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고추균에서 아밀라제 생합성의 Catabolite Repression 에 관하여는 Amyl 단백질의 특성에 관한 연구
원미선 Glenm H . Chambliss,송경빈 ( Misun Won,Glenn H . Chambliss,Kyung Bin Song ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.2
The amyO (amylase operator) region in B. subtilis has 79% homology with lacO consensus sequence of E. coli, and 92% with galO consensus sequence of E. coli. The AmyI (amyO binding protein) protein was purified as an amyO-binding protein specifically involved in the catabolite repression of α-amylase synthesis. Run-off transcription experiment indicated that the AmyI protein inhibited the initiation of the amyE transcription from amylase promoter in vitro. Binding studies of the AmyI protein demonstrated positive cooperativity with amyO, wild type operator, but non-cooperativity with amyO^(gra-10). The binding of the AmyI protein to wild type amyO was less sensitive to salt concentration than that of the AmyI to amyO^(gra-10). This evidence suggests that the AmyI protein be an amylase repressor protein to regulate amylase synthesis in vivo.
The Regulation of Alpha-Amylase Synthesis in Bacillus subtilis
WON, MISUN,CHAMBLISS, GLENN H.,SONG, KYUNG BIN 한국미생물 · 생명공학회 1991 Journal of microbiology and biotechnology Vol.1 No.4
In B, subtilis, α-amylase synthesis is regulated by amyR located directly on the upstream of amyE. Three different amyR alleles have been reported, amyRl, amyR2 and amyR3. Strains bearing the gra-10 mutation which confers derepression for catabolite repression has G→A transition mutation at +5 of amyR1. S1 nuclease mapping demonstrated that transcription initiated at 8 bases downstream from the -10 region of putative Eσ^A promoter P1 in amyR1 and gra-10. In amyR2, the major transcription initiatd at the same place and the minor, 10 bases downstream from -10 of P2. The transcript from P2 contributed approximately 15-20% of total amyE mRNA. S1 nuclease protection experiment indicated that amyE mRNA levels corresponded to the rate of synthesis assumed by specific activities of α-amylase in culture supernatants, suggesting that α-amylase synthesis is regulated at the level of transcription.