고분자 전지용 Polypyrrole/Sulfonated-poly(2,6-dimethyl-1,4-phenylene oxide) 복합전극의 전기화학적 특성

정홍련,김찬,양갑승,이완진 한국공업화학회 2004 응용화학 Vol.8 No.1

Polypyrrole/PPO and polypyrrole/SPPO, conducting polymer composite, were made by a oxidative chemical polymerization with changing the contents of pyrrole and PPO using FeCl₃ act as a dopant and a initiator. The electrode was made by hot press on Cu exmat after mix conducting polymer composite and Super-P, and was examined electrochemical characteristics to penetrate to 1 M LiClO₄ + PC/DMC. The charge-discharge performance of the PPy/SPPO composite electrode was increased the extent of about 50 % compared to that of PPy/PPO. The reason is that the miscibility, contact area and affinity are promoted owing to the effect of sulfonation.

태권도 옆차기 동작의 우수자와 비우수자간의 운동학적 비교 분석

이호진,한재희,김륜건,홍예은,우상연 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

The purposes of this study were to analyze the kinematic variations between skilled and unskilled individuals and obtain the data needed to improve effective side kick skill in Teakwondo. We used a video analysis program to collect performance time, changes in movement of the center of the body, angle, and angular velocity. The results were as follows : 1) in unskilled cases, side kick motion occurred for more time than in unskilled cases, 2) to make a stronger kick, the skilled group increased the joint speed of the legs and unskilled athletes have smaller angles than experienced athletes. As a result, the total performance time of side kicks was shorter for skilled people and was consistent with other previous studies. The angle of the knee joint and hip joint had a great influence on the side kick.The range of motion of the hip and knee joints should be increased so that the desired hitting point can be quickly reached.

면접조사를 통한 초등학생의 영양교육 실태 및 실천도 조사

오유진,이영미,김정현,안홍석,김정원,박혜련,서정숙,김경원,권오란,박혜경,이은주,성현이 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.4

This study investigated the experience and practice of elementary school students on nutrition education. The data were collected from 217 male and female students attending 5-6th grade elementary schools in Seoul and Kyunggi-Do from March to June 2007, interviewing face to face by a nutrition teacher and 3 interns of a nutrition teacher. The results were as follows: 86.5% of the subjects learned about ‘Table etiquette’, ‘Reasons for eating fruits and vegetables’ (78.7%), ‘Food waste and environment’ (72.3%), ‘Healthy snacks’ (55.7%), ‘Food sanitation’ (52.3%), ‘Food culture of foreign countries’ (48.1%). Nutrition education experience was significantly different by gender. A total of 43.5% boys responded that they never learned about ‘basic food preperation’ (p < 0.01). They had learned ‘Nutrients for body’ and ‘Food waste and environment’ in school, ‘Healthy weight loss’, ‘Food culture of foreign countries’, ‘Food circulation’ on television, Most content (‘Table etiquette’, ‘Simple cooking’, ‘Food sanitati n’, ‘Eating behaviors for health’, ‘Reasons for eating fruits and vegetables’, ‘Healthy snacks’) was learned from parents. The practice after nutrition education was higher in ‘Table etiquette’ (2.14), ‘Eating fruits and vegetables’ (2.07) than others compared with education experience. The most reason of non-practice on nutrition information was ‘Troublesome’. In ‘Nutrients for body’, a boy answered ‘Difficult for practice’ 20.0%, a girl answered ‘Difficult to understand’ 32.6%, showing a significant difference between the gender groups (p < 0.001). They remembered the ‘Nutrients for body’ (49.6%), ‘Food sanitation’ (44.5%) because of ‘important content’,‘Basic food preparation’ (40.6%), ‘Food culture of foreign countries’ (36.3%) because of ‘interesting content’, ‘Healthy weight loss’ (52.0%), ‘Eating behavior for health’ (44.5%) and ‘Healthy snacks’ (33.7%) because of ‘need for my health’.

