Molecular Cloning and Bioinformatic Analysis of SPATA4 Gene

Liu, Shang-Feng,Ai, Chao,Ge, Zhong-Qi,Liu, Hai-Luo,Liu, Bo-Wen,He, Shan,Wang, Zhao Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6

Full-length cDNA sequences of four novel SPATA4 genes in chimpanzee, cow, chicken and ascidian were identified by bioinformatic analysis using mouse or human SPATA4 cDNA fragment as electronic probe. All these genes have 6 exons and have similar protein molecular weight and do not localize in sex chromosome. The mouse SPATA4 sequence is identified as significantly changed in cryptorchidism, which shares no significant homology with any known protein in swissprot databases except for the homologous genes in various vertebrates. Our searching results showed that all SPATA4 proteins have a putative conserved domain DUF1042. The percentages of putative SPATA4 protein sequence identity ranging from 30% to 99%. The high similarity was also found in 1 kb promoter regions of human, mouse and rat SPATA4 gene. The similarities of the sequences upstream of SPATA4 promoter also have a high proportion. The results of searching SymAtlas (http://symatlas.gnf.org/SymAtlas/) showed that human SPATA4 has a high expression in testis, especially in testis interstitial, leydig cell, seminiferous tubule and germ cell. Mouse SPATA4 was observed exclusively in adult mouse testis and almost no signal was detected in other tissues. The pI values of the protein are negative, ranging from 9.44 to 10.15. The subcellular location of the protein is usually in the nucleus. And the signal peptide possibilities for SPATA4 are always zero. Using the SNPs data in NCBI, we found 33 SNPs in human SPATA4 gene genomic DNA region, with the distribution of 29 SNPs in the introns. CpG island searching gives the data about CpG island, which shows that the regions of the CpG island have a high similarity with each other, though the length of the CpG island is different from each other.This research is a fundamental work in the fields of the bioinformational analysis, and also put forward a new way for the bioinformatic analysis of other genes.

Optimization Model of Reliable Data Storage in Cloud Environment Using Genetic Algorithm

Feng Liu,Haitao Wu,Xiaochun Lu,Xiyang Liu 보안공학연구지원센터 2014 International Journal of Grid and Distributed Comp Vol.7 No.6

Massive data storage is one of the great challenges for cloud computing service, and reliable storage of sensitive data directly affects quality of storage service. In this paper, based on analysis of data storage process in cloud environment, the cost of massive data storage is considered to be comprised of data storage price, data migration and communication; and the storage reliability consists of data transmission reliability and hardware dependability. A multi-objective optimization model for reliable massive storage is proposed, in which storage cost and reliability are the objectives. Then, a genetic algorithm for solving the model is designed. Finally, experimental results indicate that the proposed model is positive and effective.

Molecular Cloning and Bioinformatic Analysis of SPATA4 Gene

Shang Feng Liu,Chao Ai,Zhong Qi Ge,Hai Luo Liu,Bo Wen Liu,Shan He,Zhao Wang 한국생활환경학회 2005 BMB Reports Vol.38 No.6

Cloning and Functional Characterization of Putative Escherichia coli ABC Multidrug Efflux Transporter YddA

( Zhenyue Feng ),( Defu Liu ),( Ziwen Liu ),( Yimin Liang ),( Yanhong Wang ),( Qingpeng Liu ),( Zhenhua Liu ),( Zhongjing Zang ),( Yudong Cui ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.7

A putative multidrug efflux gene, yddA, was cloned from the Escherichia coli K-12 strain. A drugsensitive strain of E. coli missing the main multidrug efflux pump AcrB was constructed as a host and the yddA gene was knocked out in wild-type (WT) and drug-sensitive E. coliΔacrB to study the yddA function. Sensitivity to different substrates of WT E.coli, E. coliΔyddA, E. coliΔacrB and E. coliΔacrBΔyddA strains was compared with minimal inhibitory concentration (MIC) assays and fluorescence tests. MIC assay and fluorescence test results showed that YddA protein was a multidrug efflux pump that exported multiple substrates. Three inhibitors, ortho-vanadate, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and reserpine, were used in fluorescence tests. Ortho-vanadate and reserpine significantly inhibited the efflux and increased accumulation of ethidium bromide and norfloxacin, while CCCP had no significant effect on YddA-regulated efflux. The results indicated that YddA relies on energy released from ATP hydrolysis to transfer the substrates and YddA is an ABC-type multidrug exporter. Functional study of unknown ATP-binding cassette (ABC) superfamily transporters in the model organism E. coli is conducive to discovering new multidrug resistance-reversal targets and providing references for studying other ABC proteins of unknown function.

PARABOLIC MARCINKIEWICZ INTEGRALS ASSOCIATED TO POLYNOMIALS COMPOUND CURVES AND EXTRAPOLATION

Liu, Feng,Zhang, Daiqing Korean Mathematical Society 2015 대한수학회보 Vol.52 No.3

In this note we consider the parametric Marcinkiewicz integrals with mixed homogeneity along polynomials compound curves. Under the rather weakened size conditions on the integral kernels both on the unit sphere and in the radial direction, the $L^p$ bounds of such operators are given by an extrapolation argument. Some previous results are greatly extended and improved.

