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      • KCI등재

        한국과 일본 대학생의 스포츠상황에 대한 도덕적 가치판단수준 비교

        함정혜(Chung Hae Hahm) 한국여성체육학회 2013 한국여성체육학회지 Vol.27 No.1

        The purpose of this study was to compare the differences of moral reasoning among general students, sports related majors students, and student athletes in Korea and Japan University. We collected total 612 out of 700 (315 from Korea, 297 from Japan) and 88 questionnaires were excluded questionnaire due to incompleteness and unreliable data. For this study, Hahm-Bellar Values Choice Inventory-16(HBVCI-16), which is a Moral Reasoning Questionnaire on Sports Activities, was used. The collected data was analyzed by t-test, one way ANOVA and two way ANOVA with using SPSS Var. 17.0 statistical package program and result were as follow. First, the Japan student`s overall score of moral reasoning were lower compare to Korean university students. Second, there were 1 % higher score of moral reasoning in woman against man and in younger subjects. Sports majored university students presented 1% higher score against other related students and higher grade students presented 5% higher score against lower grade students. Third, among student athletes of Korea and Japan, although there were no significant differences, it was 0.1% higher among those who engaged in individual sports and 5% higher score in those who have less sports career.

      • KCI등재

        A Model for Computer-Aided Process Planning System in Flexible Manufacturing Systems

        Kang, Young-Sig,Hahm, Hyo-Joon,Rim, Suk-Chul,Kim, Seung-Baum The Korean Society for Quality Management 1994 품질경영학회지 Vol.22 No.1

        Most of computer-aided process planning(CAPP) systems have been developed to automate the process planning function. In this paper, we describe an analytical model for a CAPP system in order to improve the performance of production system in flexible manufacturing systems(FMSs) for computer intergrated manufacturing(CIM) architecture. This paper proposes an optimal process planning that minimizes the load time by minimizing the cycle time and the number of workstations using Kang and Hahm's heuristic approach so as to improve the performance of production system under the batch production of discrete products. We also perform simulation using SIMAN language to campare the line utilization of each for various product types. The proposed algorithm can be implemented in existing FMSs for on-line control of product quantity using programmable logic controllers(PLC) and communication devices.

      • KCI등재
      • Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein

        Hahm, Young Hye,Hahm, Sung Ho,Jo, Hyoun Young,Ahn, Yeong Hee Hindawi 2018 International journal of analytical chemistry Vol.2018 No.-

        <P>Glycosylation is one of the most important posttranslational modifications for proteins, including therapeutic antibodies, and greatly influences protein physiochemical properties. In this study, glycopeptide mapping of a reference and biosimilar recombinant antibodies (rAbs) was performed using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and an automated Glycoproteome Analyzer (GPA) algorithm. The tandem mass analyses for the reference and biosimilar samples indicate that this approach proves to be highly efficient in reproducing consistent analytical results and discovering the implications of different rAb production methods on glycosylation patterns. Furthermore, the comparative analysis of a mutagenized rAb glycoprotein proved that a single amino acid mutation in the Fc portion of the antibody molecule caused increased variations in glycosylation patterns. These variations were also detected by the mass spectrometry method efficiently. This mapping method, focusing on precise glycopeptide identification and comparison for the identified glycoforms, can be useful in differentiating aberrant glycosylation in biosimilar rAb products.</P>

      • KCI등재
      • Spatial and temporal variation of net community production and its regulating factors in the Amundsen Sea, Antarctica

        Hahm, Doshik,Rhee, Tae Siek,Kim, Hae-Cheol,Park, Jisoo,Kim, Young-Nam,Shin, Hyoung Chul,Lee, SangHoon American Geophysical Union 2014 Journal of geophysical research. Oceans Vol.119 No.5

        We observed O2/Ar in the surface waters of the Amundsen Sea, Antarctica, during the austral summers in 2011 and 2012 to investigate the variability of net community production (NCP). Corresponding to the typical peak phytoplankton bloom period, the O2/Ar of the Amundsen Sea Polynya (ASP) reached 30% in early January 2011 and had a strong positive correlation with the sea surface temperature (SST) and chlorophyll-a (Chl-a). In contrast, O2/Ar decreased to -10% in the sea ice zone (SIZ), which was likely associated with either net O2 consumption in the unlit area or the entrainment of deep water containing low dissolved oxygen. Near the terminal stage of the phytoplankton bloom in late February 2012, we observed the same contrasting O2/Ar features between the ASP and SIZ. However, the O2/Ar in the ASP was not >10%, which corresponded with the overall reduction in Chl-a, solar radiation, and SST compared with the corresponding values in 2011. The average net community production in the ASP was 119 +/- 79 mmol O2 m-2 d-1 in January 2011, and 23 +/- 14 mmol O2 m-2 d-1 in February 2012. The strong correlations of NCP with SST and mixed layer depth (MLD) indicate that the ASP phytoplankton bloom is likely initiated by a combination of increased light availability and SST in early summer. Low SST and variable fluorescence to maximum florescence ratio (Fv/Fm) in February indicate that decreased solar radiation and Fe availability are likely responsible for the phytoplankton bloom demise.

