Suppression of miRNA-708 by Polycomb Group Promotes Metastases by Calcium-Induced Cell Migration

Ryu, S.,McDonnell, K.,Choi, H.,Gao, D.,Hahn, M.,Joshi, N.,Park, S.M.,Catena, R.,Do, Y.,Brazin, J.,Vahdat, Linda T.,Silver, Randi B.,Mittal, V. Cell Press 2013 Cancer cell Vol.23 No.1

The progression of cancer to metastatic disease is a major cause of death. We identified miR-708 being transcriptionally repressed by polycomb repressor complex 2-induced H3K27 trimethylation in metastatic breast cancer. miR-708 targets the endoplasmic reticulum protein neuronatin to decrease intracellular calcium level, resulting in reduction of activation of ERK and FAK, decreased cell migration, and impaired metastases. Ectopic expression of neuronatin refractory to suppression by miR-708 rescued cell migration and metastasis defects. In patients with breast cancer, miR-708 expression was decreased in lymph node and distal metastases, suggesting a metastasis-suppressive role. Our findings uncover a mechanistic role for miR-708 in metastasis and provide a rationale for developing miR-708 as a therapeutic agent against metastatic breast cancer.

Cloning and characterization of a thermostable H₂O-forming NADH oxidase from Lactobacillus rhamnosus

Zhang, Y.W.,Tiwari, M.K.,Gao, H.,Dhiman, S.S.,Jeya, M.,Lee, J.K. IPC Science and Technology Press ; Elsevier Scienc 2012 Enzyme and microbial technology Vol.50 No.4

NADH oxidase (Nox) catalyzes the conversion of NADH to NAD<SUP>+</SUP>. A previously uncharacterized Nox gene (LrNox) was cloned from Lactobacillus rhamnosus and overexpressed in Escherichia coli BL21(DE3). Sequence analysis revealed an open reading frame of 1359bp, capable of encoding a polypeptide of 453 amino acid residues. The molecular mass of the purified LrNox enzyme was estimated to be ∼50kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 100kDa by gel filtration chromatography, suggesting that the enzyme is a homodimer. The enzyme had optimal activity at pH 5.6 and temperature 65<SUP>o</SUP>C, and k<SUB>cat</SUB>/K<SUB>m</SUB> of 3.77x10<SUP>7</SUP>s<SUP>-1</SUP>M<SUP>-1</SUP>, the highest ever reported. Heat inactivation studies revealed that LrNox had high thermostability, with a half-life of 120min at 80<SUP>o</SUP>C. Molecular dynamics simulation studies shed light on the factors contributing to the high activity of LrNox. Although the properties of Nox from several microorganisms have been reported, this is the first report on the characterization of a recombinant H<SUB>2</SUB>O-forming Nox with high activity and thermostability. The characteristics of the LrNox enzyme could prove to be of interest in industrial applications such as NAD<SUP>+</SUP> regeneration.

Taxonomy of fungal complex causing red-skin root of Panax ginseng in China

Xiao H. Lu,Xi M. Zhang,Xiao L. Jiao,Jianjun J. Hao,Xue S. Zhang,Yi Luo,Wei W. Gao 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.3

Background: Red-skin root of Asian ginseng (Panax ginseng) significantly reduces the quality and limits theproduction of ginseng in China. The disease has long been thought to be a noninfectious physiologicaldisease, except one report that proved itwas an infectious disease. However, the causal agents have not beensuccessfully determined. In the present study, we were to reveal the pathogens that cause red-skin disease. Methods: Ginseng roots with red-skin root symptoms were collected from commercial fields in NortheastChina. Fungi were isolated from the lesion and identified based on morphological characters alongwith multilocus sequence analyses on internal transcription spacer, b-tubulin (tub2), histone H3 (his3),and translation elongation factor 1a (tef-1a). Pathogens were confirmed by inoculating the isolates inginseng roots. Results: A total of 230 isolates were obtained from 209 disease samples. These isolates were classifiedinto 12 species, including Dactylonectria sp., D. hordeicola, Fusarium acuminatum, F. avenaceum, F. solani,F. torulosum, Ilyonectria mors-panacis, I. robusta, Rhexocercosporidium panacis, and three novel speciesI. changbaiensis, I. communis, and I. qitaiheensis. Among them, I. communis, I. robusta, and F. solani had thehighest isolation frequencies, being 36.1%, 20.9%, and 23.9%, respectively. All these species isolated werepathogenic to ginseng roots and caused red-skin root disease under appropriate condition. Conclusion: Fungal complex is the causal agent of red-skin root in P. ginseng.

