Saccharomyces cerevisiae Live Cells Decreased In vitro Methane Production in Intestinal Content of Pigs

Gong, Y.L.,Liao, X.D.,Liang, J.B.,Jahromi, M.F.,Wang, H.,Cao, Z.,Wu, Y.B. Asian Australasian Association of Animal Productio 2013 Animal Bioscience Vol.26 No.6

An in vitro gas production technique was used in this study to elucidate the effect of two strains of active live yeast on methane ($CH_4$) production in the large intestinal content of pigs to provide an insight to whether active live yeast could suppress $CH_4$ production in the hindgut of pigs. Treatments used in this study include blank (no substrate and no live yeast cells), control (no live yeast cells) and yeast (YST) supplementation groups (supplemented with live yeast cells, YST1 or YST2). The yeast cultures contained $1.8{\times}10^{10}$ cells per g, which were added at the rates of 0.2 mg and 0.4 mg per ml of the fermented inoculum. Large intestinal contents were collected from 2 Duroc${\times}$Landrace${\times}$Yorkshire pigs, mixed with a phosphate buffer (1:2), and incubated anaerobically at $39^{\circ}C$ for 24 h using 500 mg substrate (dry matter (DM) basis). Total gas and $CH_4$ production decreased (p<0.05) with supplementation of yeast. The methane production reduction potential (MRP) was calculated by assuming net methane concentration for the control as 100%. The MRP of yeast 2 was more than 25%. Compared with the control group, in vitro DM digestibility (IVDMD) and total volatile fatty acids (VFA) concentration increased (p<0.05) in 0.4 mg/ml YST1 and 0.2 mg/ml YST2 supplementation groups. Proportion of propionate, butyrate and valerate increased (p<0.05), but that of acetate decreased (p<0.05), which led to a decreased (p<0.05) acetate: propionate (A: P) ratio in the both YST2 treatments and the 0.4 mg/ml YST 1 supplementation groups. Hydrogen recovery decreased (p<0.05) with yeast supplementation. Quantity of methanogenic archaea per milliliter of inoculum decreased (p<0.05) with yeast supplementation after 24 h of incubation. Our results suggest that live yeast cells suppressed in vitro $CH_4$ production when inoculated into the large intestinal contents of pigs and shifted the fermentation pattern to favor propionate production together with an increased population of acetogenic bacteria, both of which serve as a competitive pathway for the available H2 resulting in the reduction of methanogenic archaea.

China Spallation Neutron Source: Accelerator Design Iterations and R&D Status

J. Wei,C.-D. Deng,C.-H. Wang,C.-T. Shi,H. Sun,H.-F. Ouyang,H.-M. Qu,H.-Y. Dong,J. Li,J. Zhang,J.-S. Cao,J.-Y. Tang,L. Dong,L.-L. Wang,Q. Qin,Q.-B. Wang,S. Wang,S.-N. Fu,S.-X Fang,T. -G. Xu,W. Kang,Y.- 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.I

The China Spallation Neutron Source (CSNS) is a high-power, accelerator-based project currently under preparation. The accelerator complex consists of an H$^-$ ion source, an H$^-$ linac, a rapid-cycling proton synchrotron, and the transport lines. During the past year, the design of most accelerator systems went through major iterations, and initial research and developments was started on the prototyping of several key components.

Effects of turbulence intensity and exterior geometry on across-wind aerodynamic damping of rectangular super-tall buildings

Y. Quan,H.L. Cao,M. Gu 한국풍공학회 2016 Wind and Structures, An International Journal (WAS Vol.22 No.2

Across-wind aerodynamic damping ratios are identified from the wind-induced acceleration responses of 15 aeroelastic models of rectangular super-high-rise buildings in various simulated wind conditions by using the random decrement technique. The influences of amplitude-dependent structural damping ratio and natural frequency on the estimation of the aerodynamic damping ratio are discussed and the identifying method for aerodynamic damping is improved at first. Based on these works, effects of turbulence intensity Iu, aspect ratio H/B, and side ratio B/D on the across-wind aerodynamic damping ratio are investigated. The results indicate that turbulence intensity and side ratio are the most important factors that affect across-wind aerodynamic damping ratio, whereas aspect ratio indirectly affects the aerodynamic damping ratio by changing the response amplitude. Furthermore, empirical aerodynamic damping functions are proposed to estimate aerodynamic damping ratios at low and high reduced speeds for rectangular super-high-rise buildings with an aspect ratio in the range of 5 to 10, a side ratio of 1/3 to 3, and turbulence intensity varying from 1.7% to 25%.

