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수핵란의 전활성화가 토끼 핵이식 수정란의 체외발달에 미치는 효과
전병균,송상현,정기화,곽대오,이효종,최상용,박충생 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-
To examine the efficiency of nuclear transplantation the influence of electrical preactivation of recipient cytoplasm on the in vitro developmental potentyl in the nuclear transplant rabbit embryos were evaluated. The embryos of 16-cell stage were collected and synchronized to G₁phase of 32-cell stage. The recipient cytoplasms were obtained by removing the first polar body and chromosome mass by non-disruptive microsurgery procedure. The separated G₁phase blastomeres of 32-cell stage were out into the non-preactivated and/or the preactivated recipient cytoplasm by electrical stimulation. After culture until 20h post-hCG injection, the nuclear transplant oocytes were electrofused. The fused nuclear transplant embryos were co-cultured with rabbit oviduct for 120h, the nuclear transplant embryos developed to blastocyst stage were stained with Hoechst 33342 and their blastomere were counted. The electrofision rate was similar to the non-preactivated and preactivated recipient cytoplasm(81.8 and 85.7%, respectively). However, the in vitro developmental rate to blastocyst stage with the non-preactivated recipient cytoplasm(163.7cells), as compared with the preactivated recipient cytoplasm(85.4 cells). These results considered better that non-preactivated oocytes, MⅡ phase oocytes, were used for recipient cytoplasms in the rabbit nuclear transplant procedure.
Kim, J.H.,Lee, G.,Cho, Y.L.,Kim, C.K.,Han, S.,Lee, H.,Choi, J.S.,Choe, J.,Won, M.H.,Kwon, Y.G.,Ha, K.S.,Kim, Y.M. North-Holland ; Elsevier Science Ltd 2009 european journal of pharmacology Vol.602 No.2
We investigated the effect of desmethylanhydroicaritin (DMAI), a major compound of the Chinese herbal medicine Epimedium, on inflammatory gene expression and the NF-κB signaling pathway. We found that DMAI suppressed the expression of NF-κB-responsive genes, such as inducible nitric oxide synthase, cyclooxygenase-2, interleukin-1β, and tumor necrosis factor-α, in lipopolysaccharide (LPS)-stimulated macrophages and endotoxemic mice as well as protected mice against LPS-induced lethality. DMAI inhibited NF-κB activation through the inhibition of IκB kinase (IKK) activation, IκB phosphorylation and degradation, and NF-κB nuclear translocation in LPS-stimulated macrophages. This compound inhibited in vitro and in vivo LPS-induced phosphatidylinositol 3-kinase (PI3K) activation, phosphatase and tensin homolog deleted on chromosome 10 (PTEN) oxidation, and Akt phosphorylation, which are upstream modulators of IKK activation. Moreover, treatment with DMAI was not observed to affect the interaction between the Toll-like receptor 4, MyD88, and TRAF6 as well as mitogen-activated protein kinase activation. DMAI also suppressed intracellular H<SUB>2</SUB>O<SUB>2</SUB> accumulation, hydroxyl radical production, and glutathione oxidation without affecting superoxide generation and accumulation by NADPH oxidase. Moreover, DMAI inhibited redox-sensitive activation of the PI3K/PTEN/Akt pathway and NF-κB activation in macrophages treated with H<SUB>2</SUB>O<SUB>2</SUB>. These results indicate that DMAI negatively regulates canonical NF-κB-regulated inflammatory gene expression by functioning as an inhibitor of the NF-κB pathway through the suppression of redox-based PI3K activation and PTEN inactivation and therefore can be considered as a potential drug for inflammatory diseases.
