http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Guochao Zheng,Wei Hu,Yuanjia Liu,Qin Luo,Liping Tan,Guoqing Li 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.
Shengjia Huang,Xinya Liu,Bo Xiong,Xia Qiu,Guochao Sun,Xiaojia Wang,Xu Zhang,Zhixiang Dong,Zhihui Wang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3
The nomilin and limonin content in citrus fruitsof different varieties was determined at fruit growth andmaturation stages by HPLC. The results showed that thetwo limonoids can be separated, identified, and quantifiedin citrus fruits within 10 min by the developed method. Themethod exhibited good precision, repeatability, stability,and recovery rate. The content of limonin and nomilin inmost citrus fruits presented an increasing trend initially,and then decreased during fruit growth and maturation; apeak was observed at the young fruit or fruit expansionstage. The dropped fruits also contained some amount oflimonoids, suggesting their industrial application. Thevariation and cluster analyses results revealed that theorange varieties contained the highest amount of limonoidsat the mature stage. The results of this study will enablebetter use of citrus limonoids.
Yali Ma,Jiayong Wei,Cancan Li,Chen Liang,Guochao Liu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.7
Aiming at the problem that the multi-objective evaluation of performance and the weighting coefficients of indexes is difficult to find, a fuzzy comprehensive performance evaluation method of rolling linear guide (RLG) is proposed. Considering the static and dynamic indexes, a structure model containing nine weighting coefficients was constructed. The performance evaluation function was established by the compromise programming method and the average power method, while weighting coefficients were determined by the improved analytic hierarchy process (I-AHP); the mathematical model was obtained to evaluate the RLG in a certain application. The evaluation results show that the confidence of the static, dynamic and comprehensive performance of the RLG is 59.21 %, 81.97 %, and 63.34 %, respectively. The feasibility of the method was verified by numerical simulation. This method indicates the pros and cons of the performance of the RLG and also indicates the direction for its optimized design.
Guo, Chao,Liu, Ye,Yu, Haoran,Du, Kun,Gan, Yiru,Huang, He The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7
The Ω-loop is a nonregular and flexible structure that plays an important role in molecular recognition, protein folding, and thermostability. In the present study, molecular dynamics simulation was carried out to assess the molecular stability and flexibility profile of the porcine trypsin structures. Two Ω-Loops (fragment 57-67 and fragment 78-91) were confirmed to represent the flexible region. Subsequently, glycosylation site-directed mutations (A73S, N84S, and R104S) were introduced within the Ω-loop region and its wing chain based on its potential N-glycosylation sites (Asn-Xaa-Ser/Thr consensus sequences) and structure information to improve the thermostability of trypsin. The result demonstrated that the half-life of the N84S mutant at 50℃ increased by 177.89 min when compared with that of the wild-type enzyme. Furthermore, the significant increase in the thermal stability of the N84S mutant has also been proven by an increase in the T<sub>m</sub> values determined by circular dichroism. Additionally, the optimum temperatures of the wild-type enzyme and the N84S mutant were 75℃ and 80℃, respectively. In conclusion, we obtained the thermostability-improved enzyme N84S mutant, and the strategy used to design this mutant based on its structural information and N-linked glycosylation modification could be applied to engineer other enzymes to meet the needs of the biotechnological industry.