A three-dimensional in vitro culture model for primary neonatal rat ventricular myocytes

Hongxia Zheng,Shanshan Liu,Wei-ming Tian,Hong-ji Yan,Yao Zhang,Yu Li 한국물리학회 2012 Current Applied Physics Vol.12 No.3

To overcome bad prognosis of patients with heart failure and the lack of organ donors, cardiac tissue engineering has developed as a biomimetic approach to repair, replace, and regenerate the damaged cardiac tissue. During the past decade years, researchers are devoted to find different natural and/or synthetic materials that can build appropriate physical structures to contain and organize implanted cells. In this study, we present a new method for primary neonatal rat cardiomyocytes culture in vitro using alginate/collagen/chitosan hydrogel. To investigate the feasibility of this material as scaffold for cardiac myocytes, neonatal rat ventricular myocytes were isolated and encapsulated in alginate-based beads cross-linked with calcium ion. The growth of cells was evaluated by staining with a-Sarcomeric actin (a-SCA) and Troponin T type 2 (TNNT2), and the viability of cardiomyocytes was studied in vitro by assessing the expression levels of several cardiac ion channels, including CACNL1A1, Connexin 43 and SCN5A. The results showed a significant increase in cardiac myocytes number, and the expression levels of CACNL1A1, Connexin 43 (Cx43) were up-regulated significantly except SCN5A, as compared with twodimensional cultures. Moreover, extracellular matrix produced by the seeded cells themselves was observed by staining with fibronectin. Taken together, these findings indicate that this alginate/collagen/chitosan hydrogel bead is suitable for supporting the growth and retaining the morphologic and electrophysiologic characteristics of primary cultured rat cardiac muscle cells.

Deformable Liposomes for Topical Skin Delivery of Arbutin

김대덕,Shengjie Bian,최민구,Hongxia Lin,Junmin Zheng,정석재,심창구 한국약제학회 2006 Journal of Pharmaceutical Investigation Vol.36 No.5

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Carnosic acid protects against acetaminophen-induced hepatotoxicity by potentiating Nrf2-mediated antioxidant capacity in mice

Qi Guo,Zhiyang Shen,Hongxia Yu,Gaofeng Lu,Yong Yu,Xia Liu,Pengyuan Zheng 대한생리학회-대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.1

Acetaminophen (APAP) overdose is one of the most common causes of acute liver failure. The study aimed to investigate the protective effect of carnosic acid (CA) on APAP-induced acute hepatotoxicity and its underlying mechanism in mice. To induce hepatotoxicity, APAP solution (400 mg/kg) was administered into mice by intraperitoneal injection. Histological analysis revealed that CA treatment significantly ameliorated APAP-induced hepatic necrosis. The levels of both alanine aminotransferase (ALT) and aspartate transaminase (AST) in serum were reduced by CA treatment. Moreover, CA treatment significantly inhibited APAP-induced hepatocytes necrosis and lactate dehydrogenase (LDH) releasing. Western blot analysis showed that CA abrogated APAP-induced cleaved caspase-3, Bax and phosphorylated JNK protein expression. Further results showed that CA treatment markedly inhibited APAP-induced pro-inflammatory cytokines TNF-a, IL-1b, IL-6 and MCP-1 mRNA expression and the levels of phosphorylated IkBa and p65 protein in the liver. In addition, CA treatment reduced APAP- induced hepatic malondialdehyde (MDA) contents and reactive oxygen species (ROS) accumulation. Conversely, hepatic glutathione (GSH) level was increased by administration of CA in APAP-treated mice. Mechanistically, CA facilitated Nrf2 translocation into nuclear through blocking the interaction between Nrf2 and Keap1, which, in turn, upregulated anti-oxidant genes mRNA expression. Taken together, our results indicate that CA facilitates Nrf2 nuclear translocation, causing induction of Nrf2-dependent genes, which contributes to protection from acetaminophen hepatotoxicity.