Kook, S-H.,Son, Y-O.,Choe, Y.,Kim, J-H.,Jeon, Y-M.,Heo, J-S.,Kim, J-G.,Lee, J-C. Blackwell Publishing Ltd 2009 Journal of periodontal research Vol.44 No.3
<P>Background and Objective: </P><P>The cellular response of human gingival fibroblasts to a mechanical force is considered to be primarily anti-osteoclastic because they produce relatively high levels of osteoprotegerin. However, there is little information available on the effects of compression force on the production of osteoprotegerin and osteoclastic differentiation by these cells. In this study, we examined how mechanical force affects the nature of human gingival fibroblasts to produce osteoprotegerin and inhibit osteoclastogenesis.</P><P>Material and Methods: </P><P>Human gingival fibroblasts were exposed to mechanical force by centrifugation for 90 min at a magnitude of approximately 50 g/cm<SUP>2</SUP>. The levels of osteoprotegerin, receptor activator of nuclear factor-&kgr;B ligand (RANKL), interleukin-1&bgr; and tumor necrosis factor-&agr; were measured at various time-points after applying the force. The effect of the centrifugal force on the formation of osteoclast-like cells was also determined using a co-culture system of human gingival fibroblasts and bone marrow cells.</P><P>Results: </P><P>Centrifugal force stimulated the expression of osteoprotegerin, RANKL, interleukin-1&bgr; and tumor necrosis factor-&agr; by the cells, and produced a relatively high osteoprotegerin to RANKL ratio at the protein level. Both interleukin-1&bgr; and tumor necrosis factor-&agr; accelerated the force-induced production of osteoprotegerin, which was inhibited significantly by the addition of anti-(interleukin-1&bgr;) immunoglobulin Ig isotype; IgG (rabbit polyclonal). However, the addition of anti-(tumor necrosis factor-&agr;) immunoglobulin Ig isotype; IgG1 (mouse monoclonal) had no effect. Centrifugal force also had an inhibitory effect on osteoclast formation.</P><P>Conclusion: </P><P>Application of centrifugal force to human gingival fibroblasts accelerates osteoprotegerin production by these cells, which stimulates the potential of human gingival fibroblasts to suppress osteoclastogenesis. Overall, human gingival fibroblasts might have natural defensive mechanisms to inhibit bone resorption induced by a mechanical stress.</P>
간호대생들의 임상실습 스트레스, 학업적 자기효능감 및 진로정체감에 대한 연구
최유빈,최민지,이은형,이연우,우소연,하희주,허예지,박수현,허미래,이가현 이화여자대학교 간호과학대학 2015 이화간호학회지 Vol.- No.49
Purpose: The purpose of this study was to investigate clinical practice stress, academic self-efficacy, and career identity among undergraduate students in nursing practicum. Method: This study was a descriptive correlational design using the survey method. The study included 267 undergraduate students attending to 5 college in Seoul. The data was gathered from August 2014 to October 2014 and the data were analyzed using IBM SPSS statistics version 22.0 Program with descriptive statistics, t-test, analysis of variance with Scheffe test, and Pearson’s correlation coefficient. Results: Situational characteristics of Clinical Practice Stress(CPP) was significantly different with regards to task difficulties and efficacy self-confidence of academic self-efficacy(ASE) (r= .179, p= .003; r= .166, p= .006). Statistically significant positive relationship was founded between Personal characteristics of CPP and self-confidence of ASE (r= .239, p< .01). External regulators of CPP was significantly different according to self-confidence and task difficulties (r= .175, p= .003; r= .132, p= .028). The self-confidence and efficacy for self-confidence of ASE were a significantly correlated with Career Identity; The Career identity also was a significantly correlated with all sub-categories of CSS scale(r= -0.208, p< 0.01; r= -0.369, p< 0.01; r= -0.153, p< .011) Conclusion: This study showed positive statistical relationships associated with clinical practice stress, academic self-efficacy, and career identity as a preliminary evidence-based study for guiding education in nursing curriculum. Further research should be need for students’ future as a professional nurse.
