딤플을 적용한 평판에 대한 항력 감소 연구

백부근(Bu-Geun Paik),편영식(Young-Sik Pyun),김준형(Jun-Hyung Kim),김경열(Kyung-Youl Kim),김기섭(Kim1 ?Ki-Sup),정철민(Chul-Min Jung),김찬기(Chan-Ki Kim) 대한조선학회 2012 大韓造船學會 論文集 Vol.49 No.4

강릉연안 홍조 긴잎돌김(Porphyra pseudolinearis Ueda)의 원형질체 분리

김형근,김영대,전방욱 강릉대학교 동해안지역연구소 2000 東海岸硏究 Vol.11 No.2

Two kinds of enzymes solution, capable of degrading the cell wall of red alga Porphyra psuedolinearis Ueda, were prepared from the abalone acetone powder and alkaline hemicellulase. Selected vegetative fronds(3cm) were washed thoroughly in sterile seawater. These blade pieces was kept overnight in an antibiotic solution, and used for isolated protoplast. The vegetative tissue was pretreated, cut into small pieces and soaked in 10% papain solution, and then the small pieces of tissue were incubated in tube containing 5ml of 10% abalone aceton powder solution and 0.01% alkaline hemicellulase solution(MES buffer and pH 6.0) respectively. Protoplasts were removed from the enzyme solution by filtration through a 40μm nylon mesh followed by centrifuge. Yields of protoplasts was higher in alkalin hemicellulase solution(6.0×106/0.1g) than in abalone acetone powder solution(5.4×105/0.1g).

철근콘크리트 보의 2단주근 부착할렬거동에 관한 실험적 연구

김혜민,이형복,강호근,윤혁기 慶南專門大學 2001 論文集 Vol.29 No.1

海上王 張保庫의 海上通路 推定에 관한 硏究

金亨根 木浦大學校 아시아文化硏究所 2000 아시아문화연구 Vol.4 No.-

In this study the writer would like to estimate the marine routes of Merchant Prince, Chang Pogo in a United Silla. For the purpose of this study the writer study the wind and current which effect the navigation of sailing ship, and ancient reference books and materials, especially the Work of Routes and Kingdoms of Ibn Khurdadhbih regarding to the ancient marine routes of Korea, China, Japan, Arab-Islam. The result of this study, in the era of three Kingdoms of the Korean Peninsular there were the Rochulsan route, the Cross route against the Yellow Sea. Tsushima route, the route of South China Sea. the route of East China Sea, the southern route of the Yellow Sea, the direct route of the East Sea and so on, and the route of the Jusan Archipelago, the route of Ryukyu. Especially in a United Silla, there were the Arab-Islam routes which connect between Korean Peninsular and Arab-Islam. Accordingly the writer estimate that Merchant Prince, Chang Pogo in an Unite Silla had traded with not only China, Japan but also Arab-Islam Countries using above marine routes.

자란,풍란 및 나도풍란의 기내 종자발아에 관한 연구

김형근,강훈,소인섭 濟州大學校 亞熱帶農業硏究所 1999 亞熱帶農業硏究 Vol.16 No.-

우리나라 자생란인 자란의 기내파종시 적절한 소독방법과 적정배지 및 순화시 생존율을 높이기 위할 목적으로 uniconazole를 처리하고, 풍란과 나도풍란의 기내파종시 발아율을 높이기 위해 천연사과쥬스를 처리한 결과는 다음과 같다. 1. 자란의 기내파종시 종자를 Wilson 용액으로 소독하여 MS 혹은 Hyponex 3g/L 기본배지에 peptone 4g/L 배지에 파종하였을 때 발아와 생장이 가장 좋았다. 2. 기내 파종후 자란의 생장을 억제하기 위하여 uniconazole을 처리한 결과 활성탄 2g/L와 uniconazole 0.01mg/L를 첨가한 처리에서 초장이 넓은 타원형으로 알맞게 억제되었고, 활성탄이 첨가되면 uniconazole 이 활성탄에 흡수되어 억제효과가 다소 적어 활성탄을 첨가하지 않은 처리는 첨가한 처리보다 심하게 억제되었다. 3. 풍란의 기내파종시 Hyponex 3g/L 배지에서는 발아 후 생장이 전혀 이루어 지지 않았고 MS배지에서는 발아만 하였으나, Hyponex 3g/L에 천연 100% 사과쥬스를 첨가한 처리에서는 pH에 관계없이 전처리에서 발아하여 NAA 1mg/L에 활성탄을 첨가한 처리에서 발아와 생장이 가장 좋았다. 4. 나도풍란의 종자 발아와 생육에 대한 배지의 적용성은 풍란의 경우와 유사한 경향을 보여주었다. pH 5.8에 활성탄을 첨가하지 않고 NAA를 1mg/L 첨가한 처리만 발아 후 생장을 하였다. 발아와 생장이 잘된 배지는 Hyponex 3g/L에 천연 사과 주스 100ml/L를 첨가한 처리였으며 PH와 활성탄 및 NAA의 첨가에 관계없이 발아와 생장이 왕성했다. In order to find proper media, accurate sterilizing method and further growth on in vitro-seeding of Bletilla striata, korean native orchid, the uniconazole were treated. And the Neofinetia falcata and Aerides japonicum were treated with natural apple juice to improve germination ratio in vitro-seeding. The results obtained were as follows : 1. Germination and growth of Bletilla striata were the highest in case of seeding to MS or Hyponex 3g/L + peptone 4g/L media after sterilized them by Wilson's solution. 2. The results to promote growth of Bletilla striata after in vitro-seeding were that leaf shape was properly restrained widely ellipse in treated with 2g/L activated charcoal and 0.01mg/L uniconazole, and the treatment of uniconazole was more restrained than the treatment of activated charcoal. 3. In vitro-seeding of Neofinetia falcata, growth after germination was not affected in Hyponex 3g/L media and a little growth is shown in MS media. But in treatment which apple juice 100 ml/L was treated with Hyponex 3g/L, sprouting is shown in every treatments regardless of pH, and the total growth of seedlings is the best in the treatment of activated charcoal 2g/L and NAA 1mg/L. 4. In case of seeding of Aerides japonicum, germination rate and growth were similar trend to Neofinetia falcata. In every treatments of MS media, germination is a little bit, but further growth after germination was shown in treatment which treated NAA 1mg/L and pH 5.8 without activated charcoal. The best germination and growth media for 2 examined seeds were the treatment which apple juice 100 ml/L was treated with Hyponex 3g/L, but there was no effect on pH level, activated charcoal and NAA.