      • SCIESCOPUSKCI등재

        In Vitro Formation of Active Carboxypeptidase Y from Pro-Carboxypeptidase Y Inclusion Bodies by Fed-Batch Operation

        HAHM, MOON SUN,CHUNG, BONG HYUN 한국미생물 · 생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.5

        The gene encoding yeast pro-carboxypeptidase Y (pro-CPY) has been cloned and expressed in Escherichia coli. Most of the expressed pro-CPY was accumulated as cytoplasmic insoluble aggregates. In our previous study [3], active CPY was obtained by renaturation of entirely denatured pro-CPY followed by in vitro proteolytic processing with proteinase K along with the activation process. The same refolding process was performed to produce an active CPY from pro-CPY inclusion bodies with renaturation buffers containing proteinase K at different concentrations. The refolding efficiency decreased from 25% to 2% in the renaturation buffers containing proteinase K at concentrations of 60㎍/ml and 0.6㎍/ml, respectively. In an attempt to increase the refolding efficiency with a lesser amount of proteinase K, a novel fed-batch refolding process was developed. In a fed-batch refolding, 99 ml of the renaturation buffer containing pro-CPY was gradually added into I ml of the renaturation buffer containing 60 ㎍/ml of proteinase K to give a final proteinase K concentration of 0.6 ㎍/ml. The fed-batch refolding process resulted in a refolding efficiency of 18%, which corresponded to a 9-fold increase over that (2%) in the batch process.

      • KCI등재

        Characterization of the Nickel Resistance Gene from Legionella pneumophila : Attenuation of Nickel Resistance by ppk(polyphosphate kinase) Disruption in Escherichia coli

        Hahm, Dae Hyun,Yeom, Mi Jung,Ko, Whae Min,Lee, Eunjoo H.,Lee, Hye Jung,Shim, In Sop,Kim, Hong Yeoul 한국미생물 · 생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.1

        A 1,989-bp genomic region encoding nickel resistance genes was isolated from Legionella pneumophila, a pathogen for legionellosis. From a sequencing and computer analysis, the region was found to harbor two structural genes, a nreB-like protein gene (1,149 bp) and a nreA-like protein gene (270 bp), in a row. Both genes exhibited a significant degree of similarity to the corresponding genes from Synechocystis sp. PCC6803 (54% amino acid sequence identity) and Achromobacter xylosoxidans 31A (76%). The gene was successfully expressed in E. coli MG1655 and conferred a nickel resistance of up to 5mM in an LB medium and 3 mM in a TMS medium including gluconate as the sole carbon source. E. coli harboring the nickel resistance gene also exhibited a substantial resistance to cobalt, yet no resistance to cadmium or zinc. Since the extracellular concentration of nickel remained constant during the whole period of cultivation, it was confirmed that the nickel resistance was provided by an efflux system like the Ni^2+ permease (nrsD) of Synechocystis sp. strain PCC6803. Since polyphosphate (poly-P) is known as a global regulator for gene expression as well as a potential virulence factor in E. coli, the nickel resistance of a ppk mutant of E. coli MG1655 harboring the nickel resistance gene from L. pneumophila was compared with that of its parental strain. The nickel resistance was significantly attenuated by ppk inactivation, which was more pronounced in an LB medium than in a TMS medium.

      • SCISCIESCOPUS

        An overview of the volatile systematics of the Lau Basin – Resolving the effects of source variation, magmatic degassing and crustal contamination

        Hahm, Doshik,Hilton, David R.,Castillo, Paterno R.,Hawkins, James W.,Hanan, Barry B.,Hauri, Erik H. Elsevier 2012 Geochimica et cosmochimica acta Vol.85 No.-

