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DNA-Protein complex system using Zinc-dependent nano-self assembly
Hanjin Yu,Jihyeon Lee,Kwang Suk Lim 강원대학교 산림과학연구소 2018 강원대학교 산림과학연구소 학술대회 Vol.2018 No.09
Nonviral gene carrier uses electrostatic interaction, encapsulation, or absorption to deliver nucleic acids into the cell. Although they show high transfection efficiency, various nonviral gene delivery systems suffer from the toxicity of the cationic nature of polymeric carriers because of large amount of polymer to achieve reasonable transfection efficiencies. In previous study, we demonstrated that nano-self-assembly of nucleic acids transfected itself into cells spontaneously, without any carriers. We also showed that functional peptides were directly incorporated into M-DNA through Ligand-to-Metal Charge Transfer (LMCT). In this study, we have developed a combination system to incorporate therapeutic recombinant protein into DNA without the need for chemical conjugation. This system was based on LMCT transition, which occurs between divalent metal ions and the sulfhydryl group in cysteine-containing protein. Protein with zinc/DNA complex retarded DNA migration in agarose gel and improved transfection efficiency into the cells. This system may be useful combination systems to treat various diseases.
The protective effect of quercetin on the oxidative stress-induced substances in H9c2 cells
Hanjin Yu,Jihyeon Lee,Kwang Suk Lim 강원대학교 산림과학연구소 2018 강원대학교 산림과학연구소 학술대회 Vol.2018 No.09
Over-expression of reactive oxygen species (ROS) in the tissues lead to diabetic complications such as cardiac dysfunction, diabetic nephropathy and endothelial dysfunction. Pharmaceutical agents are required for the treatment and prevent of these complications. Quercetin is a polyphenol flavonoid, which is widely found in vegetables, grains and fruits, and is known for its antioxidant activity against oxidative stress. In this study, we determined that quercetin protected H9c2 cells against oxidative stress. To induce ROS in the cell, H9c2 cells were exposed to hydrogen peroxide (H2O2), hyperglycemia and sodium arsenite (NaAsO2). These materials were decreased the cell viability of H9c2 cells by oxidative stress induced time and concentration dependent. Quercetin strongly protected H9c2 cells under the stressful conditions by reducing intracellular ROS level and inhibiting apoptosis pathway. Quercetin may be useful therapeutic component for the treatment or prevention of myocardial infarction.
진핵생물 개시인자 유래 펩타이드의 세포 성장 억제 효능
유한진(HanJin Yu),임광석(Kwang Suk Lim) 강원대학교 산업기술연구소 2020 産業技術硏究 Vol.40 No.1
In the process of protein transcription and translation, various protein complexes bind to DNA, and all processes are precisely controlled. Among the proteins constituting this complex, a peptide derived from eukaryotic initiation factor (eIF) 2 was synthesized. In addition, in order to increase the efficiency of transduction of this peptide into cells, peptides with polyarginine, one of the protein transduction domains (PTD), were synthesized. Cell growth inhibition was confirmed in HER2 positive breast cancer (SK-Br-3) and HER2 negative breast cancer (MDA-MB-231), and cardiomyocytes (H9c2). The peptide with polyarginine had high transduction efficiency in all cells, and had excellent cancer cell growth inhibitory effects. The peptide used in this study might be useful peptide therapeutics for the treatment of cancer through future research.
유한진(HanJin Yu),홍세영(Seyoung Hong),권미지(Miji Kwon),이지현(Jihyeon Lee),박희호(Hee Ho Park),임광석(Kwang Suk Lim) 한국생물공학회 2020 KSBB Journal Vol.35 No.2
Recently, biopharmaceutical drugs take a big part in the pharmaceutical market with the development of genetic engineering. Therapeutic antibodies, one of the representative biopharmaceuticals, have strong therapeutic efficacy in various diseases such as autoimmune disease, rheumatism and cancer. Antibodies are widely used as targeted delivery systems, diagnostics and therapeutics because of their high specific recognition ability. In addition, the market for therapeutic antibodies is expanding further with the development of new biologic drugs such as biosimilar and biobetter. In particular, cancer immunotherapy has shown excellent efficacy for the treatment of cancer by regulating the immune response. In recent years, antibody-drug conjugates (ADCs), immune checkpoint inhibitors and therapeutic antibodies have been developed by many researchers and pharmaceutical companies and approved by U.S. food and drug administration as a potential strategy for the treatment of cancer. Although cancer immunotherapy has a strong therapeutic efficacy, there are still limitations to overcome such as for the treatment of solid tumor, immunogenicity and resistance. To solve these problems, new biomarkers, bispecific antibodies and combinatorial therapy with chemotherapy are being investigated. In this review, we describe the mechanism of immune response with respect to antibody therapeutics, characteristics of target cancers and discuss the potentials of cancer immunotherapy.
