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      • SCIESCOPUSKCI등재

        Effects of Strains of Saccharomyces cerevisiae and Incubation Conditions on the In vitro Degradability of Yeast and Roughage

        Ando, S.,Nishiguchi, Y.,Hayasaka, K.,Yoshihara, Y.,Takahashi, J.,Iefuji, H. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.3

        The in vitro degradability of yeast and the effect of yeast on the in vitro degradability of forage may differ in terms of the specific yeast strains or their incubation conditions. Thus in experiment 1, two strains of sake yeast (strainK7 and strainK9) and one strain of bakers' yeast (KY5649) were incubated in an aerobic condition. In experiment 2, aerobically or anaero bically incubated K7 was used for investigating the in vitro degradability of yeast, the effect of yeast on the in vitro degradability of forage, and the degradability of yeast by pepsin and pronase treatment. The in vitrodegradability of bakers' yeast was significantly (p<0.05) higher than those of sake yeasts. The in vitro degradability of anaerobically incubated yeast was significantly (p<0.01) higher than that of aerobically incubated yeast. The degradability of bakers' yeast by pepsin treatment was significantly (p<0.01) higher than that of the sake yeasts. The degradability of bakers' yeast by pronase treatment was slightly higher than that of the two sake yeasts, while the degradability of anaerobically incubated yeast by both enzymes, respectively, was significantly (p<0.01) higher than that of aerobically incubated yeast. The degradability of forages was increased significantly (p<0.05) by the addition of yeasts. The degradability of roughage by sake yeast tended to be higher than that by the bakers' yeast. The degradability of roughage was significantly (p<0.05) higher by anaerobically incubated yeast than by aerobically incubated yeast. Given the above results, it seems that in vitro degradability of yeast and the magnitude of the increment of roughage degradation differ among the yeast strains and their incubation conditions.

      • Search for lepton-flavor and lepton-number-violating τ→@?hh<sup>'</sup> decay modes

        Belle Collaboration,Miyazaki, Y.,Hayasaka, K.,Adachi, I.,Aihara, H.,Asner, D.M.,Aulchenko, V.,Aushev, T.,Bakich, A.M.,Bay, A.,Bhardwaj, V.,Bhuyan, B.,Bischofberger, M.,Bozek, A.,Bracko, M.,Browder, T. North-Holland Pub. Co 2013 Physics letters: B Vol.719 No.4

        We search for lepton-flavor and lepton-number-violating τ decays into a lepton (@?= electron or muon) and two charged mesons (h,h<SUP>'</SUP>=π<SUP>+/-</SUP> or K<SUP>+/-</SUP>) using 854 fb<SUP>-1</SUP> of data collected with the Belle detector at the KEKB asymmetric-energy e<SUP>+</SUP>e<SUP>-</SUP> collider. We obtain 90% confidence level upper limits on the τ→@?hh<SUP>'</SUP> branching fractions in the range (2.0-8.4)x10<SUP>-8</SUP>. These results improve upon our previously published upper limits by factors of about 1.8 on average.

      • Search for τ→eγ decay at Belle

        Hayasaka, K.,Abe, K.,Abe, K.,Aihara, H.,Asano, Y.,Aushev, T.,Bahinipati, S.,Bakich, A.M.,Bedny, I.,Bitenc, U.,Bizjak, I.,Blyth, S.,Bondar, A.,Bozek, A.,Brač,ko, M.,Brodzicka, J.,Browder, T.E.,Cha Elsevier 2005 Physics letters: B Vol.613 No.1

        <P><B>Abstract</B></P><P>We have searched for the lepton-flavor-violating decay τ→eγ using a data sample of 86.7 fb<SUP>−1</SUP> collected with the Belle detector at the KEKB asymmetric <SUP>e+</SUP><SUP>e−</SUP> collider. No evidence for a signal is obtained, and we set an upper limit for the branching fraction B(τ→eγ)<3.9×<SUP>10−7</SUP> at the 90% C.L.</P>

      • SCIESCOPUSKCI등재

        Lactation Persistency as a Component Trait of the Selection Index and Increase in Reliability by Using Single Nucleotide Polymorphism in Net Merit Defined as the First Five Lactation Milk Yields and Herd Life

