튜브형 이산화질소 passive sampler의 태양광 영향에 관한 분석

이범진,김학민,인치경,김선태 대전대학교 산업기술연구소 2000 산업기술연구소 論文集 Vol.11 No.2

The purpose of this study is to estimate the effect of sunlight on the measurement results of the tube type of NO2 passive sampler. Total 252 samples; seven duplicates, two sites with different concentration level, and nine days concerning to both exposure to sunlight and screened passive samplers with aluminium foil, were measured and analyzed. Relative standard deviation(%) of measurement data in this study was calculated as less than 10%, and it meant that even the simplest passive sampler showed comparatively high precision. The average concentration values of the screened passive samplers were turned out to be 22.4%∼25.5% higher than that of the passive samplers which were exposed to sunlight. Consequently, the sunlight was found to be the most important meteorological factor to affect the measurement for the tube type of passive samplers.

바이오안전성에 대한 세계적인 동향 : 유전자변형 생물체(LMO)를 중심으로

이이,정찬문,이학수,신주식 충북대학교 연초연구소 2006 煙草硏究 Vol.20 No.-

현대 분자생물학의 발달에 따른 유전자변형 생물체의 개발은 시간과 경제적으로 전통적인 육종의 한계를 많이 극복하고 있으며 세계적으로 유전자변형생물체의 개발이 빠른 속도로 진행되고 있다. 유전자의 변형에 따른 환경의 파괴, 인체에 대한 위해 가능성 때문에 바이오안전성의정서가 채택되는 등 세계적으로 바이오안전성에 대한 관심이 증가하고 있다. 바이오안전성은 유전자특성분석, 환경위해성평가, 인체위해성평가 등 3단계의 평가에 의해서 이루어지며 최종적으로 인체와 환경에 해를 끼칠 가능성이 있는 유전자변형생물체를 규제하기 위해서 이루어진다. 우리나라도 최근에 바이오안전성에 대한 관심이 증대되면서 바이오안전성 평가체계가 확립중이다. The development of molecular biology enabled us to overcome the restrictions of conventional breeding system and the number of genetically modified organisms are dramatically increasing. The possibility of the genetically modified organism's risk on human health and environment caused concerns on the biosafety. Biosafety test contains gene characteristics analysis, health safety test, and environmental risk test. The final aim of these tests is to prevent the commercialization of genetically modified organism which have possible risk on human health and environment. Biosafety evaluation system is being built in Korea.

분자마커를 이용한 작물의 품종 개량과 품질관리

이이,엄유리,정찬문,석영선,이학수 충북대학교 연초연구소 2009 煙草硏究 Vol.22 No.-

분자마커는 현대 농업에서 없어서는 안 될 중요한 도구로 자리잡아가고 있으며 단백질과 DNA에 기초한 다양한 분자마커가 개발되었다. RFLP, RAPD, SSR, AFLP, CAPS, SNP, STS, DNA sequencing 등 다양한 분자마커는 독특한 특성을 가지고 있어서 용도에 맞게 분자마커를 선별하여 사용할 필요가 있다. 분자마커의 선별에서는 사용가능한 DNA의 양, DNA의 품질, 공우성 마커 여부, 비용 등을 고려해서 해야 한다. 담배에서는 주로 병저항성과 품종을 감별하기 위한 분자마커가 개발되어 이용되고 있으나 담배의 DNA 다형성이 다른 작물에 비하여 현저히 낮아 게놈프로젝트가 완성되면 resequencing을 이용하여 마커를 개발하는 것이 효율적일 것으로 보인다. Molecular marker is very important tool for modern agriculture. Various molecular markers were developed for detecting protein and DNA polymorphism. Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Simple Sequence Repeat (SSR), Amplified Fragment Length Polymorphism (AFLP), Cleaved Amplified Polymorphic Sequence (CAPS), Single Nucleotide Polymorphism (SNP), Sequence Tagged Site (STS) and DNA sequencing are typical molecular markers commonly used. Each molecular marker has its own specific characters. Researchers have to choose a molecular marker for their own demands with considering the DNA quantity required, DNA quality required, codominance, and cost for developing and analysis. In tobacco, disease resistance and cultivar identification are the main focus of marker development. DNA polymorphism of tobacco is much less than that of other crops. Completion of genome project followed by resequencing could be a good opportunity for molecular marker development of tobacco.

