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      • SCOPUSKCI등재

        Preparation of AgCl/Ag<sub>3</sub>PO<sub>4</sub>/Diatomite Composite by Microemulsion Method for Rapid Photo-Degradation of Rhodamine B with Stability under Visible Light

        Zhu, Hai-Tao,Ren, Qi-Fang,Jin, Zhen,Ding, Yi,Liu, Xin-Yu,Ni, Xi-Hui,Han, Meng-Li,Ma, Shi-Yu,Ye, Qing,Oh, Won-Chun Materials Research Society of Korea 2020 한국재료학회지 Vol.30 No.8

        In this paper, AgCl/Ag<sub>3</sub>PO<sub>4</sub>/diatomite photocatalyst is successfully synthesized by microemulsion method and anion in situ substitution method. X-ray diffraction (XRD), photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), and ultraviolet-visible spectroscopy (UV-Vis) are used to study the structural and physicochemical characteristics of the AgCl/Ag<sub>3</sub>PO<sub>4</sub>/diatomite composite. Using rhodamine B (RhB) as a simulated pollutant, the photocatalytic activity and stability of the AgCl/Ag<sub>3</sub>PO<sub>4</sub>/diatomite composite under visible light are evaluated. In the AgCl/Ag<sub>3</sub>PO<sub>4</sub>/diatomite visible light system, RhB is nearly 100 % degraded within 15 minutes. And, after five cycles of operation, the photocatalytic activity of AgCl/Ag<sub>3</sub>PO<sub>4</sub>/diatomite remains at 95 % of the original level, much higher than that of pure Ag<sub>3</sub>PO<sub>4</sub> (40 %). In addition, the mechanism of enhanced catalytic performance is discussed. The high photocatalytic performance of AgCl/Ag<sub>3</sub>PO<sub>4</sub>/diatomite composites can be attributed to the synergistic effect of Ag<sub>3</sub>PO<sub>4</sub>, diatomite and AgCl nanoparticles. Free radical trapping experiments are used to show that holes and oxygen are the main active species. This material can quickly react with dye molecules adsorbed on the surface of diatomite to degrade RhB dye to CO<sub>2</sub> and H<sub>2</sub>O. Even more remarkably, AgCl/Ag<sub>3</sub>PO<sub>4</sub>/diatomite can maintain above 95 % photo-degradation activity after five cycles.

      • KCI등재

        Isolation and Characterization of a Novel Heterotrophic Nitrifying and Aerobic Denitrifying Bacterium Pseudomonas stutzeri KTB for Bioremediation of Wastewater

        Mao-hong Zhou,Hai-ren Ye,Xiao-wei Zhao 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.2

        A novel heterotrophic nitrifying and aerobicdenitrifying bacterium, KTB, was isolated from activatedsludge flocci collected from a biological aerated filteraccording to the modified Takaya method and identified asPseudomonas stutzeri by 16S rDNA gene sequence analysis. When shaking-cultured in the presence of 4.331 mmol/L ofnitrate, 4.511 mmol/L of nitrite and 4.438 mmol/L ofammonium, the strain grew fast, with μmax being 0.42, 0.45,and 0.56/h, and displayed high nitrogen removal efficiency,with nitrogen removal rate being 0.239, 0.362, and 0.361mmol/L/h and nitrogen removal ratio being 99.1, 100.0,and 100.0% in 18 h, respectively. The removal mainlyoccurred in the logarithmic phase. Nitrite accumulation didnot affect denitrification performance. Nitrate concentrationwas below the detectable limit during the whole growthcycle when ammonium was used as sole nitrogen source. It tolerated high DO level and exhibited excellent aggregationability. A possible pathway involved in the nitrogenremoval process, which demonstrated a full nitrificationand denitrification route, was speculated. The strain mightbe a great candidate for biological removal of nitrogencompounds from wastewater.

      • KCI등재

        Study on host plants for reproduction of Chilo suppressalis

        Wei-Hua Jiang,Hai-Dong Li,Xiong-Feng Cheng,Jian-Ren Ye,Yong-Bin Feng,Zhao-Jun Han 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3

        The rice stem borer, Chilo suppressalis, is generally considered to be a polyphagous pest. The current study challenges this view by investigating its oviposition preference, larval survival and development on different host plants under both laboratory and field conditions. Rice and water-oat populations of the borer inhabiting on corresponding plants respectively have partial reproductive isolation based on previous studies. In a laboratory multiple-choice test, C. suppressalis adults from rice population laid most of eggs on water-oat (46.5% of total eggs) and rice (43.8%), with very fewlaid onwheat (3.6%), sugarcane (4.0%) andmaize (2.0%). Field surveys supported the laboratory study and found no egg on the plants other than rice and water-oat. Neonate inoculation experiments performed in field and laboratory showed that larval survival rate was much higher on rice (49.1%–51.2%) and wheat (36.5%–44.1%) than that on water-oat (10.7%–10.8%), maize (1.2%–7.2%), sugarcane (0–1.5%) and weeds (2.4%). These results were discussed with the data reported from water-oat population and it was concluded that C. suppressalis is not a typical polyphagous pest. Rice population mainly reproduces on rice and use water-oat only as minor host, and water-oat population breeds better on water-oat than on rice as reported. Neither population could thrive on the other recorded host plants, which are used for the supplementary nutrition sources of larvae. These findings provide useful information for the development of control strategies to prevent C. suppressalis laying eggs on rice seedlings in early spring, hence effectively reducing population density of this pest in rice fields.

      • SCIESCOPUSKCI등재
      • KCI등재

        A κ-Carrageenase from a Newly Isolated Pseudoalteromonas-like Bacterium, WZUC10

        Mao-hong Zhou,Jian-she Ma,Jun Li,Hai-ren Ye,Ke-xin Huang,Xiao-wei Zhao 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5

        A bacterial strain able to produce k-carrageenase, designated WZUC10, was isolated from a live specimen of the red alga Plocamium telfairiae collected in the East China Sea. The phylogenetic evidence and phenotypic features indicate that this strain belongs to the genus Pseudoalteromonas. WZUC10 requires NaCl for growth and k-carrageenan to induce k-carrageenase synthesis; galactose and lactose do not induce it. The optimal growth temperature is 23~27°C. The secreted enzyme, which has a molecular mass of 45 kDa, breaks down k-carrageenan into k-neocarratetraose sulfate and larger oli-gosaccharides with a repeating β-D-Galp4S-(1→4)-α-D-AnGalp structure, but cannot degrade k-neocarratetraose sulfate or k-neocarrahexaose sulfate into k-neocarrabiose sulfate. The enzyme retains 90% of its activity after 2 h at 40°C and is com-pletely inactivated after 7.5 min at 70°C. The enzyme’s optimal temperature is 30°C and its optimal pH is 7.5. The enzyme-catalyzed reaction follows Michaelis-Menten kinetics, with the Michaelis constant (Km) and the turnover number (k) being 0.015 mM and 125 s-¹, respectively. WZUC10 produces 50 U/mL k-carrageenase after cultivation at 25°C for 35 h on a me-dium containing 80 g/L glucose, 5 g/L corn steep liquor, 3 g/L k-carrageenan, and 15 g/L NaCl. k-Neocarratetraose sulfate was prepared simply with precipitation by ethanol:water (5:1, v/v).

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