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- 저자 : Gong-yin Ye (검색결과 3 건)
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Characterization of three serotonin receptors from the small white butterfly, <i>Pieris rapae</i>
Qi, Yi-xiang,Jin, Miao,Ni, Xu-yang,Ye, Gong-yin,Lee, Youngseok,Huang, Jia Elsevier 2017 Insect biochemistry and molecular biology Vol.87 No.-
<P><B>Abstract</B></P> <P>Serotonin (5-hydroxytryptamine, 5-HT) plays a key role in modulating diverse physiological processes and behaviors in both protostomes and deuterostomes. These functions are mediated through the binding of serotonin to its receptors, which are recognized as potential insecticide targets. We investigated the sequence, pharmacology and tissue distribution of three 5-HT receptors (Piera5-HT<SUB>1A</SUB>, Piera5-HT<SUB>1B</SUB>, Piera5-HT<SUB>7</SUB>) from the small white butterfly <I>Pieris rapae</I>, an important pest of cultivated cabbages and other mustard family crops. Activation of Piera5-HT<SUB>1A</SUB> or Piera5-HT<SUB>1B</SUB> by 5-HT inhibited the production of cAMP in a dose-dependent manner. Stimulation of Piera5-HT<SUB>7</SUB> with 5-HT increased cAMP level significantly. Surprisingly, with the exception of 5-methoxytryptamine, agonists including α-methylserotonin, 8-Hydroxy-DPAT and 5-carboxamidotryptamine activated these receptors poorly. The results are consistent with previous findings in <I>Manduca sexta</I>. All three receptors were blocked by methiothepin, but ketanserin and yohimbine were not effective. The selective mammalian 5-HT receptor antagonists SB 216641 and SB 269970 displayed potent inhibition effects on Piera5-HT<SUB>1B</SUB> and Piera5-HT<SUB>7</SUB> respectively. The results we achieved here indicate that the pharmacological properties of Lepidoptera 5-HT receptors are quite different from those in other insects and vertebrates and may contribute to development of new selective pesticides. This study offers important information on three 5-HT receptors from <I>P. rapae</I> that will facilitate further analysis of the functions of 5-HT receptors in insects.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Serotonin receptors Piera5-HT<SUB>1A</SUB>, Piera5-HT<SUB>1B</SUB>, and Piera5-HT<SUB>7</SUB> from <I>Pieris rapae</I> were cloned and examined. </LI> <LI> Upon serotonin activation, Piera5-HT<SUB>1A</SUB> and Piera5-HT<SUB>1B</SUB> inhibited cAMP production whereas Piera5-HT<SUB>7</SUB> induced it. </LI> <LI> All three receptors were poorly activated by serotoninergic agonists, indicating their distinct pharmacological properties. </LI> <LI> All three receptors were blocked by methiothepin; SB 216641 and SB 269970 inhibited Pr5-HT<SUB>1B</SUB> and Pr5-HT<SUB>7</SUB>, respectively. </LI> <LI> All three receptors were highly expressed in neuronal tissues while Pr5-HT<SUB>7</SUB> mRNA predominated in the gut. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Guo-Xing Wu,Xi Gao,Qing Tan,Zheng-Yue Li,Cui Hu,Gong-yin Ye 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.2
In order to establish a physiological link between antioxidases and the resistance level of insects to cadmium(Cd), natural populations of Boettcherisca peregrina (Diptera: Sarcophagidae)weremaintained for 20 generationsand reared either on an uncontaminated diet or on a diet contaminated with cadmium (Cd) at a concentrationequivalent to the median lethal concentration (LC50) as determined every five generations. A relatively susceptiblestrain (S) and a Cd-resistant strain (R) were selected. Themetal accumulation, growth and development, reproduction,and antioxidant enzyme activities in these strains were analyzed. The results showed that R-strain organismshad enhanced juvenile survivorship, increased Cd accumulation, and increased adult female fecundity whencompared with S-strain. The larval enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathionereductase (GR), and glutathione S-transferase (GST) in R-strain larvae were higher than those in S-strain larvaewhen fed diets with or without Cd. This indicates that Cd resistance in B. peregrina larvae is mediated by SOD,CAT, GR, and GST.
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