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- 저자 : Gregg A. Howe (검색결과 2 건)
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Genetic analysis of the tomato inquieta mutant links the ARP2/3 complex to trichome development
정나래,김희진,황인택,Gregg A. Howe,강진호 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6
Trichomes are hair-like structures on the aerialsurface of many plant species. Trichomes are well characterizedfor their role as physical barriers and chemical defenseagainst herbivore attack. Here, we describe the characterizationof a monogenic recessive mutant of tomato (Solanumlycopersicum) called inquieta (ini). All trichome types on iniplants showed distinct morphological defects (e.g., swelling)that are known to be associated with defects in the actincytoskeleton. Genetic mapping experiments positioned theIni locus within a 1.5 cM interval on chromosome 11 thatcontains the tomato homolog of the Arabidopsis ARPC2Agene, which encodes a protein involved in nucleating thepolymerization of actin filaments. Use of ARPC2A as amolecular marker showed that this gene strictly co-segregateswith the target locus in a mapping population of 135 F2plants. Reverse transcriptase (RT)-PCR and genomic PCRexperiments showed that full-length ARPC2A is amplified inwild-type but not in the ini mutant. Flanking PCR andSouthern blot analysis showed that the ini mutation correspondsto a complex ~6-kb insertion in the 5th intron of ARPC2A. These results provide molecular evidence that alteredtrichome development in the ini mutant is caused by a defectin actin cytoskeleton formation.
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Kang, Jin-Ho,Campos, Marcelo L.,Zemelis-Durfee, Starla,Al-Haddad, Jameel M.,Jones, A. Daniel,Telewski, Frank W.,Brandizzi, Federica,Howe, Gregg A. Oxford University Press 2016 Journal of experimental botany Vol.67 No.18
<P>Trichomes are epidermal structures that provide a first line of defense against arthropod herbivores. The recessive <I>hairless</I> (<I>hl</I>) mutation in tomato (<I>Solanum lycopersicum</I> L.) causes severe distortion of trichomes on all aerial tissues, impairs the accumulation of sesquiterpene and polyphenolic compounds in glandular trichomes, and compromises resistance to the specialist herbivore <I>Manduca sexta</I>. Here, we demonstrate that the tomato <I>Hl</I> gene encodes a subunit (SRA1) of the highly conserved WAVE regulatory complex that controls nucleation of actin filaments in a wide range of eukaryotic cells. The tomato <I>SRA1</I> gene spans a 42-kb region containing both <I>Solyc11g013280</I> and <I>Solyc11g013290</I>. The <I>hl</I> mutation corresponds to a complex 3-kb deletion that removes the last exon of the gene. Expression of a wild-type <I>SRA1</I> cDNA in the <I>hl</I> mutant background restored normal trichome development, accumulation of glandular trichome-derived metabolites, and resistance to insect herbivory. These findings establish a role for <I>SRA1</I> in the development of tomato trichomes and also implicate the actin-cytoskeleton network in cytosolic control of specialized metabolism for plant defense. We also show that the brittleness of <I>hl</I> mutant stems is associated with altered mechanical and cell morphological properties of stem tissue, and demonstrate that this defect is directly linked to the mutation in <I>SRA1</I>.</P>
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