Modification of argon impurity transport by electron cyclotron heating in KSTAR H-mode plasmas
Hong, Joohwan,Henderson, S.S.,Kim, Kimin,Seon, C.R.,Song, Inwoo,Lee, H.Y.,Jang, Juhyeok,Park, Jae Sun,Lee, S.G.,Lee, J.H.,Lee, Seung Hun,Hong, Suk-Ho,Choe, Wonho International Atomic Energy Agency 2017 Nuclear fusion Vol.57 No.3
<P>Experiments with a small amount of Ar gas injection as a trace impurity were conducted in the Korea Superconducting Tokamak Advanced Research (KSTAR) H-mode plasma (<img ALIGN='MIDDLE' ALT='${{B}_{\text{T}}}$ ' SRC='http://ej.iop.org/images/0029-5515/57/3/036028/nfaa5333ieqn001.gif'/> = 2.8 T, <img ALIGN='MIDDLE' ALT='${{I}_{\text{P}}}$ ' SRC='http://ej.iop.org/images/0029-5515/57/3/036028/nfaa5333ieqn002.gif'/> = 0.6 MA, and <img ALIGN='MIDDLE' ALT='${{P}_{\text{NBI}}}$ ' SRC='http://ej.iop.org/images/0029-5515/57/3/036028/nfaa5333ieqn003.gif'/> = 4.0 MW). 170 GHz electron cyclotron resonance heating (ECH) at 600 and 800 kW was focused along the mid-plane with a fixed major radial position of <img ALIGN='MIDDLE' ALT='$R$ ' SRC='http://ej.iop.org/images/0029-5515/57/3/036028/nfaa5333ieqn004.gif'/> = 1.66 m. The emissivity of the Ar<SUP>16+</SUP> (3.949 <img ALIGN='MIDDLE' ALT='${\mathring{\text{A}}}$ ' SRC='http://ej.iop.org/images/0029-5515/57/3/036028/nfaa5333ieqn005.gif'/>) and Ar<SUP>15+</SUP> (353.860 <img ALIGN='MIDDLE' ALT='${\mathring{\text{A}}}$ ' SRC='http://ej.iop.org/images/0029-5515/57/3/036028/nfaa5333ieqn006.gif'/>) spectral lines were measured by x-ray imaging crystal spectroscopy (XICS) and a vacuum UV (VUV) spectrometer, respectively. ECH reduces the peak Ar<SUP>15+</SUP> emission and increases the Ar<SUP>16+</SUP> emission, an effect largest with 800 kW. The ADAS-SANCO impurity transport code was used to evaluate the Ar transport coefficients. It was found that the inward convective velocity found in the plasma core without ECH was decreased with ECH, while diffusion remained approximately constant resulting in a less-peaked Ar density profile. Theoretical results from the NEO code suggest that neoclassical transport is not responsible for the change in transport, while the microstability analysis using GKW predicts a dominant ITG mode during both ECH and non-ECH plasmas.</P>
Development of Bovine Embryos Produced by Intracytoplasmic Sperm Injection (ICSI)
Ock, S.A.,Kwack, D.O.,Cho, S.R.,Cho, S.K.,Yeao, E.H.,Yoo, J.G.,Lee, Y.R.,Lee, H.J.,Choe, S.Y.,Rho, G.J. 韓國受精卵移植學會 2002 한국동물생명공학회지 Vol.17 No.1
Intracytoplasmic Sperm Injection (ICSI) has been widely used fur both human infertility and basic research. However, the high incidence of chromosomal abnormality is severe problem in cattle. Various oocyte activation stimuli, therefore, were compared by assessment of developmental capacity and chromosome analysis. Motile sperm selected by Percoll-density gradient were treated with 5 mM dithiothreitol (DTT) and injected into an oocyte matured fur 24 h. Eggs were then allocated into 5 treatment groups. Group 1 (control), sperm injection was performed without any further activation stimuli to the oocytes. Group 2 (handled control), sham injection was performed without sperm. In Group 3, oocytes exposed to 5 (M ionomycin for 5 min at 39(C. Group 4. ionomycine + 1.9 mM demethylaminopurine (DMAP, 3 h) and Group 5, ionomycine + 3 h culture in Ml99 + DMAP. Cleavage and the later development rate in Groups 1, 2 and 3 were significantly (P<0.05) lower than those in Groups 4 and 5. The incidence of chromosomal abnormality in the embryos treated directly with DMAP after ionomycine was relatively higher than in the embryo of Group 3 h, delayed DMAP treatment. From this results DMAP caused to be arrested the release of the 2nd polar body, resulting in changes of chromosomal pattern. Therefore, the time interval between ionomycin and DMAP is a crucial role in bovine ICSI.