N-acetyl cysteine inhibits H2O2-mediated reduction in the mineralization of MC3T3-E1 cells by down-regulating Nrf2/HO-1 pathway

( Daewoo Lee ),( Sung Ho Kook ),( Hyeok Ji ),( Seung Ah Lee ),( Ki Choon Choi ),( Kyung Yeol Lee ),( Jeong Chae Lee ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.11

There are controversial findings regarding the roles of nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway on bone metabolism under oxidative stress. We investigated how Nrf2/HO-1 pathway affects osteoblast differentiation of MC3T3-E1 cells in response to hydrogen peroxide (H2O2), N-acetyl cysteine (NAC), or both. Exposing the cells to H2O2 decreased the alkaline phosphatase activity, calcium accumulation, and expression of osteoblast markers, such as osteocalcin and runt-related transcription factor-2. In contrast, H2O2 treatment increased the expression of Nrf2 and HO-1 in the cells. Treatment with hemin, a chemical HO-1 inducer, mimicked the inhibitory effect of H2O2 on osteoblast differentiation by increasing the HO-1 expression and decreasing the osteogenic marker genes. Pretreatment with NAC restored all changes induced by H2O2 to near normal levels in the cells. Collectively, our findings suggest that H2O2-mediated activation of Nrf2/HO-1 pathway negatively regulates the osteoblast differentiation, which is inhibited by NAC. [BMB Reports 2015; 48(11): 636-641]

Involvement of Nrf2-Mediated Upregulation of Heme Oxygenase-1 in Mollugin-Induced Growth Inhibition and Apoptosis in Human Oral Cancer Cells

Lee, Young-Man,Auh, Q-Schick,Lee, Deok-Won,Kim, Jun-Yeol,Jung, Ha-Jin,Lee, Seung-Ho,Kim, Eun-Cheol Hindawi Publishing Corporation 2013 BioMed research international Vol.2013 No.-

<P>Although previous studies have shown that mollugin, a bioactive phytochemical isolated from <I>Rubia cordifolia</I> L. (Rubiaceae), exhibits antitumor effects, its biological activity in oral cancer has not been reported. We thus investigated the effects and putative mechanism of apoptosis induced by mollugin in human oral squamous cell carcinoma cells (OSCCs). Results show that mollugin induces cell death in a dose-dependent manner in primary and metastatic OSCCs. Mollugin-induced cell death involved apoptosis, characterized by the appearance of nuclear shrinkage, flow cytometric analysis of sub-G<SUB>1</SUB> phase arrest, and annexin V-FITC and propidium iodide staining. Western blot analysis and RT-PCR revealed that mollugin suppressed activation of NF-<I><I>κ</I></I>B and NF-<I><I>κ</I></I>B-dependent gene products involved in antiapoptosis (Bcl-2 and Bcl-xl), invasion (MMP-9 and ICAM-1), and angiogenesis (FGF-2 and VEGF). Furthermore, mollugin induced the activation of p38, ERK, and JNK and the expression of heme oxygenase-1 (HO-1) and nuclear factor E2–related factor 2 (Nrf2). Mollugin-induced growth inhibition and apoptosis of HO-1 were reversed by an HO-1 inhibitor and Nrf2 siRNA. Collectively, this is the first report to demonstrate the effectiveness of mollugin as a candidate for a chemotherapeutic agent in OSCCs via the upregulation of the HO-1 and Nrf2 pathways and the downregulation of NF-<I><I>κ</I></I>B.</P>

문자매체와 영상매체의 장르전환

최호열 청주대학교 인문과학연구소 2017 人文科學論集 Vol.54 No.-

Dans l’adaptation d’un roman pour le cinéma, le plus important, ce serait de garder la continuité entre les originals et les adaptés, et en même temps de créer la nouveauté. Dans cette étude, nous voulons essayer de trouver cette contradiction qu’on peut trouver au cours de l’adaptation, en comparant Les pluies saisonnières de Younhengil au film adapté de ce roman par KBS Media; et aussi le conte La poule qui sort de la cour de Whangseunmi au film d’animation adapté de ce conte. D’abord nous avons constaté que les oeuvres littérales ont l’avantage de la description psychologique des personnages; en réalité le cinéma aurait plus de difficulté pour bien poursuivre le cours psychologique des personnages avec des images. Par contre les oeuvres en image ont l’avantage de montrer plus facilement les événements par des images, et d'intéresser plus directement les spectateurs aux intrigues. En fait, le film d’animation a changé de l’atmosphère philosophique du conte en introduisant de nouveaux caractères et surtout le concours des canards. Enfin nous avons trouvé qu’il faut considérer avant l’adaptation, pour bien réussir au ‘storytelling’, ce genre des avantages et des désavantages.

