Costs and Productivities of Forest Biomass Harvesting Operations in the US

( Han Sup Han1 ),( Sang Kyun Han ),( Du Song Cha ),( Nam Hun Kim ) 한국목재공학회 2013 한국목재공학회 학술발표논문집 Vol.2013 No.-

세계적 패러다임 변화에 따른 도심형 한옥의 발전방향에 관한 조사 연구

한종훈(Han, Jong Hun),박미진(Park, Mi, Jin),곽동화(Kwack Dong Wha) 대한건축학회 2012 대한건축학회 학술발표대회 논문집 Vol.32 No.2

뇌종양의 악성도 판정을 위한 분열기 세포측정에 관한 연구

한용표,변진수,허철,홍순기,김헌주,조미연,정순희,김호근 대한신경외과학회 1992 Journal of Korean neurosurgical society Vol.21 No.11

Tissue markers of cellular proliferation have been utilized as prognostic indicators in tumors of the central nervous system. Nucleolar organizer regions represent transcriptionally active sites of ribosomal deoxyribonucleic acid and can be identified by a simple argyrophilic technique. The authors describe a standardized approach to the assessment of these argyrophilic nucleolar organizer regions(AgNORs) in 25 astrocytomas(including 6 glioblastomas), 6 oligodendrogliomas and 1 xanthochromic astrocytoma, which were also estimated in association with 2-year survival post-operatively. Appropriate sections were silver stained and AgNORs were counted in 1000 cells. In cases of astrocytoma, the mean AgNORs/cell count of group survived more than 2 years after operation was significantly different from that survived less than 2 years : 1.93±1.13 vs. ≥ 4.79±3.90. And the mean AgNORs/cell count was 1.53±0.32 in low grade astrocytoma. 2.03±0.59 in grade Ⅲ astrocytoma, and ≥6.64±3.77 in grade Ⅳ astrocytoma. Among the patients with the same grade astrocytoma, the mean AgNORs/cell count was lower in the cases of long survival rather than short survival. The assessment of AgNORs can be easily performed by any surgical pathology laboratory without specialized equipment. It may be used as a valuable and independent prognosticator of biological behavior in cases of astrocytoma.

혈관평활근세포에서 산화에너지대사 억제에 의한 아밀로이드전구단백질 대사의 변화

한문구,최웅,김헌식,안희열,한설희 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.1

Background: A reduction in the activity of cytochrome c oxidase(COX) has been recently identified in mitochondria from platelets and postmortem brain tissue of AD patients Sodium azide (NaN₃). a COX inhibitor, is an effective chemical agent producing energy shortage and oxidative stress both in vitro and in vivo system Furthermore it has been suggested that vascular compromise could be either directly involved AD pathogenesis or indirectly associated with triggering pathogenetic events leading to AD This study was performed to investigate amyloid precursor protein (APP) metabolism by inhibition of mitochondrial energy metabolism in cultured vascular smooth muslce cells (VSMCs) Materials and Methods: VSMCs isolated from the aorta of seven weeks old Spraque-Dawley rat were treated with NaN₃in a low concentration (100-500μM) or in a high concentration (1-100mM) Cellular proliferation and viability were determined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenly)-2H-tetrazolium) assay Cellular APP was detected with N-terminal specific antibody 22C11. Celldeath was determined by observation of morphology and terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick end labeling stain (TUNEL) We used ginkgo biloba extract(EGb761) and melatonin as anti-oxidants to investigate the mechanism of latered APP metabolism Results: The viability of VSMCs was increased after treatment with 1 mM and 10mM NaN₃(p<0.05) unitl 3 hr and then dimnished Many TUNEL positive cells were found in 10mM and 100mM treatment group. but were not apoptotic in nature 22C11 immunoreactivity was not changed at 3 hr, 6hr, 12hr Anti-oxidants reduced cellular proliferation (p<0.05). but did not block TUNEL positivities and did not influence the 22C11 immunoreactivity In a low concentration NaN₃ treatment group the viability of VSMCs was increased concentration dependently(p<0.05) Immunoblot with 22C11 showed the concentration dependent decrease at 145 kDa, 125 kDa. and high molecular weight range (>160kDa) TUNEL staining showed DNA fragmentations and condensations of nuclear chromatin suggesting apoptosis After treatment with anti-oxidants, the cellular proliferation was more decreased (p<0.05), and TUNEL positive cell deaths were blocked Immunoreactivities of 125 kDa (immature APP). 145 kDa (mature APP). and higher molecular weight bands were recovered below 400μM of NaN₃ Immunoreactivity of 145 kDa was recovered in 100 μM NaN₃ treated group Conclusions: The presumed mechanism of low concentration COX inhibitor is the overproduction of reactive oxygen species resulting from a depression of the mitochondiral electron transport chain. whereas potential consequence of high concentration COX inhibitor might be related to decreassion of ATP synthesis and bioenergetic impairment Reactive oxygen radicals in response to low concentration COX inhibitor alter the processing of APP in VSMCs This investigation demonstrated analtered APP metabolism as a peripheral marker of AD Therefore VSMCs treated with low concentration COX inhibitor could be concsidered as a novel in vitro model of AD.