Effects of turbulence intensity and exterior geometry on across-wind aerodynamic damping of rectangular super-tall buildings

Quan, Y.,Cao, H.L.,Gu, M. Techno-Press 2016 Wind and Structures, An International Journal (WAS Vol.22 No.2

Across-wind aerodynamic damping ratios are identified from the wind-induced acceleration responses of 15 aeroelastic models of rectangular super-high-rise buildings in various simulated wind conditions by using the random decrement technique. The influences of amplitude-dependent structural damping ratio and natural frequency on the estimation of the aerodynamic damping ratio are discussed and the identifying method for aerodynamic damping is improved at first. Based on these works, effects of turbulence intensity $I_u$, aspect ratio H/B, and side ratio B/D on the across-wind aerodynamic damping ratio are investigated. The results indicate that turbulence intensity and side ratio are the most important factors that affect across-wind aerodynamic damping ratio, whereas aspect ratio indirectly affects the aerodynamic damping ratio by changing the response amplitude. Furthermore, empirical aerodynamic damping functions are proposed to estimate aerodynamic damping ratios at low and high reduced speeds for rectangular super-high-rise buildings with an aspect ratio in the range of 5 to 10, a side ratio of 1/3 to 3, and turbulence intensity varying from 1.7% to 25%.

Improvement of lower hybrid current drive systems for high-power and long-pulse operation on EAST

Wang M.,Liu L.,Zhao L.M.,Li M.H.,Ma W.D.,Hu H.C.,Wu Z.G.,Feng J.Q.,Yang Y.,Zhu L.,Chen M.,Zhou T.A.,Jia H.,Zhang J.,Cao L.,Zhang L.,Liang R.R.,Ding B.J.,Zhang X.J.,Shan J.F.,Liu F.K.,Ekedahl A.,Gonich 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.11

Aiming at high-power and long-pulse operation up to 1000 s, some improvements have been made for both 2.45 GHz and 4.6 GHz lower hybrid (LH) systems during the recent 5 years. At first, the guard limiters of the LH antennas with graphite tiles were upgraded to tungsten, the most promising material for plasma facing components in nuclear fusion devices. These new guard limiters can operate at a peak power density of 12.9 MW/m2 . Strong hot spots were usually observed on the old graphite limiters when 4.6 GHz system operated with power >2.0 MW [B. N. Wan et al., Nucl. Fusion 57 (2017) 102019], leading to a reduction of the maximum power capability. With the new limiters, 4.6 GHz LH system, the main current drive (CD) and electron heating tool for EAST, can be operated with power >2.5 MW routinely. Long-pulse operation up to 100 s with 4.6 GHz LH power of 2.4 MW was achieved in 2021 and the maximal temperature on the guard limiters measured by an infrared (IR) camera was about 540 C, much below the permissible value of tungsten material (~1200 C). A discharge with a duration of 1056 s was achieved and the 4.6 GHz LH energy injected into the plasma was up to 1.05 GJ. Secondly, the fully-activemultijunction (FAM) launcher of 2.45 GHz system was upgraded to a passive-active-multijunction (PAM), for which the density of optimum coupling was relatively low (below the cut-off value). Good coupling with reflection coefficient ~3% has been achieved with plasma-antenna distance up to 11 cm for the new PAM. Finally, in order to eliminate the effect of ion cyclotron range of frequencies (ICRF) wave on 4.6 GHz LH wave coupling, the location of the ICRF launcher was changed to a port that is located 157.5 toroidally from the 4.6 GHz LH system and is not magnetically connected

Gene Microarray Analysis for Porcine Adipose Tissue: Comparison of Gene Expression between Chinese Xiang Pig and Large White

Guo, W.,Wang, S.H.,Cao, H.J.,Xu, K.,Zhang, J.,Du, Z.L.,Lu, W.,Feng, J.D.,Li, N.,Wu, C.H.,Zhang, L. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.1

We created a cDNA microarray representing approximately 3,500 pig genes for functional genomic studies. The array elements were selected from 6,494 cDNA clones identified in a large-scale expressed sequence tag (EST) project. These cDNA clones came from normalized and subtracted porcine adipose tissue cDNA libraries. Sequence similarity searches of the 3,426 ESTs represented on the array using BLASTN identified 2,790 (81.4%) as putative human orthologs, with the remainder consisting of "novel" genes or highly divergent orthologs. We used the gene microarray to profile transcripts expressed by adipose tissue of fatty Chinese Xiang pig (XP) and muscley Large White (LW). Microarray analysis of RNA extracted from adipose tissue of fatty XP and muscley LW identified 81 genes that were differently expressed two fold or more. Transcriptional differences of four of these genes, adipocyte fatty acid binding protein (aP2), stearyl-CoA desaturase (SCD), sterol regulatory element binding transcription factor 1 (SREBF1) and lipoprotein lipase (LPL) were confirmed using SYBR Green quantitative RT-PCR technology. Our results showed that high expression of SCD and SREBF1 may be one of the reasons that larger fat deposits are observed in the XP. In addition, our findings also illustrate the potential power of microarrays for understanding the molecular mechanisms of porcine development, disease resistance, nutrition, fertility and production traits.