        Togashi, K.,Hagiya, K.,Osawa, T.,Nakanishi, T.,Yamazaki, T.,Nagamine, Y.,Lin, C.Y.,Matsumoto, S.,Aihara, M.,Hayasaka, K. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.8

        We first sought to clarify the effects of discounted rate, survival rate, and lactation persistency as a component trait of the selection index on net merit, defined as the first five lactation milks and herd life (HL) weighted by 1 and 0.389 (currently used in Japan), respectively, in units of genetic standard deviation. Survival rate increased the relative economic importance of later lactation traits and the first five lactation milk yields during the first 120 months from the start of the breeding scheme. In contrast, reliabilities of the estimated breeding value (EBV) in later lactation traits are lower than those of earlier lactation traits. We then sought to clarify the effects of applying single nucleotide polymorphism (SNP) on net merit to improve the reliability of EBV of later lactation traits to maximize their increased economic importance due to increase in survival rate. Net merit, selection accuracy, and HL increased by adding lactation persistency to the selection index whose component traits were only milk yields. Lactation persistency of the second and (especially) third parities contributed to increasing HL while maintaining the first five lactation milk yields compared with the selection index whose only component traits were milk yields. A selection index comprising the first three lactation milk yields and persistency accounted for 99.4% of net merit derived from a selection index whose components were identical to those for net merit. We consider that the selection index comprising the first three lactation milk yields and persistency is a practical method for increasing lifetime milk yield in the absence of data regarding HL. Applying SNP to the second- and third-lactation traits and HL increased net merit and HL by maximizing the increased economic importance of later lactation traits, reducing the effect of first-lactation milk yield on HL (genetic correlation ($r_G$) = -0.006), and by augmenting the effects of the second- and third-lactation milk yields on HL ($r_G$ = 0.118 and 0.257, respectively).

      • Search for lepton-flavor-violating A decays into three leptons with 719 million produced A<sup>+</sup>A<sup> </sup>pairs

        Belle Collaboration,Hayasaka, K.,Inami, K.,Miyazaki, Y.,Arinstein, K.,Aulchenko, V.,Aushev, T.,Bakich, A.M.,Bay, A.,Belous, K.,Bhardwaj, V.,Bischofberger, M.,Bozek, A.,Brako, M.,Browder, T.E.,Chang, M North-Holland Pub. Co 2010 Physics letters: B Vol.687 No.2

        <P>We present a search for lepton-flavor-violating tau decays into three leptons (electrons or muons) using 782 fb(-1) of data collected with the Belle detector at the KEKB asymmetric-energy e(+)e(-) collider. No evidence for these decays is observed and we set 90% confidence level upper limits on the branching fractions between 1.5 x 10(-8) and 2.7 x 10(-8). (C) 2010 Elsevier B.V. All rights reserved.</P>

      • Eoarchean-Paleoproterozoic zircon inheritance in Japanese Permo-Triassic granites (Unazuki area, Hida Metamorphic Complex): Unearthing more old crust and identifying source terrranes

        Horie, K.,Yamashita, M.,Hayasaka, Y.,Katoh, Y.,Tsutsumi, Y.,Katsube, A.,Hidaka, H.,Kim, H.,Cho, M. Elsevier Scientific Pub. Co., etc.] 2010 Precambrian research Vol.183 No.1

        U-Pb zircon geochronology of two Permo-Triassic granites (samples OT-52 and OT-272 with ages of 229+/-8Ma and 256+/-2Ma, respectively) in the Unazuki area, Hida Metamorphic Belt, southwest Japan, revealed the presence of Eoarchean to Paleoproterozoic inheritance. Inheritance is consistent with both samples showing low zircon saturation temperatures for their bulk compositions. In OT-52, dark in CL, low Th/U zircon domains have a mean <SUP>207</SUP>Pb/<SUP>206</SUP>Pb age of 1940+/-17Ma, which is consistent with an age of 1937+/-6Ma for anatexis in the Precambrian Busan gneiss complex in Korea. Eoarchaean inherited zircons with <SUP>207</SUP>Pb/<SUP>206</SUP>Pb ages from ca. 3750 to 3550Ma are common in OT-272 but are few in OT-52, suggesting a source from rocks with affinities to those in the Anshan area in the northeast China part of the North China Craton. On the other hand, a Hida Metamorphic Belt metasedimentary gneiss into which the granites were intruded contains ca. 1840, 1130, 580, 360, 285 and 250Ma zircons (Sano et al., 2000). These ages suggest that the Unazuki Mesozoic granites did not originate from proximal Hida Metamorphic Complex rocks, but instead from unrelated rocks obscured at depth. The predominance of Eoarchean to Paleoproterozoic age components, and the marked lack of 900-700Ma components suggest that the source was the (extended?) fringe of the North China Craton, rather than from Yangtze Craton crust. The Mesozoic evolution of Japan and its linkages to northeast Asia are discussed in the context of these results.

