당뇨병 환자의 혈청 Lipoprotein에 관한 연구

홍영숙,성낙응,경난호,이순남 梨花女子大學校 醫科大學 醫科學硏究所 1984 EMJ (Ewha medical journal) Vol.7 No.3

Lipoprotein cholesterol and triglyceride levels have been determined innormal and diabetes. The abnormalities of lipoprotein were investigated by SDS-polyacrylamide gel electrophoresis in the serum and the 3 major lipoprotein classes for diabetes. HDL cholesterol levels were lower in the diabetes compared to the normals. VLDL triglyceride levels also elevated in the diabetes. There were significant shifts in the distribution of lipoprotein cholesterol, with an increase in LDL cholesterol and decrease in HDL cholesterol. The results of this study suggest that diabetes may be associated with changes in both lipoprotein triglyceride and cholesterol levels. The electrohoretic patterns of VLDL and HDL in diabetes showed the abnorm-al pattern in comparison to normal.

Genetic Diversity and Population Genetic Structure of Cephalotaxus koreana in South Korea

Hong, Kyung Nak,Kim, Young Mi,Park, Yu Jin,Lee, Jei Wan The Plant Resources Society of Korea 2014 한국자원식물학회지 Vol.27 No.6

The Korean plum yew (Cephalotaxus koreana Nakai) is a shade-tolerant, coniferous shrub. The seeds have been used as a folk medicine in Korea, and an alkaloid extract (HTT) is known to have anticancer properties. We estimated the genetic diversity of 429 trees in 16 populations in South Korea using 194 polymorphic amplicons from seven combinations of AFLP primer-restriction enzymes. The average number of effective alleles and the percentage of polymorphic loci were 1.37 and 79.4%, respectively. Shannon's diversity index and the expected heterozygosity were 0.344 and 0.244, respectively. We divided 16 populations into four groups on the UPGMA dendrogram and the PCA biplot. The first two principal components explained 84% of the total genetic variation. Genetic differentiation between populations explained 14% of total genetic variation, and the remaining 86% came from difference between individuals within populations, as determined by an analysis of molecular variance (AMOVA). However, the genetic differentiation did not correlate with the geographic distance between populations from the Mantel test. The Bayesian statistics, which are comparable to Wright's $F_{ST}$ and Nei's $G_{ST}$, were ${\theta}^I=0.406$ and ${\theta}^{II}=0.172$, respectively. The population genetic diversity was slightly lower, and the strength of genetic differentiation was much weaker, than the average of those plants having similar life histories, as assessed using arbitrary marker systems. We discuss strategies for the genetic conservation of the plum yew in Korea.

점봉산 (點鳳山) 잣나무임분의 개체목 공간분포에 따른 유전구조

홍경락(Kyung Nak Hong),권영진(Young Jin Kwon),정재민(Jae Min Chung),신창호(Chang Ho Shin),홍용표(Yong Pyo Hong),강범룡(Bum Yong Kang) 한국산림과학회 2001 한국산림과학회지 Vol.90 No.1

Genetic differentiation of populations is resulted from the environmental and the genetic effects, and the interactions between them. Whereas, the major factors influencing to the genetic differentiation within populations are the gene flow induced by seed or pollen dispersial, the microsite heterogeneity, and the density-dependent distribution of individuals. For the purpose of studying spatial genetic structure and the distribution pattern of Korean pines(Pinus koraiensis), we set up one 100 × 100㎡ plot at a Korean pine stand in Quercus mongolica community on Mt. Jumbong in Korea. To estimate the coefficient of spatial autocorrelation as Moran`s index and an analogue, simple block distance, isozyme markers were analyzed in 325 Korean pines. For 11 polymorphic loci observed in 9 enzyme systems, the average percentage of polymorphic loci, the observed and expected heterozygocity were 72.2% 0.200, and 0.251, respectively. It was revealed the excess of homozygotes was observed in the plot, which suggests that here may be more number of consanguineous trees than expected. On the basis of isozyme genotypes observed in this study, 325 trees were classified into 147 groups in which the maximum number of trees for one group was 34. From the distance class of 24-32m, the genetic heterogeneity began to increase. The variation of simple block distance against the growth performance by tree height and diameter also showed the same trend at 24-32m class. According to high fixation index(F=0.204), the spatial genetic structure within a stand, the analysis of the growth performance, and the distribution patterns of identical genotypes, we inferred that the genetic structure of a Korean pine stand in Mt. Jumbong has been maintained rather density-dependent mechanism than the gene flow, such as the pollen dispersial or the heavy input of seeds following the forest gaps. The genetic patchy size was determined between 24-32m, which suggests that the selection of individuals for the ex situ conservation of Korean pine in Mt. Jumbong may be desirable to be made with the spatial distance over 37 meters between trees.