암모니아 공간분포 조사를 위한 Passive Sampler 개발에 관한 연구

김학민,이범진,김선태 대전대학교 환경문제연구소 2001 환경문제연구소 논문집 Vol.5 No.-

Passive and active samplers are widely deployed for measuring gaseous pollutants such as nitrogen dioxide, sulfur dioxide and ozone in urban and rural air, however odorous compounds like ammonia, hydrogen sulfide are rarely deployed, because they are easily changed by circumstance conditions and perceived that they need a large number of measuring sites to assess mean concentration over the area. So, it is considered that the odor related phenomena could not be easily defined by cost-effective and convenient method. In this study, we developed the new sampling tool for ammonia evaluation in ambient air and the results of evaluation for evaluate the suitability of developed passive sampler for the measurement of daily averaged ammonia concentrations and spatial distribution survey are presented. Three types of absorbing solutions, filters and caps were tested at laboratory condition and the conversion factor, precision and suitability of the samplers were derived from triplicated measurement at paper mill. It is defined that for absoubing ammonia, 2% of phosphoric acid and glycerine mix(1:1) solution and chromatography filter are suitable and rubber cap is unsuitable to use during sampling and storage period. The conversion factor was derived 7.0μA at ordinary ambient air by comparison with reference method(indo-phenol method) at three different conditions. The calculated results of standard deviation among the samplers were lower than 2ppb at 86% sampling sites of total 40 sites and we could recognize the ammonia sources and variation with change of wastewater treatment plant process. Therefore the developed passive samplers are appropriate to survey ammonia spatial distrivution.

제브라피쉬에서 장신경계에 의한 장운동 조절

이명호,문현이,권영숙,맹학영,김철희 충남대학교 생물공학연구소 2011 생물공학연구지 Vol.17 No.-

Structural performance evaluation of a steel-plate girder bridge using ambient acceleration measurements

Yi, Jin-Hak,Cho, Soojin,Koo, Ki-Young,Yun, Chung-Bang,Kim, Jeong-Tae,Lee, Chang-Geun,Lee, Won-Tae Techno-Press 2007 Smart Structures and Systems, An International Jou Vol.3 No.3

The load carrying capacity of a bridge needs to be properly assessed to operate the bridge safely and maintain it efficiently. For the evaluation of load carrying capacity considering the current state of a bridge, static and quasi-static loading tests with weight-controlled heavy trucks have been conventionally utilized. In these tests, the deflection (or strain) of the structural members loaded by the controlled vehicles are measured and analyzed. Using the measured data, deflection (or strain) correction factor and impact correction factor are calculated. These correction factors are used in the enhancement of the load carrying capacity of a bridge, reflecting the real state of a bridge. However, full or partial control of the traffic during the tests and difficulties during the installment of displacement transducers or strain gauges may cause not only inconvenience to the traffic but also the increase of the logistics cost and time. To overcome these difficulties, an alternative method is proposed using an excited response part of full measured ambient acceleration data by ordinary traffic on a bridge without traffic control. Based on the modal properties extracted from the ambient vibration data, the initial finite element (FE) model of a bridge can be updated to represent the current real state of a bridge. Using the updated FE model, the deflection of a bridge akin to the real value can be easily obtained without measuring the real deflection. Impact factors are obtained from pseudo-deflection, which is obtained by double-integration of the acceleration data with removal of the linear components on the acceleration data. For validation, a series of tests were carried out on a steel plategirder bridge of an expressway in Korea in four different seasons, and the evaluated load carrying capacities of the bridge by the proposed method are compared with the result obtained by the conventional load test method.

