6±12.5% and 42.7±22.7% of the pre-acupuncture control, respectively). Performing TN combined with EA also increased TFL more than EA itself and a greater peak increase in TFL was observed with a SDSI-TN as compared to a LDLI-TN(77.5±13.8% and 67.3±14.0% of the pre-acupuncture control, respectively). These results indicate that TN enhances both PA-and EA-produced antinociception where an greater enhanced antinociception than a LDLI-TN. It is suggested that performing a SDSI-TN combined with EA seems to be one of the most effective ways to produce analgesia.

Apoptotic Effect of Co-treatment with Chios Gum Mastic and HS-1200 on G361 Human Melanoma Cell Line

Young-Joo Hur,Young-Ki Kim,Hyun-Ho Kwak,Gyoo-Cheon Kim,Seung-Eun Lee,In-Ryoung Kim,Chul-Hoon Kim1,Bong-Soo Park 대한해부학회 2009 Anatomy & Cell Biology Vol.42 No.2

Chios gum mastic (CGM) is a resinous exudate obtained from the stem and the main leaves of Pistacia lenticulus tree native to Mediterranean areas. Recently it reported that CGM induce apoptosis in a few cancer cells in vitro. Bile acids and their synthetic derivatives induced apoptosis in various kinds of cancer cells and anticancer effects. It has been reported that the synthetic chenodeoxycholic acid (CDCA) derivatives showed apoptosis-inducing activity on various cancer cells in vitro. This study was undertaken to investigate the synergistic apoptotic effect of cotreatment with a natural product, CGM and a CDCA derivative, HS-1200 on G361 human melanoma cells. To investigate whether the co-treatment of CGM and HS-1200 compared with each single treatment efficiently reduced the viability of G361 cells, MTT assay was conducted. To investigate augmentation of apoptosis in G631 cells co-treated with CGM and HS-1200, DNA electrophoresis, Hoechst staining, proteasome activity assay, flow cytometry, Westen blot analyses, immunofluorescent staining and confocal microscopy were performed. In this study, G361 cells co-treated with CGM and HS-1200 showed several lines of apoptotic manifestation such as nuclear condensations, DNA fragmentation, the reduction of MMP and proteasome activity, the decrease of DNA content, the release of cytochrome c into cytosol, the translocation of AIF and DFF40 (CAD) onto nuclei, activation of caspase-9, caspase-3, PARP and DFF45 (ICAD), and up-regulation of Bax whereas each single treated G361 cells did not. Although the single treatment of 40 μg/mL CGM or 25 μM HS-1200 for 24 hrs did not induce apoptosis, the co-treatment of them induced prominently apoptosis. Therefore, combination therapy of CGM and HS-1200 could be considered, in the future, as an alternative therapeutic strategy for human melanoma.

재조합 Saccharomyces cerevisiae로부터 인체 리포코틴-Ⅰ의 분비 생산 및 정제

김병문,정봉현,이상기,박영훈,남수완 동의대학교 기초과학연구소 1996 基礎科學硏究論文集 Vol.6 No.1

Human lipocortin-I (LCI) is a calcium ion-dependent and phospholipid-binding protein which exhibits an anti-inflammatory activity by inhibiting phospholipase A₂ activity. In this study, the LCI gene containing its own terminator region was joined to GAL10 promoter-ppL (prepro-leader sequence of mating factor a). An ATG start codon of LCI gene was placed at downstream with KR endoprotease recognition site (Lys-Arg) of ppL. Recombinant S. cerevisiae harboring the LCI expression/secretion vector, pYGLPT5, was aerobically grown on a liquid YPDG medium at 30℃ for 72hrs. The whole cell and culture supernatant were separated after centrifugation, and the expressed LCI was analyzed by SDS-PAGE and western blotting methods. A majority fraction of the expressed LCI was found to be accumulated in the intracellular fraction, resulting in very low secretion efficiency of about 7.4%. About 500mg/ℓof LCI was extracellularly produced by the fed-batch culture employing the controlled-feeding of glucose and galactose. The secreted LCI was purified by ultrafiltration and hydroxylapatite column chromatography, and a purity of more than 99% was obtained.

CFG를 이용한 제어문 음성 인식에 관한 연구

김상범,김주성,김수훈,허강인 東亞大學校 附設 情報通信硏究所 1996 情報通信硏究所論文誌 Vol.4 No.1

In this study, we perform continuous speech recognition system using context free grammar. We use DDCHMM as train model, that is able to express time-transient-variation very well. Using one pass DP method as recognition algorithm which is attempted by expanding dynamically a finite automaton from a part of suggesting context-free-grammar of recognition process. We can increase the recognition rates of control sentence speech recognition. Car control command, robot control command, and newspapers editorial continuous speech are made to test recognition rates of control sentence speech using context-free-grammer.

Android SDK를 이용한 2D RPG 모바일 게임 제작

오경훈, 안성옥, 김수균 배재대학교 공학연구소 2012 공학논문집 Vol.14 No.1

Mobile game Soul Chaser was manufactured using the Android SDK. The game uses 2D graphics and RPG genre. Neat graphics and simple, easy-operation can be enjoyed by anyone, Based on the scenario with the completed structure, Causing the player's interest through a variety of events foreshadowing. Simple interface complex operation is difficult because of the nature of the mobile games, Story of conversational RPG game genre is very appropriate, because the With the development of the mobile gaming market will be even more prominence. KEY WORDS:Mobile game,Android SDK.

