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Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China
Yan Zhang,Yali Lv,Feifei Zhang,Wenjing Zhang,Jinhong Wang,Yanyan Cui,Rongjun Wang,Fuchun Jian,Longxian Zhang,Changshen Ning 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.4
Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction(PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targetingthe 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0% (240/621) of sheep and 45.5% (323/710)of goats. The most frequently detected species was A. ovis (88/621, 14.2% for sheep; 129/710, 18.2% for goats), followed by A. bovis (60/621,9.7% for sheep; 74/710, 10.4% for goats) and A. phagocytophilum (33/621, 5.3% for sheep; 15/710, 2.1% for goats). Additionally, eight sheepand 20 goats were found to be infected with three pathogens simultaneously. DNA sequencing confirmed the presence of these three Anaplasmaspecies in the investigated areas, and phylogenetic analysis indicated that there was geographic segregation to a certain extent, as well as arelationship between the host and cluster of A. ovis. The results of the present study provide valuable data that helps understand theepidemiology of anaplasmosis in ruminants from China.