Study on crystallization kinetics and curing mechanism of the multiple heavy metals in slag glass-ceramics

Yongsheng Du,Ying Wei,Zhicheng Zheng,Yuhang Guo,Hongxia Zhang,Leibo Deng,Hua Chen,Ming Zhao 한양대학교 청정에너지연구소 2024 Journal of Ceramic Processing Research Vol.25 No.2

In this paper, manganese-containing blast furnace slag (MBFS) was used as the main raw material and Cr2O3 was used asthe nucleating agent to prepare MBFS glass-ceramic by melting method. The influence of multiple heavy metal ions on thecrystallization characteristics, physical and chemical properties of glass-ceramic was studied by adding CuO and ZnO, and theexistence of multiple heavy metal ions in glass-ceramic and its curing effect were determined. The results showed that Cr wasthe key element for crystallization of glass-ceramic, which can enable volume crystallization to occur. The glass-ceramics withdiopside as the main crystalline phase had excellent comprehensive physical and chemical properties. The results of TCLPheavy metal leaching experiments also confirmed that spinel crystal wrapped by diopside and glass phase can produce anexcellent solidification effect on multiple heavy metals.

Carnosic acid protects against acetaminophen-induced hepatotoxicity by potentiating Nrf2-mediated antioxidant capacity in mice

Guo, Qi,Shen, Zhiyang,Yu, Hongxia,Lu, Gaofeng,Yu, Yong,Liu, Xia,Zheng, Pengyuan The Korean Society of Pharmacology 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.1

Acetaminophen (APAP) overdose is one of the most common causes of acute liver failure. The study aimed to investigate the protective effect of carnosic acid (CA) on APAP-induced acute hepatotoxicity and its underlying mechanism in mice. To induce hepatotoxicity, APAP solution (400 mg/kg) was administered into mice by intraperitoneal injection. Histological analysis revealed that CA treatment significantly ameliorated APAP-induced hepatic necrosis. The levels of both alanine aminotransferase (ALT) and aspartate transaminase (AST) in serum were reduced by CA treatment. Moreover, CA treatment significantly inhibited APAP-induced hepatocytes necrosis and lactate dehydrogenase (LDH) releasing. Western blot analysis showed that CA abrogated APAP-induced cleaved caspase-3, Bax and phosphorylated JNK protein expression. Further results showed that CA treatment markedly inhibited APAP-induced pro-inflammatory cytokines TNF-${\alpha}$, IL-$1{\beta}$, IL-6 and MCP-1 mRNA expression and the levels of phosphorylated $I{\kappa}B{\alpha}$ and p65 protein in the liver. In addition, CA treatment reduced APAP- induced hepatic malondialdehyde (MDA) contents and reactive oxygen species (ROS) accumulation. Conversely, hepatic glutathione (GSH) level was increased by administration of CA in APAP-treated mice. Mechanistically, CA facilitated Nrf2 translocation into nuclear through blocking the interaction between Nrf2 and Keap1, which, in turn, upregulated anti-oxidant genes mRNA expression. Taken together, our results indicate that CA facilitates Nrf2 nuclear translocation, causing induction of Nrf2-dependent genes, which contributes to protection from acetaminophen hepatotoxicity.

Identification of Mouse MARVELD1 as a Microtu-bule Associated Protein that Inhibits Cell Cycle Progression and Migration

Fanli Zeng,Yanyan Tian,Shuliang Shi,Qiong Wu,Shanshan Liu,Hongxia Zheng,Lei Yue,Yu Li 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.3

MARVEL domain-containing 1 (MARVELD1) is a newly identified nuclear protein; however its function has not been clear until now. Here, we report that mouse MARVELD1(mMARVELD1), which is highly conserved between mice and humans, exhibits cell cycle-dependent cellular localization. In NIH3T3 cells, MARVELD1 was observed in the nucleus and at the perinuclear region during interphase,but was localized at the mitotic spindle and midbody at metaphase, and a significant fraction of mMARVELD1translocated to the plasma membrane during anaphase. In addition, treatment of cells with colchicine, a microtubuledepolymerizing agent, resulted in translocation of mMARVELD1to the plasma membrane, and association of mMARVELD1 and α-tubulin was confirmed by co-immunoprecipitation. Finally, overexpression of mMARVELD1 resulted in a remarkable inhibition of cell proliferation, G1-phase arrest, and reduced cell migration. These findings indicate that mMARVELD1 is a microtubule-associated protein that plays an important role in cell cycle progression and migration.