수종 수복재에 불소 도포제 적용 후 불소유리에 관한 비교연구

김수연,김종수,유승훈 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.3

韓國産 未記錄種 게類에 關하여

金熏洙 成均館大學校 1966 論文集 Vol.11 No.-

著者가 1963年부터 1966年 사이에 大蘭芝島, 濟州島의 西歸浦, 巨文島, 突山島에서 韓國 未記錄種 게類 5科 8種을 얻었다. 이로써 韓國産 게類는 總 17科 135種(亞種 包含)이 된다. 本論文에서 報告한 게類는 다음과 같다. Family Homolidae Henderson 1. Latreillia phalangium de Haan 사슴게 Family Leucosiidae Dana 2. Merocryptus lambriformis A. Milne Edwards 세모밤게 3. Leucosia haematosticta Adams & White 붉은점밤게 Family Majidae Alcock 4. Leptomithrax bifidus Ortmann 가시두두럭게 5. Acanthophrys longispinus (de Haan) 뭉툭가시뿔게 Family Portunidae Dana 6. Charybdis rivers-andersoni Alcock. 얼룩민꽃게 Family Pinnotheridae Dana 7. Pinnixa tumida Stimpson 흰해삼속사리게 Family Grapsidae Dana 8. Helice leachii Hess 남방범게 이들 8種 中에서 Latreillia phalangium, Leptomithrax bifidus, Pinnixa tumida는 極東 溫帶海域에만 分布하는 것이고 其他 5種은 熱帶 海域에도 分布하는 것이다. In the present paper 8 species of brachyuran decapods are added in the list as new to the brachyuran fauna of Korea. The materials were collected at the coastal seas of Daenanji Do, Sogwipo, Geomun Do and Dolsan Do in summers during a period ranging from 1963 to 1966. The total brachyuran decapods found in Korea up to the present time are 17 families, 135 species and subspeaies. The following list indicates 8 species mentioned above. Family Homolidae Henderson 1. Latreillia phalangium de Haan Family Leucosiidae Dana 2. Merocryptus lambriformis A. Milne Edwards 3. Leucosia haematosticta Adams & White Family Majidae Alcock 4. Leptomithrax bifidus Ortmann 5. Acanthophrys longispinus (de Haan) Family Portunidae Dana 6. Charybdis rivers-andersoni Alcock Family Pinnotheridae Dana 7. Pinnixa tumida Stimpson Family Grapsidae Dana 8. Helice leachii Hess

補中益氣湯 및 少陰人 補中益氣湯이 S-180에 對한 抗腫瘍效果와 Cyclophosphamide에 依한 副作用에 미치는 影響

金秀鎭,金聖勳,林樂哲 대한동의병리학회 1993 동의생리병리학회지 Vol.8 No.-

연구배경 : 암은 사망률 1위를 차지하고 있는 질환으로서, 치료는 수술요법, 방사선요법, 화학요법 및 면역요법 등을 주로 활용하고 있는데, 그 중에서도 활용도가 높은 항암제에 의한 화학요법은 암종에 대한 감수성 차이, 치료후의 부작용, 재발 및 합병증 등의 문제점으로 부작용을 감소시키면서 항암효과를 높이는 치료법이 요구되고 있다. 이에 암에 응용될 수 있는 한방제재를 개발하고 있는 암의 치료에 기여하고자 한다. 방법 : Sarcoma 180을 이식하여 생쥐의 생명연장율과 cytophosphamide를 다량 투여하여 부작용을 유발시킨 백서의 GOT, GPT활성도, BUN 및 creatinine 함량을 측정하였다. 결과 : 실험군에서 공히 생명연장 효과가 인정되었고, GOT, GPT활성도도 유의성이 인정되었다. 혈청중 BUN 및 creatinine 함량은 소음인보중익기탕군에서 유의성이 인정되었고, 보중익기탕군에서는 유의성은 없었으나 감소하는 경향이 있었다. 결론 : 보중익기탕 및 소음인보중익기탕은 정기를 보익하고 면역기능을 촉진시켜 암종을 억제하는 효과가 있어, 암의 한방치료 및 예방에 응용할 수 있을 것으로 사료된다. Experiment was done to study the effects of liquid extract of Bojungikgitang(BIT) & Soeuminbojungikgitang(SBT) on the antitumor activity against S-180 and the reduction of side effects induced by cyclophosphamide. The results were obtained as follows : 1. The survival rate against S180 was increased in both BIT-treated group and SBT-treated group. 2. GOT was significantly decreased in both BIT-treated group and SBT-treated group as compared with the control group. 3. GPT was significantly decreased in both BIT-treated group and SBT-treated group as compared with the control group. 4. The amount of serum BUN was significantly decreased only in SBT-treated group. 5. The amount of serum creatinine was significantly decreased only in SBT-treated group. From above results, it was thought that Bojungikgitang & Soeuminbojungikgitang had antitumor activity against s-180 and the reduction of side effects induced by cyclophosphamide.