Numerical Design Method for Water-Lubricated Hybrid Sliding Bearings

Feng, Liu,Bin, Lin,Xiaofeng, Zhang Korean Society for Precision Engineering 2008 International Journal of Precision Engineering and Vol.9 No.1

This paper presents a new water-lubricated hybrid sliding bearing for a high speed and high accuracy main shaft system, along with the numerical method used for its design. The porous material for the restrictor and the restriction parameter were chosen based on the special requirements of the water-lubricated bearing. Subsequent numerical calculations give the load capacity, stiffness, and friction power of different forms of water-lubricated bearings. The pressure distribution of the water film in a 6-cavity bearing is shown, based on the results of the numerical calculations. A comparison of oil-lubricated and water-lubricated bearings shows that the latter benefits more from improved processing precision and efficiency. An analysis of the stiffness and friction power results shows that 6-cavity bearings are the preferred type, due their greater stiffness and lower friction power. The average elevated temperature was calculated and found to be satisfactory. The relevant parameters of the porous restrictor were determined by calculating the restriction rate. All these results indicate that this design for a water-lubricated bearing meets specifications for high speed and high accuracy.

PARABOLIC MARCINKIEWICZ INTEGRALS ASSOCIATED TO POLYNOMIALS COMPOUND CURVES AND EXTRAPOLATION

Feng Liu,Daiqing Zhang 대한수학회 2015 대한수학회보 Vol.52 No.3

In this note we consider the parametric Marcinkiewicz integrals with mixed homogeneity along polynomials compound curves. Under the rather weakened size conditions on the integral kernels both on the unit sphere and in the radial direction, the Lp bounds of such operators are given by an extrapolation argument. Some previous results are greatly extended and improved.

Parallel Distributed Acceleration Based on MPI and OpenMP Technology

Feng Liu,Haitao Wu,Xiaochun Lu,Xiyang Liu 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.6

In order to speed up data processing in a signal monitoring and evaluation system, we need to use a parallel method. It is obvious that the traditional stand-alone store has no ability to satisfy the performance requirements, and the use of single core CPU is unable to content the severe requirement of speed. Consequently, multi-machine parallel acceleration technique based on MPI (cooperated with multi-core parallel acceleration technique based on OpenMP) can effectively solve all above problems. In this paper, a parallel distributed acceleration framework based on MPI and Open MP technology was given. Experimental tests were carried to verify our proposal. Finally, some suggestions to speed up the data processing was given.

MTHFR C677T Polymorphism and Pancreatic Cancer Risk: a Meta-analysis

Liu, Xiang-Ming,Liu, Feng-Hua,Tang, Yong,Li, Qiang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in the metabolism of folate, and the role of the MTHFR C677T polymorphism in pancreatic carcinogenesis is still controversial. Methods: A literature search was performed using Pubmed and CNKI databases for published studies through May 2012. We performed a meta-analysis of all relevant case-control studies that examined the association between MTHFR C677T polymorphism and pancreatic cancer risk. Results: Finally, 9 individual case-control studies with a total of 1,299 pancreatic cancer cases and 2,473 controls were included into this meta-analysis. Results: This metaanalysis showed there was an obvious association between MTHFR C677T polymorphism and pancreatic cancer risk in East Asians (for allele model, OR = 1.67, 95%CI 1.11-2.51; For homozygote model, OR = 2.77, 95%CI 1.40-5.48; for recessive model, OR = 1.96, 95%CI 1.54-2.50; for dominant model, OR = 2.11, 95%CI 1.01-4.41). However, no significant association was found in Caucasians. Conclusions: The MTHFR C677T polymorphism is associated with pancreatic cancer risk, and a race-specific effect may exist in this association. More studies with a larger sample size are needed to further clarify this association.

Memetic Two-echelon Vehicle Routing Optimization Based on Q Learning Theory and Differential Evolution Algorithm

Liu Dongdong,Liu Kai,Wang Feng,Han Bo,Zhao Zhengping,Tan Fuxiao,Niu Lei 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.8

In allusion to such problems as low accuracy and long convergence time in traditional two-echelon vehicle routing optimization algorithm, a Memetic algorithm (QDEMA) based on Q learning theory and differential evolution is proposed in this article to solve above problems. Firstly, it is necessary to research the two-echelon vehicle routing optimization problem and adopt the optimal segmentation method to obtain the relatively reasonable distribution plan for the first-echelon SDVRP problem in order to accordingly determine the distribution quantity of the transfer stations; secondly, the second-echelon MDVRP distribution scheme is solved to obtain the total distance and the total number of the distribution vehicles for the two-echelon optimization problem; thirdly, in allusion to the solution of the second-echelon MDVRP distribution scheme, Q learning theory and the differential evaluation algorithm are adopted to design new Memetic algorithm in order to globally optimize MDVRP distribution scheme; finally, the simulation experiment is carried out to verify the algorithm effectiveness.