흰쥐 해마박편에서 veratrine과 고농도 포타슘자극시 칼슘이온이 gamma-aminobutyric acid 유리에 미치는 영향에 관한 연구 : A role of calcium

강수만,김형룡,김관식,정동균 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2

Present study was performed to clarify the effect of calcium on the release of gamma-aminobutyric acid (GABA) employing hippocampal slices. Hippocampal slices(300-400㎛ thick) were prepared by the method of Kim et al(1988) and pre-equilibrated in Krebs-bicarbonate medium(KBM, pH 7.4) for 1hr at 37℃. In case of veratrine-induced GABA release, pre-equilibrated slices were incubated in fresh KBM and then veratrine (25μM)-containing KBM for 10min period in the presence or absence of 2.5mM Ca^2+. In case of potassium-induced GABA relaese, pre-equilibrated slices were incubated in fresh KBM and then potassium(50mM)-containing KBM for 5min period in the presence or absence of 2.5mM Ca^2+. Basal and veratrine and potassium-induced release of GABA was determined from recovered medium by HPLC. The observed results were as follows: 1. The release of GABA induced by the 10min-exposure of 25μM veratrine and 5min-exposure of 50mM potassium in the presence of 2.5mM Ca^2+ was 228.9±11.2 nmol and 100.1±8.9nmol, respectively. When compared with released amounts of GABA during the corresponding spontaneous periods, these were 6.8 and 4.6-fold increase respectively. 2. The release of GABA induced by the 10min-exposure of 25μM veratrine and 5min-exposure of 50mM potassium in the absence of Ca^2+ was 381.4±30.2 nmol and 55.1±4.1 nmol, respectively. When compared with released amounts of GABA during the corresponding spontaneous periods, these were 11.3 and 2.4-fold increase respectively.

흰쥐 해마박편에서 acetylcholine이 gamma-aminobutyric acid 유리에 미치는 영향에 관한 연구

김익현,김형룡,김관식,정동균 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2

Present study was performed to clarify the effect of acetylcholine on the release of gamma-aminobutyric acid(GABA) employing hippocampal slices. Hippocampal slices (300∼400㎛ thick) were prepared by the method of Kim et al(1988) and pre-equilibrated in Krebs-bicarbonate medium(KBM, pH 7.4) for 1hr at 37℃. Pre-equilibrated slices were incubated in fresh KBM and then potassium(50mM)-containing KBM for 5 min period. Basal and potassium-induced release of GABA were determined from recovered medium by HPLC. After 30min resting period, in the presence of physostigmine(20μM) slices were reincubated in acetylcholine-containing KBM and acetylcholine plus potassium-containing medium consecutively for 5min period each to investigate the effect of acetylcholine on basal or potassium-induced GABA release from hippocampal slices. The observed results were as follows: 1. The release of GABA induced by the first and second 5 min-exposure of 50mM potassium was 107.3±8.2 nmol and 90.6±3.2nmol, respectively. When compared with released amounts of GABA during the corresponding spontaneous periods, these were 4.6 and 4.6-fold increase respectively. 2. Physostigmine(20μM) had no significant effect on the spontaneous release of GABA. 3. Acetylcholine(10-1000μM) increased spontaneous and potassium-induced GABA release in a dose-dependent manner.

Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

Kim, Hyung-Keun,Song, Mina,Jun, ji-Hae,Woo, Kyung-Mi,Kim, Gwan-Shik,Baek, Jeong-Hwa The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone merabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and 1,25(OH)_(2)vitaminD_(3)(VD3) by using mouse oligo 11 K gene chip. In the presence of 10 mM[Ca^(2+)]e or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expressions of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high ectracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated;slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated;s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracelluar calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

일측성 질폐쇄 및 동측 신장 무발생을 동반한 중복자궁 2예

윤일영,여소진,김형문,김정식,김태희,이해혁,남계현,이권해,김준모,김민의 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Uterus didelphys with unilateral obstructed hemivagina and ipsilateral renal agenesis usually presents after menarch with progressive abdominal pain during menses secondary to hematocolpos, and indeed a very rare congenital anomaly due to Mullerian duct malformation. An accurate and prompt diagnosis is of importance to permit treatment and to assure the future fertility of the patient. Pelvic ultrasound, physical examination and pelvic MRI established a diagnosis of hematometracolpos secondary to uterus didelphys with unilateral imperforate hemivagina. An incision in the vaginal septum allowed drainage of the hematocolpos, providing relief of the patients symptoms. We report two case of uterus didelphys with obstructed hemivagina with brief review of the literature.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

임베디드 장치용 사용자 인터페이스 라이브러리 설계 및 개발

김시관,홍상렬,박형순 금오공과대학교 2006 論文集 Vol.27 No.-

임베디드 시스템 장치에서 사용자에게 정보를 제공하고 사용자의 입력을 받기 위한 언터페이스의 중요성은 인터넷의 발전 및 휴대 전화 PDA 등과 같은 개인 정보 접속 장치의 대중화로 점차 커져가고 있다. 개인 정보 접속 장치뿐만 아니라 산업 현장에서 사용되고 있는 임베디드 시스템에서도 장치 제어 및 감시에 사용될 GUI의 요구 증가는 계속 되고 있다. 하지만 임베디드 장치의 제어 및 감시를 위한 GUI 라이브러리의 개발은 아직 미비한 실정이다. 따라서 임베디드 시스템 장치를 제어하고 감시하기 위한 기존의 GUI 라이브러리 보다 더 발전된 Gill 라이브러리를 설계하고 개발하였다. 그리고 새로운 GUI 리아브러리를 이용한 데모 프로그램을 작성하였다.