흰쥐 중격핵의 아미노산 함량에 대한 해마 제거의 영향

한영길,김종규,양훈모 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

A study was planned to analyze amino acid in target structure -the septal mucleus-of hippocampectomized rats by high-performance liquid chromatograph (HPLC). 33 female rats were divided into 3 groups, of which 8 rats had their hippocampal tissue on both sides removed through an opening in the parieto-occipital cortex (hippocampal group), 7 rats received damage to the parieto-occipital cortex only (cortical control group), and 8 rats serveed as normal control animals. One week later, the animals were sacrificed by decapitationin the cold room. Two to three mg of tissue was obtained from the septal nucleus in one side of the brain. The tissue three mg of tissue was obtained from the septal nucleus in one side of the brain. The tissue samples were homogenized in 200 of 0.5M perchloric acid in 1mM EDTA with ground-glass homogenizers. After centrifugation at 3.500 rpm for 15 min, an aliquot of 80 supernatant was neutralized with 40㎕ of 2.0M KHCO and then centrifuged again at 3,500 rpm for 10min. An aliquot of 20㎕ was taken and diluted with 350㎕ β-mercaptopropionic acid in 10 ml of 100 mM sodium borate buffer (Ph 9.1) and 350㎕ of OPA solved in ethanol which was diluted with 10ml of 100 mM sodium borate buffer (Ph 9.1) Then 400㎕ of the resulting sample was injected auto injector. Peak areas were automatically integrated and calculated by chromatopac. In the septal nucleus, aspartate increased a little in the hippocampla group and the cortical control group than in the normal control group . while there were no significant difference between the 3 groups. Glutamate decreased significantly in the hippocampla group than in the normal control group (P<0.001) and the cortical control group (P<0.005), while there were no significant difference between the 2 control groups. In the serine, glycine, threonine and alanine, the differences among 3 group values were nonsignificant. It is inferred from the above mentioned results that excitatory transmitter substances employ the glutamate in the septal nucleus, and that the hippocampus was facilitatory to the septal nucleus.

뇌졸중과 동반된 병원감염

한범기,이지용,이성수,김헌주 연세대학교 원주의과대학 2000 원주의대논문집 Vol.13 No.1

정신증상을 수반한 급성 간헐성 폴피리아의 1예 보고

한상엽,오민웅,김헌석,최수호 大韓神經精神醫學會 1972 신경정신의학 Vol.11 No.2

This is a brief report on a case Acute Intermittent Porphyria observed in the St. Mary's Hospital, Catholic Medical College, Seoul, Korea. The case was originally admitted to the internal medicine department where initial diagnostic workup was made. The case, however, showed various psychoneurotic signs which impressed both internists as well a psychiatrists who saw the case upon consultation that the patient might as well be suffered from hysteria. Consequently the Patient was transfered to the psychiatric free-ward where further observation, family study as well as various laboratory tests thought to necessary, were done. While family study revealed(see pedigree) that one of the patient's aunt and her mother had suffered from the illness which seemed similar with the patient's present illness, and two of her siblings were also found to have suffered from the almost identical illness in the past. Examination of urine also revealed excess in amount of porphobilinogen and uroporphyrin in the urine. Authors stressed the importance 1) interdepartmental consultation, and also of 2) the needs of careful review and evaluation of the results of the basic and further laboratory tests for which paychiatrists tend to pay less attention to those cases with neurotic manifestations.

