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- 저자 : Fifi Gus Dwiyanti (검색결과 1 건)
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Kusumadewi Yulita,Susila,Henti Hendalastuti Rachmat,Fifi Gus Dwiyanti,Tika D. Atikah,Atok Subiakto,Bayu Pratama,Titiek Setyawati,Wita Wardani,Rizki Ary Fambayun,Nawwall Arrofaha,Irsyad Kama 한국산림과학회 2022 Forest Science And Technology Vol.18 No.4
Dalbergia latifoliais commercial tropical tree species known for its beautiful heartwood,mainly used for furniture and musical instrument. High market demand has put concerns onits sustainability and conservation aspects in Indonesia. Ninety-five specimens ofD. latifoliawere collected from eight populations of Java, Lombok, and Sumbawa Island to study intra-specific variability and diversity using SRAP. One hundred and eighty SRAP loci with 54.03%± 4.35% polymorphism obtained from PCR amplification of 10 primer combinations, with theaverage PIC for these primers of 0.28. Genetic diversity and variability measures were calcu-lated using GenAlEx software indicating a relatively low-mid level of percentage of poly-morphic loci (PPL) (54.03%), effective number of alleles (Ne) (1.255), Shannon informationindex (I) (0.242), and heterozygosity (He) (0.156) on average. The highest value (I¼0.309, He¼0.196) was observed in population P2CJ (Central Java), followed by West Java of P1WJ(I¼0.300, He¼0.191) and West Sumbawa of P7Wsumb (I¼0.257, He¼0.169), while thelowest (I¼0.202, He¼0.129) was found in West Lombok (P4WL). The genetic relationshipswere measured from genetic distance and identity from the two-pairwise calculation, PCoA,and STRUCTURE analysis. The relative homologous population is found between populationsCentral and West Java of P2CJ and P1WJ (Nei uD¼0.031), suggesting these populationshave the same origin and formed one population, while the most distinct (Nei uD¼0.157)was recorded between Sumbawa regency (P6Sumb) and East Java (P3EJ). The results fromPCoA showed that all individuals are placed in three groups almost in accordance with theirrespective islands, i.e. a group comprised inclusively trees from Sumbawa island, a group ofLombok and some Central Java trees, and a group of trees from Java with the exclusion ofsome trees of Central Java that were clustered with trees from Lombok island. This result wasalso supported by STRUCTURE analysis, which showed that Sumbawa, Lombok, and West-East Java populations formed their respective clusters, and Central Java population contains amixture of West-East Java and Lombok populations. This may suggest evidence of complexorigin, thus needing further study to clarify. It is recommended that the populations with thehighest value of genetic diversity (Central and East Java) are to be maintained as the sourceof the gene pool for genetic enrichment programs.
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