Taxonomy of fungal complex causing red-skin root of Panax ginseng in China

Lu, Xiao H.,Zhang, Xi M.,Jiao, Xiao L.,Hao, Jianjun J.,Zhang, Xue S.,Luo, Yi,Gao, Wei W. The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.3

Background: Red-skin root of Asian ginseng (Panax ginseng) significantly reduces the quality and limits the production of ginseng in China. The disease has long been thought to be a noninfectious physiological disease, except one report that proved it was an infectious disease. However, the causal agents have not been successfully determined. In the present study, we were to reveal the pathogens that cause red-skin disease. Methods: Ginseng roots with red-skin root symptoms were collected from commercial fields in Northeast China. Fungi were isolated from the lesion and identified based on morphological characters along with multilocus sequence analyses on internal transcription spacer, β-tubulin (tub2), histone H3 (his3), and translation elongation factor 1α (tef-1α). Pathogens were confirmed by inoculating the isolates in ginseng roots. Results: A total of 230 isolates were obtained from 209 disease samples. These isolates were classified into 12 species, including Dactylonectria sp., D. hordeicola, Fusarium acuminatum, F. avenaceum, F. solani, F. torulosum, Ilyonectria mors-panacis, I. robusta, Rhexocercosporidium panacis, and three novel species I. changbaiensis, I. communis, and I. qitaiheensis. Among them, I. communis, I. robusta, and F. solani had the highest isolation frequencies, being 36.1%, 20.9%, and 23.9%, respectively. All these species isolated were pathogenic to ginseng roots and caused red-skin root disease under appropriate condition. Conclusion: Fungal complex is the causal agent of red-skin root in P. ginseng.

Malic Acid 혹은 Citric acid 의 첨가가 반추위 혼합미생물의 성장 및 대사에 미치는 영향

고주,이성실,노영애,김홍대,하종규,감동근,전현식 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.3

본 연구는 malic acid와 citric acid의 첨가가 반추위 혼합미생물의 성장 및 대사에 미치는 영향을 구명하기 위해서 실시되었는데, 시험결과를 요약하면 다음과 같다. Malic acid와 citric acid를 각각 혼합미생물의 배지에 첨가했을 때 배양 12∼24시간에서 두 처리구에서 모두 박테리아의 성장이 크게 촉진되었어며 citric aicd의 첨가효과가 malic acid보다 더 좋았고 박테리아중 amylase를 분비하는 박테리아가 주로 많이 성장하였다. 그리고 곰팡이는 12∼24시간까지는 malic acid와 citric acid 첨가구에서 모두 대조구에 비하여 유의성있게 증가하다 48시간 후에는 급격히 감소하였다. 따라서 섬유소 분해효소의 활력도 48시간부터 급격히 감소하는 것으로 나타났다. This study was conducted to evaluate the effects of malic or citric acid on the growth and cellulolytic activity of mixed rumen microbes. The mixed rumen microbes were inoculated into Lowe's medium containing 0.01% malic or citric acid, and incubated for 12, 24, 48, 72, 120 and 168 hours. After incubation, enzyme (CMCase, xylanase, amylase and protease) activity, microbial growth and gas production were estimated. Results were obtained as below Bacterial growth rate and amylase activity were increased by addition of 0.01% malic acid or citric acid at 12 and 24 hour after incubation, and this stimulatory effect was more obvious with citric acid than with malic acid. Malic or citric acid addition increased fungal growth and cellulolytic enzyme activity upto 24 hour incubation.

A first principles study of NO₂ chemisorption on silicon carbide nanotubes

Gao, G.,Park, S.H.,Kang, H.S. Elsevier Science Publishers [etc.] 2009 Chemical physics Vol.355 No.1

Using methods based on first principles, we find that an NO<SUB>2</SUB> molecules can be chemisorbed on silicon carbide nanotubes (SiCNTs) with an appreciable binding energy (∼-1.0eV), and that this is not the case for either carbon nanotubes (CNTs) or boron nitride nanotubes (BNNTs). A detailed analysis of the energetics, geometry, and electronic structure of various isomers of the complexes was performed. The SiCNT-NO<SUB>2</SUB> complex can be metallic or nonmetallic depending on the type of adsorption site and the chirality of the tube. However, our analysis of the electronic structure predicts that a strong p-type effect of the adsorption turns semiconducting systems into metallic ones at room temperature, irrespective of the chirality of the tube.