교수법 개선을 위한 설문 조사 연구

최호열 청주대학교 학술연구소 2005 淸大學術論集 Vol.3 No.S

We said that the field of education is far behind in comparison with the other fields, economy, culture, etc, in Korea. Therefore, we often advocated the fundamental reform in education to keep up with the other fields. But we should not overlook that in modem Korea history the driving force of economic growth is originated in education. Today, the intense aspiration toward education is known all over the world and the quantitative increase in educational institutes are surpassing the advanced country. The problems that we face are converting the quantitative to quality in education. At this point of time, the universities in Korea are fortunately established a e-Learning Center and invested heavily in education. To sum up this treatise, we find that the students have a preference over classes that is progressed by means of beneficial and interesting content based on professor's own experience. More than anything else. this teaching method is effectively transmitted to the students in contrast to the classes utilizing the high-technology multimedia instrument. Consequently, we think that this high-tech multimedia instrument is not compulsory but subsidiary in teaching the students. Finally, this treatise is not shirked one's responsibility by the judgement as partial and shallow survey. But this is at least useful to the development of teaching method as a fundamental material. Also, we expect significantly that this treatise provides momentum for the thorough study in this field.

RFLP 법을 이용한 종에 따른 鹿茸類 한약재의 유전자 감식 연구

崔湖榮,鄭裕憲,鄭宗云,金圭烈 대한본초학회 2000 大韓本草學會誌 Vol.15 No.2

Cornu Cervi Pantoctricchum is a hairy, dried, non-ossific young horn of a stag on Cervus nippon Temminck or C. elaphus L (family Cervidae) and a popular crude drug used in Korea to reinforce the kidney-yang, to tonify the vital essence and blood, and to strengthen the bones and muscles. The present study is designed to make a DNA marker for breed identification of Cornu Cervi Pantotricshum families and later to establish a gene discrimination. In this experiment some genomic DNA were extracted fine from dried samples, most of them were extracted damaged a lot. In RFLP analysis using restriction enzyme Hinf I, Hae Ⅲ, Hha I and Msp I can be used to distinguish the breed of Cornu Cervi Pantotrichum. Therefore, these polymorphic bands by RFLP can be used in breed identification of deer and Cervi Cornu Pantoctrichum now in use.

사상체질과 발자크의 인물유형

최호열 청주대학교 인문과학연구소 2015 人文科學論集 Vol.51 No.-

Louis Corman a fait la morphopsychologie en adoptant la théorie de 4 liquides de Hyppocrate et celle de Jung. Et nous avons comparés ces théories avec celle de Sasang constitution de Lee Jema, en cherchant les exemples dans les personnages balzaciens. Les Dilatés extrêmes, qui se marquent par le développement physique et la tendance psychologique extravertie, sont semblables aux Soyangin et Taeyangin, qui aiment des échanges abondants et une facilité d’adaptation, et ils acceptent le monde tel qu’il est. et leur vie instinctive se déploie sans entrave; ils sont gourmands comme l’abbé Biroteau, et sensuels comme Hulot d’Evry. Ils n’ont guère d’inhibition en présence des autres et disent facilement ce qu’ils ont à dire. Au contraire, les Rétractés extrêmes sont semblables aux Soeumin et Taeeumin. Ils montrent la réctraction physique, recroquevillés sur eux-mêmes comme la cousine Bette et Trouvert. Leur vie instinctive ne s’épanouit pas aisément. Ils ne sont pas des hommes d’intuition, mais des logiciens. Ils ont une tendance psycholgique introvertie. Nous espérons que cette étude morphopsychologique aidera de comprendre mieux des hommes.