정한진(Hanjin Jeong),유두환(Duwhan Yu),최성만(Seongman Choi),장현수(Hyunsoo Chang) 한국추진공학회 2011 한국추진공학회 학술대회논문집 Vol.2011 No.11
추련편향 개념은 최신의 초음속 전투기에 적용되어 사용되고 있다. 본 연구는 추력편향노즐의 성능특성을 쉴리렌기법을 이용한 충격파 가시화를 통하여 제시한다. 피치 각 변화와 피치플랩길이 변화라는 추력편향노즐의 기하학적인 특성에 따른 실험평가를 수행하고, 이러한 연구를 통하여 추력편향노즐의 초음속 유동특성을 분석하였다. 추력편향노즐의 총 추력은 편향각도가 증가함에 따라 감소되며, 추력손실이 가장 적으면서 적절한 편향 효과를 가지는 최적의 플랩 길이비가 존재한다. The thrust vectoring concept has been used for use in new advanced supersonic aircraft. This study presents the performance characteristics of the thrust vectoring nozzle by visualizing the shock behaviors with Schlieren method. We performed experimental tests to see the geometrical effects of the thrust vector nozzle by changing pitch angle and length of pitch flaps. From this study we could understand the supersonic flow characteristics of the thrust vector nozzle. The total thrust of thrust vector nozzle is diminished by increasing the flap angle. But there is an optimum flap length ratio for attaining the highest thrust level and proper pitch effect.
The development of gene carrier using cellulose nanocrystal and Zinc-DNA combination systems
Jihyeon Lee,Hanjin Yu,Kwang Suk Lim 강원대학교 산림과학연구소 2018 강원대학교 산림과학연구소 학술대회 Vol.2018 No.09
To effectively delivery of nucleic acids into the cells, various gene delivery systems are required. They protect nucleic acids against immune response, serum, and elimination mechanism. Nonviral gene delivery systems have low immune response, low side effect and temporary transfection compared with viral systems. Cellulose nanocrystal (CNC) is widely used as biomedical materials because of its good mechanical properties due to their lower weight, bbiocompatibility and biodegradability. In this study, we determined that CNC-Zinc/DNA combination systems (CZD cluster) could be deliver nucleic acids into the cells. CZD cluster successfully retarded migration of nucleic acids in agarose gel and enhanced DNA transfection efficiency into the cells. CZD cluster was spontaneously formed without any chemical reaction. This cluster systems could be used to deliver any plasmid DNA or siRNA in the cells and tissues without the requirement for a specific modification of the nucleic acids.
Jihun Kim,Geonhui Lee,Hanjin Jo,Wookhyun Park,Yu Shin Jin,Ho Dong Kim,Jaehyo Kim 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.12
We suggest a wearable soft robot for post-stroke, hemiplegic patients’ finger rehabilitation and quantitative evaluations on the joint paralysis. The device consists of a pair of gloves. One measures finger positions of the normal side, and the other induces symmetric movements on the affected side by pneumatic force. Ten patients at Brunnstrom stage 3 and 4 from a local hospital participated in this study. They performed Rolyan Stacking Cones 10 times with and without the support of the soft robot. We measured subjects’ proximal interphalangeal angles and air pressures in the pneumatic glove during the exercise to monitor grab/release patterns. The soft robot helped open their paralyzed finger joints by more than 50 degrees on average regardless of Brunnstrom stages. We applied pattern recognition methods on the measurement to quantitatively evaluate the subjects. A support vector machine revealed a misclassification rate of 20%, implying that there were a considerable number of overlapping data sets near the boundary between Brunnstrom stages. K-means method with three clusters suggested a new subject group near the support vector machine border. Thus, we conclude that our wearable soft robot not only provides grab/release guides to post-stroke patients but also provides quantitative information on their finger paralysis supplementary to the existing qualitative assessments.