Hormonal Requirements Induced Different Regeneration Pathways in Alhagi graecorum

Hassanein, A.M. The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.3

Hormonal requirements inducing different regeneration pathways with particular emphasis on somatic embryo-genesis in Alhagi graecorum were studied. While combination of 0.5 $\mu{M}$ 2,4-dichlorophenoxyacetic acid (2,4-D), 2.5 $\mu{M}$ 6-benzylaminopurine (BAP) and 5 $\mu{M}$ 1-naphthaleneacetic acid (NAA) in MS medium induced callus formation and callus maintenance from internodal explants, each alone or in combination with other induced distinct regeneration pathway. Adventitious bud formation was induced on MS medium supplemented with 2.5 $\mu{M}$ BAP. It was improved when 2.5 $\mu{M}$ BAP was used in combination with 5 $\mu{M}$ NAA. MS medium containing 0.5 $\mu{M}$ 2,4-D or 5 $\mu{M}$ NAA induced the formation of abnormal direct somatic embryos. While increase of 2,4-D concentration (1.125-9) resulted in the formation of viable embryogenic mass, increase of NAA did not change its effect. NAA should be used in combination with 2,4-D even at low concentration (0.5 $\mu{M}$) to form embryogenic mass. In A. gaecorum, the role of 2,4-D as trigger of somatic embryogenesis and BAP as trigger of adventitious bud formation was deduced, but for maximum yield certain auxin-cytokinin ratio should be applied. Embryogenic masses characterized by high water content, low peroxidase activity, and low number of peroxidase and glutamate oxaloacetate transaminase bands in comparison with calli obtained under conditions stimulating adventitious bud formation. The resulted differential gene expression, which could be detected by native-PAGE patterns, could be used as marker for organogenic pathway in A. graecorum.

Interaction Between time of Nodal Explant Collection and Growth Regulators Determines the Efficiency of Morus alba Micropropagation

Hassanein A.M.,Galal A.A.,Azooz M.M. The Korean Society of Plant Biotechnology 2003 Plant molecular biology and biotechnology research Vol.5 No.4

The hormonal requirement suiting micropropagation of Morus alba during any season throughout the year was studied. Sprouting frequency from axillary buds of M. alba was greatly influenced by the time of explant collection, the highest value was achieved when nodal explants were collected at the end of bud dormancy period (late in March) and cultured on Murashige and Skoog (MS) medium supplemented with low concentration (0.5 mg/L) of BAP, kinetin or IBA (85-68%). In addition, they showed higher axillary bud sprouting on growth-regulators-free medium (49%) than others collected in autumn or winter and cultured on medium supplemented with various growth regulators (47-48%). Regardless of that period, young explants with greenish buds collected in summer exhibiting high sprouting frequency (66%) on MS medium supplemented with 0.5 mg/L kinetin and 0.5 mg/L GA3. Shoot multiplication via adventitious bud formation was achieved when the nodal explants were cultured on MS medium supplemented with 2 mg/L BAP and 0.2 mg/L IBA. Further multiplication via nodal explants of in vitro grown shoots was obtained on MS medium supplemented with 0.5 mglL BAP and 0.5 mg/L GA3. While half strength MS medium supplemented with low concentration (0.5 mg/L) of IBA, IAA or 2,4-D stimulated adventitious root formation, IBA was the best. After transfer the plantlets to the soil, acclimatization for three weeks was essential prerequisite for survival in high frequency (92%). Peroxidase activity is related to break of bud dormancy where maximum enzyme activity was detected when the lateral buds were induced to commence growth under field condition (early in spring) or in vitro.

Apocynin abrogates methotrexate-induced nephrotoxicity: role of TLR4/NF-κB-p65/p38-MAPK, IL-6/STAT-3, PPAR-γ, and SIRT1/FOXO3 signaling pathways

Emad H. M. Hassanein,Ahmed M. Sayed,Omnia A. M. Abd El-Ghafar,Zainab M. M. Omar,Eman K. Rashwan,Zuhair M. Mohammedsaleh,So Young Kyung,Jae Hyeon Park,Hyung Sik Kim,Fares E. M. Ali 대한약학회 2023 Archives of Pharmacal Research Vol.46 No.4