통신망 통합시뮬레이터의 구축방안에 관한 연구

권호열,김화종 강원대학교 산업기술연구소 1992 産業技術硏究 Vol.12 No.-

We proposed an integrated simulator scheme for communication networks. The network modeling and Analysis methologies are firstly discussed along the design procedure of a network simulator, and then, the structure, kernel, and user interfaces are defined for the simulator. Finally, for the verification of our approach, simulations are performed for a simple queueing network and a pure ALOHA system using Ptolemy, a multi-paradigm simulation enviroment

웹사이트 구축이 기업의 국제화에 미치는 영향

방호열,박영훈 釜山 大學校 經營 經濟 硏究所 2001 經營 經濟 硏究 Vol.20 No.1

기업이 국제화하는데 있어서 가장 큰 장애요인은 보유자원과 능력이 부족하다는 것이다. 기업이 국제화를 수행하기 위해서는 상당한 재무적·인적·마케팅 자원이 필요한데, 이러한 자원의 부족은 기업의 국제화를 어렵게 한다. 자원과 능력의 부족 외에도 기업이 국제경영활동을 수행하는데 장애가 되는 요인들로는 심리적 거리, 실무적 문제, 시장리스크 등을 들 수 있다. 이러한 장애요인을 극복하는데 있어서 효과적인 방법의 하나로 대두되고 있는 것이 인터넷을 통한 전자상거래이다. 급속한 정보통신기술의 발달과 함께 인터넷의 급속한 성장과 확산은 기업들이 국제화를 보다 원활히 수행할 수 있게 한다. 인터넷은 시간과 공간의 제약을 극복하게 해줌으로써, 규모가 작고 자원이 부족한 중소기업들도 국제화 정도를 높일 수 있는 기회를 제공한다. 본 연구는 기업이 수출장애요인을 극복하기 위한 수단으로 웹사이트구축에 중점을 두어 수출장애요인이 웹사이트구축정도에 어떠한 영향을 미치는 가를 알아보고, 이러한 웹사이트구축정도가 기업의 국제화에 어떠한 영향을 미쳤는지를 분석하고자 한다.

SSE-CMM 과 ISO/IEC 12207 의 IT보안감리 프로세스 비교

권호열 강원대학교 정보통신연구소 2004 정보통신논문지 Vol.8 No.-

This paper presents IT secunty audtmg process model based on I S O 1 ~ framework First. we discussed several IT security auditing models including ISO/IEC 12207, SPICE, GMITS, BS5799. and SSE-CMM. And we studied for a typical IT system architecture, i.e., Web-based Information System And a process model of System Security Engineering - Capability Maturity Model (SSE-CMM). Then, we compared the ISO/IEC 12207 process framework and SSE-CMM processes Finally, we suggested an improved SSE-CMM process model based on the mapping table of IS0 122M In conclusion, some research topics that related to this paper are presented for further study.

국가문화와 기업문화의 차이가 소요지분과 통제시스템에 미치는 영향

방호열,전선영 釜山 大學校 經營 經濟 硏究所 2002 經營 經濟 硏究 Vol.21 No.1

국제경영활동을 수행하고 있는 기업들이 현지국에서 직면하는 수많은 어려움은 현지문화를 정확히 이해하지 못하는데서 비롯되는 경우가 많다. 사실 각 국가들은 나름대로의 독특한 문화를 가지고 있으면서도 다른 문화와의 접촉을 통해 그 성격이 다양하고 복잡해졌기 때문에 기업이 수많은 나라의 다양한 문화에 효율적으로 대응한다는 것은 매우 어려운 일이라고 할 수 있다. 본 논문은 해외사장에 투자하여 해외자회사를 운영하고 있는 우리 나라의 제조기업을 대상으로 본사의 기업문화와 해외자회사의 국가문화적 차이를 분석하고 이를 바탕으로 해외자회사의 경영활동에 어떻게 영향을 미치는 분석할 것이다. 이를 보다 구체적으로 보면, 첫째, 소유지분을 결정하는 요인 중의 하나로써 문화적 차이가 어떤 영향을 미치는지 파악하고, 둘째, 문화적 차이가 저원가전략과 차별화전략과 같은 국제경쟁전략에 미치는 영향, 셋째, 투입통제, 행위통제, 산출통제과 같은 통제시스템에 문화적 차이가 어떠한 영향을 미치는지 선행연구를 바탕으로 분석할 것이다. 본 연구의 실증분석의 결과를 정리하면, 본사와 해외자회사의 문화적 차이가 작을수록 소유지분이 커질 것이라는 가설을 기각되었지만, 투자규모와 적당한 합작투자 파트너의 존재유무가가 소유지분을 결정하는데 중요한 요인인 것으로 나타났다.