Identification of pepper genes involved in the response to CO 2 enrichment using RNA-Seq analysis

Jing Zhang,Rui Bai,Mengya Shang,Xiaoyong Xu,Hongxia Song,Shaowen Zheng,Leiping Hou,Meilan Li,Guoming Xing 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.1

Pepper is widely cultivated, and the application of CO 2 promotes photosynthesis and increases its yield. However, the molecularmechanisms underlying this are still unclear. In this study, the photosynthetic correlation indexes under elevated CO 2and control conditions were compared. The application of CO 2 increased the photosynthetic capacity of pepper. Moreover,RNA-Seq analysis was used to identify genes that were diff erentially expressed between pepper leaves grown in CO 2 -enrichedconditions and those grown in control conditions. The 149 diff erentially expressed genes (DEGs) were found to be involvedin photosynthesis and other metabolic processes. According to GO signifi cant enrichment analysis, the proteins encodedby the DEGs were mainly found to be located in the chloroplast, the chloroplast matrix, and the apoplast. According toKEGG signifi cant enrichment analysis, the DEGs were found to be involved in glutathione metabolism; starch and sucrosemetabolism; and stilbenoid, diarylheptanoid, gingerol, fl avonoid, and phenylpropanoid biosynthesis. The DEGs were alsoinvolved in the pentose phosphate pathway, carbon metabolism, and porphyrin and chlorophyll metabolism. Based on theGO annotation and the KEGG database analysis, ten of the DEGs identifi ed were suggested to be involved in photosynthesisand related processes; these genes were predicted to have roles in carbohydrate, soluble sugar, and glutathione metabolism,and in raffi nose, cysteine, nucleotide, and ABA biosynthesis. These DEGs are involved in the pentose phosphate pathwayand tricarboxylic acid cycle of carbon assimilation during photosynthesis. One of the DEGs was also found to be involved inchlorophyll biosynthesis. These results lay the foundation for further investigation of the molecular mechanisms and genesinvolved in the response to CO 2 enrichment in peppers.

Influence of In-Plane Simple Shear Strain on the Grain Orientation Regulation and Stretch Formability of Pre-twinned AZ31 Magnesium Alloy Sheet

Liangliang Xue,Lifei Wang,Pengbin Lu,Qiang Zhang,Hua Zhang,Guangsheng Huang,Liwei Lu,Liuwei Zheng,Hanuma Reddy Tiyyagura,Hongxia Wang,Shuyong Jiang,Kwang Seon Shin 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.10

Pre-twin is widely accepted as an efficient method for weakening the basal texture to improve the plasticity of magnesium alloys, particularly for formability. However, the enhancement is limited since the largest Schmid factor (SF) of basal slip cannot be achieved. In-plane simple shear strain is used to control the twin orientation in accordance with Schmid law to further decrease the basal texture and improve the formability of the Mg alloys sheet. Consequently, secondary regulation of initial twin orientation (SRITO) technology, a novel combination procedure, was developed. In this method, the AZ31 Mg alloys sheet is compressed to pre-twins with various volumes (1%, 3%, and 5%), then the twin orientation is regulated by in-plane simple shear strain for the second time. Compared with the as-received sample, the average fracture elongation of the annealed sample with the best properties improves from 15.3 to 28.8%, and the Erichsen value increases from 2.8 to 6.1 mm due to the regulation of twin orientation.