효율적인 닫힌 빈발 시퀀스 마이닝

김형근,황환규 江原大學校 産業技術硏究所 2005 産業技術硏究 Vol.25 No.A

Recent sequential pattern mining algorithms mine all of the frequent sequences satisfying a minimum support threshold in a large database. However, when a frequent sequence becomes very long, such mining will generate an explosive number of frequent sequence, which is prohibitively expensive in time. In this paper, we proposed a novel sequential pattern algorithm using only closed frequent sequences which are small subset of very large frequent sequences. Our algorithm extends the sequence by depth-first search strategy with effective pruning. Using bitmap representation of underlying databases, we can obtain a closed frequent sequence considerably faster than the currently reported methods.

목포항의 항만현황과 개발방향에 관한 연구

김형근,신호식 木浦海洋大學校 1997 論文集 Vol.5 No.2

In this study the writer analyzed and suggested the situation of the port and development project of the Port of Mokpo which attaining 100th anniversary of opening a port. The important contents of the study can be summarized as follows: Firstly, The total length of wharf of Mokpo Port is composed of 4,964 meters. Among them, the length of cargo ships wharf is 3,962 meters and the length of passenger ships wharf is 351 meters and the length of fishing boats wharf is 633 meters. Secondly, For the last three years(1994-1996) the amount of harbour materials and the income of cargo work of the Port of Mokpo is analyzed as the lowest rank in the principal ports of the whole country. Thirdly, The development project of No.1 Wharf is suggested as a international passenger wharf and position wharf of international exchanges, and the development project of No.2,3 Wharf is suggested as a leisure wharf, and the development project of No.4,5 Wharf is suggested as a coasting passengers and tourists wharf, and the development project of Southern Port is suggested as a port of environmental fraternization, and the development project of Northern Port is suggested as a fishing port. Finally, In the municipal organization of Mokpo, the new establishment of the Department of the Ocean & Fisheries is suggested.

醮禮山 소나무林의 山火地의 二次遷移

金源,張根亨,曺英鎬,沈學輔 慶北大學校 1992 論文集 Vol.52 No.-

This report is an investigation of revestagation and secondary succession in the burned area. The forest fire occurred in Mt. Ch'olye on April, 1989. After logging of the burned pine tree, the seedlings of Pinus koraiensis were planted by the replaceable plant in there. The results are summarized as follows : Dorminant species in the first year(B-1) after fire were Miscanthus sinensis var. purpurascens, Carex humilis and Duchesnea chrysantha and those in the second year(B-2) after fire Miscanthus sinensis var. purpurascens, Carex humilis and Lespedeza cyrtobotrya. the biological spectra in both burned and unburned areas were H-D_1-R_5-e type. The indices of similarity showed that B-1 and B-2 was 0.667, B-1 and U 0.526, and B-2 and U 0.500. Degree of succession(DS) was 181, 345, 1090 and species diversity(H) 2.35, 2,73, 2.54 and evenness index(e) 0.61, 0.73, 0.73 and dominance index(C) 0.17, 0.11, 0.15, in B-1 and U site, respectively. Biomass of B-2 site was higher than that of B-1 site. Litter amount(kg/m^2) of burned and unbrurned areas were 0.017 and 0.863. Soil pH, content of total nitrogen, organic matter, organic carbon, and water holding capacity of burned area were higher than those of unburned area while content of available phosphorus, exchangeable pottassium, C/N ratio, and water content of unburned area were higher than those of burned area, respectively.