Effect of Coagulants on the Performance of an Air-Cathode Microbial Fuel Cell

Hong-Suck Kim,Byung-Goon Kim,Ryun-Kyong Kim,Dae-Hyun Kim 대한환경공학회 2015 대한환경공학회 학술발표논문집 Vol.2015 No.10

First Report of Cucumber mosaic virus Infecting Pinewood Coneflower (Rudbeckia bicolor) in Korea

Kim, Mi-Kyeong,Kwak, Hae-Ryun,Ko, Sug-Ju,Lee, Su-Heon,Kim, Jeong-Soo,Kim, Kook-Hyung,Cha, Byeong-Jin,Choi, Hong-Soo The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.1

A virus isolate causing symptoms of yellow mosaic, fern leaves, malformation and plant necrosis on Rudbeckia bicolor was prevalent around Pyeongchang area in Korea. The causal virus was identified as Cucumber mosaic virus (CMV) using characteristics from biological, serological and molecular analyses and named as CMV-Rb. CMV-Rb caused mosaic on Nicotiana benthamiana, N. tabacum, Capsicum annuum, and Lycopersicon esculentum. However, typical local lesions did not develop on inoculated Pisum sativum, Cucurbita moschata, Datura stramonium and Tetragonia expansa plants. Full-length genome sequences of CMV-Rb RNAs 1, 2 and 3 were obtained using 12 primer pairs by RT-PCR analysis. The genome of CMV-Rb RNA segments 1, 2, and 3 consists of 3363nt, 3049nt, and 2214nt in length, respectively. In order to ascertain their taxonomic identity, nucleotide and the deduced amino acid sequence analyses RNAs 1, 2 and 3 of CMV-Rb isolates were conducted with previously reported sequences of CMV strains and/or isolates. CMV-Rb RNAs showed about 90 to 99% sequence identity to those of subgroup I strains suggesting that CMV-Rb is more closely related to CMV isolates belong to subgroup I. To our knowledge, this is the first report of CMV on Rudbeckia bicolor in Korea.

HNRNPA1, a Splicing Regulator, Is an Effective Target Protein for Cervical Cancer Detection: Comparison With Conventional Tumor Markers

Kim, Young-Jon,Kim, Byoung-Ryun,Ryu, Jae-Suk,Lee, Gyeong-Ok,Kim, Hak-Ryul,Choi, Keum-Ha,Ryu, Jae-Won,Na, Kyoung-Suk,Park, Min-Cheol,So, Hong-Seob,Cho, Ji-Hyun,Park, Do-Sim Blackwell Scientific Publications 2017 International journal of gynecological cancer Vol.27 No.2

<B>Objective</B><P>Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), serine/arginine-rich splicing factor 1 (SRSF1), and SRSF3 are splicing regulators associated with oncogenesis. However, the alterations of SF proteins and their diagnostic values in cervical cancer are unclear. To apply SFs clinically, effective marker selection and characterization of the target organ properties are essential.</P><B>Materials and Methods</B><P>We concurrently analyzed HNRNPA1, SRSF1, SRSF3, and the conventional tumor markers squamous cell carcinoma antigen (SCCA) and carcinoembryonic antigen (CEA) in cervical tissue samples (n = 127) using semiquantitative immunoblotting. In addition, we compared them with p16 (cyclin-dependent kinase inhibitor 2A [CDKN2A]), which has shown high diagnostic efficacy in immunohistochemical staining studies and has been proposed as a candidate protein for point-of-care screening biochemical tests of cervical neoplasia.</P><B>Results</B><P>HNRNPA1, higher molecular weight forms of SRSF1 (SRSF1-HMws), SRSF3, CEA, and p16 levels were higher (<I>P</I> < 0.05) in cervical carcinoma tissue samples than in nontumoral cervical tissue samples. However, the levels of SRSF1-Total (sum of SRSF1-HMws and a lower molecular weight form of SRSF1) and SCCA, a commonly used cervical tumor marker, were not different between carcinoma and nontumoral tissue samples. In paired sample comparisons, HNRNPA1 (94%) showed the highest incidence of up-regulation (carcinoma/nontumor, >1.5) in cervical carcinoma, followed by p16 (84%), SRSF1-HMws (69%), SRSF3 (66%), CEA (66 %), SCCA (32%), and SRSF1-Total (31%). HNRNPA1 (92%) and p16 (91%) presented the two highest diagnostic accuracies for cervical carcinoma, which were superior to those of SRSF3 (75%), SRSF1-HMws (72%), CEA (72%), SCCA (59%), and SRSF1-Total (55%).</P><B>Conclusions</B><P>Our results identified that HNRNPA1 is the best diagnostic marker among the SFs and conventional markers given its excellent diagnostic efficacy for cervical carcinoma, and it has a p16-comparable diagnostic value. We suggest that HNRNPA1 is an additional effective target protein for developing cervical cancer detection tools.</P>

