조리 방법에 따른 고구마줄기의 Phytochemical 함량과 항산화 활성 비교

양세희, 정혜진, 성예지, 이하나, 이세윤, 양진우, 이준수 忠北大學校 農業科學硏究所 2017 農業科學硏究 Vol.33 No.1

Vegetable consumption is associated with health benefits. Recently, the interest in these food groups has grown among due to the identification of new compound in their chemical composition. Most of the vegetables are cooked by blanching, boiling, steaming, microwaving before consumed. These cooking processes may change not only food physical characteristics but also chemical composition. The present study was undertaken to investigate the effect of different cooking methods on phytochemical content and antioxidant capacity in Ipomoea batatas (L.) Lam stem. The result of study, Total polyphenol of phytochemical in Ipomoea batatas (L.) Lam stem was 6.88-12.65 mg GAE/100 g and flavonoid was 3.16-10.46 mg CE/100 g. Compared with the raw of Ipomoea batatas (L.) Lam stem, all cooking methods were found to significant correlations between the antioxidant activity assessed by ABTS, DPPH and polyphenol(p<0.05). These results can be provide the basic data how to prevent lose of nutrition in Ipomoea batatas (L.) Lam stem when cooking.

Cdon deficiency causes cardiac remodeling through hyperactivation of WNT/β-catenin signaling

Jeong, Myong-Ho,Kim, Hyun-Ji,Pyun, Jung-Hoon,Choi, Kyu-Sil,Lee, Dong I.,Solhjoo, Soroosh,O'Rourke, Brian,Tomaselli, Gordon F.,Jeong, Dong Seop,Cho, Hana,Kang, Jong-Sun National Academy of Sciences 2017 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.114 No.8

<P>On pathological stress, Wnt signaling is reactivated and induces genes associated with cardiac remodeling and fibrosis. We have previously shown that a cell surface receptor Cdon (cell-adhesion associated, oncogene regulated) suppresses Wnt signaling to promote neuronal differentiation however its role in heart is unknown. Here, we demonstrate a critical role of Cdon in cardiac function and remodeling. Cdon is expressed and predominantly localized at intercalated disk in both mouse and human hearts. Cdon-deficient mice develop cardiac dysfunction including reduced ejection fraction and ECG abnormalities. Cdon(-/-) hearts exhibit increased fibrosis and up-regulation of genes associated with cardiac remodeling and fibrosis. Electrical remodeling was demonstrated by up-regulation and mislocalization of the gap junction protein, Connexin 43 (Cx43) in Cdon(-/-) hearts. In agreement with altered Cx43 expression, functional analysis both using Cdon(-/-) cardiomyocytes and shRNA-mediated knockdown in rat cardiomyocytes shows aberrant gap junction activities. Analysis of the underlying mechanism reveals that Cdon(-/-) hearts exhibit hyperactive Wnt signaling as evident by beta-catenin accumulation and Axin2 up-regulation. On the other hand, the treatment of rat cardiomyocytes with a Wnt activator TWS119 reduces Cdon levels and aberrant Cx43 activities, similarly to Cdon-deficient cardiomyocytes, suggesting a negative feedback between Cdon and Wnt signaling. Finally, inhibition of Wnt/beta-catenin signaling by XAV939, IWP2 or dickkopf (DKK)1 prevented Cdon depletion-induced up-regulation of collagen 1a and Cx43. Taken together, these results demonstrate that Cdon deficiency causes hyperactive Wnt signaling leading to aberrant intercellular coupling and cardiac fibrosis. Cdon exhibits great potential as a target for the treatment of cardiac fibrosis and cardiomyopathy.</P>

TAZ as a novel enhancer of MyoD‐mediated myogenic differentiation

Jeong, Hana,Bae, Sujung,An, Su Yeon,Byun, Mi Ran,Hwang, Jun‐,Ha,Yaffe, Michael B.,Hong, Jeong‐,Ho,Hwang, Eun Sook Federation of American Society for Experimental Bi 2010 The FASEB Journal Vol.24 No.9