Buckling of axial compressed cylindrical shells with stepwise variable thickness

H.G. Fan,Z.P. Chen,W.Z. Feng,F. Zhou,X.L. Shen,G.W. Cao 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.54 No.1

This paper focuses on an analytical research on the critical buckling load of cylindrical shells with stepwise variable wall thickness under axial compression. An arctan function is established to describe the thickness variation along the axial direction of this kind of cylindrical shells accurately. By using the methods of separation of variables, small parameter perturbation and Fourier series expansion, analytical formulas of the critical buckling load of cylindrical shells with arbitrary axisymmetric thickness variation under axial compression are derived. The analysis is based on the thin shell theory. Analytic results show that the critical buckling load of the uniform shell with constant thickness obtained from this paper is identical with the classical solution. Two important cases of thickness variation pattern are also investigated with these analytical formulas and the results coincide well with those obtained from other authors. The cylindrical shells with stepwise variable wall thickness, which are widely used in actual engineering, are studied by this method and the analytical formulas of critical buckling load under axial compression are obtained. Furthermore, an example is presented to illustrate the effects of each strake’s length and thickness on the critical buckling load.

Highly refractory peridotites in Songshugou, Qinling orogen: Insights into partial melting and melt/fluid-rock reactions in forearc mantle

Cao, Y.,Song, S.,Su, L.,Jung, H.,Niu, Y. Universitetsforlaget ; Elsevier Science Ltd 2016 Lithos Vol.252 No.-

<P>The Songshugou ultramafic massif is located in the eastern segment of the Qinling orogenic belt, central China. It is a large spinel peridotite body dominated by coarse-grained, porphyroclastic, and fine-grained dunite with minor harzburgite, olivine clinopyroxenite, and banded/podiform chromitite. The compositions of the bulk rock dunite and harzburgite, and the constituent olivine and spinel, together with the textures and chemical characteristics of multiphase mineral inclusions, point to the highly refractory nature of these rocks with complex histories of high-temperature boninite melt generation and boninitic melt-rock reaction, probably in a young, warm, and volatile-rich forearc lithospheric mantle setting. Additionally, a subsequent low-temperature fluid rock reaction is also recorded by TiO2-rich spinel with Ti solubility/mobility enhanced by chloride- or fluoride rich subduction-zone fluids as advocated by Rapp et al. (2010). The olivine clinopyroxenite, on the other hand, was likely crystallized from a residual boninitic melt that had reacted with harzburgitic residues. The ubiquitous occurrences of hydrous minerals, such as anthophyllite, tremolite, Cr-chlorite, and serpentine (stable at lower PT crustal conditions) in the matrix, suggest that further low-temperature fluid-rock reaction (or retrograde metamorphism) has affected the original volatile-poor peridotites either in a mature and cool subduction zone, or in a continental crust during their exhumation into the Qinling collisional orogeny at early Paleozoic era, or both. The prolonged and intense ductile/brittle deformation can decrease the mineral grain size through dynamic recrystallization and fracturing, and thus aid the fluid-rock reaction or retrograde metamorphism and mineral chemical re-equilibration processes. Therefore, the Songshugou peridotites present a good example for understanding the petrogenesis and evolution of the mantle wedge, with the emphasis on the complex partial melting and melt/fluid-rock interactions in the forearc setting. (c) 2016 Elsevier B.V. All rights reserved.</P>

Identification of Quantitative Trait Loci for Cold Response of Seedling Vigor Traits in Rice

Han, L.,Qiao, Y.,Zhang, S.,Zhang, Y.,Cao, G.,Kim, J.,Lee, K.,Koh, H. Science press ; Elsevier 2007 Journal of genetics and genomics Vol.34 No.3

The quantitative trait loci (QTLs) for the seedling vigor traits under 12<SUP>o</SUP>C cold water irrigation, such as the seedling height, the seedling fresh weight, the seedling dry weight, and their cold response index, were identified using an F<SUB>2-3</SUB> population including 200 individuals and lines derived from a cross of indica and japonica ''Milyang 23/Jileng 1'' with microsatellite markers. All seedling vigor traits exhibited a continuous distribution near normal in F3 lines; these traits were quantitative traits controlled by multiple genes. Twelve QTLs conferring the seedling vigor traits under cold water irrigation were detected on chromosomes 1, 2, 7, 8, and 12, which explained the observed phenotypic variance from 5.2% to 17.9%. Among them, qCSH2 and qCSH12 were located in RM262-RM263 on chromosome 2 and RM270-RM17 on chromosome 12, respectively, which were associated with the seedling height. qSDW12 and qCSDW1 were located in RM19-RM270 on chromosome 12 and RM129-RM9 on chromosome 1, respectively, which were correlated with the seedling dry weight and its cold response index, and the explained 16.6%, 17.9%, 15.9%, and 16.2% of the observed phenotypic variation, respectively. These QTLs alleles were derived from cold-tolerant parent Jileng 1; the gene actions of the two front genes showed their additive effect, and the two genes belind showed dominant and over dominant effects, respectively.

Comparison of AIRS and IASI Radiances Using GOES Imagers as Transfer Radiometers toward Climate Data Records

Wang, L.,Wu, X.,Li, Y.,Goldberg, M.,Sohn, S.-H.,Cao, C. AMERICAN METEOROLOGICAL SOCIETY 2010 JOURNAL OF APPLIED METEOROLOGY AND CLIMATOLOGY Vol.49 No.3