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Shunfeng Li,Anjian Wang,Lina Liu,Guangrui Tian,Fangfang Xu 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.3
To improve the efficiency of polysaccharideextraction, vacuum technology was used to breakdown thecell wall to extract polysaccharides from Lentinus edodesstipe, and the optimal parameters were optimized byresponse surface methodology. Then the antioxidantactivities in vitro of the polysaccharides were evaluated. Results showed that the optimal conditions for polysaccharideextraction were temperature of 62 C, vacuum of0.08 MPa, stirring speed of 1200 r/min, time of 25 min andmaterial/liquid ratio of 1:26 (g/mL). Under these conditions,the yield of polysaccharide was 4.28%. The decolorizationrate and deproteinization rate of polysaccharidestreated by kaolin and hydrogen peroxide were higher thanthat of activated carbon. However, there was no significantinfluence on the polysaccharide retention rate, that wasabout 80%. Results also indicated that polysaccharidetreated with activated carbon could obtain higher antioxidantactivity.