TSPAN12 Precedes Tumor Proliferation by Cell Cycle Control in Ovarian Cancer

Guohua Ji,Hongbin Liang,Falin Wang,Nan Wang,Songbin Fu,Xiaobo Cui 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.7

TSPAN12, a member of the tetraspanin family, has been highly connected with the pathogenesis of cancer. Its biological function, however, especially in ovarian cancer (OC), has not been well elucidated. In this study, The Cancer Genome Atlas (TCGA) dataset analysis revealed that upregulation of TSPAN12 gene expression was significantly correlated with patient survival, suggesting that TSPAN12 might be a potential prognostic marker for OC. Further exploration showed that TSPAN12 overexpression accelerated proliferation and colony formation of OVCAR3 and SKOV3 OC cells. Knockdown of TSPAN12 expression in A2780 and SKOV3 cells decreased both proliferation and colony formation. Western blot analysis showed that several cyclins and cyclin-dependent kinases (CDK) (e.g., Cyclin A2, Cyclin D1, Cyclin E2, CDK2, and CDK4) were significantly involved in the regulation of cell cycle downstream of TSPAN12. Moreover, TSPAN12 accelerated mitotic progression by controlling cell cycle. Thus, our data demonstrated that TSPAN12 could be a novel molecular target for the treatment of OC.

TSPAN12 Precedes Tumor Proliferation by Cell Cycle Control in Ovarian Cancer

Ji, Guohua,Liang, Hongbin,Wang, Falin,Wang, Nan,Fu, Songbin,Cui, Xiaobo Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.7

TSPAN12, a member of the tetraspanin family, has been highly connected with the pathogenesis of cancer. Its biological function, however, especially in ovarian cancer (OC), has not been well elucidated. In this study, The Cancer Genome Atlas (TCGA) dataset analysis revealed that upregulation of TSPAN12 gene expression was significantly correlated with patient survival, suggesting that TSPAN12 might be a potential prognostic marker for OC. Further exploration showed that TSPAN12 overexpression accelerated proliferation and colony formation of OVCAR3 and SKOV3 OC cells. Knockdown of TSPAN12 expression in A2780 and SKOV3 cells decreased both proliferation and colony formation. Western blot analysis showed that several cyclins and cyclin-dependent kinases (CDK) (e.g., Cyclin A2, Cyclin D1, Cyclin E2, CDK2, and CDK4) were significantly involved in the regulation of cell cycle downstream of TSPAN12. Moreover, TSPAN12 accelerated mitotic progression by controlling cell cycle. Thus, our data demonstrated that TSPAN12 could be a novel molecular target for the treatment of OC.

Enhance Transparency of Force Feedback Interaction Series Mechanism by SMC Strategy

Zhi Hu,Yueying Wang,Guohua Cui,Dan Zhang 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.7

To enhance the fidelity of virtual surgery, the force feedback interaction mechanism would simulatethe force information in real surgery. This paper first introduces evaluation methods of fidelity, then it exploresevaluation methods of force feedback fidelity, and put up the evaluation methods and indexes of force feedbackfidelity. In rigid force feedback interaction mechanism, fidelity is mainly shown as transparency. It means thetransfer function of the mechanism needs approach to 1, while friction, gravity, slip of the linear drive and otherfactors would affect transparency of the mechanism. This study introduces a 5-DOF (degree of freedom) forcefeedback series mechanism with long stroke. It discusses gravity compensation and simulation of typical virtualenvironment. It uses sliding mode control (SMC) to eliminate the effect of parameter variety, inaccurate modelingand other factors to improve system robustness. And it adds disturbance observer to the controller to eliminategravity inaccuracy. Finally, it evaluates the enhancement of force feedback fidelity.