溫排水 지역 潮間帶 海藻群集의 種 多樣度

金亨根,孫徹鉉 江陵大學校東海岸地域硏究所 1993 東海岸硏究 Vol.4 No.2

古里 原子力發電所 연안에서 溫排水의 구배에 따른 潮間帶 海藻群集의 特性을 분석하기 위해 種 多樣度로서 비교하였다. 潮間帶의 潮位가 높을수록 種 豊度와 種 多樣度는 낮았으며 배수구에 가까운 곳에서 다양도의 값은 낮았다. 平均低潮線에서의 潮位(HT)와 배수구에서 떨어진 거리(DO)에 대한 種 多樣度(ASD)의 관계식은 ASD=2.1838-2.3582(HT)+0.0365(DO)로 나타났다. 배수구 주변의 海藻群集은 群集發達의 初期에서 成熟段階의 각 단계를 나타내는 것으로 간주되었다. In order to analysis the characteristics of intertidal algal communities at the coast of Kori Nuclear Power Plant located along a thermal pollution gradient were compared by the species diversity index. The higher tidal level, the lower the species richness and the diversity. The values of the diversity was also low near the outfalls. The formula of the relationship of algal species diversity(ASD) against tidal level(TL) from mean low water and the distance of the outfall(DO) is the following; ASD=2.1838-2.3582(HT)+0.0365(DO). Thermal effects near the discharge were similar to the decrease species diversity observed towards upper intertidal levels. Algal communities in the vicinity of the thermal discharge could be considered to represent the respective stages of community development from pioneer to mature.

실험 방광 종양화과정에서 Proliferating Cell Nuclear Antigen(PCNA)의 발현

김원재,송형근,이현무 충북대학교 의과대학 충북대학교 의학연구소 1992 忠北醫大學術誌 Vol.2 No.1

Fisher 344백서에 0.1% N-butyl-N-(4-hydroxybutyl) nitrosamine(BBN)을 투여하여 방광에 단순과증식(simple hyperplasia ; SH), 결절성 혹은 유두상 과증식(nodular or papillary hyperplasia ; NPH) 및 표재성 방광암(Ta, T1)을 유발시킨 후 개개의 단계의 증식성의 차이를 보고자 후기 G1 phase, S-phase 및 전기 G2/M phase를 반영하는 proliferating cell nuclear antigen(PCNA/cyclin)에 대한 anti-PCNA/cyclin monoclonal antibody를 이용하여 세포의 labeling index(LI)를 구하였다. 정상,SH,NPH,Ta 및 T1에서의 LI는 0.76±2.71,4.52±7.74,10.55±17.99,16.97±18.66, 17.04±16.10이었으며, grade I이 14.54±16.21, grade II가 19.30±16.74이었다. 방광암의 종양화 과정이 진행함에 따라 통계적으로 유의하게 LI가 증가하는 양상을 보였으며 또한 분화도가 나빠질수록 LI가 유의하게 증가하였다. 그러나 종양의 경우 즉, Ta와 T1사이에는 통계적으로 유의한 차이(P>0.4)가 관찰되지 않았다. 이러한 사실은 이들의 경우에 있어서 세포분화도상 비슷한 병기의 표재성 종양이기 때문에 LI의 차이가 없었던 것으로 생각되며 이같은 세포역동학적 분석방법에 있어서는 종양의 병기보다는 개개의 세포의 생물학적 악성도에 의해서 좌우되는 것 같다. Proliferating cell nuclear antigen(PCNA)/cyclin is a cell-cycle-regulated protein, which can be demonstrated in routinely fixed specimens. The expression of PCNA/cyclin was investigated immunohistochemically in BBN induced rat bladder tumorigenesis using anti-PCNA/cyclin monoclonal antibody in embedded tissue fixed with formalin. Sequential cellular changes(normal, simple hypernlasia, nodular or papillary hyperplasia, and transitional cell carcinoma-Ta, T1) were observed in rat urinary bladder in accordance with the duration of BBN administration. The PCNA labeling index(LI) was determined by counting the number of PCNA labeled cells in the corresponding tissue section. The average PCNA LI obtained using this method in normal, simple hyperplasia(SH), nodular or papillary hyperplasia(NPH), and bladder tumor(Ta, T1) was 0.76, 4.52, 10.55, and 17.01(16.97, 17.04), respectively. Also, higher PCNA LI was found in grade II compared to grade I. Higher PCNA LI indicated greater biological malignancy based on the fact that mean LI progressively increased with tumorigenesis. However, mean LI at Ta did not significantly differ from that of T1, which implied that Ta might reflect similar cellular characteristics as a superficial bladder tumor.