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RISS 인기검색어
- 검색키워드
- 저자 : H. T. Khuat (검색결과 3 건)
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Sorting CD4+ T Cells in Blood by Using Magnetic Nanoparticles Coated with Anti-CD4 Antibody
N. T. Khuat,V. T. A. Nguyen,T. N. Phan,L. H. Hoang,C. V. Thach,N. H. Hai,N. Chau 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.6
We used Fe3O4 magnetic nanoparticles (MNPs) which are coated with antiCD4 monoclonal antibody to bind selectively onto membranes of CD4+ T cells (hereafter antiCD4-MNPs). The antiCD4-MNPs were prepared through direct covalent interaction between the carboxyl group of the antiCD4 antibody and the amino group of amino-modified MNPs. The antiCD4-MNPs were mixed with human blood cells, followed by bursting the red blood cells with hypotonic buffer; then, the antiCD4-MNPs coated cells were separated by using a magnet. We observed the number of cells bound with magnetite clusters and particles. When fluorescence isothiocyanate labeled antiCD4- MNPs was used to observe the CD4+ T cells, the fluorescent intensity was improved by about two times compared to that when cells were labeled with the antiCD4 antibody only. This is a potential method to sort helper CD4+ T cells for observation under conventional microscopes. We used Fe3O4 magnetic nanoparticles (MNPs) which are coated with antiCD4 monoclonal antibody to bind selectively onto membranes of CD4+ T cells (hereafter antiCD4-MNPs). The antiCD4-MNPs were prepared through direct covalent interaction between the carboxyl group of the antiCD4 antibody and the amino group of amino-modified MNPs. The antiCD4-MNPs were mixed with human blood cells, followed by bursting the red blood cells with hypotonic buffer; then, the antiCD4-MNPs coated cells were separated by using a magnet. We observed the number of cells bound with magnetite clusters and particles. When fluorescence isothiocyanate labeled antiCD4- MNPs was used to observe the CD4+ T cells, the fluorescent intensity was improved by about two times compared to that when cells were labeled with the antiCD4 antibody only. This is a potential method to sort helper CD4+ T cells for observation under conventional microscopes.
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H. T. Khuat,N. H. Hai,C. V. Thach,N. Chau,T. N. Phan,V. T. Nguyen 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.5
Magnetite Fe$_3$O$_4$ nanoparticles were prepared by using a co-precipitation method, then functionalized by double layers of surfactants (core/oleic acid (OA)/sodium dodecyl sulfate (SDS)). For application as a drug carrier, the double-layer-coated magnetic nanoparticles were fully loaded with the antibiotic chloramphenicol (Cm) to investigate the effect of the drug release process on the bacteria {\it Escherichia coli} (E. coli). The water-soluble Cm and Cm-coated magnetic nanoparticles (Cm-NPs) (equivalent to 50 $\mu$l of 5 -- 200 $\mu$g/ml Cm) were poured into small holes on agar plates spread with E. coli, after which the plates were incubated overnight. The diameters of the non-bacteria circles were shown to gain their maximal values after 14 h, then to gradually decrease in almost all samples. Nevertheless, the circles created by Cm-NPs were about 1.5 times larger than those for the control Cm. The speed of bacterial lawn re-grown in the case of the Cm-NPs was obviously slower than that of the controlled Cm. We conclude that the magnetic nanoparticles gradually release the antibiotic, thereby maintaining the stability and the effect of the antibiotic longer than that of the conventional water-soluble antibiotic.
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