Research on Novel 3D Modelling Methodology for Animation Design based on Computer Graphics Theory and Mathematical Analysis with Kinect Implementation

Honglin Huang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.10

At present, the 3D object model technique is being applied in the simulation, virtual reality, 3D film industry and culture protection, and many other fields which can be widely applied. However, the existing 3D reconstruction technology is based on the complex and often expensive sensors. Therefore, we conduct research on computer graphics theory and mathematical modelling based 3D reconstruction methodology with Kinect. We firstly introduce the basic concepts and application of Kinect system which serves as the preliminary of our research. Then, we conduct mathematical modelling for our proposed system with theoretical analysis. Later, experiment and numerical simulation is conducted for verifying the robustness and effectiveness of our proposed approach. The visualized and numerical simulation result indicates that our method outperforms and holds better accuracy compared with other popular adopted algorithms. In the future, we plan to combine more optimization techniques to polish and modify our approach.

Disruption of the Myostatin Gene in Porcine Primary Fibroblasts and Embryos Using Zinc-Finger Nucleases

Huang, Xian-Ju,Zhang, Hong-Xiao,Wang, Huili,Xiong, Kai,Qin, Ling,Liu, Honglin Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.4

Myostatin represses muscle growth by negatively regulating the number and size of muscle fibers. Myostatin loss-of-function can result in the double-muscling phenotype and increased muscle mass. Thus, knockout of myostatin gene could improve the quality of meat from mammals. In the present study, zinc finger nucleases, a useful tool for generating gene knockout animals, were designed to target exon 1 of the myostatin gene. The designed ZFNs were introduced into porcine primary fibroblasts and early implantation embryos via electroporation and microinjection, respectively. Mutations around the ZFNs target site were detected in both primary fibroblasts and blastocysts. The proportion of mutant fibroblast cells and blastocyst was 4.81% and 5.31%, respectively. Thus, ZFNs can be used to knockout myostatin in porcine primary fibroblasts and early implantation embryos.

De novo genome assembly and annotation of Holothuria scabra (Jaeger, 1833) from nanopore sequencing reads

Luo Honglin,Huang Guanghua,Li Jianbin,Yang Qiong,Zhu Jiajie,Zhang Bin,Feng Pengfei,Zhang Yongde,Yang Xueming 한국유전학회 2022 Genes & Genomics Vol.44 No.12

Background: Holothuria scabra is a costly gourmet and traditional Chinese tonic medicine. However, the lack of high-quality genome information hinders the genetic, phylogenetic, and bioactivator researches. Objective: To construct high-quality genomic data of H. scabra and conduct genome-wide phylogenetic analysis. Methods: The whole genome of a male H. scabra was sequenced based on Nanopore MinION platform, and the sequence was assembled by wtdbg2. Transcriptome sequencing was used to aid the gene annotation. Repeat sequences, non-coding RNA, pseudogene and gene functional annotation were analyzed. 750 single-copy gene families from ten species were applied to construct phylogenetic tree for evolutionary analysis by using the ML method. Results: The H. scabra genome of 1.18 Gb (N50 = 1557,492 bp) with 500.42 Mb of putative repetitive sequences was assembled from a male H. scabra individual, and 16,642 protein-coding genes, 951 pseudogenes, 1791 motifs and 45,400 domains from the generated assembly were identified. The divergence time between H. scabra and its ancestor was estimated approximately 192.6 million years ago. H. scabra and A. japonicas joined together while sea urchin and sea star diverged about 440 Mya ago. Some key genes involved in notochord and gill slits development, skeleton degeneration and nervous system, as well as homeobox genes differ between H. scabra and Apostichopus japonicas. Conclusion: We report the first whole genome of H. scabra with expectation that this will be a valuable resource for genetic, phylogenetic, breading, molecular biology and bioactivator studies of sea cucumbers and other invertebrates.