      • SCIESCOPUSKCI등재

        Effects of Candida utilis Treatment on the Nutrient Value of Rice Bran and the Effect of Candida utilis on the Degradation of Forages In vitro

        Ando, Sada,Nishiguchi, Y.,Hayasaka, K.,Iefuji, H.,Takahashi, J. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.6

        Candida utilis can assimilate fatty acids, so it was hypothesized that the treatment of rice by Candida utilis would improve feed quality by reducing fat content and adding the yeast function that would stimulate rumen microbes. In this study, the oil assimilation ability of Candida utilis IFO1086, 0988, 0626 and the effect of treatment of Candida utilis IFO1086, IFO0626 on the nutrient contents of rice bran were examined. The effect of Candida utilis addition on the in vitro degradability of forage was also investigated. It was found that the oil assimilating ability of IFO1086 and IFO0626 was significantly (p<0.01) higher than that of IFO0988. Candida utilis treatment reduced the EE content and increased the CP, ADF and NDF percentage. The absolute amount of ether extract was decreased by 35.9% in IFO1086 and IFO0626 treatment. The absolute amount of crude protein was not changed by yeast treatment. The ADF and NDF amounts were increased. The addition of Candida utilis increased in vitro forage degradability significantly (p<0.05). Based on these results it can be postulated that treatment of rice bran by Candida utilis may improve feed quality by reducing fat content, increasing the CP content and adding the function of yeast for stimulating rumen microbes.

      • SCIESCOPUSKCI등재

        Manipulation of Rumen Fermentation by Yeast: The Effects of Dried Beer Yeast on the In vitro Degradability of Forages and Methane Production

        Ando, S.,Khan, R.I.,Takahasi, J.,Gamo, Y.,Morikawa, R.,Nishiguchi, Y.,Hayasaka, K. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.1

        The effects of the addition of yeast on in vitro roughage degradability and methane production were investigated in order to clarify the effects of yeast on the rumen microbes and to establish methods of rumen manipulation. Three roughages (whole crop corn, rice straw and Italian ryegrass) were incubated for 3, 6, 12 and 24 h with or without dried beer yeast following the method described by Tilley and Terry. Using the same method, these roughages were incubated with or without yeast extract, albumin or purified DNA. In vitro methane production was measured with or without dried beer yeast at 12 and 24 h. The degradability of yeast was found to be 57 and 80% at 12 and 24 h, respectively. The rate of degradation of fraction b was 6.16%/h. There was a significant increase in roughage degradability at 6 h (p<0.05), 12 h (p<0.05) and 24 h (p<0.01) by dried yeast addition. The degradability of all three roughages was higher in the samples treated with yeast extract than in the no addition samples except in the case of rice straw incubated for 12 h. Nevertheless, the magnitude of increment was smaller with the addition of yeast extract than without the addition of yeast. With the addition of purified DNA, there were significant increases in roughage degradability at 6 h (p<0.01), 12 h (p<0.01) and 24 h (p<0.05); however, higher degradability values were detected in the samples to which albumin was added, particularly at 6 h. If the degradability values of the no addition samples with those of samples containing yeast, yeast extract, DNA and albumin were compared, the largest difference was found in the samples to which yeast was added, although it is worth noting that higher values were observed in the yeast extract samples than in the DNA or albumin samples, with the exception of the case of rice straw incubated for 24 h. Methane production was significantly increased at both 12 and 24 h incubation. The increment of roughage degradation and methane production brought about by the addition of dried beer yeast to the samples was thought to be due to the activation of rumen microbes. Water soluble fraction of yeast also seemed to play a role in ruminal microbe activation. The increment of degradability is thought to be partially due to the addition of crude protein or nucleic acid but it is expected that other factors play a greater role. And those factors may responsible for the different effects of individual yeast on ruminal microbes.

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