<P>Myoblast differentiation is indispensable for skeletal muscle formation and is governed by the precisely coordinated regulation of a series of transcription factors, including MyoD and myogenin, and transcriptional coregulators. TAZ (transcriptional coactivator with PDZ-binding motif) has been characterized as a modulator of mesenchymal stem cell differentiation into osteoblasts and adipocytes through its regulation of lineage-specific master transcription factors. In this study, we investigated whether TAZ affects myoblast differentiation, which is one of the differentiated lineages of mesenchymal stem cells. Ectopic overexpression of TAZ in myoblasts increases myogenic gene expression in a MyoD-dependent manner and hastens myofiber formation, whereas TAZ knockdown delays myogenic differentiation. In addition, enforced coexpression of TAZ and MyoD in fibroblasts accelerates MyoD-induced myogenic differentiation. TAZ physically interacts with MyoD through the WW domain and activates MyoD-dependent gene transcription. TAZ additionally enhances the interaction of MyoD with the myogenin gene promoter. These results strongly suggest that TAZ functions as a novel transcriptional modulator of myogenic differentiation by promoting MyoD-mediated myogenic gene expression.</P>

Potential role of suppressors of cytokine signaling in M1 vs M2 macrophage function during colitis-associated cancer development in mice

Hana Jeong,Choong-Eun Lee 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.1

SOCS3 Attenuates Dexamethasone-Induced M2 Polarization by Down-Regulation of GILZ via ROS- and p38 MAPK-Dependent Pathways

Jeong Hana,Yoon Hyeyoung,Lee Yerin,Kim Jun Tae,Yang Moses,Kim Gayoung,Jung Bom,Park Seok Hee,이충은 대한면역학회 2022 Immune Network Vol.22 No.4

Suppressors of cytokine signaling (SOCS) have emerged as potential regulators of macrophage function. We have investigated mechanisms of SOCS3 action on type 2 macrophage (M2) differentiation induced by glucocorticoid using human monocytic cell lines and mouse bone marrow-derived macrophages. Treatment of THP1 monocytic cells with dexamethasone (Dex) induced ROS generation and M2 polarization promoting IL-10 and TGF-β production, while suppressing IL-1β, TNF-α and IL-6 production. SOCS3 over-expression reduced, whereas SOCS3 ablation enhanced IL-10 and TGF-β induction with concomitant regulation of ROS. As a mediator of M2 differentiation, glucocorticoid-induced leucine zipper (GILZ) was down-regulated by SOCS3 and up-regulated by shSOCS3. The induction of GILZ and IL-10 by Dex was dependent on ROS and p38 MAPK activity. Importantly, GILZ ablation led to the inhibition of ROS generation and anti-inflammatory cytokine induction by Dex. Moreover, GILZ knock-down negated the up-regulation of IL-10 production induced by shSOCS3 transduction. Our data suggest that SOCS3 targets ROS- and p38-dependent GILZ expression to suppress Dex-induced M2 polarization.

Transcriptional coactivator with PDZ ‐binding motif is required to sustain testicular function on aging

Jeong, Mi Gyeong,Song, Hyuna,Shin, Ji Hyun,Jeong, Hana,Kim, Hyo Kyeong,Hwang, Eun Sook BLACKWELL PUBLISHING 2017 AGING CELL Vol.16 No.5

<P><B>Summary</B></P><P>Transcriptional coactivator with PDZ‐binding motif (TAZ) directly interacts with transcription factors and regulates their transcriptional activity. Extensive functional studies have shown that TAZ plays critical regulatory roles in stem cell proliferation, differentiation, and survival and also modulates the development of organs such as the lung, kidney, heart, and bone. Despite the importance of TAZ in stem cell maintenance, TAZ function has not yet been evaluated in spermatogenic stem cells of the male reproductive system. Here, we investigated the expression and functions of TAZ in mouse testis. TAZ was expressed in spermatogenic stem cells; however, its deficiency caused significant structural abnormalities, including atrophied tubules, widened interstitial space, and abnormal Leydig cell expansion, thereby resulting in lowered sperm counts and impaired fertility. Furthermore, TAZ deficiency increased the level of apoptosis and senescence in spermatogenic cells and Leydig cells upon aging. The expression of senescence‐associated β‐galactosidase (SA‐βgal), secretory phenotypes, and cyclin‐dependent kinase inhibitors (p16, p19, and p21) significantly increased in the absence of TAZ. TAZ downregulation in testicular cells further increased SA‐βgal and p21 expression induced by oxidative stress, whereas TAZ overexpression decreased p21 induction and prevented senescence. Mechanistic studies showed that TAZ suppressed DNA‐binding activity of p53 through a direct interaction and thus attenuated p53‐induced p21 gene transcription. Our results suggested that TAZ may suppress apoptosis and premature senescence in spermatogenic cells by inhibiting the p53‐p21 signaling pathway, thus playing important roles in the maintenance and control of reproductive function.</P>

