Experience of surgical treatment in a granular cell tumor in the qscending colon: a case report

Kim In-Kyeong,Ju Young-Tae,Kim Han-Gil,Lee Jin-Kwon,Kim Dong-Chul,Kim Jae-Myung,Cho Jin Kyu,Park Ji-Ho,Kim Ju-Yeon,Jeong Chi-Young,Hong Soon-Chan,Kwag Seung-Jin 대한대장항문학회 2023 Annals of Coloproctolgy Vol.39 No.3

We report a case about successful surgical treatment of a granular cell tumor in the ascending colon. A 36-year-old man underwent screening colonoscopy. An endoscopic examination revealed a 10-mm yellowish and hemispheric mass in the ascending colon, and lower endoscopic ultrasonography revealed a hypoechoic-to-isoechoic mass invaded the submucosal layer. The mass was suspected to be a colonic carcinoid tumor. Based on the preoperative evaluation, endoscopic complete resection was considered difficult. Therefore, the lesion was removed via laparoscopic right hemicolectomy. Histological examination revealed that the tumor consisted of nests of polygonal cells with abundant granular eosinophilic cytoplasm. Immunohistochemical staining revealed diffuse positivity for S100 and CD68. Therefore, the tumor was diagnosed as a granular cell tumor. We suggest that surgical resection should be considered if it is located in the thin-walled ascending colon prone to perforation, difficult to rule out malignant tumor due to submucosal invasion, or to remove endoscopically.

Estimation of Acute Infarct Volume with Reference Maps: A Simple Visual Tool for Decision Making in Thrombectomy Cases

Kim, Dong-Eog,Ryu, Wi-Sun,Schellingerhout, Dawid,Jeong, Han&#x2010,Gil,Kim, Paul,Jeong, Sang-Wuk,Park, Man-Seok,Choi, Kang-Ho,Kim, Joon-Tae,Kim, Beom Joon,Han, Moon-Ku,Lee, Jun,Cha, Jae-Kwan,Kim, Dae- Korean Stroke Society 2019 Journal of stroke Vol.21 No.1

<P><B>Background and Purpose</B></P><P>Thrombectomy within 24 hours can improve outcomes in selected patients with a clinical-infarct mismatch. We devised an easy-to-use visual estimation tool that allows infarct volume estimation in centers with limited resources. </P><P><B>Methods</B></P><P>We identified 1,031 patients with cardioembolic or large-artery atherosclerosis infarction on diffusion-weighted images (DWIs) obtained before recanalization therapy and within 24 hours of onset, and occlusion of the internal carotid or middle cerebral artery. Acute DWIs were mapped onto a standard template and used to create visual reference maps with known lesion volumes, which were then used in a validation study (with 130 cases) against software estimates of infarct volume. </P><P><B>Results</B></P><P>The DWI reference map chart comprises 144 maps corresponding to 12 different infarct volumes (0.5, 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, and 19 mL) in each of 12 template slices (Montreal Neurological Institute z-axis –15 to 51 mm). Infarct volume in a patient is estimated by selecting a slice with a similar infarct size at the corresponding z-axis level on the reference maps and then adding up over all slices. The method yielded good correlations to software volumetrics and was easily learned by both experienced and junior physicians, with approximately 1 to 2 minutes spent per case. The sensitivity, specificity, and accuracy for detecting threshold infarct volumes (<21, <31, and <51 mL) were very high (all about >90%). </P><P><B>Conclusions</B></P><P>We developed easy-to-use reference maps that allow prompt and reliable visual estimation of infarct volumes for triaging patients to thrombectomy in acute stroke.</P>

Activation of Rac1-dependent redox signaling is critically involved in staurosporine-induced neurite outgrowth in PC12 cells

Kim, Du Sik,An, Jeong Mi,Lee, Han Gil,Seo, Su Ryeon,Kim, Seon Sook,Kim, Ju Yeon,Kang, Jeong Wan,Bae, Yun Soo,Seo, Jeong Taeg Informa Healthcare 2013 Free radical research Vol.47 No.2