LCAO기저함수와 슈도포텐셜을 이용한 Sn의 에너지밴드 계산

김형관 漢陽大學校 自然科學硏究所 1996 自然科學論文集 Vol.15 No.-

슈도포텐셜을 이용한 LCAO법으로 금속성 반도체인 Sn 결정의 에너지 밴드를 계산하였다. Hartree-Fock-Slater의 방법으로 원자의 파동함수를 계산한 후 1s∼5d 궤도를 이용하여 Bloch 기저함수를 구성하였다. LCAO법에서 나타나는 다중적분의 계산을 위하여는 이산변분(discrete variational)법을 사용하였고 2152점의 대표점을 선택하였다. 본 계산의 결과는 평면파를 이용한 EPM의 결과와 잘 일치하여 LCAO법에 의한 에너지 밴드 계산도 정확한 결과를 얻을 수 있다는 결론을 얻게 되었다 The energy bands of metallic semiconductor Sn are calculated by the method of LCAO using the pseudopotential. The Bloch bases are constructed by 1s∼5d atomic orbitals calculated by the Hartree-Fock-Slater method. We use the discrete variational method for the calculation of the inevitable multi-center integral in the method of LCAO, and we select 2152 sample points for the calculation. We obtain very good agreement with the plane wave EPM results and conclude that the energy band calculation by the method of LCAO can produce the exact results.

Lidocaine이 흰쥐 해마박편에서 Gamma-Aminobutyric Acid 유리에 미치는 영향에 관한 연구

김형룡,김관식,정동균 대한구강생물학회 1988 International Journal of Oral Biology Vol.12 No.2

Present study was conducted to clarify the role of GABA in lidocaine-induced convulsion employing hippocampal slice. Hippocampal slices(300-400㎛) were prepared from hippocampal tissue blocks using a glass guide while the tissue was continuously moistened with oxygenated (95% O_2-5% CO_2) Krebs-bicarbonate media (KB) at 37℃. After preparation, the slices were equilibrated for 1hr before biochemical testing in KB. Individual equilibrated slices were transferred to tissue holder, incubated for 5min in fresh oxygenated KB, and then exposed for 5min to 50mM K^+ KB and transferred again to fresh KB for 30min rest period. Each slice served as its own control by exposing it a second time to 50mM K^+ KB for 5min after the 30min rest and a second 5min incubation in fresh KB. After the 30min rest and a second 5min incubation in fresh KB, slices were transferred again to fresh KB containing 1, 2, 3, or 4mM lidocaine, incubated for 10min and then exposed to 50mM K^+ KB+lidocaine solution for 5min. The results were as follows : 1. The release of GABA induced by the first and second 5min exposure of 50mM K^+ was 95.9±17.28nmol and 80.6±13.04nmol, respectively. When compared with released amounts of GABA during the corresponding spontaneous periods, these were 4.7 and 4.9-fold increase respectively and revealed that consecutive K^+─exposure increased GABA release with similar fashion. 2. The concentration of lidocaine necessary for 50 percent inhibition(IC_50) of potassium─induced release of GABA was 1.82mM.

Nitric Oxide가 골개조에 미치는 영향

방병관,강정숙,채한정,김형룡 원광대학교 생체재료·매식연구소 1998 원광생체재료·매식 Vol.7 No.1

Nitric oxide(NO) is known to be implicated in bone metabolism, especially as a mediator of cytokine effects for remodeling of bone tissue. In this study we examine whether NO affects osteoblast activation of osteoblast or osteoclastic differentiation in primary mouse osteblast-like and osteosarcoma ROS 17/2.8 cells. Primary osteoblast and ROS 17/2.8 cells release NO upon stimulation of interleukin-1β, tumour necrosis factor-α, and interferon-γ. Sodium nitroprusside, a donor of NO, increases the activity of alkaline phosphatase in ROS 17/2.8 cells as well as the number of calcified nodule formations in primary mouse osteoblast-like cells. Sodium nitroprusside also completely also completely inhibites 1 α,25-(OH)_2D_3-induced osteoclast genration in a high concentration (100 μM). However, low concentrations of sodium nitroprusside (3∼30 μM) significantly increase the generation of osteoclasts. These results that NO appears to be an important regulatory molecule in the processes of bone formation and resorption. Hence, NO may be involved in the pathogenesis of bone loss in diseases associated with cytokine activation such as periodontal disease and rheumatoid arthritis.

Propranolol이 흰쥐 해마박편에서 GABA유리 및 CA1 추체세포의 활성에 미치는 영향

박정희,김형룡,김관식,정동균 대한구강생물학회 1992 International Journal of Oral Biology Vol.16 No.2

Present study was performed to clarify the effect of propranolol on the GABA release and CAl hippocampal synaptic activity employing rat hippocampal slices which appear to contain most of the intrinsic and afferent circuits and to maintain the physiological characteristics seen in the hippocampus in vivo. Hippocampal slices were prepared and pre-equilibrated in Krebs-bicarbonate medium(KBM, pH 7.4) for 1 hr at 37℃. Pre-equilibrated slices were incubated in fresh KBM and then veratrine-containing KBM for 10min period. Basal and veratrine-induced GABA release were determined from recovered medium by HPLC. After 30min resting period, slices were reincubated in propranolol-containing KBM and propranolol plus veratrine-containing medium consecutively for 10min period each to investigate the effect of propranolol on basal or veratrine-induced GABA release from hippocampal slices. Extracellular population spike from CAl stratum pyramidale was measured with tungsten electrode having a tip resistance of 3-5㏁. Propranolol inhibited veratrine-induced GABA release in a dose-dependent manner. Propranolol reduced orthodromically evoked population field potentials from CAl stratum pyramidale in a concentration-dependent manner. The inhibitory effect of propranolol on GABA release and evoked population field potentials seems to be a result of sodium channel blockade.