AFLP 표지자에서 나타난 전라남도 부안의 북방한계지에자생하는 호랑가시나무 군락의 유전적 단형성

홍경낙 ( Kyung Nak Hong ),박유진 ( Yu Jin Park ),이제완 ( Jei Wan Lee ),김영미 ( Young Mi Kim ) 한국산림과학회 2015 한국산림과학회지 Vol.104 No.2

Individuals in the population under a particular environmental condition influencing recurrently for a long time could locally adapted and local adaptation is of a fundamental importance in a breeding program, conservation activities of genetic resources or evolutionary biology. Plants at northern range limits have higher probability of expressing an adaptative genetic trait. The natural community at the northern range limit of Ilex cornuta (Chinese holly) in Buan, Jeollanam-do in Korea was composed of adults of 744 and seedlings of 211 (hereafter ‘Community’) and is designated as the Korean Natural Monument (No. 122) by the law. At two adjacent areas to Community, 85 (hereafter ‘Plantation I’) and 27 hollies (hereafter ‘Plantation II’) were planted respectively for preparations of the next generation. Eighty-five trees were sampled for genetic analysis in the three groups. Fifty-two (36%) of the total 143 amplicons were polymorphic from four AFLP primer combinations. A total of thirteen genotypes was identified and just one genotype was for 52 trees of Community. Seven and five genotypes were shown for Plantation I and II, respectively. There was no identical genotype between Community and Plantation (I or II) or between two plantation groups. Number of private loci was 2 for Community, 6 for Plantation I and 4 for Plantation II. We presumed their genetic backgrounds were quite different with one another and the plantation groups were made independently because they were different not only the genetic compositions but also their ages. Considering the genetic monomorphism by AFLP markers, observations of only male trees and asexual propagation as layerage or cuttage, the hollies in Community might be a genet by root suckering from a single male tree, not the results of selective removal of female trees for ornamental use in the past.

19 년생 테다소나무의 조림지에서 경사가 반형매가계의 생장에 미치는 영향

홍경낙(Kyung Nak Hong),김영중(Yong Jung Kim),조경진(Kyung Jin Cho),김만조(Maha Jo Kim),김용률(Young Yul Kim),서강욱(Gang Uk Suh) 한국산림과학회 2002 한국산림과학회지 Vol.91 No.4

N/A

2분과 : 오대산 물황철나무(Populus koreana) 집단의 유전다양성 및 공간적 유전구조 분석

신수경 ( Soo Kyung Shin ),송정호 ( Jeong Ho Song ),임효인 ( Hyo In Lim ),장경환 ( Kyung Hwan Jang ),홍경낙 ( Kyung Nak Hong ),이제완 ( Jei Wan Lee ) 한국임학회 2014 한국산림과학회지 Vol.103 No.1

본 연구에서는 물황철나무(Populus koreana Rehder) 집단을 대상으로 I-SSR 표지자를 이용해 유전다양성과유전적 공간구조를 분석하였다. 물황철나무는 중국, 러시아 극동지역과 북한의 고산 계곡부 등에 서식하는 낙엽활엽교목이다. 물황철나무는 남한에서 강원도 일대에 제한적으로 분포한다. 특히 오대산 집단은 물황철나무의 남방한계지로서 유전자원보존의 중요성이 강조된다. 8개의 I-SSR primer로 유전다양성을 추정한 결과, Shannon의 다양성 지수(I)는 0.230, 이형접합도의 기대치(He)는 0.151로 유사한 생활사를 갖는 타 수종에 비해 유전다양성이 매우 낮게 나타났다. 유전적 군락을 확인하기 위해 공간적 자기상관성 분석을 수행한 결과, 조사 지역 내의 물황철나무 집단은400 m 이내에서 유전적으로 유사한 군락구조를 갖고 있는 것으로 나타났다. 물황철나무 집단의 현지외 유전자 보존을 위한 표본추출 시, 개체 간 거리를 400 m 이상으로 두는 것이 효율적일 것으로 판단된다. This study describes analysis of genetic diversity and spatial genetic structure of Korean poplar(Populus koreana Rehder) in Mt. Odae using I-SSR markers. P. koreana is a deciduous broad-leaved tree species that primarily grows in the alpine valleys of China, Russia and North Korea. In South Korea, P. koreanais found limitedly in Gangwon province. Especially, the population in Mt. Odae is located on the southern limitline, its importance is emphasized from the genetic resource conservation perspective. The Shannon`s diversity(I=0.230) and the expected heterozygosity (He=0.151) were relatively low as compared with those of other plantspecies. Spatial autocorrelation analysis using Tanimoto`s distance showed that the genetic patch was founded within 400 m. It is suggested that individual trees for ex situ conservation should be sampled with a minimumdistance of 400 m between trees.