<P>Staurosporine, a non-specific protein kinase inhibitor, has been shown to induce neurite outgrowth in PC12 cells, but the mechanism by which staurosporine induces neurite outgrowth is still obscure. In the present study, we investigated whether the activation of Rac1 was responsible for the neurite outgrowth triggered by staurosporine. Staurosporine caused rapid neurite outgrowth independent of the ERK signaling pathways. In contrast, neurite outgrowth in response to staurosporine was accompanied by activation of Rac1, and the Rac1 inhibitor NSC23766 attenuated the staurosporine-induced neurite outgrowth in a concentration-dependent manner. In addition, suppression of Rac1 activity by expression of the dominant negative mutant Rac1N17 also blocked the staurosporine-induced morphological differentiation of PC12 cells. Staurosporine caused an activation of NADPH oxidase and increased the production of reactive oxygen species (ROS), which was prevented by NSC23766 and diphenyleneiodonium (DPI), an NADPH oxidase inhibitor. Staurosporine-induced neurite outgrowth was attenuated by pretreatment with DPI and exogenous addition of sublethal concentration of H<SUB>2</SUB>O<SUB>2</SUB> accelerated neurite outgrowth triggered by staurosporine. These results indicate that activation of Rac1, which leads to ROS generation, is required for neurite outgrowth induced by staurosporine in PC12 cells.</P>

Phenolic Glycosides from the Twigs of <i>Salix glandulosa</i>

Kim, Chung Sub,Kwon, Oh Wook,Kim, Sun Yeou,Choi, Sang Un,Kim, Jae Yoon,Han, Ji Young,Choi, Soo Im,Choi, Jong Gil,Kim, Ki Hyun,Lee, Kang Ro American Chemical Society and American Society of 2014 Journal of natural products Vol.77 No.8

<P>As a part of an ongoing search for bioactive constituents from Korean medicinal plants, the phytochemical investigations of the twigs of <I>Salix glandulosa</I> afforded 12 new phenolic glycosides (<B>1</B>–<B>12</B>) and a known analogue (<B>13</B>). The structures of <B>1</B>–<B>13</B> were characterized by a combination of NMR methods (<SUP>1</SUP>H and <SUP>13</SUP>C NMR, <SUP>1</SUP>H–<SUP>1</SUP>H COSY, HMQC, and HMBC), chemical hydrolysis, and GC/MS. The absolute configuration of <B>13</B> [(1<I>R</I>,2<I>S</I>)-2-hydroxycyclohexyl-2′-<I>O</I>-<I>trans</I>-<I>p</I>-coumaroyl-β-<SMALL>d</SMALL>-glucopyranoside] was determined for the first time. Compounds <B>1</B>–<B>3</B>, <B>6</B>, and <B>7</B> exhibited inhibitory effects on nitric oxide production in lipopolysaccharide-activated murine microglial cells (IC<SUB>50</SUB> values in the range 6.6–20.5 μM).</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2014/jnprdf.2014.77.issue-8/np500488v/production/images/medium/np-2014-00488v_0003.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np500488v'>ACS Electronic Supporting Info</A></P>

Effect of Long-Term Treatment with Fimasartan on Transient Focal Ischemia in Rat Brain

Kim, Chi Kyung,Yang, Xiu-Li,Kim, Young-Ju,Choi, In-Young,Jeong, Han-Gil,Park, Hong-Kyun,Kim, Dohoung,Kim, Tae Jung,Jang, Hyunduk,Ko, Sang-Bae,Yoon, Byung-Woo Hindawi Publishing Corporation 2015 BioMed research international Vol.2015 No.-

<P>Fimasartan is a newly developed angiotensin receptor blocker, which may have protective effects during myocardial infarction or atherosclerosis. In this context, we investigated the effects of long-term treatment with low-dose fimasartan on focal ischemia in rat brain. We induced focal ischemia in brain by transient intraluminal occlusion of middle cerebral artery (MCA) and administered low-dose (0.5 mg/kg) or regular doses (1 or 3 mg/kg) of fimasartan via intravenous routes. After the administration of low-dose (0.5 mg/kg) fimasartan, blood pressure did not decrease compared to the phosphate-buffered saline- (PBS-) control with MCA occlusion (MCAO) group. The infarct volume and ischemic cell death were reduced in the low-dose fimasartan-treated group (46 ± 41 mm<SUP>3</SUP> for 0.5 mg/kg and 153 ± 47 mm<SUP>3</SUP> for PBS-control with MCAO; <I>P</I> < 0.01) but not in the regular-dose groups. Low-dose fimasartan treatment improved functional recovery after ischemia and significantly decreased mortality. In our study, fimasartan reduced the degradation of I<I>κ</I>B and the formation of an inflammatory end-product, COX-2. As a result, the recruitment of inflammatory cells in the peri-infarct area decreased in fimasartan-treated group. We have demonstrated that long-term, low-dose fimasartan treatment improved outcomes after focal ischemia in the brain via a reduction of inflammation.</P>

