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Synthetic biology for advanced bioprocess: novel enzymes, pathway, and cell-cell communications
Gyoo Yeol JUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Pathway optimization of microbial metabolism is essential for the production of commercially valuable chemicals such as biofuels, platform chemicals and biologically active compounds. To achieve the successful design or redesign of microbial metabolism, a variety of synthetic biology tools should be required. In this study, we developed the general tools such as UTR Designer and Riboselector to modify the microbial physiology for the production of value-added chemicals at the various levels including translation and protein levels. In addition, we recently isolated a novel microorganism that can utilize macroalgae with fast metabolic activity. To engineer this organism, synthetic biology tools were developed based on the information obtained from systems biology approach. In addition, synthetic cell-cell communications were also developed to dynamically optimize their population in response to the concentration of product. Conclusively, the potentials of the platform technology developed in this study for the application to the production of biofuels and a variety of value-added chemicals.
Engineering of Glyoxylate Cycle for Efficient Tyrosine Production from Acetate in Escherichia coli
Gyoo Yeol JUNG,Minji JO,Myung Hyun NOH,Hyun Gyu LIM,Chae Won KANG,Kumyoung JEUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Acetate is one of promising feedstocks owing to its cheap price and great abundance. In this study, we engineered a previously reported strain, SCK1, for efficient production of tyrosine from acetate. Initially, the acetate uptake and gluconeogenic pathway were amplified to maximize the flux toward tyrosine. As flux distribution between glyoxylate and TCA cycles is critical for efficient precursor supplementation, the activity of the glyoxylate cycle was precisely controlled by expression of isocitrate lyase gene under different-strength promoters. Consequently, the engineered strain with optimal flux distribution produced 0.70 g/L tyrosine with 20% of the theoretical maximum yield which are 1.6-fold and 1.8-fold increased values of the parental strain. This is the first demonstration of tyrosine production from acetate. Our strategies would be widely applicable to the production of various chemicals from acetate in future.