하악골 후방이동시 중안면부 연조직의 변화양상에 대한 임상통계학적 연구

한대희,김수남,민승기,김태성,성헌모 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.3

Prediction of the soft tissue changes following hard tissue movement is very important from the esthetic view point for patients who have orthognathic surgery. There are many cephalometric analysis of facial bone and soft tissue on the lower lip and chin region but few soft tissue analysis on the midface after mandibular setback surgery. This study was performed to obtain whether the mandibular posterior movement has influence on the midface and the predictable ratio of post-operative measurement values of the soft tissue changes following mandibular setback surgery. Fifteen patients (8 males and 7 females) who had undergone mandibular setback surgery were selected and analyzed the soft tissue movement on the upper lip and the cheek region. Post-operative changes of the soft tissue measurements after mandibular surgery were examined on pre- and post-operative cephalo-metrics and the ratio of changes were analyzed after drawing the reference line on the face with the barium sulfate solution. The reference lines were perpendicular to the intercanthal line from infraorbital foramen and lateral canthus. The results obtained were as follows : 1.There were tendancy of anterior movement of soft tissue adjacent the nose after mandibular surgery 2.There were incerased tendancy of the amount of anterior movement from the nasal crease to the cheek region. 3.The amount of anterior movement of the soft tissue was larger below the palatal plane compared with above the palatal plane in the cheek region. 4.The upper lip length was increased and moved posterior direction after mandibular setback surgery 5.The lower lip was moved posterior direction by posterior movement of the mandibular structure 6.Soft tissue of the midface around the nose moved anterior direction after mandibular setback surgery but there was no correlation between the amount of mandibular setback and the amount of the soft tissue changes

비강 및 부비동에 발생한 반전성 유두종의 임상-병리조직학적 고찰

노용훈,이승학,전세영,김용기,나한조,최봉남,서재홍 조선의대 1995 The Medical Journal of Chosun University Vol.20 No.2

人工湖를 利用한 淡水魚類(Blugill sunfish-Lepomis macrochirus macrochirus Rafiusesque)의 養殖에 關한 硏究