소장종양의 임상적 고찰

김해경,김병식,김진천,홍원선,남승우,민영일,정훈용,김해련,하현권,양석균,이재균,최재원,오성태 대한소화기학회 1998 대한소화기학회지 Vol.31 No.1

Background/Aims: Small bowel tumors are uncommon accounting for only 3 to 6% of all gastrointestinal tumors. The diagnosis of small bowel tumors has been usually made at an advanced stage because of the infrequency and absence of symptoms specific for early diagnosis, resulting in poor prognosis. This study was conducted to investigate the clinical features of small bowel tumors in Korea. Methods: We reviewed and analyzed clinical manifestations, laboratory results and survivals in 63 patients with small bowel tumors diagnosed at the Asan Medical Center between June 1989 and January 1996. Results: Among 63 tuors reviewed, 58 were malignant tumors and 5 were benign tumors. The malignant tumors consisted of 25 adenocarcinomas, 15 malignant lymphomas, 12 leiomyosarcomas and 6 metastatic carcinomas. Adenocarcinomas were located mainly in the duodenum and malignant lymphomas in the ileum or duodenum, while leiomyosarcomas were evenly distributed. Clinical symptoms of these patients included abdominal pain (71.4%), palpable mass (12.7%), weight loss (10.0%) and bleeding (8.0%). Anemia and positive stool occult blood test were found in 55.0% and 46.7%, respectively. The median duration of symptoms before the diagnosis was 4 weeks in malignant tumors and 36 weeks in benign tumors. Twenty-seven of 30 patients (90.0%) with duodenal tumors were diagnosed by duodenoscopy, while small bowel series and CT scans detected 9 of 11 (82.0%) and 25 of 35 (71.4%) small bowel tumors, respectively. The incidence of metastasis to regional lymph nodes and distant organs was higher in adenocarcinoma and malignant lymphoma than leiomyosarcoma. The 2-year survival rates for patients with adenocarcinomas, malignant lymphomas, and leiomyosarcomas were 23, 57, and 60%, respectively. Conclusions: These results suggest that small bowel tumors should be considered in patients having unexplained abdominal pain, anemia, and/or positive stool occult blood test.

Simultaneous determination of the levels of deoxynivalenol, 3-acetyldeoxynivalenol, and nivalenol in grain and feed samples from South Korea using a high-performance liquid chromatography-photodiode array detector

Kim, Dong-Ho,Hong, Sung-Yong,Jeon, Mi-Hyeon,An, Jae-Min,Kim, Sung-Youn,Kim, Hyo-Young,Yoon, Bo Ryun,Chung, Soo Hyun The Korean Society for Applied Biological Chemistr 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.6

An HPLC method combined with immunoaffinity clean-up was developed for the simultaneous analysis of deoxynivalenol (DON), nivalenol (NIV), and 3-acetyldeoxynivalenol (3AcDON) in grains and feeds. The limits of quantification of the method for DON, 3AcDON, and NIV were 11.0, 27.6, and 13.5 ng/g, respectively. HPLC analyses of 300 samples of grains and feeds collected in South Korea showed that the incidence of DON and NIV in corn was 22.5 and 7.5%, while that in rice was 4.0 and 54.0%, respectively. The incidence of DON and NIV in mixed grain powder was 62.0 and 24.0%, respectively. Our study also showed that animal feeds were mainly contaminated with DON (98.1%) in the range of 32.8-950.25 ng/g with the mean concentration of 353.32 ng/g. The levels of DON in grains did not exceed the maximum allowable limit (1 mg/kg) set by the Korean Food and Drug Administration.