Disruption of the Myostatin Gene in Porcine Primary Fibroblasts and Embryos Using Zinc-Finger Nucleases

Xian-Ju Huang,Hong-Xiao Zhang,Huili Wang,Kai Xiong,Ling Qin,Honglin Liu 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.4

Myostatin represses muscle growth by negatively regulating the number and size of muscle fibers. Myostatin loss-of-function can result in the double-muscling phenotype and increased muscle mass. Thus, knockout of myostatin gene could improve the quality of meat from mammals. In the present study, zinc finger nucleases, a useful tool for generating gene knockout animals, were designed to target exon 1 of the myostatin gene. The designed ZFNs were introduced into porcine primary fibroblasts and early implantation embryos via electroporation and microinjection, respectively. Mutations around the ZFNs target site were detected in both primary fibroblasts and blastocysts. The proportion of mutant fibroblast cells and blastocyst was 4.81% and 5.31%, respectively. Thus, ZFNs can be used to knockout myostatin in porcine primary fibroblasts and early implantation embryos.

Effective removal of phenol from wastewater by magnetic porous loofah biochar

Shan Shirui,Wu Honglin,Yang Jian,Jiao Di,Huang Mengqin,Li Fu 한국탄소학회 2024 Carbon Letters Vol.34 No.1

In this study, a low-cost and easily recyclable porous green adsorbent (magnetic porous loofah biochar, MPLB) was synthesized by modifying the almost zero-cost loofah biochar material with Fe3O4. The successful synthesis of the material was demonstrated by XRD, FTIR, SEM, VSM, and BET. In addition, the material exhibits outstanding magnetic separation performance (40.01 umg/g) allowing for rapid recovery within just 90 s. The adsorption process of phenol on MPLB was found to be spontaneous and endothermic. The experimental data fit exceptionally well with the pseudo-second-order kinetic model and Langmuir model (R2 > 0.99), indicating that the dominant adsorption mechanisms involved monolayer adsorption and chemisorption. These interactions were attributed to host–guest interaction, π–π conjugation, hydrogen bonding, and pore filling. The maximum adsorption capacity calculated using the Langmuir model at 298 K is 39.4 mg/g. Importantly, even after undergoing seven cycles of recycling, MPLB retained 78% of its initial adsorption capacity. In simulated experiments employing MPLB for phenol removal in actual wastewater, an impressive removal rate of 96.4% was achieved. In conclusion, MPLB exhibits significant potential as an effective adsorbent for phenol removal in wastewater. Graphical In this study, a low-cost and easily recyclable porous green adsorbent (magnetic porous loofah biochar, MPLB) was synthesized by modifying the almost zero-cost loofah biochar material with Fe3O4. The successful synthesis of the material was demonstrated by XRD, FTIR, SEM, VSM, and BET. In addition, the material exhibits outstanding magnetic separation performance (40.01 umg/g) allowing for rapid recovery within just 90 s. The adsorption process of phenol on MPLB was found to be spontaneous and endothermic. The experimental data fit exceptionally well with the pseudo-second-order kinetic model and Langmuir model (R2 > 0.99), indicating that the dominant adsorption mechanisms involved monolayer adsorption and chemisorption. These interactions were attributed to host–guest interaction, π–π conjugation, hydrogen bonding, and pore filling. The maximum adsorption capacity calculated using the Langmuir model at 298 K is 39.4 mg/g. Importantly, even after undergoing seven cycles of recycling, MPLB retained 78% of its initial adsorption capacity. In simulated experiments employing MPLB for phenol removal in actual wastewater, an impressive removal rate of 96.4% was achieved. In conclusion, MPLB exhibits significant potential as an effective adsorbent for phenol removal in wastewater. Graphical

In-Situ Precipitated Needle Like Nanocrystalline β-Ti Reinforced Porous Titanium Alloy via Molten Salt Electrolysis

Hao Wang,Jiahao Zhao,Honglin Li,Zhifeng Huang,Yangwei Wang,Qiang Shen,Fei Chen 대한금속·재료학회 2024 METALS AND MATERIALS International Vol.30 No.1