Database metadata standardization processing model using web dictionary crawling

Hana Jeong,Koo-Rack Park,Young-suk Chung 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.9

Hesperedin promotes MyoD-induced myogenic differentiationin vitroandin vivo : Enhanced myogenic differentiation by hesperedin

Jeong, Hana,Lee, Joo Yeon,Jang, Eun Jung,Lee, Eun Hye,Bae, Myung Ae,Hong, Jeong-Ho,Hwang, Eun Sook Wiley (Blackwell Publishing) 2011 British journal of pharmacology Vol.163 No.3

FEROM: Feature Extraction and Refinement for Opinion Mining

Hana Jeong,Dongwook Shin,최중민 한국전자통신연구원 2011 ETRI Journal Vol.33 No.5

Opinion mining involves the analysis of customer opinions using product reviews and provides meaningful information including the polarity of the opinions. In opinion mining, feature extraction is important since the customers do not normally express their product opinions holistically but separately according to its individual features. However, previous research on feature-based opinion mining has not had good results due to drawbacks, such as selecting a feature considering only syntactical grammar information or treating features with similar meanings as different. To solve these problems, this paper proposes an enhanced feature extraction and refinement method called FEROM that effectively extracts correct features from review data by exploiting both grammatical properties and semantic characteristics of feature words and refines the features by recognizing and merging similar ones. A series of experiments performed on actual online review data demonstrated that FEROM is highly effective at extracting and refining features for analyzing customer review data and eventually contributes to accurate and functional opinion mining.

IPA를 활용한 서울시민리그(S-리그) 운영 속성에 관한 연구

정하나(Jeong, Hana),김태환(Kim, Taehwan),황선환(Hwnag, Sunhwan) 한국여가문화학회 2021 여가학연구 Vol.19 No.4

이 연구는 2019년 서울특별시가 주최·주관한 서울시민리그(S-리그) 참여자의 리그 운영 속성에 관한 중요도, 만족도 조사연구 자료를 바탕으로 IPA를 통해 서울시민리그 참여를 유도하고 지속시킬 수 있는 속성들을 도출하는데 목적이 있다. 성별, 종목별, 연령별로 비례할당표집된 참여자의 조사자료 675부를 최종분석한 결과는 다음과 같다. 첫째, S-리그 운영 속성에 관한 중요도와 만족도의 차이 검증 결과 모든 항목에서 유의한 차이가 검증되었다. 모든 항목들의 중요도가 만족도 보다 높게 나타나, 참여자의 기대수준에 미치지 못한 것으로 나타났다. 둘째, S-리그 운영 속성에 관한 중요도-만족도의 IPA 분석결과 현상유지영역에서는 참가비, 승점제 운영방식, 우승팀 보상의 적절성, 관람석 이용 편의성 및 충분성 항목이 도출되었고, 지속유지영역에서는 참가자 모집시기, 심판 운영 체계, 시설 및 인프라 항목이 도출되었다. 노력유지영역에서는 홍보 및 관심유도 활동, 상대 팀 불참 시 대응 방식이 도출되었고, 중점개선영역에서는 질의/제안에 대한 피드백, 주무기관의 통합적 대응, 결과 공지 시점 및 신속성, 리그 기간, 불참 팀에 대한 패널티 제도, 연습시설 이용 편의성 항목이 도출되었다. This study derives the attributes that can induce participation in the Seoul Citizens League based on the importance and satisfaction survey data on the league operation attributes of the Seoul Citizens League (S-League) participants hosted and supervised by the Seoul Metropolitan Government in 2019. There is a purpose. The results of the final analysis of 675 survey data of participants who were proportionally sampled by gender, event, and age are as follows. First, as a result of verifying the difference in importance and satisfaction regarding S-League operational attributes, significant differences were verified in all items. The importance of all items was higher than the satisfaction level, indicating that it did not meet the expectations of the participants. Second, as a result of the IPA analysis of importance-satisfaction regarding the S-League operation attributes, the participation fee, the points system operation method, the appropriateness of the winning team reward, convenience and sufficiency of the use of the spectators were derived in the status quo area, and the participant recruitment period in the maintenance area. , referee operating system, facilities and infrastructure items were derived. In the effort maintenance area, public relations and interest-inducing activities and response methods were derived when the opposing team does not participate, and in the focus improvement area, feedback on questions/suggestions, integrated response of the competent authority, timing and promptness of result announcement, league period, and non-participation team were derived. Penalty system and convenience of use of practice facilities were derived.