Nucleotide sequence and genome organization of atractylodes mottle virus, a new member of the genus Carlavirus

Zhao, Fumei,Igori, Davaajargal,Lim, Seungmo,Yoo, Ran Hee,Lee, Su-Heon,Moon, Jae Sun Springer-Verlag 2015 Archives of virology Vol.160 No.11

Characteristics of a Lettuce mosaic virus Isolate Infecting Lettuce in Korea

Lim, Seungmo,Zhao, Fumei,Yoo, Ran Hee,Igori, Davaajargal,Lee, Su-Heon,Lim, Hyoun-Sub,Moon, Jae Sun The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.2

Lettuce mosaic virus (LMV) causes disease of plants in the family Asteraceae, especially lettuce crops. LMV isolates have previously been clustered in three main groups, LMV-Yar, LMV-Greek and LMV-RoW. The first two groups, LMV-Yar and LMV-Greek, have similar characteristics such as no seed-borne transmission and non-resistance-breaking. The latter one, LMV-RoW, comprising a large percentage of the LMV isolates contains two large subgroups, LMV-Common and LMV-Most. To date, however, no Korean LMV isolate has been classified and characterized. In this study, LMV-Muju, the Korean LMV isolate, was isolated from lettuce showing pale green and mottle symptoms, and its complete genome sequence was determined. Classification method of LMV isolates based on nucleotide sequence divergence of the NIb-CP junction showed that LMV-Muju was categorized as LMV-Common. LMV-Muju was more similar to LMV-O (LMV-Common subgroup) than to LMV-E (LMV-RoW group but not LMV-Common subgroup) even in the amino acid domains of HC-Pro associated with pathogenicity, and in the CI and VPg regions related to ability to overcome resistance. Taken together, LMV-Muju belongs to the LMV-Common subgroup, and is expected to be a seed-borne, non-resistance-breaking isolate. According to our analysis, all other LMV isolates not previously assigned to a subgroup were also included in the LMV-RoW group.

The behaviors of V2O5–WO3/TiO2 loaded on ceramic surfaces for NH3–SCR

Boxiong Shen,Fumei Wang,Bin Zhao,Yongwang Li,Yinyin Wang 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.33 No.-

The catalysts of V2O5–WO3/TiO2 loaded on ceramic surfaces were investigated to evaluate the behaviorsof catalytic activities, sulfur tolerance, K resistance, activities regeneration for K-poisoned catalyst andSi-modification in consideration the catalysts characterization. The results indicated that the high SCRactivities of these catalysts correlated with the large acid amount, the absorbed oxygen and the welldispersionof vanadium and tungsten on the surfaces. Washing the K-poisoned catalysts with sulfuricacid could partially recover the catalytic activity because of the K dissolution. Moreover, theincorporation of SiO2 with TiO2 considerably improved the resistance toward potassium because ofincreasing the acidity of the V2O5–WO3/TiO2 catalyst. The NO conversion increased from 26 to 68% for KpoisonedVW/TiO2(C-400) catalyst to 76–98% for K–VW/TiO2–SiO2(9)(C-400) catalyst at 260–410 8C. TheNH3 absorption of VW/TiO2–SiO2(6)(C-400) was 236.3 mmol/g, which was much higher than that of VW/TiO2(C-400) (73.3 mmol/g) (approximately 3.2 times higher).

Characteristics of a Lettuce mosaic virus Isolate Infecting Lettuce in Korea

임승모,임현섭,문제선,유란희,이수헌,Fumei Zhao,Davaajargal Igori 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.2

Lettuce mosaic virus (LMV) causes disease of plants in the family Asteraceae, especially lettuce crops. LMV isolates have previously been clustered in three main groups, LMV-Yar, LMV-Greek and LMV-RoW. The first two groups, LMV-Yar and LMV-Greek, have similar characteristics such as no seed-borne transmission and non-resistance-breaking. The latter one, LMV-RoW, comprising a large percentage of the LMV isolates contains two large subgroups, LMV-Common and LMV-Most. To date, however, no Korean LMV isolate has been classified and characterized. In this study, LMV-Muju, the Korean LMV isolate, was isolated from lettuce showing pale green and mottle symptoms, and its complete genome sequence was determined. Classification method of LMV isolates based on nucleotide sequence divergence of the NIb-CP junction showed that LMV-Muju was categorized as LMV-Common. LMV-Muju was more similar to LMV-O (LMV-Common subgroup) than to LMV-E (LMV-RoW group but not LMV-Common subgroup) even in the amino acid domains of HC-Pro associated with pathogenicity, and in the CI and VPg regions related to ability to overcome resistance. Taken together, LMV-Muju belongs to the LMV-Common subgroup, and is expected to be a seed-borne, non-resistance-breaking isolate. According to our analysis, all other LMV isolates not previously assigned to a subgroup were also included in the LMV-RoW group.