Identification, isolation and expression analysis of eight stress-related R2R3-MYB genes in tartary buckwheat (Fagopyrum tataricum)

Gao, F.,Zhao, H. X.,Yao, H. P.,Li, C. L.,Chen, H.,Wang, A. H.,Park, S. U.,Wu, Q. Springer Science + Business Media 2016 Plant cell reports Vol.35 No.6

<P>Eight R2R3 - MYB genes in tartary buckwheat were identified, and their expression patterns were comprehensively analyzed, which reveals role in plant response to abiotic stresses. The proteins of the R2R3-MYB superfamily play key roles in the growth and development processes as well as defense responses in plants. However, their characteristics and functions have not been fully investigated in tartary buckwheat (Fagopyrum tataricum), a strongly abiotic resistant coarse cereal. In this article, eight tartary buckwheat R2R3-MYB genes were isolated with full-length cDNA and DNA sequences. Phylogenetic analysis of the members of the R2R3-MYB superfamily between Arabidopsis and tartary buckwheat revealed that the assumed functions of the eight tartary buckwheat R2R3-MYB proteins are divided into five Arabidopsis functional subgroups that are involved in abiotic stress. Expression analysis during abiotic stress and exogenous phytohormone treatments identified that the eight R2R3-MYB genes responded to one or more treatments. This study is the first comprehensive analysis of the R2R3-MYB gene family in tartary buckwheat under abiotic stress.</P>

Characterization of FSW Butt Joints of Aluminum-clad Aluminum Thin Sheets

S. Basak(바삭 소우미야브라타),M. Mondal(몬달 무나리크),K. Gao(가오 쿤),S. Y. Anaman(아나만 샘 요),H.-H. Cho(훈휴 조),S.-T. Hong(홍성태) Korean Society for Precision Engineering 2021 한국정밀공학회 학술발표대회 논문집 Vol.2021 No.5월

Stromal fibroblasts from the interface zone of human breast carcinomas induce an epithelial-mesenchymal transition-like state in breast cancer cells in vitro

Gao, M.-Q.,Kim, B.G.,Kang, S.,Choi, Y.P.,Park, H.,Kang, K.S.,Cho, N.H. COMPANY OF BIOLOGIST LTD 2010 Journal of cell science Vol.123 No.20

Malic acid 와 Citric acid 의 첨가가 반추위 곰팡이의 성장 및 섬유소 분해율에 미치는 영향

고주,이성실,노영애,김홍대,하종규,양승학 한국영양사료학회 1999 韓國營養飼料學會誌 Vol.23 No.3

본 실험은 처음으로 malic acid와 citric acid의 첨가가 곰팡이의 성장 및 섬유소 분해력에 미치는 영향을 관찰하였고 혼합 미생물의 성장 및 대사에 미치는 영향을 구명하고자 실험 1, 2, 3으로 나누어 수행하였는데, malic acid의 농도가 배지의 0.001%∼0.01%까지는 곰팡이의 성장 및 섬유소 분해효소의 활력과 섬유소 분해율을 증가시켰다. 그러나 0.1% 이상의 malic acid를 첨가하면 오히려 곰팡이의 성장이나 섬유소 분해효소 및 섬유소 분해력이 떨어지는 것으로 나타났다. 그리고 citic acid를 배지의 0.005% 첨가했을 때 곰팡이의 성장량과 섬유소 분해효소의 활력 및 섬유소 분해율이 대조구에 비하여 유의적으로 증가하였으며 0.1%에서는 크게 억제되는 것으로 나타났다 This study was conducted to evaluate the effects of malic or citric acid supplementation on the growth and cellulolytic activity of rumen fungi. Malic acid or citric acid were supplemented into Lowe's medium at the level of 0.001, 0.005, 0.01, 0.05 and 0.1%, respectively. N. frontalis was used as inoculant. Incubation was lasted for 1, 2, 3, 5 and 7 days and pH, CMCase and xylanase activity, filter paper degradability and fungal protein were estimated after incubation. When supplementation level was from 0.001 to 0.01%, malic acid stimulated the fungal growth, cellulolytic enzymes activity and cellulose degradability, but results were reversed when malic acid concentration was above 0.1%. Citric acid affected rumen fungal growth and cellulolytic enzyme activity in the similar trend. .