The present study was designed to evaluate the potential renoprotective impacts of apocynin (APC) against nephrotoxicity induced by methotrexate (MTX) administration. To fulfill this aim, rats were allocated into four groups: control; APC (100 mg/kg/day; orally); MTX (20 mg/kg; single intraperitoneal dose at the end of the 5th day of the experiment); and APC +MTX (APC was given orally for 5 days before and 5 days after induction of renal toxicity by MTX). On the 11th day, samples were collected to estimate kidney function biomarkers, oxidative stress, pro-inflammatory cytokines, and other molecular targets. Compared to the MTX control group, treatment with APC significantly decreased urea, creatinine, and KIM-1 levels and improved kidney histological alterations. Furthermore, APC restored oxidant/antioxidant balance, as evidenced by a remarkable alleviation of MDA, GSH, SOD, and MPO levels. Additionally, the iNOS, NO, p-NF-κB-p65, Ace-NF-κB-p65, TLR4, p-p38-MAPK, p-JAK1, and p-STAT-3 expressions were reduced, while the IκBα, PPAR-γ, SIRT1, and FOXO3 expressions were significantly increased. In NRK-52E cells, MTX-induced cytotoxicity was protected by APC in a concentration-dependent manner. In addition, increased expression of p-STAT-3 and p-JAK1/2 levels were reduced in MTX-treated NRK-52E cells by APC. The in vitro experiments revealed that APC-protected MTX-mediated renal tubular epithelial cells were damaged by inhibiting the JAK/STAT3 pathway. Besides, our in vivo and in vitro results were confirmed by predicting computational pharmacology results using molecular docking and network pharmacology analysis. In conclusion, our findings proved that APC could be a good candidate for MTX-induced renal damage due to its strong antioxidative and anti-inflammatory bioactivities.

Micro-propagation Factors Essential for Mass Production of Synthetic Seeds in Banana

Hassanein A. M.,Ibrahiem I. A.,Galal A. A.,Salem J. M. M. The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.3

This work described some essential factors necessary for micro-propagation of banana for mass production of synthetic seeds for germ plasm conservation, and how peroxides activity of conserved tissue was influenced. Shoot tips of field grown plants were used to obtain shoot clusters on shoot proliferation medium (MS medium supplemented with 5 mg/l BAP). Using longitudinally-split shoot tip technique, 18720, 8640, 7488, 2016 plantlets were obtained from one shoot tip of Maghraby, Grand Naine, Balady, and Williams, respectively, in six subculture, one month each, on solid medium. Shoot tips excised from in vitro grown plantlets were encapsulated in calcium-alginate beads and stored at $4^{\circ}C$ for one month on half-strength MS basal medium without growth regulators or sugars. After one month all the viable-conserved synseeds formed shoots when they were transferred to MS basal medium, some of them showed synchronous formation of shoot and root systems in one week. Plants retrieved from encapsulated shoot tips were hardened off and transferred to soil.

Synthesis and Biological Evaluation of Novel Imidazolone Derivatives as Potential COX-2 Inhibitors

Hassanein, Hassanein H.,Maha, M. Khalifa,El-Samaloty, Ola N.,El-Rahim, Mohamed Abd,Taha, Ragia A,Magda, M.F. Ismail 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.5

Three novel series of 2-(substituted phenyl)-4-(substituted arylidene)-imidazolone-5-(4H)-ones were derived from the corresponding oxazolones by condensation with different arylamines. Eleven of the synthesized compounds were selected and evaluated for their effect on carrageenan-induced rat paw edema. Compound 4b had the same efficacy as the reference standard (indomethacin), and compounds 3b, 3c, 4a, 4d and 9a showed good to excellent activities, with other compounds only weakly active. The potent compounds were evaluated for their inhibitory activities against COX-2-catalyzed $PGE_2$ production, with 4a, 4b and 3c showing strong inhibitory activity.

Posttraumatic Acute Disseminated Encephalomyelitis in a Child Resolved by Steroid Terapy: Case Report

Sahar M.A. Hassanein,Yosra Abdelzaher Ibrahim 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.2

Comparison of Heat Transfer in Both the Riser and Downcomer of a Circulating Fluidized Bed

Soubhi A. Hassanein,O. M. Dahab 한국연소학회 2004 KOSCOSYMPOSIUM논문집 Vol.- No.-

Safety and Efcacy of Cerebrolysin in Infants with Communication Defects due to Severe Perinatal Brain Insult: A Randomized Controlled Clinical Trial

Sahar M.A. Hassanein,Shaymaa M. Deifalla,Moustafa El-Houssinie,Somaia A. Mokbel 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.1