Time-evolving genetic networks reveal a NAC troika that negatively regulates leaf senescence in <i>Arabidopsis</i>

Kim, Hyo Jung,Park, Ji-Hwan,Kim, Jingil,Kim, Jung Ju,Hong, Sunghyun,Kim, Jeongsik,Kim, Jin Hee,Woo, Hye Ryun,Hyeon, Changbong,Lim, Pyung Ok,Nam, Hong Gil,Hwang, Daehee National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.21

<▼1><P><B>Significance</B></P><P>Leaf senescence is regulated in a complex manner, involving time-dependent interactions with developmental and environmental signals. Genetic screens have identified key regulators of senescence, particularly late-stage senescence regulators. Recently, time-course gene-expression and network analyses, mostly analyses of static networks, have predicted many senescence regulators. However, senescence is defined by time-evolving networks, involving the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks of NAM/ATAF/CUC (NAC) transcription factors, central regulators of leaf senescence in <I>Arabidopsis</I>, via time-course gene-expression analysis of NACs in their mutants. These time-evolving networks revealed a unique regulatory module of NACs that controls the timely induction of senescence-promoting processes at a presenescent stage of leaf aging.</P></▼1><▼2><P>Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in <I>Arabidopsis</I> during leaf aging. The most evident characteristic of these time-dependent networks was a shift from positive to negative regulation among NACs at a presenescent stage. ANAC017, ANAC082, and ANAC090, referred to as a “NAC troika,” govern the positive-to-negative regulatory shift. Knockout of the NAC troika accelerated senescence and the induction of other <I>NAC</I>s, whereas overexpression of the NAC troika had the opposite effects. Transcriptome and molecular analyses revealed shared suppression of senescence-promoting processes by the NAC troika, including salicylic acid (SA) and reactive oxygen species (ROS) responses, but with predominant regulation of SA and ROS responses by ANAC090 and ANAC017, respectively. Our time-evolving networks provide a unique regulatory module of presenescent repressors that direct the timely induction of senescence-promoting processes at the presenescent stage of leaf aging.</P></▼2>

Efficacy and Safety of FOLFIRI Regimen in Elderly Versus Nonelderly Patients with Metastatic Colorectal or Gastric Cancer

Kim, Ji‐,Won,Lee, Keun‐,Wook,Kim, Kyu‐,Pyo,Lee, Ju Hyun,Hong, Yong Sang,Kim, Jeong‐,Eun,Kim, Sun Young,Park, Sook Ryun,Nam, Byung‐,Ho,Cho, Sang‐,Hee,Chung, Ikȁ AlphaMed Press 2017 The oncologist Vol.22 No.3

<P>Background. Irinotecan-based chemotherapy is a standard backbone of therapy in patients with metastatic colorectal cancer (CRC) or gastric cancer (GC). However, there is still a paucity of information concerning the efficacy and safety of irinotecanbased regimens in elderly patients. Patients and Methods. Using the patient cohort (n=1,545) from the UGT1A1 genotype study, we compared the efficacy and safety between elderly and nonelderly patients with metastatic CRC (n=934) or GC (n=611) who received first-or second-line FOLFIRI (irinotecan, leucovorin, and 5-fluorouracil) chemotherapy. Results. Despite lower relative dose intensity in elderly patients, progression-free survival and overall survival were similar between elderly (age >= 70 years) and nonelderly (<70 years) patients in the CRC cohort (hazard ratio [HR], 1.117; 95% confidence interval [CI], 0.927-1.345; p=.244, and HR, 0.989; 95% CI, 0.774-1.264; p=.931, respectively) and the GC cohort (HR, 1.093; 95% CI, 0.854-1.400; p=.479, and HR, 1.188; 95% CI, 0.891-1.585; p=.241, respectively). In both cohorts, febrile neutropenia (22.1% vs. 14.6% in CRC cohort and 35.2% vs. 22.5% in GC cohort) and asthenia (grade 3: 8.4% vs. 1.7% in CRC cohort and 5.5% vs. 2.9% in GC cohort) were more frequent in elderly patients. In the CRC cohort, mucositis and anorexia were more frequent in elderly patients. In the GC cohort, nausea and vomiting were less frequent in elderly patients. Conclusion. The efficacy of the FOLFIRI regimen was similar between elderly and nonelderly patients in both the CRC and the GC cohorts. However, special attention should be paid to elderly patients because of increased risk for febrile neutropenia and asthenia.</P>