Tunable Bandgap Narrowing Induced by Controlled Molecular Thickness in 2D Mica Nanosheets

Kim, Sang Sub,Khai, Tran Van,Kulish, Vadym,Kim, Yoon-Hyun,Na, Han Gil,Katoch, Akash,Osada, Minoru,Wu, Ping,Kim, Hyoun Woo American Chemical Society 2015 Chemistry of materials Vol.27 No.12

<P>Bandgap engineering of atomically thin 2D crystals is critical for their applications in nanoelectronics, optoelectronics, and photonics. Here, we report a simple but rather unexpected approach for bandgap engineering of muscovite-type mica nanosheets (KAl<SUB>3</SUB>Si<SUB>3</SUB>O<SUB>10</SUB>(OH)<SUB>2</SUB>) via controlled molecular thickness. Through density functional calculations, we analyze electronic structures in 2D mica nanosheets and develop a general picture for tunable bandgap narrowing induced by controlled molecular thickness. From conducting atomic force microscopy, we observe an abnormal bandgap narrowing in 2D mica nanosheets, contrary to well-known quantum size effects. In mica nanosheets, decreasing the number of layers results in reduced bandgap energy from 7 to 2.5 eV, and the bilayer case exhibits a semiconducting nature with ∼2.5 eV. Structural modeling by transmission electron microscopy and density functional calculations reveal that this bandgap narrowing can be defined as a consequence of lattice relaxations as well as surface doping effects. These bandgap engineered 2D mica nanosheets open up an exciting opportunity for new physical properties in 2D materials and may find diverse applications in 2D electronic/optoelectronic devices.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cmatex/2015/cmatex.2015.27.issue-12/cm504802j/production/images/medium/cm-2014-04802j_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cm504802j'>ACS Electronic Supporting Info</A></P>

Proteome analysis of the anti-inflammatory response of flavonoids isolated from Korean Citrus aurantium L. in lipopolysaccharide-induced L6 rat skeletal muscle cells.

Kim, Jin-A,Park, Hyeon-Soo,Park, Kwang-Il,Hong, Gyeong-Eun,Nagappan, Arulkumar,Zhang, Jue,Han, Dae-Yong,Shin, Sung-Chul,Won, Chung-Gil,Kim, Eun-Hee,Kim, Gon-Sup Institute for Advanced Research in Asian Science a 2013 The American journal of Chinese medicine Vol.41 No.4

<P>Skeletal muscle is an important organ in our body and a dynamic composite of proteins. Citrus aurantium L. has been widely used in oriental medicine in Eastern Asia for a long time. It contains over 100 bioactive compounds and flavonoids that regulate the inflammatory response and tumorigenesis, through various mechanisms. In the present study, we investigated changes in the protein pattern using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization time of flight mass spectroscopy (MALDI-TOF/MS) to assess the anti-inflammatory effect of flavonoids isolated from Korean C. aurantium L. in lipopolysaccharide (LPS)-induced L6 cells. L6 skeletal muscle cells were pretreated with flavonoids for 1 h and stimulated with LPS for 24 h. Proteins from the L6 cells of the control, LPS treated and flavonoid treated groups were extracted and resolved by 2-DE using pH 4-7 IPG strips loaded with 150 μg of protein. Forty-one differentially expressed protein spots were identified (more than two-fold was considered significant, p < 0.05), and 18 were detected by MALDI-TOF/MS. These results suggest that proteomics can be used to identify changes in the expression of marker proteins and the anti-inflammatory effect of flavonoids isolated from Korean C. aurantium L.</P>

Mating behavior of Pine Sawyer, Monochamus saltuarius Gebler (Coleoptera: Cerambycidae)