Diversity of I - SSR and RAPD Variants in Gingko biloba L . Planted in 6 Regions of Korea

(Yong Pyo Hong),(Kyung Jin Cho),(Kyung Nak Hong),(Eun Myoung Shin) 한국임학회 2000 한국임학회 정기학술발표논문집 Vol.2000 No.-

백합나무 시험림의 모의간벌 (模擬間伐)에 따른 유전다양성 변화

홍용표(Yong Pyo Hong),류근옥(Keun Ok Ryu),조경진(Kyung Jin Cho),홍경락(Kyung Nak Hong) 한국산림과학회 2001 한국산림과학회지 Vol.90 No.1

Changes in genetic diversity after seed-tree practice were simulated in test plantation of tulip tree at Sokdal-ri, Banwol-myon, Hwasung-gun, Kyungki Provence. I-SSR markers were analyzed in a total of 305 tulip trees. A total of 89 amplicon variants were observed by PCR with 9 I-SSR primers. Genetic diversity for 305 trees was relatively high (S. I. =0.4532). Individuals originated from the seed orchards in U.S.A. showed the highest level of genetic diversity (0.4530), those from Anyang showed the medium level (0.4152), and those from Cheonbuk showed the lowest (0.3929). Simulation of seed-tree practice accompanied by 2 consecutive thinnings was performed on the basis of morphological characteristics and planted distances of the individual trees, which left 37 trees as candidates for seed-trees. Decreasing rate of genetic diversity within seed sources was greatest for individuals from Cheonbuk (28.3%), moderate for those from Anyang (16.3%), and smallest for those from U.S.A. (8.0%). In spite of little difference in decreasing rate of individuals for the 3 seed sources (87.5-88.2%), large difference in decreasing rate of genetic diversity within seed sources might be due to difference in number of mother trees for the 3 seed sources. For example, whereas individuals originated from the seed orchard in U.S.A., which showed the smallest decreasing rate of genetic diversity, might be originated from relatively large number of mother trees, those from Anyang and Cheonbuk might be originated from relatively small number of mother trees. Although mean of 17.5% of the genetic diversity within seed sources was decreased through 2 consecutive thinnings, a decrease in genetic diversity for the whole individuals (37 trees) was only 6.1%. This observation suggests that the seed-tree practice on the basis of the criteria established in the present study may not result in great reduction in overall genetic diversity of the progenies.

I-SSR 표지자 분석에 의한 아까시나무 국내 집단과 신규 도입품종의 유전다양성 평가

홍용표 ( Yong Pyo Hong ),이경준 ( Kyung Joon Lee ),홍경낙 ( Kyung Nak Hong ) 한국산림과학회 2002 한국산림과학회지 Vol.91 No.6

오일산 전나물림의 숲틈에서 발생된 전나무 치수(稚樹)들의 공간적 유전구조

홍용표,최영철,강범용,홍경낙 한국임학회 2001 한국산림과학회지 Vol.90 No.4

The spatial genetic structure of Needle fir(Abies holophylla Max.) seedlings on forest gap within a Needle fir forest at Mt. Odae in Korea was analyzed on the basis of ISSR(inter-simple sequence repeats) marker analysis. The gap size was 1,500㎡(50m×30m), and we sampled 416 one- or two-year-old seedlings by 2m intervals. Some trees at the upper crown layer except Needle firs and all trees at the middle and lower crown layers were removed, and Needle firs at the upper crown layer showed very weak growth strength or to be withering to death. The results of spatial autocorrelation using 31 polymorphic ISSR markers revealed that it was genetically homogeneous within spatial distance of 15.6m and the randomness of genetic distribution was from 15.6m to 31.2m. The genetic patch size of seedlings in forest gap might be restricted by the density of mother trees, making allow for the average height of adult Needle firs, the seed dispersal area, and the average distance between adults. For the directionality of seedling distribution, we investigated the variography using 'genetic configuration' which was the value of configuration in Multidimensional Scaling by genetic distance. In directional variogram, the increment of spatial distance from East to West direction was inversely proportional to genetic homogeneity. We presumed that this anisotrophy of seedling distribution at this forest gap resulted from the directionality of seed dispersal rather than the difference of fecundity between mother trees or the microhabitat variation, taking the evenness of forest floor condition, a vast seed production and the random distribution of seedlings at the studied site into consideration.