Evaluation of Vibration Characteristics of an Existing Harbor Caisson Structure Using Tugboat Impact Tests and Modal Analysis

Yi, Jin-Hak,Park, Woo-Sun,Lee, So-Young,Huynh, Thanh-Canh,Kim, Jeong-Tae,Seo, Chun-Kyo SAGE Publications 2013 International journal of distributed sensor networ Vol.2013 No.-

Numerical investigation on effects of rotor control strategy and wind data on optimal wind turbine blade shape

Yi, Jin-Hak,Yoon, Gil-Lim,Li, Ye Techno-Press 2014 Wind and Structures, An International Journal (WAS Vol.18 No.2

Recently, the horizontal axis rotor performance optimizer (HARP_Opt) tool was developed in the National Renewable Energy Laboratory, USA. This innovative tool is becoming more popular in the wind turbine industry and in the field of academic research. HARP_Optwas developed on the basis of two fundamental modules, namely, WT_Perf, a performance evaluator computer code using the blade element momentum theory; and a genetic algorithm module, which is used as an optimizer. A pattern search algorithm was more recently incorporated to enhance the optimization capability, especially the calculation time and consistency of the solutions. The blade optimization is an aspect that is highly dependent on experience and requires significant consideration on rotor control strategies, wind data, and generator type. In this study, the effects of rotor control strategies including fixed speed and fixed pitch, variable speed and fixed pitch, fixed speed and variable pitch, and variable speed and variable pitch algorithms on optimal blade shapes and rotor performance are investigated using optimized blade designs. The effects of environmental wind data and the objective functions used for optimization are also quantitatively evaluated using the HARP_Opt tool. Performance indices such as annual energy production, thrust, torque, and roof-flap moment forces are compared.

Characterization of Bacillus cereus SH-7 Extracellular Protease

Yi, Hak-Kyu,Chun, Young-Jin,Kim, Han-Bok The Microbiological Society of Korea 1999 The journal of microbiology Vol.37 No.4

An extracellular endopeptidase from Bacillus cereus SH-7 was purified to homogeneity. The protease was most active at pH 8 and $40{\circ}C$, respectively. The molecular mass of the protease was 40 kDa on SDS-PAGE, and 120 kDa by gel filtration, suggesting that the native enzyme is composed of three homogeneous subunits. The $K_m\;and\;V_{max}$ values of the protease for N-succinyl-$(Ala)_2$-Pro-Phe-p-nitroanilide were 11.11 mM and 170 nmol/mg of protein/min, respectively. The protease was also identified as a metalloprotease. The bioactivity of the SH-7 protease will need further study in the future.

Properties of Aluminum Doped Zinc Oxide Thin Film Prepared by Sol-gel Process

Yi, Sung-Hak,Kim, Jin-Yeol,Jung, Woo-Gwang Materials Research Society of Korea 2010 한국재료학회지 Vol.20 No.7

Transparent conducting aluminum-doped ZnO thin films were deposited using a sol-gel process. In this study, the important deposition parameters were investigated thoroughly to determine the appropriate procedures to grow large area thin films with low resistivity and high transparency at low cost for device applications. The doping concentration of aluminum was adjusted in a range from 1 to 4 mol% by controlling the precursor concentration. The annealing temperatures for the pre-heat treatment and post-heat treatment was $250^{\circ}C$ and 400-$600^{\circ}C$, respectively. The SEM images show that Al doped and undoped ZnO films were quite uniform and compact. The XRD pattern shows that the Al doped ZnO film has poorer crystallinity than the undoped films. The crystal quality of Al doped ZnO films was improved with an increase of the annealing temperature to $600^{\circ}C$. Although the structure of the aluminum doped ZnO films did not have a preferred orientation along the (002) plane, these films had high transmittance (> 87%) in the visible region. The absorption edge was observed at approximately 370 nm, and the absorption wavelength showed a blue-shift with increasing doping concentration. The ZnO films annealed at $500^{\circ}C$ showed the lowest resistivity at 1 mol% Al doping.