Background and Purpose Te neuroregenerative drug Cerebrolysin has demonstrated efcacy in improving cognition in adults with stroke and Alzheimer’s disease. Te aim of this study was to determine the efcacy and safety of Cerebrolysin in the treatment of communication defects in infants with severe perinatal brain insult. Methods A randomized placebo-controlled clinical trial was conducted in which 158 infants (age 6–21 months) with communication defects due to severe perinatal brain insult were enrolled; 120 infants completed the study. The Cerebrolysin group (n=60) received twice-weekly Cerebrolysin injections of 0.1 mL/kg body weight for 5 weeks (total of ten injections). Te placebo group (n=60) received the same amount and number of normal saline injections. Results Te baseline Communication and Symbolic-Behavior-Scale-Developmental Profle scores were comparable between the two groups. Afer 3 months, the placebo group exhibited improvements in the social (p<0.01) and speech composite (p=0.02) scores, with 10% and 1.5% increases from baseline, respectively. Te scores of the Cerebrolysin group changed from concern to no concern, with increases of 65.44%, 45.54%, 358.06%, and 96.00% from baseline in the social (p<0.001), speech (p<0.001), symbolic (p<0.001), and total (p<0.001) scores. Conclusions Cerebrolysin dramatically improved infants’ communication especially symbolic behavior which positively afected social interaction. Tese fndings suggest that cerebrolysin may be an efective and feasible way equivalent to stem cell therapy.

Human Umbilical Cord Blood CD34-Positive Cells as Predictors of the Incidence and Short-Term Outcome of Neonatal Hypoxic-Ischemic Encephalopathy: A Pilot Study

Sahar M.A. Hassanein,Mohamed Hassan Nasr Eldin,Hanaa A. Amer,Adel E. Abdelhamid,Moustafa El-Houssinie,Abir Ibrahim 대한신경과학회 2017 Journal of Clinical Neurology Vol.13 No.1

Background and Purpose Neonatal hypoxic-ischemic encephalopathy (HIE) is one of the leading causes of neurological handicap in developing countries. Human umbilical cord blood (hUCB) CD34-positive (CD34+) stem cells exhibit the potential for neural repair. We tested the hypothesis that hUCB CD34+ stem cells and other cell types [leukocytes and nucleated red blood cells (NRBCs)] that are up-regulated during the acute stage of perinatal asphyxia (PA) could play a role in the early prediction of the occurrence, severity, and mortality of HIE. Methods This case-control pilot study investigated consecutive neonates exposed to PA. The hUCB CD34+ cell count in mononuclear layers was assayed using a flow cytometer. Twenty full-term neonates with PA and 25 healthy neonates were enrolled in the study. Results The absolute CD34+ cell count (p=0.02) and the relative CD34+ cell count (CD34+%) (p<0.001) in hUCB were higher in the HIE patients (n=20) than the healthy controls. The hUCB absolute CD34+ cell count (p=0.04), CD34+% (p<0.01), and Hobel risk scores (p=0.04) were higher in patients with moderate-to-severe HIE (n=9) than in those with mild HIE (n=11). The absolute CD34+ cell count was strongly correlated with CD34+% (p<0.001), Hobel risk score (p=0.04), total leukocyte count (TLC) (p<0.001), and NRBC count (p=0.01). CD34+% was correlated with TLC (p=0.02). Conclusions hUCB CD34+ cells can be used to predict the occurrence, severity, and mortality of neonatal HIE after PA.

Hydrostatic pressure and magnetic field effect on the excited states in inverse parabolic quantum dot

S.A. Safwan,Assma Saleh,Hekmat M. Hassanein,Nagwa El Meshed 한국물리학회 2018 Current Applied Physics Vol.18 No.1

The hydrostatic pressure (P) influence of the degenerate energy states inside an inverse parabolic quantum dot (IPQD), with and without an external magnetic field, was performed within the frame of the effective mass approximation. Our theoretical results showed that the effect of relatively high pressure clearly appeared to induce a crossing between the excited states in the strong confinement region. But in the weak confinement region, such crossing disappeared and, in addition, the excited states got reordered. In the presence of an external magnetic field the hydrostatic pressure modified the crossing points of the degenerate states. We investigated the electron-heavy hole transition energy. It displayed a blue shift with increasing the pressure values and the magnetic field strength. But it showed an adhesive red shift by increasing the IPQD size.