        <P><B>Abstract</B></P><P>The Lau Basin erupts lavas with a range of geochemical features reflecting a complex history of interaction involving different mantle sources. The Valu Fa Ridge (VFR) and Mangatolu Triple Junction (MTJ) region have lavas with arc-like characteristics, Niuafo’ou Island (NV), Peggy Ridge and Central and Eastern Lau Spreading Centers (PR, CLSC and ELSC) erupt mid-ocean ridge basalt (MORB)-like volcanics, whereas the Rochambeau Bank (RB) has features akin to ocean island basalt (OIB). To characterize the volatile systematics of these various regions, we report a comprehensive study of 39 submarine lavas from these various eruptive centers encompassing analyses of the noble gases (He, Ne, and Ar) and carbon (CO<SUB>2</SUB>) – both isotopes and abundances – together with other major volatile phases (H<SUB>2</SUB>O, S, Cl, and F).</P><P>Helium isotope ratios of the NV, MTJ, CLSC, and ELSC are MORB-like for the most part except for differentiated lavas that tend to have lower, more radiogenic <SUP>3</SUP>He/<SUP>4</SUP>He values. The RB has considerably higher <SUP>3</SUP>He/<SUP>4</SUP>He ratios (up to 23 <I>R</I><SUB>A</SUB> in this work) which extend as far south as the PR. The influence of ‘plume-like’ sources in the RB is also apparent in Ne isotopes: RB samples follow a trend similar to Hawaiian basalts in 3-isotope neon space. However, RB lavas have lower <SUP>40</SUP>Ar/<SUP>36</SUP>Ar (300–730) and higher [<SUP>36</SUP>Ar] than CLSC and ELSC, suggesting greater air contamination. Elemental He/Ne ratios (<SUP>3</SUP>He/<SUP>22</SUP>Ne<SUB>S</SUB> and <SUP>4</SUP>He/<SUP>21</SUP>Ne<SUP>∗</SUP> where S=solar and *=nucleogenic) are high throughout the Lau Basin and identify the Lau mantle as one of only two high <SUP>3</SUP>He/<SUP>4</SUP>He provinces worldwide with such an enrichment of He relative to Ne.</P><P>Magmatic CO<SUB>2</SUB> and δ<SUP>13</SUP>C fall in the range 7–350ppm and −28‰ to −6‰, respectively. RB lavas have less [CO<SUB>2</SUB>] and slightly lower δ<SUP>13</SUP>C than CLSC and ELSC. The lowest values are found among MTJ lavas. These lavas also have the highest [H<SUB>2</SUB>O], [F], [Cl], and [S] whereas the PR, ELSC and CLSC have the lowest. RB has intermediate [H<SUB>2</SUB>O]. We estimate primary [CO<SUB>2</SUB>] in primary melts using [CO<SUB>2</SUB>]–δ<SUP>13</SUP>C relationships, and find that RB lavas have higher [CO<SUB>2</SUB>] (∼935±168ppm) than ELSC/CLSC (638±115ppm). They also possess higher initial δ<SUP>13</SUP>C values, consistent with observations at other hotspot-related localities. However, there are no discernible differences in primary CO<SUB>2</SUB>/Nb ratios between mantle sources characterized by high <SUP>3</SUP>He/<SUP>4</SUP>He and MORB-like ratios. On the other hand, reconstructed values are considerably higher than that envisaged for depleted MORB mantle based on olivine-hosted melt inclusions.</P>

      • Design Principle for Bright, Robust, and Color-Pure InP/ZnSe<i><sub>x</sub></i>S<sub>1-<i>x</i></sub>/ZnS Heterostructures

        Hahm, Donghyo,Chang, Jun Hyuk,Jeong, Byeong Guk,Park, Philip,Kim, Jaeyoul,Lee, Seongjae,Choi, Jongha,Kim, Whi Dong,Rhee, Seunghyun,Lim, Jaehoon,Lee, Doh C.,Lee, Changhee,Char, Kookheon,Bae, Wan Ki American Chemical Society 2019 Chemistry of materials Vol.31 No.9

        <P>Advance in wet chemistry enables the sophisticated design of nanocrystal quantum dots (QDs) and allows unprecedented color purity and brightness, promising their useful applications in a variety of light-emitting applications. A representative example is core/shell heterostructures, in which charge carriers are effectively decoupled from structural artifacts to generate photons efficiently. Despite the development of widely accepted synthetic protocols for Cd- or Pb-based QDs, the progress in heterostructuring environmentally benign QDs has been lagging behind, and so is the practical use of these QDs. Herein, we present a design principle for InP/ZnSe<I><SUB>x</SUB></I>S<SUB>1-<I>x</I></SUB> heterostructured QDs. A principal design approach is the growth of uniformly thick inorganic shell consisting of a ZnSe<I><SUB>x</SUB></I>S<SUB>1-<I>x</I></SUB> inner shell and a ZnS outermost shell that effectively confines electrons from spreading inward of QDs. Comprehensive studies across synthesis, spectroscopic analysis, and calculation uncover that the presence of Se near the InP emissive core enables a uniform shell growth to an extended thickness and the S-rich exterior shell ensures the decoupling of the electron wave function from the surface trap states. Engineering composition profile across multiple shells enables us to realize InP/thick-shell QDs meeting the requirements of light-emitting applications such as high photoluminescence quantum yield, narrow spectral bandwidth, and enhanced photochemical robustness. We capitalize on bright, robust, and color-pure InP/ZnSe<I><SUB>x</SUB></I>S<SUB>1-<I>x</I></SUB>/ZnS QDs with a range of emission wavelength covering from cyan to red regions by exemplifying their use in the primary-color light-emitting diodes (peak external quantum efficiency of 3.78 and 3.92% for green- and red-emitting ones, respectively).</P> [FIG OMISSION]</BR>

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