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( Seong Hee Park ),( Sung Soo Jun ),( Gyn Heung An ),( Young Nam Hong ),( Min Chul Park ) 한국식물학회 2003 Journal of Plant Biology Vol.46 No.4
Trehalose and LEA proteins, representative low MW chemicals that are synthesized under dehydration, are known to protect plants from drought stress. To compare their effectiveness on enhancing tolerance against various abiotic stresses, we generated transgenic Chinese cabbage plants overexpressing E. Coli trehalose-6-phasphate synthase gene (otsA) or hot pepper (Capsicum annuum) LEA protein gene (CaLEA), Both transgenic plants exhibited altered phenotype including stunted growth and aberrant root development. When subjected to drought, salt or heat stress, these plants showed remarkably improved tolerance against those stresses compared with nontransformants. After dehydration treatment, leaf turgidity and fresh weight was better maintained in both transgenic plants. CaLEA-plants performed somewhat better under dehydrated condition. When treated with 250 mM NaCl, both otsA-plants and CaLEA-plants remained equally healthier than nontransformants in maintaining leaf turgidity and delaying necrosis. Furthermore, leaf Chl content and Fv/Fm was maintained considerably higher in both transgenic plants than nontransformants. After heat-treatment at 45℃, both transgenic plants appeared much less damaged in external shape and PSⅡ function, but LEA proteins were more protective. Our results indicate that although both trehalose and LEA proteins are effective in protecting plants against various abiotic stresses, LEA proteins seem to be more promising in generating stress-tolerant transgenic plants.
Differential Expression of the Potato Proteinase Inhibitor Ⅱ Promoter in Transgenic Tobacco
Kim, Su Jin,Lee, Mi-Yeon,An, Gyn Heung,Kim, Seung-Ryong 한국식물학회 2000 Journal of Plant Biology Vol.43 No.1
We studied temporal and spatial expression patterns of the potato proteinase inhibitor Ⅱ (PI-Ⅱ) promoter, using trans-genie tobacco (Nicotiana tabacum L cv. Xanthi) plants that carried a fusion between the PI-Ⅱ promoter and the chloramphenicol acetyltransferase (cat) gene. PI-Ⅱ promoter activity was low when plants were young, but increased as plants grew. In 8-week-old plants, old leaves showed higher activity than young leaves. At flowering stage (ca. 15 weeks), the overall promoter activity was reduced to a lower level except in the petals. Compared with stems or petioles at the flowering stage, the roots and floral organs showed minimal activity for the PI-Ⅱ promoter. We used several environmental stimuli to examine the induction of the PI-Ⅱ promoter in different organs. Promoter induction was effected by wounding or methyl jasmonate in stems, petioles, sepals, and leaves. The induction was highest in leaves, as was sucrose-enhanced wound induction. These results suggest that the PI-Ⅱ gene is temporally and spatially regulated. We also established a transient assay system in tobacco BY2 suspension cells to elucidate the upstream regulatory region of the PI-Ⅱ promoter. A field strength of 0.75㎸/㎝ and 400㎌ capacitance were optimal electroporation conditions for our transient assay.
Comparison and Evaluation on the Chemical Constituents of Progeny in T-DNA Inserted Rice
Qin, Yang,Kim, Suk-Man,An, Gyn-Heung,Sohn, Jae-Keun The Korean Society of Crop Science 2008 한국작물학회지 Vol.53 No.2
With the development of diverse agricultures worldwide, biofortified rice noted for its preferable marketability and palatability plays an important role in the world's agricultural economics and rice breeding programs. In this report, several $M_5$ of T-DNA inserted lines derived from the donor cultivars, 'Hwayong' and 'Dongjin', were selected for high or low protein, high lipid and low amylose content, respectively. The coefficients and ranges of variation for the chemical constituents between $M_4$ and $M_5$ T-DNA inserted lines were evaluated in comparison with those of the donor varieties. Results indicated that T-DNA insertion might be an effective way to generate useful variations for chemical composition of rice grains which could be used for the development of biofortified rice cultivars.