Genomic detection and characterization of a Korean isolate of Little cherry virus 1 sampled from a peach tree.

Lim, Seungmo,Igori, Davaajargal,Yoo, Ran Hee,Zhao, Fumei,Cho, In-Sook,Choi, Gug-Seoun,Lim, Hyoun-Sub,Lee, Su-Heon,Moon, Jae Sun M. Nijhoff ; Kluwer Academic Publishers 2015 Virus genes Vol.51 No.2

<P>A peach tree (Prunus persica) showing yellowing and mild mottle symptoms was analyzed using high-throughput RNA sequencing to determine the causal agent. A total of nine contigs similar to Little cherry virus 1 (LChV-1) were produced, and all the contigs showed nucleotide sequence identity (lower than 83??%) and query coverage (higher than 73??%) with LChV-1. The symptomatic peach sample was confirmed to be infected with LChV-1-like virus as a result of reverse transcription-polymerase chain reaction using primers designed based on sequences of the contigs. Occurrence of diseases caused by LChV-1 in Prunus species has been reported. Complete 16,931-nt genome of the peach virus composed of eight open reading frames was determined, and conserved domains including viral methyltransferase, viral helicase 1, RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homologue (HSP70h), HSP90h and closterovirus coat protein (CP) were identified. Phylogenetic trees based on amino acid sequence alignments between the peach virus and members in the family Closteroviridae showed that the virus was most similar to LChV-1. Pairwise comparisons based on amino acid sequence alignments of three genes (RdRp, HSP70h and CP) between the peach virus and LChV-1 isolates showed the highest amino acid sequence identities, with 84.32??% for RdRp, 85.48??% for HSP70h and 80.45??% for CP. These results indicate that this is the first report for the presence of LChV-1 in South Korea and may be one of the first reports of natural infection of peach by LChV-1. Although it is not clear if LChV-1 YD isolate was responsible for specific symptoms observed, detection and characterization of the peach tree-infecting LChV-1 in South Korea would be useful in terms of the epidemiology of LChV-1.</P>

Development of new vector by soybean yellow common mosaic virus for foreign gene expression and virus-induced gene silencing in soybean

Seungmo Lim,Jeong-Seon Kim,Moon Nam,Ran Hee Yoo,Fumei Zhao,Sang-Mok Kim,Yong-Gil Shin,Su-Heon Lee,Jae Sun Moon 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

In this study, we constructed viral vector for soybean by using Soybean yellow common mosaic virus (SYCMV) infecting both Glycine max and Glycine soja. SYCMV-derived viral vector was tested to use as Virus-induced gene silencing (VIGS) vector for functional analysis of soybean genes and as protein expression vector for foreign protein expression. In vitro transcript with 5’ cap analog m7GpppG from a full-length infectious vector of SYCMV driven by T7 promoter was inoculated to soybean to test infectivity of the clone (pSYCMVT7-full). 5’-capped transcript was able to infect soybean plants. The symptoms observed in soybean plants infected by either the vector or the sap from SYCMV-infected leaves were indistinguishable, suggesting that the vector had an equal biological activity shown by virus itself. To further utilize the vector, an additional DNA-based vector was constructed. The full-length cDNA was inserted into a binary vector flanked by CaMV 35S promoter and the nopaline synthase terminator (pSYCMV35S-full). To test if the vector infects soybean and subsequently induces gene silencing, we prepared two constructs containing fragments of Phytoene desaturase (PDS) gene (pSYCMV35S-PDS1) and small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS) gene (pSYCMV35S-rbcS2) from soybean plant. Plants infiltrated with the constructs through Agrobacterium-mediated method showed distinct symptoms such as photobleaching in plants infiltrated with pSYCMV-PDS1 and pale green or yellowing in plants infiltrated with pSYCMV-rbcS2. In addition, down-regulations of mRNA levels of two genes were confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). To test if the vector can be used for foreign protein expression in soybean plants, we prepared a construct encoding amino acids 135-160 of VP1 FMDV serotype O1 Campos (O1C) (pSYCMV35S-FMDV). Plants infiltrated with the construct through Agrobacterium-mediated method showed that soybean plant infiltrated with pSYCMV35S-FMDV only was detected by Western blotting using O1C antibody. These results support that SYCMV-derived viral vector can be used as VIGS vector or protein expression vector in soybean plants.