Investigate the Effect of Potassium on Nodule Symbiosis and Uncover an HAK/KUP/KT Member, GmHAK5, Strongly Responsive to Root Nodulation in Soybean

Liu Jianjian,Liu Jinhui,Cui Miaomiao,Chen Xiao,Liu Junli,Chen Jiadong,Chen Aiqun,Xu Guohua 한국식물학회 2022 Journal of Plant Biology Vol.65 No.6

Leguminous plants form root nodule organs with soil rhizobia bacteria, which can fix atmospheric nitrogen (N2) and supply ammonia to the host plant. It is established that the symbiotic N fixation efficiency is substantially influenced by plant nutrients, such as molybdenum (Mo), phosphorus (P), copper (Cu), and zinc (Zn). Potassium (K+) is the most abundant cation in plant cells; however, little evidence regarding the potential link between K nutrition and symbiotic N fixation efficiency is available to date. Here, we showed that K+ deficiency reduced the efficiency of symbiotic nitrogen fixation, and inoculated with rhizobia strain USDA110 could improve plant K+ acquisition. Furthermore, we identified a potassium transporter gene (GmHAK5) that was highly expressed in the root steles and in the vasculature cells of nodules. The GmHAK5 protein was localized at the plasma membrane and could rescue the growth phenotype of yeast K+ uptake-defective mutant. The results obtained from this study provides new insights for the understanding of the potential role of K+ nutrition in modulating symbiotic N fixation in soybean.

Identification of Novel Subregions of LOH in Gastric Cancer and Analysis of the HIC1 and TOB1 Tumor Suppressor Genes in These Subregions

Jingcui Yu,Songbin Fu,Peng Liu,Xiaobo Cui,Yu Sui,Guohua Ji,Rongwei Guan,Donglin Sun,Wei Ji,Fangli Liu,An Liu,Yuzhen Zhao,Yang Yu,Yan Jin,Jing Bai,Jingshu Geng,Yingwei Xue,Jiping Qi,Ki-Young Lee 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.1

Previously, we identified 3 overlapping regions showing loss of heterozygosity (LOH, R_1-R_3 from 11 to 30 cM) on chromosome 17 in 45 primary gastric cancers (GCs). The data indicated the presence of tumor suppressor genes (TSGs) on chromosome 17 involved in GC. Among the putative TSGs in these regions, HIC1 (in SR_1) and TOB1 (in SR_3) remain to be examined in GC. By immunohistochemistry (IHC), methylation-specific PCR (MSP) and western blot, we evaluated the expression and regulation status for HIC1 and TOB1 protein in GC. We narrowed down the deletion intervals on chromosome 17 and defined five smaller LOH subregions, SR_1-SR_5 (0.54 to 3.42 cM), in GC. We found that HIC1 had downregulated expression in 86% (91/106) and was methylated in 87% (26/30) of primary GCs. Of the primary GCs showing downregulation of HIC1 protein, 75% (18/24) had methylated HIC1 gene. TOB1 was either absent or expressed at reduced levels in 75% (73/97) of the GC samples. In addition, a general reduction was found in total and the ratio of unphosphorylated to phosphorylated TOB1 protein levels in the differentiated GC cell lines. Further analysis revealed significant simultaneous downregulation of both HIC1 and TOB1 protein in GC tissue microarray samples (67%, 52/78) and in primary GCs (65%, 11/17). These results indicate that silencing of HIC1 and TOB1 expression is a common occurrence in GC and may contribute to the development and progression of the disease.

Preparation of Nano-Convex and Nano-Concave-Patterned Polyimide Surfaces and their Nano-Tribological Behavior

Chunxia Wu,XIAOLIANG ZHANG,HONGWEI CHE,JINGBO MU,Guangshuo Wang,Zhixiao Zhang,Guohua Cui 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.4

In this work, nano-convex-patterned polyimide surface (notated as 1-sample) and nano-concavepatterned polyimide surface (notated as 2-sample) were prepared by self-assembly and etching. Atomic force microscope (AFM) with a colloidal probe was used to examine the adhesion and nano-tribological behavior of the 1-sample and 2-sample. Results suggest that the 1-sample and 2-sample can decrease the surface friction and adhesive forces because of the decreased contact area between the contacting pairs. The friction forces of the 1-sample and 2-sample increased with the increase in sliding velocity and applied load. Moreover, the nano-concave pattern is more effective in reducing the adhesive force than the nano-convex pattern because of its higher surface roughness. However, the nano-convex patterning is more effective in reducing the friction force than the nano-concave patterning because of the smaller area of contact between the 2-sample and the colloidal probe.