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- 저자 : He Kanglai (검색결과 2 건)
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Jing Dapeng,Zhang Tiantao,Bai Shuxiong,He Kanglai,Prabu Sivaprasath,Wang Zhenying 한국곤충학회 2021 Entomological Research Vol.51 No.3
Yellow peach moth, Conogethes punctiferalis(Guenée) (Lepidoptera: Crambidae), is a very destructive fruit borer in Eastern Asia and Australia. A successful artificial diet and rearing protocol are urgently needed for its biological control and research. In this study, we developed and assessed four artificial diets: AD-1, AD-2, AD-3 and AD-4, for rearing yellow peach moth larvae. AD-1 is effective supplements, leading to the larval survival rate of 82.05%, percentage of pupation 79.48%, mean pupa weight of 76.53 mg, emergence rate of 95.25%, and an adult fecundity rate of 121 eggs/female. Using it to feed can shorten the whole generation developmental period to 33 days. Based on this diet AD-1, we also develop a suitable mass rearing method. During the larval stages providing three- to four-time fresh diet can keep a healthy larval colony.
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Lin Maung Kyaw,Jing Dapeng,Zhang Tiantao,Prabu Sivaprasath,He Kanglai,Bai Shuxiong,Wang Zhenying 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1
Niemann-Pick type C2 (NPC2) proteins in arthropods have been extensively differentiated and possibly dupli cated according to environmental conditions and are probable to have different functions. The participation of NPC2 proteins in chemical communication in arthropods brings new objectives in environmental-friendly stra tegies for pest population control. In this study, NPC2 gene in Macrocentrus cingulum (McinNPC2) was newly identified by rapid amplification cDNA ends (RACE) technology. McinNPC2 amino acid sequence alignment with other representative NPC2 annotates to evaluate the highly conserved consensus amino acids, but with odorant binding proteins in M. cingulum show that only one consensus amino acid. Primary six-cysteine structures that are same to odorant binding proteins in M. cingulum were observed in McinNPC2. Phylogenetic analysis of McinNPC2 indicated that the nearest monophyletic group forming one clade with high posterior probability values clusters as Cyphomyrmex costatus (CcosNPC2) whereas the nearest evolutionary relation group as some odorant binding proteins. Moreover, quantitative real-time PCR (qPCR) measurements show that the McinNPC2 gene expression level in various tissues of the female is significantly and ubiquitously higher than in male, whereas the highest expression level in female antennae. We further explore the binding characterization of recombinant McinNPC2 to candidate odor molecules and did the modeling and docking simulations. The results showed ligands binding specificity and docking tests results indicate that β-ionone, an aroma compound commonly found in essential oils, can strongly bind with McinNPC2. In conclusion, we proposed that McinNPC2 may be involved in chemical communication and play roles in perception of plant volatiles.
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