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      • Necessary Experimental Conditions for Single-Shot Diffraction Imaging of DNA-Based Structures with X-ray Free-Electron Lasers

        Sun, Zhibin,Fan, Jiadong,Li, Haoyuan,Liu, Huajie,Nam, Daewoong,Kim, Chan,Kim, Yoonhee,Han, Yubo,Zhang, Jianhua,Yao, Shengkun,Park, Jaehyun,Kim, Sunam,Tono, Kensuke,Yabashi, Makina,Ishikawa, Tetsuya,So American Chemical Society 2018 ACS NANO Vol.12 No.8

        <P>It has been proposed that the radiation damage to biological particles and soft condensed matter can be overcome by ultrafast and ultraintense X-ray free-electron lasers (FELs) with short pulse durations. The successful demonstration of the “diffraction-before-destruction” concept has made single-shot diffraction imaging a promising tool to achieve high resolutions under the native states of samples. However, the resolution is still limited because of the low signal-to-noise ratio, especially for biological specimens such as cells, viruses, and macromolecular particles. Here, we present a demonstration single-shot diffraction imaging experiment of DNA-based structures at SPring-8 Angstrom Compact Free Electron Laser (SACLA), Japan. Through quantitative analysis of the reconstructed images, the scattering abilities of gold and DNA were demonstrated. Suggestions for extracting valid DNA signals from noisy diffraction patterns were also explained and outlined. To sketch out the necessary experimental conditions for the 3D imaging of DNA origami or DNA macromolecular particles, we carried out numerical simulations with practical detector noise and experimental geometry using the Linac Coherent Light Source (LCLS) at the SLAC National Accelerator Laboratory, USA. The simulated results demonstrate that it is possible to capture images of DNA-based structures at high resolutions with the technique development of current and next-generation X-ray FEL facilities.</P> [FIG OMISSION]</BR>

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        Stability of Large-Size and High-Strength Steel Angle Sections Aff ected by Support Constraint

        Yun Sun,Yao-Jie Guo,Haoyuan Chen,Ke Cao 한국강구조학회 2021 International Journal of Steel Structures Vol.21 No.1

        The infl uence of support stiff ness on the eff ective calculated length which plays an essential role in the axial compression stability of steel memeber is always ignored due to the ideal support constraint that is diffi cult to achieve in the test. In this paper, the calculation method of the eff ective length factor was put forward by the mechanical model of the axially compressed member. The supports rotation stiff ness of large-size and high-strength steel angle sections (LHS) under the test condition were measured, and the eff ective length of each specimen was modifi ed. And 36 LHS members were tested under axial load. Then the stability responses for LHS members were studied using fi nite element analysis considering more section profi les and the wide range slenderness ratios. Moreover, the buckling strengths were compared with the current design codes. The results show that the support constraint has a signifi cant infl uence on the bearing capacity of the LHS member. Considering the infl uence of support restraint, when the slenderness ratio is small, the stability factor is higher than the column curve in current codes; when the slenderness ratio is large, b-curves in both GB50017-2017 and Eurocode 3 are conservative, whereas other codes are marginally unsafe. Finally, a new column curve was proposed to predict LHS members’ capacity more accurately.

      • Analysis and Design of a Bidirectional High Step-up Active Clamp Flyback Converter for Dielectric Elastomer Actuator

        Yang Chen,Yaqi Sun,Mofan Tian,Laili Wang,Haoyuan Jin 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

        Dielectric elastomer (DE) is a kind of capacitive material that widely used in soft robots’ applications as actuators. To obtain notable deformation, the drive voltage is typically up to several thousand volts and the charging charges must be removed to make it recover. In this paper, a high step-up capacitive load bi-directional flyback converter with active clamp technique is proposed. For the charging process, the effects of increasing high output voltage and parasitic elements in the circuit on the operation of the active clamp circuit are discussed in detail. Wide bandgap devices are used at the secondary side to reduce the parasitic capacitance. The proposed lossless active clamp circuit can absorb the energy in leakage inductance of transformer and achieve zero-voltage-switching (ZVS) over most of the charging process. Besides, the EMI problem caused by leakage inductance of conventional flyback converter is also solved. A prototype with 12V input and 1000V output has been fabricated and compared with a quasi-resonance flyback (QR flyback) at the same working condition. The tested results verify the good performance of the proposed ACF converter.

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