Kim, Min-Ki,Kim, Ju-Sub,Han, Ju-Hwan,Kim, Young-Jae,Yoon, Chang-Mann,Kim, Gil-Hah Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.3

Mating behavior of the pine sawyer, Monochamus saltuarius Gebler (Cerambycidae: Lamiinae) was observed on pine trees in an outdoor net cage kept in one healthy pine tree and a cluster of pinelogs (cut over one month) to attract the beetles. When sawyer adult pairs released into net cage, they all moved to the pinelogs. And then the male was staying motionless with his antenna outstretched while the female was actively moving in his vicinity. The mating behavioral reactions were followed by the next steps: The first phase of courtship was initiated by the female approach toward the male. Second, male dashed, contacted antenna and mounted female's back. Third, female carried the male on her back and walked around and then male began to lick. Fourth, male inserted his penis into the female's genitalia when female stopped walking. While a long pair-bond, the mating pair repeated copulations. lastly, when mating is over, male and female stayed separately. The mating began on sunset and resting of both male and female began at the temperature below about $20^{\circ}C$. Substantially straightforward mate searching by female indicates the presence of a male sex pheromone.

Analysis of the Cellular Stress Response in MCF10A Cells Exposed to Combined Radio Frequency Radiation

KIM, Han-Na,HAN, Na-Kyung,HONG, Mi-Na,CHI, Sung-Gil,LEE, Yun-Sil,KIM, Taehong,PACK, Jeong-Ki,CHOI, Hyung-Do,KIM, Nam,LEE, Jae-Seon Journal of Radiation Research Editorial Committee 2012 JOURNAL OF RADIATION RESEARCH Vol.53 No.2

<P>Exposure to environmental stressors can be measured by monitoring the cellular stress response in target cells. Here, we used the cellular stress response to investigate whether single or combined radio frequency (RF) radiation could induce stress response in human cells. Cellular stress responses in MCF10A human breast epithelial cells were characterized after exposure to 4 h of RF radiation [code division multiple access (CDMA) or CDMA plus wideband CDMA (WCDMA)] or 2 h RF radiation on 3 consecutive days. Specific absorption rate (SAR) was 4.0 W/kg for CDMA signal alone exposure and 2.0 W/kg each, 4.0 W/kg in total for combined CDMA plus WCDMA signals. Expression levels and phosphorylation states of specific heat shock proteins (HSPs) and mitogen-activated protein kinases (MAPKs) were analyzed by Western blot. It was found that HSP27 and ERK1/2 phosphorylations are the most sensitive markers of the stress response in MCF10A cells exposed to heat shock or ionizing radiation. Using these markers, we demonstrated that neither one-time nor repeated single (CDMA alone) or combined (CDMA plus WCDMA) RF radiation exposure significantly altered HSP27 and ERK1/2 phosphorylations in MCF10A cells (<I>p</I> > 0.05). The lack of a statistically significant alteration in HSP27 and ERK1/2 phosphorylations suggests that single or combined RF radiation exposure did not elicit activation of HSP27 and ERK1/2 in MCF10A cells.</P>

Possible Presence of Pheromone in Mating Behavior of the Pine Sawyer Monochamus saltuarius Gebler (Coleoptera: Cerambycidae)

Kim, Ju-Sub,Kim, Min-Ki,Han, Ju-Hwan,Yoon, Chang-Mann,Choi, Kwang-Sik,Shin, Sang-Chul,Kim, Gil-Hah Korean Society of Applied Entomology 2006 Journal of Asia-Pacific Entomology Vol.9 No.4

The mating behavior of the pine sawyer, Monochamus saltuarius, is composed of two phases. The first phase is the female approach to pheromone-releasing males. When bioassayed by T-tube olfactometer, the female was moved toward the male and hexane extract of the male. The second phase is the male mounted the female to copulate after perceiving the contact pheromone being on the female body surface. When the hexane extract of the female body surface was applied onto a glass rod (dummy), the male showed the mating behavior to the dummy. The component of female body surface extract fractionated using a silicic acid column elicited mating behaviors of the males, and the other component by ether fraction was showed a high copulatory attempt. This study was discussed two-step functions of the pheromones in the beetle mating behavior.