Fabricating porous active metals through chemical dealloying poses challenges due to their reactivity and vulnerability tooxidation in aqueous solutions. The objective of this study was to create micron-sized porous Ti alloy by utilizing the Ti–Mo system as a precursor alloy for chemical dealloying. The impact of phase composition and initial microstructure of theprecursor alloys (Tix at% Mo100 − x at%, x = 60 ~ 70) on the morphology of the resulting porous Ti alloy was systematicallyinvestigated. To improve the mechanical strength and minimize oxidized phases during the dealloying process, a molten saltelectrolysis (MSE) method was employed. The strengthening mechanism of MSE on porous Ti alloys encompassed three keyaspects. Firstly, it effectively reduced the presence of oxidized phases, thereby eliminating surface defects. Secondly, MSEfacilitated grain growth and eliminated voids and cracks at the grain boundaries, leading to enhanced mechanical properties. Thirdly, the involvement of a secondary phase contributed to the overall strengthening mechanism. Following MSE treatment,the oxygen content in the porous Ti alloy decreased from over 13 to 5 at%, and needle-like nanocrystalline β-Ti precipitatesformed within the ligament structure. The accumulation and aggregation of compression-induced dislocations at the grainboundaries of the precipitated phase further improved the mechanical properties. In summary, this work presents an innovativeapproach to fabricating porous Ti alloy with low oxygen content, high strength, and adjustable microstructure. It elucidatesthe strength enhancement mechanism by MSE, providing insights for future materials development and applications.

Static Var Generator Based on Accurate Linearization Combined with Sliding Mode Controller

Liu Fei,Wang Xueyan,Huang Xinyi,Yan Honglin,Huang Xiangdong,Pan Hongguang 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.5

The active and reactive current are hard to be decoupled completely with strong robustness when the traditional proportional integral (PI) algorithm is adopted to feedforward decoupling control for static var generator (SVG). This paper proposed a compound control strategy for SVG based on precise linearization and sliding mode control (SMC). To be specifi c, fi rst, the theory of diff erential geometry is used to prove that the mathematical model of SVG satisfi es the necessary and suffi - cient condition of precise linearization. Second, the precise linearized high-order system of SVG was transformed into two completely independent decoupled fi rst-order linear systems, and realize complete decoupling of active current and reactive current. Finally, SMC was used to design both the voltage outer loop and the current inner loop. Simulations ware carried out for the SVG mathematical model under three conditions which were inductive load, capacitive load and sudden change load. The results of those simulation showed that the controller designed by the proposed control strategy has the advantages of quick reactive power compensation, strong robustness and anti-interference performance.

Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells

Bo-jiang Li,Ping-hua Li,Rui=hua Huang,Wen-xing Sun,Han Wang,Qi-fa Li,Jie Chen,Wang Jun Wu,Honglin Liu 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.8

The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.

Three sesquiterpene lactones suppress lung adenocarcinoma by blocking TMEM16A-mediated Ca2+-activated Cl− channels

Ruilian Xiu,Jie Jia,Qing Zhang,Fengjiao Liu,Yaxin Jia,Yuanyuan Zhang,Beibei Song,Xiaodan Liu,Jingwei Chen,Dongyang Huang,Fan Zhang,Juanjuan Ma,Honglin Li,Xuan Zhang,Yunyun Geng 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.6

Transmembrane protein TMEM16A, which encodes calcium-activated chloride channel has been implicated in tumorigenesis. Overexpression of TMEM16A is associated with poor prognosis and low overall survival in multiple cancers including lung adenocarcinoma, making it a promising biomarker and therapeutic target. In this study, three structure-related sesquiterpene lactones (mecheliolide, costunolide and dehydrocostus lactone) were extracted from the traditional Chinese medicine Aucklandiae Radix and identified as novel TMEM16A inhibitors with comparable inhibitory effects. Their effects on the proliferation and migration of lung adenocarcinoma cells were examined. Whole-cell patch clamp experiments showed that these sesquiterpene lactones potently inhibited recombinant TMEM16A currents in a concentration- dependent manner. The half-maximal concentration (IC50) values for three tested sesquiterpene lactones were 29.9 ± 1.1 μM, 19.7 ± 0.4 μM, and 24.5 ± 2.1 μM, while the maximal effect (Emax) values were 100.0% ± 2.8%, 85.8% ± 0.9%, and 88.3% ± 4.6%, respectively. These sesquiterpene lactones also significantly inhibited the endogenous TMEM16A currents and proliferation, and migration of LA795 lung cancer cells. These results demonstrate that mecheliolide, costunolide and dehydrocostus lactone are novel TMEM16A inhibitors and potential candidates for lung adenocarcinoma therapy.