孫賢秀,曺鍾澤,韓熙世,裵泰雄 東亞大學校 1972 東亞論叢 Vol.9 No.-

本試驗은 九德貯水池의 環境要因 調査와 Blue gill sunfish의 粗放的 養殖試驗과 新農藥이 淡水魚類에 미치는 영향등을 調査하였던 바 얻어진 結果를 要約하면 다음과 같다. 試驗Ⅰ. 九德 貯水池의 環境要因 調査 1. 營養鹽과 水溫, pH, 透明度 等을 調査하여 試驗池의 生産力에 對하여 究明하였다. 2. 調査期間동안 水溫은 13.0℃∼27.0℃ 였으며 透明度는 40cm∼130cm, pH는 6.8∼8.0 溶存酸素 5.4cc/l∼7.9cc/l를 나타냈다. 3. 營養鹽에 있어서는 全窒素量과 全憐酸鹽은 plankton 生産에 充分한 量이 溶存되어 있으며 腐食性 營養湖에 해당되었다. 특히 규산염은 댜른 湖沼에 比해 대단히 높은 量的分布를 보였다. 4. phyto plankton은 17種이 出現하였으며 Ulthrix, Fragilaria, Desmipseud, Pediastrum 等이 量的 우세를 보였다. 5. Zooplankton은 Crustacea 幼生을 除外하고 15種이 出現하였으며, Cyclops Branchionus, Daphnia Eudiaptomus 等이 우세하였다. 試驗Ⅱ Blue gill sunfish의 粗放的 養殖試驗 1. Blue gill sunfish의 調査期間동안 增重量은 平均 7.64g 이었으며 잉어는 18.8g의 增重量을 나타냈다. 2. Blue gill의全長 및 體高의 比는 1 : 2.6∼2.8로써 餌料를 준 試料를 준 試驗과의 差異는 없었다. 3. 粗放的 養殖試驗의 結果 Blue gill sunfish는 二年魚가 飼育試驗한 1年魚의 約1/2에 가까운 增重量을나타내어 天然餌料만으로 飼育試驗으로는 좋은 結果를 보였다. 試驗Ⅲ 毒性調査 1. Blue gill sunfish와 잉어 稚魚에 대한 新農藥의 영향을 조사하여, 供試魚種間이 感受性의 差異에 대하여 比較檢討하였다. 2. Blue gill sunfish가 잉어에 비하여 感受性이 높은 農藥은 Diazinon乳劑, Birlane乳劑, Saturn-S粒劑등이 였고. 3. 잉어가 Blue gill sunifish보다 높은 感受性을 나타낸 것은 Kasugamin乳劑, Blasticidine-S乳劑, Sevin 水和劑, Kitazin乳劑, Lasso乳劑등이였다. 4. Blue gill sunfish의 養殖上 극히 注意를 要하는 毒性이 강한 農藥과 48時間後의 TLm値를 보면 Diazinon乳劑…0.08ppm, EPN乳劑-0.9ppm, Birlane乳劑…0.005ppm, Saturn-S粒劑…0.5ppm였다. The following are some results obtained from a series of research environmental factors and experiments in Blue gill sunfish culture, and effect of the new agricultural pesticides on fresh water fishes. Experiment Ⅰ. Research of environmental factors in Ku Dork Reservoir The work has been done as one of the basic study to productivity of Ku Doek Reservoir in Busan. The range of the water temperature was 13.0℃∼27.0℃ and maximum temperature was shown in late August. The values of the disolved oxygen were 5.4cc/l∼7.9cc/l and pH range was 6.8∼8.0, and the range of transparency was 40cm∼130cm. Total amounts of nitrogen ion and phosphorus ion were very abundant, moreover Sio?-Si amounts was higher concentration than other reservoirs. Phytoplankton was observed 17 species and dominant species were Ulthrix, Fragilaria, Desmipseud and Pediastrum. Zooplankton was observed 15 species except Crustacea larva and dominant species were Cyclops, Branchionus, Daphnia and Eudiaptomus. Total numbers of the phytoplankton and zooplankton were proportional to temperature. Experiment Ⅱ. Realing Blue gill sunfish. The work wa conducted by rearling Blue gill sunfish and Carp in Ku Doek Reservoir. Bluegill sunfishes body weight were increased 7.64g per one individual number and Carps were increased 18.8g during experiment period. A ratio relatived to total length(cm) and body depth(cm) were 1 : 2.6∼2.8 in this culture and no differnce was convinced between this culture and artifical culture. Experiment Ⅲ. Effect of the new agricultural pesticides on fresh water fish TLm velues of the new agricultural pesticides on Bluegill sun fish and Carp was investigated. Blue gill sunfish was more sensitive than Carp in Diazinon(E.C), Birlane(E.C), Saturn-S(G). Carp was more sensitive than Blue gill sunfish in Kasugamin(E.C), Blasticidin-s (E.C), Sevin(W.P), Kitazin(E.C), Lasso(E.C). Higher toxic pesticides on Blue gill sunfish were Diazinon(E.C), E.P.N(E.C), Birlane(E.C), Saturn-S(G), TLm values(after 48 hours) of these pesticides on Bluegill sunfish was as follows; Diazinon……0.8ppm, E.P.N……0.9ppm Birlane……0.005ppm, Saturm-S……0.5ppm.