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- 저자 : Haruka Yagi (검색결과 1 건)
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Taira Hidaka,Hiroshi Tsuno,Haruka Yagi,Yusuke Kosaka 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.2
Batch and semi-continuous thermophilic L-lactate fermentation experiments were performed using Bacillus coagulans and glucose as a substrate. Reactor performance and biomass concentrations were assessed using two methods: turbidity as a traditional biomass index and realtime polymerase chain reaction (PCR) quantification of 16S rRNA genes. In the batch experiment, although the relationship between turbidity and real-time PCR assay differed depending on the growth phase, a correlation was observed between both assay methods. In the semicontinuous experiment, real-time PCR measurement was well suited for use as an index for evaluating bacterial mass under different organic loading conditions. A mathematical model was applied to evaluate the real-time PCR quantification to long-term, semi-continuous lactate fermentation. Lactate fermentation was well suited since only B. coagulans was involved in the reactions. The results obtained revealed a fundamental relationship between real-time PCR and traditional biomass analyses. Batch and semi-continuous thermophilic L-lactate fermentation experiments were performed using Bacillus coagulans and glucose as a substrate. Reactor performance and biomass concentrations were assessed using two methods: turbidity as a traditional biomass index and realtime polymerase chain reaction (PCR) quantification of 16S rRNA genes. In the batch experiment, although the relationship between turbidity and real-time PCR assay differed depending on the growth phase, a correlation was observed between both assay methods. In the semicontinuous experiment, real-time PCR measurement was well suited for use as an index for evaluating bacterial mass under different organic loading conditions. A mathematical model was applied to evaluate the real-time PCR quantification to long-term, semi-continuous lactate fermentation. Lactate fermentation was well suited since only B. coagulans was involved in the reactions. The results obtained revealed a fundamental relationship between real-time PCR and traditional biomass analyses.
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